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ABSTRACT Objective Sodium hypochlorite solution (NaOCl) is an effective canal irrigant but interferes with the mechanical features of dentin and the bonding capability of adhesives when restoring endodontically treated teeth. This study evaluated whether access cavity resin sealing before using canal irrigant would augment the resistance of endodontically treated anterior teeth against fracture. Methods Sixty maxillary incisors underwent endodontic treatment in five groups (n = 12). Irrigation with 5.25% NaOCl and 17% ethylenediaminetetraacetic acid (EDTA) was performed in all groups except for Group 5. After root canal obturation, in Group 1, the access cavity was kept unrestored. In Group 2, immediate restoration after obturation was achieved. For Group 3, delayed restoration after 1 week was provided. In Group 4 (pre‐sealed), before canal irrigation, the dentin surface of access cavities was sealed using self‐adhesive composite resin (Vertise Flow) and then restored after obturation. In Group 5, which was saline irrigated, immediate restoration was performed. After storage and thermal cycling for 5000 cycles at 5°C–55°C with a dwell time of 15 s and a transfer time of 5 s, teeth were statically loaded by a universal testing machine until a fracture occurred. Data were collected as the fracture resistance (FR) and analyzed using the one‐way analysis of variance and Tukey's tests. Results FR significantly differed between all groups (p

Key message The visualization of black pigment during EBUS‐TBNA suggests a relapse of melanoma. This case highlights the value of EBUS‐TBNA in diagnosing metastatic melanoma, particularly when the macroscopic appearance of the aspirate suggests the diagnosis.

Abstract Objective This study evaluated the effect of two natural antioxidants on the compromised bond strength of a resin‐modified glass ionomer (RMGI) to the sodium hypochlorite (NaOCl)‐affected pulp chamber dentin. Methods Forty‐two sound third molars were split into halves. The exposed pulp chamber dentin was ground to provide the flat dentin surfaces and divided into seven groups (n = 12), according to the solutions used for immersion: (1) Control, distilled water; (2) NaOCl, 5.25% NaOCl for 20 min; (3) NaOCl/Ethylenediaminetetraacetic acid (EDTA); 5.25% NaOCl for 20 min + 17% EDTA for 1 min; (4) NaOCl/TA, 5.25% NaOCl + 10% tanic acid (TA) for 5 min; (5) NaOCl/EDTA/TA, 5.25% NaOCl + 17% EDTA + 10% TA for 5 min; (6) NaOCl/PA, 5.25% NaOCl+ 10% proanthocyanidin for 5 min; and (7) NaOCl/EDTA/PA, 5.25% NaOCl+ 17% EDTA + 10% PA for 5 min. The RMGI was bonded on the treated dentin using a Tygon tube. After 24 h of storage, microshear bond strength (µSBS) was tested. Data in MPa were submitted to one‐way analysis of variance and Tamhane test. Results NaOCl significantly decreased the µSBS; NaOCl/EDTA and NaOCl/TA significantly increased the µSBS, higher than the control group (p .05). NaOCl/PA and NaOCl/EDTA/PA and NaOCl groups had comparable µSBS. Conclusion TA could be suggested to provide effective bonding of RMGI and immediate sealing of the pulp chamber dentin after NaOCl irrigation.

Abstract Objective Given the importance of preserving caries‐affected dentin (CAD) in conservative dentistry, the shear bond strength (SBS) of different resin cements to CAD has been investigated. Here, we aimed to compare the SBS and remineralizing effect of a calcium silicate (TheraCem) and conventional self‐adhesive cement (Panavia SA) on the SBS of CAD. Materials and Methods Forty‐eight extracted third molars (24 sound and 24 CAD) were used. In each group, 12 teeth were prepared for bonding to TheraCem or Panavia SA. After removal of the enamel and caries, resin composite cylinders were luted on the prepared dentin. After 28 days of storage in the artificial saliva, SBS was measured and the failure mode analysis was investigated. The images of fractured sections were analyzed using scanning electron microscopy and energy‐dispersive X‐ray to evaluate the Ca/P weight ratio. Results SBS of CAD and sound dentin was not different when cemented with TheraCem (9.56 ± 4.51 vs. 9.17 ± 2.76, p = .806), but the CAD showed significantly lower SBS to Panavia SA (9.4 ± 2.36 vs. 7.39 ± 2.18, p = .015). The Ca/P ratio in CAD was significantly higher when bonded to both TheraCem and Panavia‐SA than that of the controls (p = .001); however, this ratio was not different for those bonded to TheraCem compared to Panavia SA. Conclusions Based on our results, TheraCem as a calcium silicate cement shows better SBS to attach the restoration to CAD as compared to Panavia SA. Obliteration and mineralization of the dentinal tubules in TheraCem were also higher than in Panavia SA. However, their ability to improve the amount of the Ca/P ratio in CAD was similar.

