Search

Your search keyword '"Lee, Bok Luel"' showing total 21 results
Start Over Author "Lee, Bok Luel" Publisher wiley
21 results on '"Lee, Bok Luel"'

17. Molecular cloning of cDNA for pro-phenol-oxidase-activating factor I, a serine protease is induced by lipopolysaccharide or 1,3-beta-glucan in coleopteran insect, Holotrichia diomphalia larvae

Author

Lee, So Young, primary, Cho, Mi Young, additional, Hyun, Ji Hoon, additional, Lee, Kwang Moon, additional, Homma, Ko-Ichi, additional, Natori, Shunji, additional, Kawabata, Shun-Ichiro, additional, Iwanaga, Sadaaki, additional, and Lee, Bok Luel, additional

Published
1998
Full Text
View/download PDF

20. Symbiotic factors in Burkholderia essential for establishing an association with the bean bug, Riptortus pedestris.

Author

Kim JK and Lee BL

Subjects
Animals, Burkholderia genetics, Gastrointestinal Tract microbiology, Heteroptera physiology, Larva microbiology, Burkholderia physiology, Heteroptera microbiology, Symbiosis physiology
Abstract

Symbiotic bacteria are common in insects and intimately affect the various aspects of insect host biology. In a number of insect symbiosis models, it has been possible to elucidate the effects of the symbiont on host biology, whereas there is a limited understanding of the impact of the association on the bacterial symbiont, mainly due to the difficulty of cultivating insect symbionts in vitro. Furthermore, the molecular features that determine the establishment and persistence of the symbionts in their host (i.e., symbiotic factors) have remained elusive. However, the recently established model, the bean bug Riptortus pedestris, provides a good opportunity to study bacterial symbiotic factors at a molecular level through their cultivable symbionts. Bean bugs acquire genus Burkholderia cells from the environment and harbor them as gut symbionts in the specialized posterior midgut. The genome of the Burkholderia symbiont was sequenced, and the genomic information was used to generate genetically manipulated Burkholderia symbiont strains. Using mutant symbionts, we identified several novel symbiotic factors necessary for establishing a successful association with the host gut. In this review, these symbiotic factors are classified into three categories based on the colonization dynamics of the mutant symbiont strains: initiation, accommodation, and persistence factors. In addition, the molecular characteristics of the symbiotic factors are described. These newly identified symbiotic factors and on-going studies of the Riptortus-Burkholderia symbiosis are expected to contribute to the understanding of the molecular cross-talk between insects and bacterial symbionts that are of ecological and evolutionary importance., (© 2014 Wiley Periodicals, Inc.)

Published
2015
Full Text
View/download PDF

21. Depletion of autophagy-related genes ATG3 and ATG5 in Tenebrio molitor leads to decreased survivability against an intracellular pathogen, Listeria monocytogenes.

Author

Tindwa H, Jo YH, Patnaik BB, Noh MY, Kim DH, Kim I, Han YS, Lee YS, Lee BL, and Kim NJ

Subjects
Animals, DNA, Complementary genetics, Immunity, Innate, Larva immunology, Larva microbiology, Listeria monocytogenes immunology, Listeria monocytogenes physiology, RNA Interference, RNA, Double-Stranded, RNA, Messenger genetics, Sequence Analysis, DNA, Sequence Analysis, Protein, Autophagy genetics, Tenebrio genetics, Tenebrio immunology, Tenebrio microbiology
Abstract

Macroautophagy (autophagy) is an evolutionarily conserved catabolic process involved in physiological and developmental processes including cell survival, death, and innate immunity. Homologues of most of 36 originally discovered autophagy-related (ATG) genes in yeast have been characterized in higher eukaryotes including insects. In this study, the homologues of ATG3 (TmATG3) and ATG5 (TmATG5) were isolated from the coleopteran beetle, Tenebrio molitor by expressed sequence tag and RNAseq approaches. The cDNA of TmATG3 and TmATG5 comprise open-reading frame sizes of 963 and 792 bp encoding polypeptides of 320 and 263 amino acid residues, respectively. TmATG3 and TmATG5 mRNA are expressed in all developmental stages, and mainly in fat body and hemocytes of larvae. TmATG3 and TmATG5 showed an overall sequence identity of 58-95% to other insect Atg proteins. There exist clear one-to-one orthologs of TmATG3 and TmATG5 in Tribolium and that they clustered together in the gene tree. Depletion of TmATG3 and TmATG5 by RNA interference led to a significant reduction in survival ability of T. molitor larvae against an intracellular pathogen, Listeria monocytogenes. Six days post-Listeria challenge, the survival rate in the dsEGFP-injected (where EGFP is enhanced green fluorescent protein) control larvae was significantly higher (55%) compared to 4 and 3% for TmATG3 and TmATG5 double-stranded RNA injected larvae, respectively. These data suggested that TmATG3 and TmATG5 may play putative role in mediating autophagy-based clearance of Listeria in T. molitor model., (© 2014 Wiley Periodicals, Inc.)

Published
2015
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results