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Your search keyword '"Luo, Xiaolong"' showing total 25 results
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8. Cover Image, Volume 114, Number 1, January 2017

Author

Wu, Hsuan-Chen, primary, Quan, David N., additional, Tsao, Chen-Yu, additional, Liu, Yi, additional, Terrell, Jessica L., additional, Luo, Xiaolong, additional, Yang, Jen-Chang, additional, Payne, Gregory F., additional, and Bentley, William E., additional

Published
2016
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11. Integrated biofabrication for electro-addressed in-film bioprocessing

Author

Terrell, Jessica L., primary, Gordonov, Tanya, additional, Cheng, Yi, additional, Wu, Hsuan-Chen, additional, Sampey, Darryl, additional, Luo, Xiaolong, additional, Tsao, Chen-Yu, additional, Ghodssi, Reza, additional, Rubloff, Gary W., additional, Payne, Gregory F., additional, and Bentley, William E., additional

Published
2012
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24. Conferring biological activity to native spider silk: A biofunctionalized protein-based microfiber.

Author

Wu HC, Quan DN, Tsao CY, Liu Y, Terrell JL, Luo X, Yang JC, Payne GF, and Bentley WE

Subjects
Animals, Antibodies chemistry, Antibodies metabolism, Biocompatible Materials chemistry, Biocompatible Materials metabolism, Biotechnology, Cell Line, Tumor, Cell Separation methods, Female, Genetic Engineering, Humans, Spiders, Transglutaminases chemistry, Transglutaminases genetics, Transglutaminases metabolism, Recombinant Fusion Proteins chemistry, Recombinant Fusion Proteins genetics, Recombinant Fusion Proteins metabolism, Silk chemistry, Silk genetics, Silk metabolism
Abstract

Spider silk is an extraordinary material with physical properties comparable to the best scaffolding/structural materials, and as a fiber it can be manipulated with ease into a variety of configurations. Our work here demonstrates that natural spider silk fibers can also be used to organize biological components on and in devices through rapid and simple means. Micron scale spider silk fibers (5-10 μm in diameter) were surface modified with a variety of biological entities engineered with pentaglutamine tags via microbial transglutaminase (mTG). Enzymes, enzyme pathways, antibodies, and fluorescent proteins were all assembled onto spider silk fibers using this biomolecular engineering/biofabrication process. Additionally, arrangement of biofunctionalized fiber should in of itself generate a secondary level of biomolecular organization. Toward this end, as proofs of principle, spatially defined arrangement of biofunctionalized spider silk fiber was shown to generate effects specific to silk position in two cases. In one instance, arrangement perpendicular to a flow produced selective head and neck carcinoma cell capture on silk with antibodies complexed to conjugated protein G. In a second scenario, asymmetric bacterial chemotaxis arose from asymmetric conjugation of enzymes to arranged silk. Overall, the biofabrication processes used here were rapid, required no complex chemistries, were biologically benign, and also the resulting engineered silk microfibers were flexible, readily manipulated and functionally active. Deployed here in microfluidic environments, biofunctional spider silk fiber provides a means to convey complex biological functions over a range of scales, further extending its potential as a biomaterial in biotechnological settings. Biotechnol. Bioeng. 2017;114: 83-95. © 2016 Wiley Periodicals, Inc., (© 2016 Wiley Periodicals, Inc.)

Published
2017
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25. Protein assembly onto patterned microfabricated devices through enzymatic activation of fusion pro-tag.

Author

Lewandowski AT, Yi H, Luo X, Payne GF, Ghodssi R, Rubloff GW, and Bentley WE

Subjects
Binding Sites, Biosensing Techniques methods, Electrochemistry methods, Enzyme Activation, Equipment Design, Equipment Failure Analysis, Protein Binding, Biosensing Techniques instrumentation, Electrochemistry instrumentation, Monophenol Monooxygenase chemistry, Recombinant Fusion Proteins chemistry
Abstract

We report a versatile approach for covalent surface-assembly of proteins onto selected electrode patterns of pre-fabricated devices. Our approach is based on electro-assembly of the aminopolysaccharide chitosan scaffold as a stable thin film onto patterned conductive surfaces of the device, which is followed by covalent assembly of the target protein onto the scaffold surface upon enzymatic activation of the protein's "pro-tag." For our demonstration, the model target protein is green fluorescent protein (GFP) genetically fused with a pentatyrosine pro-tag at its C-terminus, which assembles onto both two-dimensional chips and within fully packaged microfluidic devices in situ and under flow. Our surface-assembly approach enables spatial selectivity and orientational control under mild experimental conditions. We believe that our integrated approach harnessing genetic manipulation, in situ enzymatic activation, and electro-assembly makes it advantageous for a wide variety of bioMEMS and biosensing applications that require facile "biofunctionalization" of microfabricated devices., ((c) 2007 Wiley Periodicals, Inc.)

Published
2008
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