1. Optimization of integrated chromatography sequences for purification of biopharmaceuticals
- Author
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Anton Löfgren, Niklas Andersson, Mikael Yamanee-Nolin, Simon Tallvod, Bernt Nilsson, and Joaquin Gomis Fons
- Subjects
0106 biological sciences ,Biological Products ,Chromatography ,Computer science ,business.industry ,010401 analytical chemistry ,Scheduling (production processes) ,Antibodies, Monoclonal ,01 natural sciences ,0104 chemical sciences ,Nonlinear programming ,010608 biotechnology ,Staphylococcal Protein A ,Process engineering ,business ,Countercurrent Distribution ,Biotechnology - Abstract
With continued development of integrated and continuous downstream purification processes, tuning and optimization become increasingly complicated with additional parameters and codependent variables over the sequence. This article offers a novel perspective of nonlinear optimization of integrated sequences with regard to individual column sizes, flow rates, and scheduling. The problem setup itself is a versatile tool to be used in downstream design which is demonstrated in two case studies: a four-column integrated sequence and a continuously loaded twin-capture setup with five columns.
- Published
- 2019
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