401 results on '"King, C"'
Search Results
2. Unconventional GPCR‐PKA Signaling in the Hedgehog Pathway
- Author
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Myers, Benjamin, primary, Happ, John, additional, Arveseth, Corvin, additional, Bruystens, Jessica, additional, Bertinetti, Daniela, additional, Nelson, Isaac, additional, Olivieri, Cristina, additional, Hedeen, Danielle, additional, Zhu, Ju‐Fen, additional, Capener, Jacob, additional, Broeckel, Jan, additional, Vu, Lily, additional, King, C. C., additional, Ruiz‐Perez, Victor, additional, Veglia, Gianluigi, additional, Herberg, Friedrich, additional, and Taylor, Susan, additional
- Published
- 2022
- Full Text
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3. MicroRNA regulatory networks associated with abnormal muscle repair in survivors of critical illness
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Walsh, CJ, Escudero King, C, Gupta, M, Plant, PJ, Herridge, MJ, Mathur, S, Hu, P, Correa, J, Ahmed, S, Bigot, A, dos Santos, CC, Batt, J, Walsh, CJ, Escudero King, C, Gupta, M, Plant, PJ, Herridge, MJ, Mathur, S, Hu, P, Correa, J, Ahmed, S, Bigot, A, dos Santos, CC, and Batt, J
- Abstract
BACKGROUND: Intensive care unit (ICU)-acquired weakness is characterized by muscle atrophy and impaired contractility that may persist after ICU discharge. Dysregulated muscle repair and regeneration gene co-expression networks are present in critical illness survivors with persistent muscle wasting and weakness. We aimed to identify microRNAs (miRs) regulating the gene networks and determine their role in the self-renewal of muscle in ICU survivors. METHODS: Muscle whole-transcriptome expression was assessed with microarrays in banked quadriceps biopsies obtained at 7 days and 6 months post-ICU discharge from critically ill patients (n = 15) in the RECOVER programme and healthy individuals (n = 8). We conducted an integrated miR-messenger RNA analysis to identify miR/gene pairs associated with muscle recovery post-critical illness and evaluated their impact on myoblast proliferation and differentiation in human AB1167 and murine C2C12 cell lines in vitro. Select target genes were validated with quantitative PCR. RESULTS: Twenty-two miRs were predicted to regulate the Day 7 post-ICU muscle transcriptome vs. controls. Thirty per cent of all differentially expressed genes shared a 3'UTR regulatory sequence for miR-424-3p/5p, which was 10-fold down-regulated in patients (P < 0.001) and correlated with quadriceps size (R = 0.86, P < 0.001), strength (R = 0.75, P = 0.007), and physical function (Functional Independence Measures motor subscore, R = 0.92, P < 0.001) suggesting its potential role as a master regulator of early recovery of muscle mass and strength following ICU discharge. Network analysis demonstrated enrichment for cellular respiration and muscle fate commitment/development related genes. At 6 months post-ICU discharge, a 14-miR expression signature, including miRs-490-3p and -744-5p, identified patients with muscle mass recovery vs. those with sustained atrophy. Constitutive overexpression of the novel miR-490-3p significantly inhibited AB1167 and C2C12 my
- Published
- 2022
4. Novel immediate/sustained‐release formulation of acetaminophen‐ibuprofen combination (Paxerol®) for severe nocturia associated with overactive bladder: A multi‐center, randomized, double blinded, placebo‐controlled, 4‐arm trial
- Author
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Johnathan A. Khusid, Yunzhu Zhao, Lei Xie, Igor Ryndin, King C. Lee, Frank J. Rauscher, Jeffrey P. Weiss, and Jed Kaminesky
- Subjects
Adult ,Male ,medicine.medical_specialty ,Urology ,030232 urology & nephrology ,Ibuprofen ,Placebo ,03 medical and health sciences ,0302 clinical medicine ,Double-Blind Method ,Original Clinical Article ,medicine ,Humans ,Nocturia ,Adverse effect ,Aged ,Acetaminophen ,Aged, 80 and over ,030219 obstetrics & reproductive medicine ,Urinary Bladder, Overactive ,business.industry ,clinical trial ,Middle Aged ,medicine.disease ,Clinical trial ,Drug Combinations ,Treatment Outcome ,investigational drug ,Tolerability ,Overactive bladder ,Delayed-Action Preparations ,Quality of Life ,Female ,Neurology (clinical) ,medicine.symptom ,Original Clinical Articles ,business ,prostagladin‐E2 inhibitors ,medicine.drug - Abstract
Aim To determine short-term efficacy and safety of Paxerol®, novel immediate:sustained (50%:50%) release tablets containing 325 mg acetaminophen and 150 mg ibuprofen per tablet. Methods One of three dose levels, corresponding to the amounts in 1, 2, and 3 tablets, of Paxerol and placebo were administered for 14 consecutive days to patients with severe nocturia (defined in this study as an average nocturnal voids [NV] ≥2.5) associated with overactive bladder (OAB). Changes in NV, as well as Nocturia Quality of Life (NQOL), duration of first uninterrupted sleep (DFUS), and total hours of nightly sleep (THNS) associated with treatment were assessed. Short-term safety/tolerability was assessed throughout the study and for at least 30 days post-treatment. Results Paxerol at all three doses reduced NV to a greater degree than placebo (average NV -1.1, -1.4, -1.3 voids for low, mid, and high doses, respectively, vs -0.3 void for placebo). NQOL and THNS were similar between baseline and treatment values in all four groups. There were also no between-group differences. Paxerol at high dose tended to (although not statistical significantly) increase DFUS to a greater degree than placebo (1.2 vs 0.4 h, P = 0.057). There were no treatment related adverse events in any of the four groups. Conclusions This study demonstrates short-term efficacy and short-term safety of Paxerol in patients with severe nocturia associated with OAB. The results warrant further investigation of the long-term efficacy and safety of Paxerol in larger patient populations.