Abstract Objective This study aimed to investigate the effects of bleaching on the color, translucency, surface roughness, and surface hardness of monolithic zirconia with external stainin . Methods In this experimental study, 32 specimens of monolithic zirconia (1 × 1 mm; shade A2) were divided into two groups based on random permuted blocks. Overglaze and staining procedures were performed with a yellow stain or a value stain (GC Stain). Baseline color, translucency, roughness, and surface hardness were measured. The specimens were then randomly bleached with hydrogen peroxide (HP) 40% (20 min, twice with a 1‐week interval in between) as office bleaching or carbamide peroxide (CP) 20% (4 h per day for 14 days) as home bleaching. Finally, the color, translucency, surface roughness, and surface hardness were measured again. Results Bleaching with CP and HP caused a perceptible change in the color of the specimens (ΔE > 2), although this change was within the clinically acceptable range (ΔE

Abstract An efficient technique for calculating the scattering from curved metasurfaces using the extinction theorem in conjunction with the Floquet and Fourier series expansions is presented. Here, we treat the two‐dimensional metasurfaces that have transversal polarizabilities with no variation along the y‐axis. The boundary conditions at the metasurface are given by the generalized sheet transition conditions (GSTCs) whose susceptibilities are given in an arbitrary local coordinate system. First, we use the extinction theorem to provide integral equations of the scattering problem. The integral equations involve the Green's functions, tangential electric and magnetic fields and their normal derivatives in regions above and below the metasurface. Then, we employ the Floquet theorem that gives us the analytical periodic Green's functions of each region. Next, we employ the Fourier theorem to expand the tangential fields in terms of unknown Fourier coefficients. The GSTCs and the integral equations provide equations to be solved for the unknowns. The method can calculate scattering from both periodic and non‐periodic metasurfaces. The technique is used to analyse different applied problems such as carpet cloaking, illusion, and radar echo width reduction. The method is fast and accurate and can efficiently treat metasurfaces with electrically large curved geometries with dimensions as large as 120 times the wavelength.

Abstract Introduction The stem cell portion of the dental pulp derived cultures (DPSCs) showed a higher resistance to cytotoxic effect of restorative dental materials compared to pulpal fibroblasts (DPFs). Here, we aimed to compare the expression of some drug resistant genes between these cells. Methods and materials To separate DPSCs from DPFs, we used magnetic cell sorting technique based on CD146 expression. To assess the stem cell properties, the positive and negative portions underwent colony forming assays and were induced to be differentiated into the adipocytes, osteoblasts, hepatocytes, and neural cells. Cell surface antigen panels were checked using immune fluorescence and flow‐cytometry techniques. The mRNA expression of 14 ABC transporters including ABCA2, ABCB1, ABCB11, ABCC1, ABCC2, ABCC3, ABCC4, ABCC5‐2, ABCC5‐4,ABCC5‐13, ABCC6, ABCC10, ABCC11, and ABCG2 genes was assessed, using quantitative RT‐PCR technique. Results Only the CD146 positive portion could be differentiated into the desired fates, and they formed higher colonies (16.7 ± 3.32 vs. 1.7 ± 1.67, p

