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3 results on '"Minglin Ou"'

1. Identification and Validation of Immune-Related Gene Prognostic Signature for Hepatocellular Carcinoma

Author

Wenbiao Chen, Minglin Ou, Donge Tang, Yong Dai, and Weibo Du

Subjects
Immunologic diseases. Allergy, RC581-607
Abstract

Immune-related genes (IRGs) have been identified as critical drivers of the initiation and progression of hepatocellular carcinoma (HCC). This study is aimed at constructing an IRG signature for HCC and validating its prognostic value in clinical application. The prognostic signature was developed by integrating multiple IRG expression data sets from TCGA and GEO databases. The IRGs were then combined with clinical features to validate the robustness of the prognostic signature through bioinformatics tools. A total of 1039 IRGs were identified in the 657 HCC samples. Subsequently, the IRGs were subjected to univariate Cox regression and LASSO Cox regression analyses in the training set to construct an IRG signature comprising nine immune-related gene pairs (IRGPs). Functional analyses revealed that the nine IRGPs were associated with tumor immune mechanisms, including cell proliferation, cell-mediated immunity, and tumorigenesis signal pathway. Concerning the overall survival rate, the IRGPs distinctly grouped the HCC samples into the high- and low-risk groups. Also, we found that the risk score based on nine IRGPs was related to clinical and pathologic factors and remained a valid independent prognostic signature after adjusting for tumor TNM, grade, and grade in multivariate Cox regression analyses. The prognostic value of the nine IRGPs was further validated by forest and nomogram plots, which revealed that it was superior to the tumor TNM, grade, and stage. Our findings suggest that the nine-IRGP signature can be effective in determining the disease outcomes of HCC patients.

Published
2020
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2. CCDC40 mutation as a cause of primary ciliary dyskinesia: a case report and review of literature

Author

Guoping Sun, Xianliang Hou, Shengxing Huang, Xiaolian Wei, Peng Chen, Fuhua Liu, Minglin Ou, Weiguo Sui, Yong Dai, and Wenti Che

Subjects
0301 basic medicine, Pulmonary and Respiratory Medicine, Infertility, Pathology, medicine.medical_specialty, Genetic heterogeneity, Cilium, Biology, Gene mutation, medicine.disease, Male infertility, 03 medical and health sciences, Situs inversus, 030104 developmental biology, otorhinolaryngologic diseases, medicine, Immunology and Allergy, Genetics (clinical), Exome sequencing, Primary ciliary dyskinesia
Abstract

Background and Aims Primary ciliary dyskinesia (PCD) is a rare, genetically heterogeneous disorder. Genetic defects affecting motility of cilia and flagella cause chronic destructive airway disease, situs inversus and, frequently, male infertility in PCD. To date, although several genes have been implicated in PCD, the genetic bases of most cases of PCD remain elusive. Methods By applying a whole-exome sequencing strategy, we reported a case of PCD carrying a novel mutant alleles in CCDC40 gene, and did literature review. Results A 36-year-old nonsmoking Chinese man suffered from chronic cough since childhood and an 8-year history of primary infertility. Lung biopsy showed respiratory bronchiolitis. Chest images showed bronchiectasis and situs inversus. Semen analysis showed high sperm counts and poor sperm motility. Transmission electron microscopy (TEM) of cilia cross-sections showed ultrastructural defects, including inner dynein arms (IDA) defect and axonemal disorganization. To identify gene mutations that cause PCD, we performed exome sequencing to analyze genome of this patient, and discovered a previously uncharacterized mutant alleles (NM_001243342.1:c.2609G>A; p. R870H) in CCDC40 gene. In addition, we summarize the PCD disease-causing genes and CCDC40 mutant sites based on current literature. Conclusions We identified a novel mutant alleles in CCDC40 gene, which altered the protein sequence and resulted in the ultrastructural defects in the microtubule structure of cilia. Thereby, these defects lead to the patient with bronchiectasis, bronchiolitis and infertility.

Published
2015

3. Comprehensive analysis of transcription factor expression patterns in peripheral blood mononuclear cell of systemic lupus erythematosus

Author

Minglin Ou, Jiejing Chen, Yong Dai, Hua Lin, and Weiguo Sui

Subjects
Microarray, genetic processes, Electrophoretic Mobility Shift Assay, Biology, Rheumatology, immune system diseases, medicine, Humans, Lupus Erythematosus, Systemic, natural sciences, Electrophoretic mobility shift assay, skin and connective tissue diseases, Transcription factor, Oligonucleotide Array Sequence Analysis, Regulation of gene expression, Lupus erythematosus, Systemic lupus erythematosus, Gene Expression Profiling, fungi, Autoantibody, medicine.disease, Gene expression profiling, Gene Expression Regulation, Immunology, Leukocytes, Mononuclear, Transcription Factors
Abstract

Background Transcription factors (TFs) play a central role in regulating gene expression and in providing interconnecting regulatory networks between related pathway elements. Systemic lupus erythematosus (SLE) is an organ-nonspecific autoimmune disorder characterized by the production of autoantibodies against a host of nuclear antigens. Aim The pathogenesis of lupus is incompletely understood. Understanding the mechanisms that contribute to SLE and finding effect biomarkers to anticipate SLE will be of great value. Method To investigate possible mechanisms, we describe a comparison of TF activity profiles between SLE and controls. Through TF assay analysis and electrophoretic mobility shift assay (EMSA) confirmation, we identified different activities of TFs in SLE. Results Three hundred and forty-five TFs were detected in both groups, with 92 of them differentially expressed by TF array in which 78 TFs up-regulated and 14 TFs down-regulated in SLE compared with the control group, while 253 TFs showed no significant expression levels. The array data was consistent with the EMSA verification results. Conclusion Our data indicated that TFs may be potentially involved in the pathogenesis of SLE, and can help to diagnose, treat and prevent SLE. The method could simplify the assay of multiple TFs and may facilitate high-throughput profiling of large numbers of TFs.

Published
2012

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