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Abstract The inverse temperature layer (ITL) beneath water‐atmosphere interface within which temperature increases with depth has been observed from measurement of water temperature profile at an inland lake. Strong solar radiation combined with moderate wind‐driven near‐surface turbulence leads to the formation of a pronounced diurnal cycle of the ITL predicted by a physical heat transfer model. The ITL only forms during daytime when solar radiation intensity exceeds a threshold while consistently occurs during nighttime. The largest depth of the ITL is comparable to the e‐fold penetration depth of solar radiation during daytime and at least one order of magnitude deeper during nighttime. The dynamics of the ITL depth variation simulated by a physical model forced by observed water surface solar radiation and temperature is confirmed by the observed water temperature profile in the lake.