Abstract Background and Aims All components of the immune system are involved in alleviating severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) infection. Further research is required to provide detailed insights into COVID‐19‐related immune compartments and pathways. In addition, a significant percentage of hospitalized COVID‐19 patients suspect bacterial infections and antimicrobial resistance occurs following antibiotics treatment. The aim of this study was to evaluate the possible effects of antibiotics on the response of neutrophil‐related genes in SARS‐CoV‐2 patients by an experimental in silico study. Methods The two data sets GSE1739 and GSE21802 including 10 SARS positive patients and 35 influenza A (H1N1) patients were analyzed, respectively. Differentially expressed genes (DEGs) between these two data sets were determined by GEO2R analysis and the Venn diagram online tool. After determining the hub genes involved in immune responses, the expression of these genes in 30 COVID‐19 patients and 30 healthy individuals was analyzed by real‐time polymerase chain reaction (PCR). All patients received antibiotics, including levofloxacin, colistin, meropenem, and ceftazidime. Results GEO2R analysis detected 240 and 120 DEGs in GSE21802 and GSE1739, respectively. Twenty DEGs were considered as enriched hub genes involved in immune processes such as neutrophil degranulation, neutrophil activation, and antimicrobial humoral response. The central nodes were attributed to the genes of neutrophil elastase (ELANE), arginase 1 (ARG‐1), lipocalin 2 (LCN2), and defensin 4 (DEFA4). Compared to the healthy subjects, the expression of LCN2 and DEFA4 were significantly reduced in COVID‐19 patients. However, no significant differences were observed in the ELANE and AGR‐1 levels between COVID‐19 subjects and the control group. Conclusions Activation and degranulation of neutrophils were observed mainly in SARS, and H1N1 infection processes and antibiotics administration could affect neutrophil activity during viral infection. It can be suggested that antibiotics can decrease inflammation by restoring the expression of neutrophil‐related genes in COVID‐19 patients.

Abstract Introduction Sleep disturbances are common in Alzheimer's disease (AD), with estimates of prevalence as high as 65%. Recent work suggests that specific sleep stages, such as slow‐wave sleep (SWS) and rapid eye movement (REM), may directly impact AD pathophysiology. A major limitation to sleep staging is the requirement for clinical polysomnography (PSG), which is often not well tolerated in patients with dementia. We have recently developed a deep learning model to reliably analyze lower quality electroencephalogram (EEG) data obtained from a simple, two‐lead EEG headband. Here we assessed whether this methodology would allow for home EEG sleep staging in patients with mild–moderate AD. Methods A total of 26 mild–moderate AD patients and 24 age‐matched, healthy control participants underwent home EEG sleep recordings as well as actigraphy and subjective sleep measures through the Pittsburgh Sleep Quality Index (PSQI). Each participant wore the EEG headband for up to three nights. Sleep was staged using a deep learning model previously developed by our group, and sleep stages were correlated with actigraphy measures as well as PSQI scores. Results We show that home EEG with a headband is feasible and well tolerated in patients with AD. Patients with mild–moderate AD were found to spend less time in SWS compared to healthy control participants. Other sleep stages were not different between the two groups. Actigraphy or the PSQI were not found to predict home EEG sleep stages. Discussion Our data show that home EEG is well tolerated, and can ascertain reduced SWS in patients with mild–moderate AD. Similar findings have previously been reported, but using clinical PSG not suitable for the home environment. Home EEG will be particularly useful in future clinical trials assessing potential interventions that may target specific sleep stages to alter the pathogenesis of AD. Highlights Home electroencephalogram (EEG) sleep assessments are important for measuring sleep in patients with dementia because polysomnography is a limited resource not well tolerated in this patient population. Simplified at‐home EEG for sleep assessment is feasible in patients with mild–moderate Alzheimer's disease (AD). Patients with mild–moderate AD exhibit less time spent in slow‐wave sleep in the home environment, compared to healthy control participants. Compared to healthy control participants, patients with mild–moderate AD spend more time in bed, with decreased sleep efficiency, and more awakenings as measured by actigraphy, but these measures do not correlate with EEG sleep stages.