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- 2018
5. Infantile-onset myoclonic developmental and epileptic encephalopathy: A new RARS2 phenotype
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de Valles-Ibanez, G, Hildebrand, MS, Bahlo, M, King, C, Coleman, M, Green, TE, Goldsmith, J, Davis, S, Gill, D, Mandelstam, S, Scheffer, IE, Sadleir, LG, de Valles-Ibanez, G, Hildebrand, MS, Bahlo, M, King, C, Coleman, M, Green, TE, Goldsmith, J, Davis, S, Gill, D, Mandelstam, S, Scheffer, IE, and Sadleir, LG
- Abstract
Recessive variants in RARS2, a nuclear gene encoding a mitochondrial protein, were initially reported in pontocerebellar hypoplasia. Subsequently, a recessive RARS2 early-infantile (<12 weeks) developmental and epileptic encephalopathy was described with hypoglycaemia and lactic acidosis. Here, we describe two unrelated patients with a novel RARS2 phenotype and reanalyse the published RARS2 epilepsy phenotypes and variants. Our novel cases had infantile-onset myoclonic developmental and epileptic encephalopathy, presenting with a progressive movement disorder from 9 months on a background of normal development. Development plateaued and regressed thereafter, with mild to profound impairment. Multiple drug-resistant generalized and focal seizures occurred with episodes of non-convulsive status epilepticus. Seizure types included absence, atonic, myoclonic, and focal seizures. Electroencephalograms showed diffuse slowing, multifocal, and generalised spike-wave activity, activated by sleep. Both patients had compound heterozygous RARS2 variants with likely impact on splicing and transcription. Remarkably, of the now 52 RARS2 variants reported in 54 patients, our reanalysis found that 44 (85%) have been shown to or are predicted to affect splicing or gene expression leading to protein truncation or nonsense-mediated decay. We expand the RARS2 phenotypic spectrum to include infantile encephalopathy and suggest this gene is enriched for pathogenic variants that disrupt splicing.
- Published
- 2021
6. General anaesthetic and airway management practice for obstetric surgery in England: a prospective, multicentre observational study
- Author
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Odor, P. M., Bampoe, S., Moonesinghe, S. R., Andrade, J., Pandit, J. J., Lucas, D. N., A’Court, A., Abdel-Gadir, D., Abdu, A., Abisogun, C., Aboud, Z., Abrams, J., Ackerman, A., Adamson, C., Addison, R., Adeyeye, A., Adler, R., Aduse-Poku, M., Adyanthaya, S., Ahmad, N., Ahmed, D., Ahmed, A., Akindele, B., Akindele, O., Akrimi, S., Al-Rawi, S., Ali, Y., Allam, J., Allana, A., Allen, K., Allen, O., Amaradasa, N., Amarasekara, L., Amoakwa-adu, F., Anandageetha, P., Anandakrishnan, S., Anandanadesan, R., Anderson, M., Apps, S., Aquilina, A., Arbane, G., Arch, A., Armstrong, S., Arya, R., Ashiru, G., Ashpole, K., Atkinson, C., Atkinson, F., Auer, E., Avery, B., Babio-Galan, M., Bader, H., Badham, G., Bagchi, S., Bailey, S., Baird, Y., Balaka, C., Baldwin, M., Balfour, P., Bali, S., Banks, S., Barclay, P., Barnes, L., Barnes, T., Barot, N., Barrett, S., Barrett, V., Barrett, K., Bates, L., Batte, K., Baytug, B., Behravesh, M., Bell, S., Benloch, R., Bentley, R., Berg, J., Berwick, C., Berwick, R., Bhadange, R., Bhattacharyya, S., Bielskute, E., Birch, S., Bird, S., Bird, Ruth, Birts, William, Black, Becky, Blagova, Tatyana, Blake, Holly, Blightman, Oliver, Blunden, Susara, Bolton, R., Borkett-Jones, C., Boselli, J., Bowen, M., Bowen, R., Bowyer, J., Boyle, H., Brar, Z., Bray, J., Brayshaw, S., Bressington, C., Brewer, A., Brice, N., Bridge, L., Briscoe, J., Brocklesby, S., Brown, H., Brown, S., Brunnen, D., Burijintichenna, K., Burnard, S., Burtt, A., Buswell, V., Bykar, H., Cairney, M., Calvert, C., Camarasa, L., Campbell, N., Campbell-Jones, F., Cantliffe, J., Carrol, W., Carvalho, J., Cashell, C., Cassie, S., Cassim, K., Chandler, M., Chapman, R., Charles, R., Chen, P., Cheyne, D., Chima, K., Chin, F., Chirvasuta, R., Shao