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Your search keyword '"Lin, Pao-Chun"' showing total 17 results
Start Over Author "Lin, Pao-Chun" Publisher wiley
17 results on '"Lin, Pao-Chun"'

3. Seismic performance evaluation of single damped‐outrigger system incorporating buckling‐restrained braces

Author

LIN, Pao-Chun, Lin, Pao-Chun, Takeuchi, Toru, and Matsui, Ryota

Subjects
021110 strategic, defence & security studies, Buckling, Computer science, business.industry, Outrigger, 0211 other engineering and technologies, Earth and Planetary Sciences (miscellaneous), 020101 civil engineering, 02 engineering and technology, Structural engineering, Geotechnical Engineering and Engineering Geology, business, 0201 civil engineering
Published
2018

14. Seismic design and hybrid tests of a full-scale three-story buckling-restrained braced frame using welded end connections and thin profile

Author

Lin, Pao-Chun, primary, Tsai, Keh-Chyuan, additional, Wang, Kung-Juin, additional, Yu, Yi-Jer, additional, Wei, Chih-Yu, additional, Wu, An-Chien, additional, Tsai, Ching-Yi, additional, Lin, Chih-Han, additional, Chen, Jia-Chian, additional, Schellenberg, Andreas H., additional, Mahin, Stephen A., additional, and Roeder, Charles W., additional

Published
2011
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17. TGF-beta1 regulation in hepatocyte-NIH3T3 co-culture is important for the enhanced hepatocyte function in 3D microenvironment.

Author

Chia SM, Lin PC, and Yu H

Subjects
Animals, Coculture Techniques, Culture Media, Conditioned, Cytochrome P-450 CYP1A1 analysis, Cytochrome P-450 CYP1A1 metabolism, Enzyme-Linked Immunosorbent Assay, Extracellular Matrix, Fibroblasts cytology, Hepatocytes cytology, Hepatocytes metabolism, Mice, NIH 3T3 Cells, Reverse Transcriptase Polymerase Chain Reaction, Spheroids, Cellular metabolism, Time Factors, Transforming Growth Factor beta metabolism, Transforming Growth Factor beta1, Up-Regulation, Biotechnology methods, Fibroblasts metabolism, Hepatocytes drug effects, Transforming Growth Factor beta pharmacology
Abstract

Co-culture of hepatocytes or hepatocyte spheroids with the supporting NIH3T3 in a 3D microcapsule formed with a hybrid natural/synthetic matrix has led to enhanced hepatocyte functions. We investigated the mechanism of the functional enhancement in co-culture with respect to the contributions of soluble factors and direct cell-cell interactions. The conditioned media from the co-culture induced higher P450 cytochrome oxidase activity (indicated by EROD assay) in the microencapsulated hepatocytes than the conditioned media from the NIH3T3- or the hepatocytes-alone controls. Conditioned media from physically separated co-culture of hepatocytes-NIH3T3 by a membrane insert reduced the functional enhancement. Among the known stimulators of hepatocyte functions, TGF(beta)1 is primarily responsible for the stimulation of hepatocyte functions in this 3D co-culture since the removal of TGF(beta)1 by antibody depletion eliminated the functional enhancement and the reconstitution of TGF(beta)1 restored the functional enhancement. Activation of latent TGF(beta)1 in an extracellular environment were upregulated in co-culture with no observable increase in the TGF(beta)1 expression at transcriptional and translational levels. Our data led to an improved understanding of how co-culture enhances hepatocyte functions in vitro and pave the way for further innovations in liver tissue engineering, drug metabolism studies, and other applications that require functional hepatocytes cultured in vitro., (2005 Wiley Periodicals, Inc.)

Published
2005
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