ABSTRACT Introduction Neonatal diabetes mellitus (NDM) is a rare non‐immunological monogenic disorder characterized by hyperglycemic conditions primarily occurring within the first 6 months of life. The majority of cases are attributed to pathogenic variants in genes affecting beta‐cell survival, insulin regulation, and secretion. This study aims to investigate the genetic landscape of NDM in Iran. Methods We recruited a total of 135 patients who were initially diagnosed with diabetes at

Abstract Biomolecules with metals can form supramolecular nanomaterials through coordination assembly, opening new avenues for cancer theranostics and bringing unique insights into personalized nanomedicine. These biomaterials have been considered versatile and innovative nanoagents due to their structure‒function control, biological nature, and simple preparation methods. This review article summarized the recent developments in multicomponent nanomaterials formed from metal coordination interactions with amino acids, peptides, and proteins, together with anticancer drugs, for cancer theranostics. We discussed the role of functional groups anchored in building blocks for coordination interactions, and subsequently, the types of interactions were examined from a structure‒function perspective. Amino acids with different metals and anticancer drugs forming supramolecular nanomaterials and their anticancer mechanisms were highlighted. Peptides with different metals and anticancer drugs, proteins with metals and anticancer drugs used for material formations, and anticancer activity have been discussed. Ultimately, the conclusion and future outlook for multicomponent supramolecular nanomaterials offer fundamental insights into fabrication design and precision medicine.

ABSTRACT Background and Aims Menopause is a multifaceted condition with several problems. Educational interventions regarding self‐care and limiting exposure to menopausal warning indicators can help to minimize problems. As a result, the study aimed to investigate the influence of educational intervention on variables influencing menopausal adaptation. Methods This study was a randomized controlled trial (RCT) intervention in which the researcher developed a standard questionnaire (kapa = o/99, CVR = 0/81, CVI = 0/96, ICC = 0/89) for 130 participants at four education sessions in Mashhad, Iran, in 2023. This questionnaire was completed before the intervention, shortly afterward, and 3 months later. Results The average age was 52 ± 4 years, with no significant difference between the two groups. According to the regression model findings immediately following the intervention, the factors of education level, income level, and age had no significant influence on the overall score of the questionnaire. The linear regression model revealed that the intervention group had a higher average score of 13.89. The outcomes 3 months after the intervention were identical to those immediately following the intervention. Three months after the intervention, there was a substantial change in the score for encountering warning signals and seeking help and self‐care compared to before (p‐value = 0.002, p‐value

ABSTRACT Aim This study evaluated surgical complication rates, recurrence‐free survival, overall survival (OS), and stoma status of patients with rectal cancer after significant pathologic response following neoadjuvant treatment and curative resection. Pathologic complete response (pCR) and near‐pCR patients constitute patients in our study. Methods Included was a retrospective cohort study of patients with rectal cancer who were diagnosed between July 2011 and September 2022 and who underwent neoadjuvant therapy and surgical resection. Results Of 696 patients with rectal cancer, 149 (21.4%) cases achieved significant pathologic response. During the 64 (70.5) months of follow‐up, recurrence occurred in 16.1% of patients and distant metastases account for the majority of them. Age (p = 0.014) and receiving adjuvant chemotherapy (p = 0.016) were significantly related to the occurrence of recurrence. The five‐year recurrence‐free survival (RFS) and OS rates were obtained at 83% and 87%, respectively. Although age and surgical technique were significant factors in univariate Cox regression analysis, none of the candidate variables were significant prognostic factors for RFS in the multiple models. The risk of surgical complications remained in these patients. The most frequent complication attributed to infection (20.8%). Despite the 24.8% presence of permanent stoma at primary surgery, more than 50% of our patients lived without stoma at the last follow‐up. Conclusion Our recurrence rate was about 16%, and it was related to age and adjuvant chemotherapy. These patients achieved over 80% rates of five‐year RFS and OS. No significant prognostic factors were found on RFS in the multivariable model. As a matter of course, the risk of surgical complications and permanent stoma has still remained in these patients.

Abstract Purpose During the transportal technique of anterior cruciate ligament (ACL) reconstruction, tunnel outlet location can be varied depending on certain anatomical and technical characteristics. Therefore, we aimed to find out the acceptable zone of endobutton location by introducing several radiographic values. Methods Postoperative lateral radiographs of 72 patients were assessed to measure the distances from the centre of the button to the posterior femoral cortex (D1) and to the most distal point of the lateral condyle (D2). Furthermore, based on the anteroposterior (AP) radiographs, the distances from the centre of the button to the lateral femoral cortex (D3) and from the centre of the button to the line connecting the most distal points of the medial and lateral femoral condyles (D4) were assessed. To measure the sensitivity and specificity of each radiographic value (D1, D2, D3 and D4), the area under the receiver operating characteristic curve was calculated. The alpha angle and femoral tunnel length values were considered as gold standards. Results Analyses showed that the mean values for D1, D2, D3 and D4 were 13.20 ± 0.54, 39.44 ± 0.31, 1.65 ± 0.15 and 42.66 ± 0.47 mm, respectively. The mean angle was found to be 38.6 ± 0.3°, and the mean femoral tunnel length was 38.6 ± 0.2 mm. Age was significantly related to D2 and the diameter of the femur in AP X‐ray, while body mass index had a significant relation with D3 (p

