10 results on '"Marja Kik"'
Search Results
2. Association of red-mark syndrome with a Rickettsia-like organism and its connection with strawberry disease in the USA
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Sonja J. Lloyd, Matthijs Metselaar, Marja Kik, Alexandra Adams, Smith Patrick R, Kimberly Thompson, Scott E. LaPatra, Douglas R. Call, and Remi L. Gratacap
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Antiserum ,biology ,medicine.drug_class ,Veterinary (miscellaneous) ,Aquatic Science ,Monoclonal antibody ,Molecular biology ,law.invention ,Microbiology ,law ,Polyclonal antibodies ,Piscirickettsia salmonis ,biology.protein ,medicine ,Immunohistochemistry ,Antibody ,Ribosomal DNA ,Polymerase chain reaction - Abstract
Red-mark syndrome (RMS), a disease seen mostly in rainbow trout, Oncorhynchus mykiss, is of unknown aetiology. The research presented here indicates the presence of an intracellular bacterium in RMS-affected fish. A positive reaction was observed using immunohistochemistry (IHC) with skin lesions, liver, kidney and spleen of affected fish sampled from several locations within the United Kingdom using two different polyclonal antisera raised against Piscirickettsia salmonis. The same reaction was also seen with a number of different anti-P. salmonis monoclonal antibodies (MAbs). A disease with similar clinical signs to RMS, referred to as strawberry disease (SD), has been reported in the USA. A Rickettsia-like organism (RLO) has recently been associated with SD based on analysis of 16S rDNA sequences. Using the same panel of anti-P. salmonis antibodies used to screen the RMS samples, similar staining was obtained in tissue of SD-affected fish by IHC. A polymerase chain reaction (PCR) using RLO-specific primers was also performed on RMS-affected fish from the United Kingdom, and the samples were positive for the RLO 16S rRNA sequence. These findings suggest that the same aetiological agent may be responsible for RMS in the United Kingdom and SD in the USA.
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- 2010
3. Biologic Behavior and Clinical Outcome of 25 Dogs with Canine Appendicular Chondrosarcoma Treated by Amputation: A Veterinary Society of Surgical Oncology Retrospective Study
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Stephen J. Withrow, Michael S. Kent, James P. Farese, Rod C. Straw, Suzanne Shelly Waltman, Jolle Kirpensteijn, Bernard Séguin, Julius M. Liptak, Yang Jiang, Myron Chang, Nicholas J. Bacon, and Marja Kik
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medicine.medical_specialty ,animal structures ,General Veterinary ,business.industry ,Proportional hazards model ,medicine.medical_treatment ,Retrospective cohort study ,musculoskeletal system ,medicine.disease ,Surgery ,Amputation ,Surgical oncology ,embryonic structures ,medicine ,Histopathology ,Clinical significance ,Chondrosarcoma ,business ,Survival analysis - Abstract
Objective— To characterize biologic behavior, clinical outcome, and effect of histologic grade on prognosis for dogs with appendicular chondrosarcoma treated by amputation alone. Study Design— Case series. Animals— Dogs (n=25) with appendicular chondrosarcoma. Methods— Medical records were searched to identify dogs with appendicular chondrosarcoma treated by limb amputation alone. Information recorded included signalment, anatomic location, radiographic appearance, and development of metastasis. Histopathologic diagnosis was confirmed and graded (1, 2, or 3). Survival curves were generated by the Kaplan–Meier method and the association between covariates (gender, age, weight, and tumor grade) and survival were evaluated using the univariate proportional hazards model. Results— Histopathology slides were available for 25 dogs. Rates of pulmonary metastasis were as follows: grade 1–0%, grade 2–31%, and grade 3–50%. Overall median survival time (MST) was 979 days. Age, weight, and sex were not significantly associated with survival (P=.16; .33; and .31, respectively). Survival was significantly associated with tumor grade (P=.008), with dogs with tumor grade of 1, 2, and 3 having MSTs of 6, 2.7, and 0.9 years, respectively. Conclusion— Canine appendicular chondrosarcoma can be treated effectively with amputation alone. Low to intermediate grade chondrosarcoma has a good prognosis, whereas high-grade tumors appear to behave aggressively. Clinical Relevance— The overall prognosis for appendicular chondrosarcoma is better than that of appendicular osteosarcoma treated by amputation alone or in combination with chemotherapy.
