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Your search keyword '"Michele Scorzeto"' showing total 2 results
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2 results on '"Michele Scorzeto"'

1. The synaptotagmin juxtamembrane domain is involved in neuroexocytosis

Author

Paola Caccin, Michele Scorzeto, Nunzio Damiano, Oriano Marin, Aram Megighian, and Cesare Montecucco

Subjects
Synaptotagmin, Juxtamembrane domain, Anionic phospholipids, Neuromuscular junction, Neuroexocytosis, Biology (General), QH301-705.5
Abstract

Synaptotagmin is a synaptic vesicle membrane protein which changes conformation upon Ca2+ binding and triggers the fast neuroexocytosis that takes place at synapses. We have synthesized a series of peptides corresponding to the sequence of the cytosolic juxtamembrane domain of synaptotagmin, which is highly conserved among different isoforms and animal species, with or without either a hexyl hydrophobic chain or the hexyl group plus a fluorescein moiety. We show that these peptides inhibit neurotransmitter release, that they localize on the presynaptic membrane of the motor axon terminal at the neuromuscular junction and that they bind monophosphoinositides in a Ca2+‐independent manner. Based on these findings, we propose that the juxtamembrane cytosolic domain of synaptotagmin binds the cytosolic layer of the presynaptic membrane at rest. This binding brings synaptic vesicles and plasma membrane in a very close apposition, favouring the formation of hemifusion intermediates that enable rapid vesicle fusion.

Published
2015
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2. Age-dependent neuromuscular impairment in prion protein knockout mice

Author

Carlo Reggiani, Michele Scorzeto, Federica Loro, Maria Lina Massimino, Bert Blaauw, Caterina Peggion, Aram Megighian, Roberto Stella, Maria Catia Sorgato, Alessandro Bertoli, and Luana Toniolo

Subjects
0301 basic medicine, medicine.medical_specialty, Physiology, animal diseases, Age dependent, Biology, 03 medical and health sciences, Cellular and Molecular Neuroscience, 0302 clinical medicine, Physiology (medical), Internal medicine, medicine, Aerobic exercise, Prion protein, Treadmill, Skeletal muscle, nervous system diseases, Genetically modified organism, 030104 developmental biology, medicine.anatomical_structure, Endocrinology, Knockout mouse, Neurology (clinical), Sciatic nerve, Neuroscience, 030217 neurology & neurosurgery
Abstract

INTRODUCTION The cellular prion protein (PrP(C) ) is commonly recognized as the precursor of prions, the infectious agents of the fatal transmissible spongiform encephalopathies, or prion diseases. Despite extensive effort, the physiological role of PrP(C) is still ambiguous. Evidence has suggested that PrP(C) is involved in different cellular functions, including peripheral nerve integrity and skeletal muscle physiology. METHODS We analyzed the age-dependent influence of PrP(C) on treadmill test-based aerobic exercise capacity and on a series of morphological and metabolic parameters using wild-type and genetically modified mice of different ages expressing, or knockout (KO) for, PrP(C) . RESULTS We found that aged PrP-KO mice displayed a reduction in treadmill performance compared with PrP-expressing animals, which was associated with peripheral nerve demyelination and alterations of skeletal muscle fiber type. CONCLUSION PrP-KO mice have an age-dependent impairment of aerobic performance as a consequence of specific peripheral nerve and muscle alterations.

Published
2015
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