Chong, M., Choudhury, S., Chowdhury, P., Christmas, T., Chughwani, S., Ciechanowicz, S., Clarey, E., Coe, R., Cohen, J., Coker, N., Collins, K., Collis, L., Comar, J., Conroy, M., Constantin, K., Corfe, J., Coulborn, E., Cowie, V., Crone, R., Cronin, J., Crooks, J., Crowther, N., Crowther, E., Cruz, C., Curtis, A., Curtis, S., Dabrowicz, A., Daines, N., Dalal, V., Dannatt, P., Das, D., Dash, J., Davidson, K., Davies, S., Davis, Y., Dawson, J., Dean, J., Dean, C., Denman, J., Desai, N., Dewan, P., Dimont, S., Donovan, C., Doraiswami, M., Doughty, K., Douglass, J., Dower, M., Downing, S., Duberry, W., Duckham, E., Dudgeon, L., Dukes, S., Dunn, L., Duraiswamy, V., Dwyer, A. O., Dyer, K., Eapen, S., Earl, M., Eason, S., Edwards, K., Edwards, Z., Egole, O., Ekpa, J., el-Amin, O., el-Boghdadly, K., Elbasir, O., Eldridge, J., Elgie, L., Ellington, M., Elliott, K., Elliott, J., Elmi, M., Elnoumeir, R., Emeakaraoha, E., Evans, M., Everett, M., Fabb, P., Farooq, H., Farrimond, R., Faulds, F., Fawcett, E., Feneley, A., Fernando, D., Ferns, J., Finlay, C., Fitzgerald, S., O’Flaherty, D., Fleet, M., Fletcher, L., Fludder, V., Follet, T., Forbes, J., Forth, M., Foster, G., Francis, J., Fraser, K., Friedman, L., Fruggeri, L., Fulton, L., Funnell, S., Gadre, A., Gandhi, A., Gardiner, H., Garner, Z., Garvey, G., Gately, T., George, R., Gillespie, S., Glover, S., Goddard, J., Goodman, B., Gopal, T., Graham, G., Green, D., Griffin, D., Griffith, J., Grigsby, S., Grindey, J., Griffiths, H., Groome, J., Grother, C., Grounds, G., Groves, A., Guha, A., Gunawardhana, A., Gupta, A., Gupta, R., Gutsell, J., Haddon, R., Hadi, D., Hadjipavlou, N., Hammerbeck, H., Hammon, L., Hammond, S., Hampanna, H., Hancock, H., Handapangoda, H., Haroon-Mowahed, Y., Harpham, D., Harris, G., Harrison, A., Harshan, D., Hartopp, A., Harty, E., Haslam, N., Hawkins, G., Hawkins, E., Hawksey, S., Hays, C., Hazelton, T., Heavyside, A., Hemeson, C., Henderson, K., Henry, O., Herbert, L., Higgins, N., Hilton, J., Hindmoor, C., Hitchcock, R., Hobbs, L., Homsy, M., Honeywell, C., Hoque, N., House, K., Howle, R., Tiller, A., Huniak, M., Hunte, J., Husain, T., Huson, C., Hussain, C., Hussain, T., Hussein, Z., Hyams, J., Hyde, E., Laverdino, M., Ignacka, A., Innes, E., Ioannidis, S., Iqbal, R., Ismail, F., Jackson, J., Jackson, M., Jackson, G., Jacobs, R., Jadhav, P., Jalaly, A., James, L., James, M., Jani, S., Jeganathan, C., Joannides, C., Johnson, R., Johnson, T., Johnston, C., Jones, R., Jones, T., Kadr, M., Kainth, R., Kane, J., Kanji, R., Kannanparambil, S., Kar, G., Kasianandan, T., Kaskos, H., Kavanagh, L., Kaye, R., Kelliher, L., Kelliot, S., Kelly, J., Kenyon, C., Kessack, L., Kestner, S., Khaku, M., Khaleeq, S., Khan, P., Khan, S., Kidwai, U., King, C., King, H., Kingston, E., Kok, W., Konig, R., Konstantinova, Z., Krishnan, P., Kua, J., Kuntumalla, K., Kursumovic, E., Kurzatkowski, K., Kuttambakam, H., Lane, K., Lane, S., Langton, A., Latif, H., Lau, N., Laxman, S., Laycock, H., Lee, R., Leonardi, S., Light, K., Lightfoot, H., Liu, S., Liyanage, S., Lowe, J., Lucas, N., Lungu, M., Lunn, M., Lynes, H., Machavarapu, K., Mackenzie, M., Magee, D., Major, J., Male, V., Malik, Z., Manso, K., Maquinana, M., Marciniak, K., Maronge, L., Marsh, C., Martella, C., Martin, N., Martins, N., Marway, J., Mason, L., Masood, N., Masters, J., Maton-Howarth, M., Mazzola, F., McAllister, T., McCarthy, R., McCormick, C., McCready, S., McDougall, S., Mcewan, L., McGarry, J., McKevitt, H., Mckinley, S., Mckskeane, A., McMaster, E., McMonagle, M., McNamara, H., McPhee, H., McRae, L., Mead, D., Meadows, E., Mehta, M., Meikle, J., Metodiev, Y., Michael, C., Millar, V., Miller, S., Miller, G., Milne, S., Miltsios, K., Misquita, L., Misquita, S., Mittal, M., Mohamed, M., Powell Monaghan, K., Monk, J., Monkhouse, A., Monks, D., Montague, L., Moon, A., Moran, J., Moreton, A., Morgan, E., Morgan, O., Morland, D., Morosan, M., Morris, K., Morris, A., Moser, C., Mount, M., Muir, C., Mupudzi, M., Murali, M., Murdoch, I., Murray, H., Murray, T., Murrell, K., Narasimha Murthy, G., Neeley, D., Nei, H., Neil, K., Nejim, T., Nel, M., Nicholson, A., Nicklin, A., Nolan, C., Nolan, T., Nurmi, E., O’Neill, B., Oakes, C., Oakes, N., Ochoa-Ferraro, M., Odeleye, N., Oliver, K., Oliver, M., Onslow, J., Onwochei, D., Oommen, T., Orr, T., Osagie, O., Osborn, H., Overend, J., Owston, H., Pack, E., Padhi, P., Palani, P., Pandey, R., Pandya, D., Panesar, N., Papageorgiou, C., Papanastasiou, G., Papoutsos, C., Pararajasingham, S., Parry, J., Patel, H., Patel, J., Patel, K., Patel, M., Patel, R., Patel, N., Pathak, S., Pearson, F., Peciulene, V., Peers, B., Peirce, B., Pepper, S., Perinpanayagam, J., Perry, H., Petrova, N., Phillips, T., Phillips, S., Phylactides, L., Pilkington, F., Plumb, J., Poimenidi, E., Sau Kuk Poon, A., Potter, T., Poultney, U., Powell, L., Prenter, A., Preston, K., Price, A., Pritchard, N., Pullen, J., Purohit, M., Quamina, C., Qureshi, J., Rajput, Z., Ramage, S., Ramanathan, T., Ranasinghe, U., Ranatunga, K., Rand, A., Randive, S., Rangarajan, D., Rao, C., Rao Pelluri, S., Ratnasingham, A., Razzaque, J., Reddy, A., Redington, K., Reel, E., Remeta, P., Ricco, F., Riccoboni, A., Rice, P., Rich, M., Richards, N., Riches, J., Ripoll, S., Roberts, F., Roberts, K., Robins, K., Robinson, S., Roche, S., Rojo, M., Carmela Romano, N., Rosser, H., Roughley, L., Routley, C., Rowley, C., Rudra, P., Russell, R., Ryan, C., Saad, C., Sadeghi, A., Salberg, A., Salota, V., Samuel, M., Samuels, R., Sanapala, S., Sanusi, S., Sarao, S., Sathyabhama, S., Saunders, Z., Sawarzynska-ryszka, B., Sceales, P., Schumacher, N., Schwartz, N., Sellers, C., Sellers, H., Sellick, J., Sen, S., Senaratne, D., Senbeto, S., Seneviratna, D., Setty, T., Shah, R., Shah, S., Shambly, J., Sharafudeen, S., Sharieff, I., Sharifi, L., Sharpe, L., Shaw, M., Sheldrake, I., Shinde, P., Shonfeld, A., Short, J., Siah, J., Sibug, S., Siddique, O., Siew, S., Simpson, M., Singleton, G., Sinha, K., Sinha, A., Sinnott, M., Sivadhas, H., Sivakumar, S., Sivarajan, B., Sivarajan, S., Skeoch, C., Slade, S., Slater, P., Smith, C., Smith, E., Smith, J., Smith, L., Smith, A., Smith, R., Smith, S., Smith, T., Smithers, H., Smolen, S., Smyth, C., Snel, T., Snipe, C., Soltanifar, S., Sonawane, N., Soundararaja, A., Spence, E., Spiliopoulos, M., Srivastava, C., Stacey, K., Stafford, H., Staines, N., Stead, R., Stevens, E., Stilwell, A., Stocks, G., Stokes, A., Stone, C., Straughan, B., Subbarathnam, V., Sudunagunta, S., Sultan, P., Suppiah, P., Surve, P., Sutherland, A., Swanton, R., Swarbrick, C., Swinson, A., Syrrakou, E., Tadbiri, S., Tamhane, P., Tamilselvan, P., Tan, A., Tanna, S., Tarft, H., Tarry, L., Taylor, I., Taylor, S., Tebbot, J., Theron, S., Thomas, M., Todd, S., Tolliday, H., Topham, C., Tovell, N., Traves, M., Trodd, D., Tufchi, A., Turley, K., Turnbull, M., Turnbull, C., Turner, O., Turner, W., Turney, S., Tyagi, E., Uncles, D., Unsworth, V., Vadnere, P., Varadan, R., Vasishta, V., Veal, A., Vedham, L., Venkaya, J., Verghese, M., Veronica, I., Vidanagamage, D., Vincent, R., Vyapury, V., Wain, H., Walbridge, F., Walker, E., Walsh, P., Walshe, E., Walters, M., Wan, Y., Wang, C., Wankhade, K., Waters, G., Watts, C., Webber, A., Wedgwood, T., Wee, M., Wellstead, S., White, A., Whitear, M., Whitefield, L., Wilkinson, S., Williams, L., Williams, R., Wilson, D., Wilson, S., Wimble, K., Winkley, E., Winslow, L., Winwright, P., Wloch, K., Wong, G., Wong, H., Man Wong, J., Wood, T., Wray, S., Wrench, I., Wu, J., Wynn, K., Yap, Y., Kuan Yeow, C., Young, E., Yusaf, A., Uz Zafar, S., Zeinali, D., Zhang, S., Zope, S., Zucco, L., Anwar, S., Blunt, N., Grover, V., Grailey, K., Gray, M., Highton, D., Hopkins, P., Kemp, H., Lo, Q., Martin, D., Morkane, C., O’Carroll, J., Oliver, C., Post, B., Visram, A., Wickham, A., General Paediatrics, Graduate School, Adult Psychiatry, and Pulmonology
- Subjects
medicine.medical_specialty ,medicine.medical_treatment ,Population ,03 medical and health sciences ,0302 clinical medicine ,030202 anesthesiology ,medicine ,Caesarean section ,General anaesthesia ,general anaesthesia ,030212 general & internal medicine ,Rocuronium ,General anaesthetic ,education ,Neuromuscular Blockade ,education.field_of_study ,airway management ,obstetrics ,business.industry ,Tracheal intubation ,Surgery ,Anesthesiology and Pain Medicine ,caesarean section ,Airway management ,business ,medicine.drug - Abstract
There are no current descriptions of general anaesthesia characteristics for obstetric surgery, despite recent changes to patient baseline characteristics and airway management guidelines. This analysis of data from the direct reporting of awareness in maternity patients' (DREAMY) study of accidental awareness during obstetric anaesthesia aimed to describe practice for obstetric general anaesthesia in England and compare with earlier surveys and best-practice recommendations. Consenting patients who received general anaesthesia for obstetric surgery in 72 hospitals from May 2017 to August 2018 were included. Baseline characteristics, airway management, anaesthetic techniques and major complications were collected. Descriptive analysis, binary logistic regression modelling and comparisons with earlier data were conducted. Data were collected from 3117 procedures, including 2554 (81.9%) caesarean deliveries. Thiopental was the induction drug in 1649 (52.9%) patients, compared with propofol in 1419 (45.5%). Suxamethonium was the neuromuscular blocking drug for tracheal intubation in 2631 (86.1%), compared with rocuronium in 367 (11.8%). Difficult tracheal intubation was reported in 1 in 19 (95%CI 1 in 16-22) and failed intubation in 1 in 312 (95%CI 1 in 169-667). Obese patients were over-represented compared with national baselines and associated with difficult, but not failed intubation. There was more evidence of change in practice for induction drugs (increased use of propofol) than neuromuscular blocking drugs (suxamethonium remains the most popular). There was evidence of improvement in practice, with increased monitoring and reversal of neuromuscular blockade (although this remains suboptimal). Despite a high risk of difficult intubation in this population, videolaryngoscopy was rarely used (1.9%).