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- 2009
4. TP53 Gene Mutations in Canine Osteosarcoma
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Erik Teske, Gerard R. Rutteman, Jolle Kirpensteijn, and Marja Kik
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Male ,Pathology ,medicine.medical_specialty ,040301 veterinary sciences ,Bone Neoplasms ,Kaplan-Meier Estimate ,Gene mutation ,medicine.disease_cause ,Polymerase Chain Reaction ,Canine Osteosarcoma ,Disease-Free Survival ,0403 veterinary science ,03 medical and health sciences ,Dogs ,0302 clinical medicine ,Polymorphism (computer science) ,Gene expression ,Biomarkers, Tumor ,medicine ,Animals ,Point Mutation ,Dog Diseases ,Gene ,Polymorphism, Single-Stranded Conformational ,Neoplasm Staging ,Osteosarcoma ,Mutation ,General Veterinary ,business.industry ,Point mutation ,04 agricultural and veterinary sciences ,Alkaline Phosphatase ,Genes, p53 ,medicine.disease ,Primary tumor ,3. Good health ,Treatment Outcome ,030220 oncology & carcinogenesis ,Cancer research ,Female ,business - Abstract
Objective— To investigate mutations of the TP53 gene in canine osteosarcoma (OS). Study Design— Clinical historic cohort study. Animals— Client-owned dogs. Methods— OS (n=59) were screened for mutations of the complete TP53 gene using polymerase chain reaction and the mutation was analyzed by single-strand conformational polymorphism. Clinical outcome of dogs with TP53-mutated OS were compared with dogs with OS without a mutation after complete surgical excision of the primary tumor. Results— TP53 gene mutations were observed in 24 of 59 (40.7%) OS; 3 mutated OS had 2 mutations. The alterations consisted mainly of point mutations (74%). Dogs with mutated OS had a significantly shorter survival time (ST) after surgery than dogs with normal tumor TP53 gene expression (P=.03). Other significant prognosticators for ST and disease-free interval included elevated serum alkaline phosphatase (P
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- 2008
5. Cartilage-free areas in the elbow joint of young golden retrievers
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Marja Kik, W.A. Weijs, and C.F. Wolschrijn
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Cartilage, Articular ,musculoskeletal diseases ,Elbow ,Ulna ,Dogs ,Forelimb ,medicine ,Animals ,business.industry ,Hyaline cartilage ,Cartilage ,Age Factors ,Anatomy ,Humerus ,Agricultural and Biological Sciences (miscellaneous) ,Radius ,medicine.anatomical_structure ,Synovial Cell ,Trochlear notch ,Fibrocartilage ,Joints ,Joint Diseases ,Synovial membrane ,business - Abstract
The present study describes cartilage-free areas on the ulnar trochlear notch and the humeral condyle of eight very young golden retrievers with otherwise healthy elbow joints. Remarkably, the youngest dog with full-thickness cartilage-free areas was only 8 weeks old. The younger dogs showed no macroscopic abnormalities on the locations that were affected in the older dogs. Two kinds of cartilage modifications were found. Cartilage-free areas at the edges of the articular cartilage layer were present on the humeral capitulum and on two locations of the ulna, (the medial and lateral at the base of the anconeal process, and the trochlear notch near the lateral coronoid process, which was fractured in two cases). Histological examination showed that these cartilage-free areas were filled with dense supportive tissue. Synovial cells covered this tissue as well as the surrounding hyaline cartilage. The synovial membrane covering the areas was macroscopically enlarged, but histological examination revealed no signs of inflammation. The second type of modification consisted of discoloration of the articular surface at the humeral trochlea. Histological examination revealed that in this area the articular surface was composed of fibrocartilage instead of hyaline cartilage. Apparently, there are locations within the elbow joint in which articular cartilage is not necessary for normal joint functioning. The presence of fibrocartilage on the articular surface of the humeral condyle is a surprising finding, for which no explanation has yet been found.
