1. Antigenic protein modifications inEhrlichia
- Author
-
Sunil Thomas, Nahed Ismail, David H. Walker, Nagaraja R. Thirumalapura, and Emily C. Crossley
- Subjects
Proteome ,Immunology ,Ehrlichia ,Priming (immunology) ,Eastern blotting ,Mice ,03 medical and health sciences ,antigen ,IOE ,Immune system ,Bacterial Proteins ,Antigen ,Animals ,Electrophoresis, Gel, Two-Dimensional ,030304 developmental biology ,Antigens, Bacterial ,0303 health sciences ,Ixodes ,biology ,030306 microbiology ,Original Articles ,Eastern blot ,biology.organism_classification ,Molecular biology ,3. Good health ,Mice, Inbred C57BL ,ehrlichiosis ,Polyclonal antibodies ,biology.protein ,Parasitology ,Antibody ,Protein Processing, Post-Translational - Abstract
To develop effective vaccination strategies against Ehrlichia, we have previously reported developing an animal model of cross-protection in which C57BL/6 mice primed with E. muris were resistant to lethal infection with Ixodes ovatus ehrlichia (IOE). Polyclonal antibody produced in mice after priming with E. muris and later injected with IOE-detected antigenic proteins in E. muris and IOE cell lysates. Cross-reaction of antigenic proteins was observed when we probed both the E. muris and IOE cell lysates with IOE and E. muris-specific polyclonal antibody. Analysis of the total proteins of E. muris and IOE by two dimensional electrophoresis showed that both E. muris and IOE have the same antigenic proteins. Finally, studies on post-translational protein modifications using a novel technique, Eastern blotting, showed that E. muris proteins are more lipoylated and glycosylated than those of IOE.
- Published
- 2009
- Full Text
- View/download PDF