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- 2020
7. A mixed-methods evaluation of stakeholder perspectives on pediatric pneumonia in Nigeria-priorities, challenges, and champions
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King, C, Iuliano, A, Burgess, RA, Agwai, I, Ahmar, S, Aranda, Z, Bahiru, S, Bakare, AA, Colbourn, T, Shittu, F, Graham, H, Isah, A, McCollum, ED, Falade, AG, King, C, Iuliano, A, Burgess, RA, Agwai, I, Ahmar, S, Aranda, Z, Bahiru, S, Bakare, AA, Colbourn, T, Shittu, F, Graham, H, Isah, A, McCollum, ED, and Falade, AG
- Abstract
BACKGROUND: Interventions to reduce pneumonia mortality exist; however, stakeholder engagement is needed to prioritize these. We explored diverse stakeholder opinions on current policy challenges and priorities for pediatric pneumonia in Nigeria. METHODS: We conducted a mixed-methods study, with a web-survey and semi-structured interviews, to explore stakeholder roles, policy barriers, opportunities, and priorities. Web-survey participants were identified through stakeholder mapping, including researchers' networks, academic and grey literature, and "Every Breath Counts" coalition membership. Stakeholders included actors involved in pediatric pneumonia in Nigeria from non-governmental, government, academic, civil society, private, and professional organizations. Stakeholder interviews were conducted with local government, healthcare managers, professional associations, and local leaders in Lagos and Jigawa states. Quantitative data were analyzed descriptively; qualitative data were analyzed using a thematic framework. RESULTS: Of 111 stakeholders, 38 (34%) participated in the web-survey and 18 stakeholder interviews were conducted. Four thematic areas emerged: current policy, systems barriers, intervention priorities, and champions. Interviewees reported a lack of pneumonia-specific policies, despite acknowledging guidelines had been adopted in their settings. Barriers to effective pneumonia management were seen at all levels of the system, from the community to healthcare to policy, with key issues of resourcing and infrastructure. Intervention priorities were the strengthening of community knowledge and improving case management, focused on primary care. While stakeholders identified several key actors for pediatric pneumonia, they also highlighted a lack of champions. CONCLUSION: Consistent messages emerged to prioritize community and primary care initiatives, alongside improved access to oxygen, and pulse oximetry. There is a need for clear pneumonia policies, a
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- 2020
8. Community and caregivers' perceptions of pneumonia and care-seeking experiences in Nigeria: A qualitative study
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Bakare, AA, Graham, H, Agwai, IC, Shittu, F, King, C, Colbourn, T, Iuliano, A, Aranda, Z, McCollum, ED, Isah, A, Bahiru, S, Valentine, P, Falade, AG, Burgess, RA, MacCalla, M, Olowookere, TF, Cassar, C, Ahmed, T, Bianchi, V, Bakare, AA, Graham, H, Agwai, IC, Shittu, F, King, C, Colbourn, T, Iuliano, A, Aranda, Z, McCollum, ED, Isah, A, Bahiru, S, Valentine, P, Falade, AG, Burgess, RA, MacCalla, M, Olowookere, TF, Cassar, C, Ahmed, T, and Bianchi, V
- Abstract
BACKGROUND: Appropriate and timely care seeking can reduce pneumonia deaths, but are influenced by caregivers and community norms of health and illness. We explore caregiver and community perceptions, and care-seeking experience, of childhood pneumonia, to understand contexts that drive pediatric service uptake in Nigeria. METHODS: Community group discussions and qualitative interviews with caregivers in Lagos and Jigawa states were completed between 1 November 2018 and 31 May 2019. Participants were recruited from purposively sampled health facility catchment areas with assistance from facility staff. We used episodic interviews, asking caregivers (Jigawa = 20; Lagos = 15) to recount specific events linked to quests for therapy. Community group discussions (n = 3) used four vignettes from real pneumonia cases to frame a discussion around community priorities for healthcare and community-led activities to improve child survival. Data were analyzed using the framework method. RESULTS: We found poor knowledge of pneumonia-specific symptoms and risk factors among caregivers and community members, with many attributing pneumonia to cold air exposure. Interviews highlighted that care-seeking decision making involved both husbands and wives, but men often made final decisions. In Lagos, older female relatives also shaped quests for therapy. Cost was a major consideration. In both states, there were accounts of dissatisfaction with health workers' attitudes and a general acceptance of vaccination services. CONCLUSION: There is a need for community-based approaches to improve caregiver knowledge and care seeking for under-five children with pneumonia. Messaging should attend to knowledge of symptoms, risk factors, family dynamics, and community responsibilities in healthcare service delivery and utilization.
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- 2020
9. Inherited RORB pathogenic variants: Overlap of photosensitive genetic generalized and occipital lobe epilepsy
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Sadleir, LG, de Valles-Ibanez, G, King, C, Coleman, M, Mossman, S, Paterson, S, Nguyen, J, Berkovic, SF, Mullen, S, Bahlo, M, Hildebrand, MS, Mefford, HC, Scheffer, IE, Sadleir, LG, de Valles-Ibanez, G, King, C, Coleman, M, Mossman, S, Paterson, S, Nguyen, J, Berkovic, SF, Mullen, S, Bahlo, M, Hildebrand, MS, Mefford, HC, and Scheffer, IE
- Abstract
Variants in RORB have been reported in eight individuals with epilepsy, with phenotypes ranging from eyelid myoclonia with absence epilepsy to developmental and epileptic encephalopathies. We identified novel RORB variants in 11 affected individuals from four families. One was from whole genome sequencing and three were from RORB screening of three epilepsy cohorts: developmental and epileptic encephalopathies (n = 1021), overlap of generalized and occipital epilepsy (n = 84), and photosensitivity (n = 123). Following interviews and review of medical records, individuals' seizure and epilepsy syndromes were classified. Three novel missense variants and one exon 3 deletion were predicted to be pathogenic by in silico tools, not found in population databases, and located in key evolutionary conserved domains. Median age at seizure onset was 3.5 years (0.5-10 years). Generalized, predominantly absence and myoclonic, and occipital seizures were seen in all families, often within the same individual (6/11). All individuals with epilepsy were photosensitive, and seven of 11 had cognitive abnormalities. Electroencephalograms showed generalized spike and wave and/or polyspike and wave. Here we show a striking RORB phenotype of overlap of photosensitive generalized and occipital epilepsy in both individuals and families. This is the first report of a gene associated with this overlap of epilepsy syndromes.