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- 2003
6. Thyroid adenocarcinoma in two parrots
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Yvonne R.A. van Zeeland, Marja Kik, Hans Cornelissen, Richard Ducatelle, and Christina Doukaki
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endocrine system ,Pathology ,medicine.medical_specialty ,endocrine system diseases ,General Veterinary ,biology ,Respiratory distress ,business.industry ,Grey parrot ,Thyroid ,biology.organism_classification ,Thyroid Adenocarcinoma ,medicine.anatomical_structure ,Melena ,Medicine ,medicine.symptom ,business - Abstract
A Grey parrot and a Yellow-crowned Amazon were presented with severe respiratory distress and weight loss, and collapse and melena, respectively. Despite supportive therapy, both parrots quickly succumbed and died, with no ante-mortem diagnosis made. Postmortem examinations yielded a diagnosis of a follicular and papillary form of thyroid adenocarcinoma. Although thyroid neoplasia has been reported in birds, these two cases are the first described thyroid neoplasia in these parrot species and the first reported papillary form of thyroid adenocarcinoma in birds.
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- 2014
7. Clinical diagnosis of aneurysm of the right coronary artery in a white cockatoo ( Cacatua alba )
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Mariette Vink-Nooteboom, J.T. Lumeij, W.T.C. Wolvekamp, Marja Kik, and Nico J. Schoemaker
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Aortic valve ,medicine.medical_specialty ,Cacatua ,Arteriosclerosis ,Pericardial effusion ,Psittaciformes ,Diagnosis, Differential ,Aneurysm ,Internal medicine ,medicine.artery ,Ascites ,medicine ,Animals ,Angiocardiography ,Small Animals ,biology ,medicine.diagnostic_test ,Bird Diseases ,business.industry ,Angiography ,Coronary Aneurysm ,biology.organism_classification ,medicine.disease ,Coronary Vessels ,medicine.anatomical_structure ,Right coronary artery ,cardiovascular system ,Cardiology ,Heart murmur ,medicine.symptom ,business - Abstract
A 16-year-old male white cockatoo was presented with lethargy and a decreased appetite. Auscultation between the second and third sternal rib revealed a heart murmur, which was confirmed by electrocardiographic and phonocardiographic examination to be systolic, with a shift of the heart axis to -152 degrees. Radiographs showed lack of detail in the cranial part of the abdominal coelom, indicative of ascites and an enlarged cardiac shadow, while ultrasonographic examination revealed pericardial effusion and fluid accumulation in the cavitas peritonealis hepatica. An extra fluid-filled cavity was found at the atrioventricular junction in the right cardiac wall and colour Doppler examination demonstrated a turbulent jetstream of blood into the cavity, originating directly above the aortic valve. Non-selective angiocardiography confirmed the ultrasonographic observations. Findings were indicative of an aneurysm of the a. coronaria dextra (right coronary artery). This was confirmed by necropsy which revealed atherosclerosis to be the underlying cause.
