1. Identification of human low-density lipoprotein receptor as a novel target gene regulated by liver X receptor alpha
- Author
-
Kenji Ishimoto, Yuichiro Watanabe, Tatsuhiko Kodama, Takefumi Doi, Ikuko Hanano, Mikako Sumitomo, Keisuke Tachibana, Daisuke Yamasaki, Shiho Omote, Juro Sakai, Takao Hamakubo, and Toshiya Tanaka
- Subjects
medicine.medical_specialty ,Low-density lipoprotein receptor gene family ,Biophysics ,Receptors, Cytoplasmic and Nuclear ,Biology ,Retinoid X receptor ,SREBP ,Biochemistry ,Liver X receptor beta ,Structural Biology ,Cell Line, Tumor ,Internal medicine ,Genetics ,medicine ,Animals ,Humans ,Low-density lipoprotein receptor ,Liver X receptor ,Promoter Regions, Genetic ,Molecular Biology ,Liver X Receptors ,Retinoid X Receptor alpha ,Retinoid X receptor alpha ,Liver receptor homolog-1 ,Liver X receptor alpha ,Cell Biology ,Orphan Nuclear Receptors ,Cell biology ,DNA-Binding Proteins ,Cholesterol ,Endocrinology ,Gene Expression Regulation ,Receptors, LDL ,Nuclear receptor ,LDL receptor ,lipids (amino acids, peptides, and proteins) ,LXR response element ,Dimerization - Abstract
Liver X receptor alpha (LXRalpha) is a member of the nuclear receptor superfamily that is activated by oxysterols, and plays a pivotal role in regulating the metabolism, transport and uptake of cholesterol. Here, we demonstrate that LXRalpha also regulates the low-density lipoprotein receptor (LDLR) gene, which mediates the endocytic uptake of LDL cholesterol in the liver. An LXR agonist induced the expression of LDLR in cultured hepatoblastoma cells. Moreover, the LDLR promoter contained an LXR response element that was recognized by LXRalpha/RXRalpha (retinoid X receptor alpha) heterodimers in hepatoblastoma cells. These results suggest a novel pathway whereby LXRalpha might modulate cholesterol metabolism.
- Published
- 2006
- Full Text
- View/download PDF