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- 2020
10. Health system challenges for improved childhood pneumonia case management in Lagos and Jigawa, Nigeria
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Shittu, F, Agwai, IC, Falade, AG, Bakare, AA, Graham, H, Iuliano, A, Aranda, Z, McCollum, ED, Isah, A, Bahiru, S, Ahmed, T, Burgess, RA, King, C, Colbourn, T, MacCalla, M, Olowookere, TF, Ahmar, S, Cassar, C, Bianchi, V, Valentine, P, Shittu, F, Agwai, IC, Falade, AG, Bakare, AA, Graham, H, Iuliano, A, Aranda, Z, McCollum, ED, Isah, A, Bahiru, S, Ahmed, T, Burgess, RA, King, C, Colbourn, T, MacCalla, M, Olowookere, TF, Ahmar, S, Cassar, C, Bianchi, V, and Valentine, P
- Abstract
BACKGROUND: Case fatality rates for childhood pneumonia in Nigeria remain high. There is a clear need for improved case management of pneumonia, through the sustainable implementation of the Integrated Management of Childhood Illnesses (IMCI) diagnostic and treatment algorithms. We explored barriers and opportunities for improved case management of childhood pneumonia in Lagos and Jigawa states, Nigeria. METHODS: A mixed-method analysis was conducted to assess the current health system capacity to deliver quality care. This was done through audits of 16 facilities in Jigawa and 14 facilities in Lagos, questionnaires (n = 164) and 13 focus group discussions with providers. Field observations provided context for data analysis and triangulation. RESULTS: There were more private providers in Lagos (4/8 secondary facilities) and more government providers in Jigawa (4/8 primary, 3/3 secondary, and 1/1 tertiary facilities). Oxygen and pulse oximeters were available in two of three in Jigawa and six of eight in Lagos of the sampled secondary care facilities. None of the eight primary facilities surveyed in Jigawa had oxygen or pulse oximetry available while in Lagos two of three primary facilities had oxygen and one of three had pulse oximeters. Other IMCI and emergency equipment were also lacking including respiratory rate timers, particularly in Jigawa state. Health care providers scored poorly on knowledge of IMCI, though previous IMCI training was associated with better knowledge. Key enabling factors in delivering pediatric care highlighted by health care providers included accountability procedures and feedback loops, the provision of free medication for children, and philanthropic acts. Common barriers to provide care included the burden of out-of-pocket payments, challenges in effective communication with caregivers, delayed presentation, and lack of clear diagnosis, and case management guidelines. CONCLUSION: There is an urgent need to improve how the prevention a
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- 2020
11. The burden and risks of pediatric pneumonia in Nigeria: A desk-based review of existing literature and data
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Iuliano, A, Aranda, Z, Colbourn, T, Agwai, IC, Bahiru, S, Bakare, AA, Burgess, RA, Cassar, C, Shittu, F, Graham, H, Isah, A, McCollum, ED, Falade, AG, King, C, MacCalla, M, Olowookere, TF, Ahmar, S, Ahmed, T, Bianchi, V, Valentine, P, Iuliano, A, Aranda, Z, Colbourn, T, Agwai, IC, Bahiru, S, Bakare, AA, Burgess, RA, Cassar, C, Shittu, F, Graham, H, Isah, A, McCollum, ED, Falade, AG, King, C, MacCalla, M, Olowookere, TF, Ahmar, S, Ahmed, T, Bianchi, V, and Valentine, P
- Abstract
BACKGROUND: Pneumonia is a leading killer of children under-5 years, with a high burden in Nigeria. We aimed to quantify the regional burden and risks of pediatric pneumonia in Nigeria, and specifically the states of Lagos and Jigawa. METHODS: We conducted a scoping literature search for studies of pneumonia morbidity and mortality in under-5 children in Nigeria from 10th December 2018 to 26th April 2019, searching: Cochrane, PubMed, and Web of Science. We included grey literature from stakeholders' websites and information shared by organizations working in Nigeria. We conducted multivariable logistic regression using the 2016 to 2017 Multiple Cluster Indicators Survey data set to explore factors associated with pneumonia. Descriptive analyses of datasets from 2010 to 2019 was done to estimate trends in mortality, morbidity, and vaccination coverage. RESULTS: We identified 25 relevant papers (10 from Jigawa, 8 from Lagos, and 14 national data). None included data on pneumonia or acute respiratory tract infection burden in the health system, inpatient case-fatality rates, severity, or age-specific pneumonia mortality rates at state level. Secondary data analysis found that no household or caregiver socioeconomic indicators were consistently associated with self-reported symptoms of cough and/or difficulty breathing, and seasonality was inconsistently associated, dependant on region. CONCLUSION: There is a clear evidence gap around the burden of pediatric pneumonia in Nigeria, and challenges with the interpretation of existing household survey data. Improved survey approaches are needed to understand the risks of pediatric pneumonia in Nigeria, alongside the need for investment in reliable routine data systems to provide data on the clinical pneumonia burden in Nigeria.