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- 1998
8. Effects ofPhaseolus vulgarisisolectins on the structure and function of the small intestinal mucosa in the self-emptying blind-pouch model in piglets
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D. Kleef, Marja Kik, and A. Muysenberg
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Small intestinal mucosa ,Food Animals ,biology ,Chemistry ,Isolectins ,Animal Science and Zoology ,Phaseolus ,biology.organism_classification ,Molecular biology ,Structure and function - Abstract
Zusammenfassung Der Einflus von Phaseolus vulgaris-Isolektinen auf die Struktur und Funktion der Mucosa des Dunndarms beim Ferkel, beobachtet mit Hilfe einer sicb selbst entleerenden blinden Darmschleife Spezifisch pathogen freie Ferkel wurden operativ mit einer sich selbst entleerenden blinden Darmschleife im Jejunum versehen. Am Blindende der Darmschleife wurde eine Kanule eingesetzt. Durch die Kanule wurden Biopsien der jejunalen Mucosa entnommen. Die Biopsien wurden wahrend der Operation und dann 10 Tage post operationem in regelmasigen Abstanden 18 Tage vor und nach der Verabreichung einer einmaligen Dosis von Phaseolus vulgaris-Isolektin- E 4 und-L 4 durch die Kanule entnommen. Dann wurde die Morphologie der Mucosabiopsien licht- und elektronenmikroskopisch untersucht. Auserdem wurde die Aktivitat der Sucrase-Isomaltase bestimmt. In den ersten 18 Tagen nach der Operation konnten keine Veranderungen hinsichtlich Morphologie und Funktion der jejunalen Mucosa festgestellt werden. Nach der Verabreichung von Phaseolus vulgaris-Isolektin- E 4 fiel das Villus: Krypten-Verhaltnis ab. Die Glycocalyx war verdickt und die Microvilli zeigten sich verkurzt und waren unregelmasig angeordnet. Die Vesikel-Cluster waren von den Microvilli abgerissen. 48 h nach der Verabreichung des Isolektins waren diese Effekte noch sichtbar. Die Aktivitat der Sucrase-Isomaltase zeigte grose Schwankungen. Die Effekte nach der Verabreichung von Phaseolus vulgaris-Isolektin-L 4 waren ahnlich denen von -E 4 aber nicht so ausgepragt.
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- 1995
9. Actin cytoskeletal lesions in differentiated human colon carcinoma Caco-2 cells after exposure to soybean agglutinin
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Marjan Draaijer, Jaap E. van Dijk, J. M. V. M. Mouwen, J. F. J. G. Koninkx, Marja Kik, and Henno Hendriks
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food and beverages ,macromolecular substances ,Cell Biology ,General Medicine ,Biology ,Actin cytoskeleton ,Molecular biology ,Caco-2 ,Fluorescence microscope ,Soybean agglutinin ,Cytoskeleton ,Incubation ,Actin ,Intracellular - Abstract
We have investigated the effects of soybean agglutinin on the cytoskeletal element actin in differentiated Caco-2 cells. The actin cytoskeleton of the cells was visualized by fluorescence microscopy using 7-nitrobenz-2-oxa-1, 3-diazole phallacidin as a specific marker for F-actin. Compared with control Caco-2 cells no changes in the fluorescence pattern were observed after incubation with soybean agglutinin. However, using the deoxyribonuclease-I inhibition assay a dose-related response was noted in the increase of intracellular G-actin after a 2-hour incubation period with soybean agglutinin. Already after exposure for 15 min to soybean agglutinin a decrease in intracellular F-actin was demonstrable. This apparent depolymerization could be prevented by incubating the Caco-2 cells with soybean agglutinin and the appropriate monosaccharide simultaneously. The increase in the amount of G-actin appeared to be correlated with a shortening of microvilli on the Caco-2 cells.
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- 1989
10. A small intestinal mucosal biopsy technique in cannulated pigs
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P. van Leeuwen, J. M. V. M. Mouwen, J. E. Van Dijk, Marja Kik, and Instituut voor Landbouwkundig Onderzoek en Biochemische Produkten TNO
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Pathology ,medicine.medical_specialty ,Food Animals ,business.industry ,Medicine ,Animal Science and Zoology ,Mucosal Biopsy ,business ,Nutrition - Abstract
Zusammenfassung Eine Biopsietechnik zur Gewinnung von Dunndarm-Schleimhaut bei fistulierten Schweinen Um in vivo fur Versuchszwecke bei Schweinen Dunndarm-Schleimhaut zu gewinnen, wurde eine Biopsie-Technik bei fistulierten Schweinen mittels Entnahme von Dunndarm-Schleimhaut uber eine Jejunalfistel entwickelt und vorgestellt.
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- 1988
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