ABSTRACT Objective This study assessed the effect of the anodization of titanium abutments on the color parameters and color difference of lithium disilicate (LDS) all‐ceramic crowns. Materials and Methods In this study, 19 straight abutments were divided into two groups: anodized (n = 9) and non‐anodized control (n = 9), with one hybrid zirconia abutment as a reference. Anodization was achieved by applying 63 V energy using seven 9 V flat batteries in series, with an electrolyte solution comprising 1 g trisodium phosphate in 250 mL distilled water for 5 s, resulting in a gold‐yellow color. Abutments were then scanned, and full‐contour monolithic IPS e.max maxillary central incisor crowns were fabricated with 2 mm thickness and glazed. Reflectance was measured using a spectroradiometer, and color coordinates (L*, a*, b*, h*, and C*) were calculated using CS‐10W software. Color differences of the crowns in both groups were quantified using the CIEDE2000 (ΔE00) color difference formula and analyzed by t‐test (α = 0.05) compared to the standard sample. Results The L*, a*, b*, and c* parameters in anodized abutments were significantly higher than those in non‐anodized abutments, while the h* parameter in anodized abutments was significantly lower than that in non‐anodized abutments (p

Abstract Background and Aims Paravalvular leak (PVL) is a serious complication of prosthetic valve replacement. Both surgical and percutaneous closure techniques are used for PVL closure, but optimal strategies and comparative outcomes are uncertain. This study aimed to compare the efficacy and safety of percutaneous versus surgical PVL closure by analyzing changes in leak severity, functional status, echocardiographic parameters, and clinical outcomes. Methods A total of 72 patients were included in this retrospective cross‐sectional single‐center study comparing percutaneous (n = 25) and surgical (n = 47) PVL closure from 2015 to 2022. Demographics, medical history, echocardiograms, laboratory data, complications, and mortality data were extracted from the records. Changes in leak severity, NYHA class, echocardiographic parameters, and clinical outcomes were compared between the percutaneous and surgical groups. Results Both percutaneous and surgical PVL closure significantly reduced leak severity and improved NYHA class (both p

Abstract Objectives The objective of this study was to investigate the relationship of the depression level with temporomandibular disorders (TMDs) in young conscripts as a population with chronic stresses. Material and Methods A total number of 144 male conscripts with chronic stress and different levels of depression were assigned to four groups according to the Beck's Depression Inventory (BDI). The control group consisted of age‐matched male conscripts without chronic stress. The diagnosis of TMD was made according to the Diagnostic Criteria for Temporomandibular Disorders (DC/TMD). Data were analyzed using Mann–Whitney and chi‐square tests. Results The participants with severe depression were significantly more susceptible to have TMD (p = .001) followed by the moderate depression, borderline clinical depression, mild mood disturbance, and control groups. The TMD diagnoses were more prevalent within depression groups compared with the control population (p = .01). Conclusions The level of depression is directly associated with the presence of TMD in young men with chronic stress.

Abstract Background and aims The nonmotor symptoms (NMS) of Parkinson's disease (PD) and their potential role in early diagnosis are recent debates. Herein, we aimed to investigate the association between depression and NMS of PD including sleep disorders, hyposexuality, hyposmia, constipation, and orthostatic hypotension. Methods A total of 93 PD patients with depression and 67 PD patients without depression were included in the study, and NMS were compared between the two groups. Furthermore, the possible associations between depression severity measured by Beck Depression Inventory (BDI) and NMS were investigated using linear regression or binary logistic regression models controlled for possible confounders. Eventually, we performed a subgroup analysis in each mild, moderate, and severe depression group. Results Orthostatic hypotension, constipation, and hyposexuality showed a significant difference between PD patients with and without depression (p