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- 2020
12. Call for Hendra virus antibody testing
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King, C, primary
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- 2020
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13. Mapping and confirmation of quantitative trait loci (QTLs) associated with carbon isotope ratio (δ13C) in soybean
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Bazzer, Sumandeep K., primary, Kaler, Avjinder S., additional, King, C. Andy, additional, Ray, Jeffery D., additional, Hwang, Sadal, additional, and Purcell, Larry C., additional
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- 2020
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14. Registration of soybean germplasm lines R10‐2436 and R10‐2710 with drought tolerance traits and high yield under moderate water stress
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Manjarrez‐Sandoval, Pedro, primary, Chen, Pengyin, additional, Mozzoni, Leandro, additional, Florez‐Palacios, Liliana, additional, Orazaly, Moldir, additional, Wu, Chengjun, additional, Sinclair, Thomas R., additional, Carter, Thomas E., additional, Purcell, Larry C., additional, and King, C. Andy, additional
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- 2020
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15. The epileptology of GNB5 encephalopathy
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Poke, G, King, C, Muir, A, de Valles-Ibanez, G, Germano, M, Moura de Souza, CF, Fung, J, Chung, B, Fung, CW, Mignot, C, Ilea, A, Keren, B, Vermersch, A-I, Davis, S, Stanley, T, Moharir, M, Kannu, P, Shao, Z, Malerba, N, Merla, G, Mefford, HC, Scheffer, IE, Sadleir, LG, Poke, G, King, C, Muir, A, de Valles-Ibanez, G, Germano, M, Moura de Souza, CF, Fung, J, Chung, B, Fung, CW, Mignot, C, Ilea, A, Keren, B, Vermersch, A-I, Davis, S, Stanley, T, Moharir, M, Kannu, P, Shao, Z, Malerba, N, Merla, G, Mefford, HC, Scheffer, IE, and Sadleir, LG
- Abstract
Pathogenic variants in GNB5 cause an autosomal recessive neurodevelopmental disorder with neonatal sinus bradycardia. Seizures or epilepsy occurred in 10 of 22 previously reported cases, including 6 children from one family. We delineate the epileptology of GNB5 encephalopathy. Our nine patients, including five new patients, were from seven families. Epileptic spasms were the most frequent seizure type, occurring in eight of nine patients, and began at a median age of 3 months (2 months to 3 years). Focal seizures preceded spasms in three children, with onset at 7 days, 11 days, and 4 months. One child presented with convulsive status epilepticus at 6 months. Three children had burst suppression on electroencephalography (EEG), three had hypsarrhythmia, and one evolved from burst suppression to hypsarrhythmia. Background slowing was present in all after age 3 years. Magnetic resonance imaging (MRI) showed cerebral atrophy in one child and cerebellar atrophy in another. All nine had abnormal development prior to seizure onset and ultimately had profound impairment without regression. Hypotonia was present in all, with contractures developing in two older patients. All individuals had biallelic pathogenic variants in GNB5, predicted by in silico tools to result in protein truncation and loss-of-function. GNB5 developmental and epileptic encephalopathy is characterized by epileptic spasms, focal seizures, and profound impairment.
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- 2019
16. Epigenetic Reprogramming Driven By Metabolic Alterations As A Mechanism Of EGFR – Tyrosine Kinase Inhibitor Resistance In Human Lung Adenocarcinoma
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Xu Zhang, Tapan K Maity, Abhilash Venugopalan, Daniel R. Crooks, Vikram Misra, Nitin Roper, Andrew N. Lane, Andresson Thorkell, King C. Chan, W. Marston Linehan, Udayan Guha, Khoa Dang Nguyen, Constance M. Cultraro, Yue Qi, and Matthew Lynberg
- Subjects
Chemistry ,Mechanism (biology) ,Inhibitor resistance ,medicine.disease ,Biochemistry ,Human lung ,medicine.anatomical_structure ,Genetics ,medicine ,Cancer research ,Adenocarcinoma ,Molecular Biology ,Reprogramming ,Egfr tyrosine kinase ,Biotechnology - Published
- 2020
17. Inhibition of Testosterone Glucuronidation by Imatinib in Human Liver Microsomes Characterized for UGT2B17 Expression
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King C. Yabut, Hae Young Zhang, Abdul Basit, Carol Collins, and Bhagwat Prasad
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medicine.medical_specialty ,Human liver ,business.industry ,Glucuronidation ,Imatinib ,Testosterone (patch) ,030204 cardiovascular system & hematology ,Biochemistry ,03 medical and health sciences ,0302 clinical medicine ,Endocrinology ,Internal medicine ,Genetics ,medicine ,Microsome ,030212 general & internal medicine ,business ,Molecular Biology ,Biotechnology ,medicine.drug - Published
- 2018
18. Long‐Term Safety of Rituximab in Patients With Rheumatoid Arthritis: Results of a Five‐Year Observational Study
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Winthrop, Kevin L., primary, Saag, Kenneth, additional, Cascino, Matthew D., additional, Pei, Jinglan, additional, John, Ani, additional, Jahreis, Angelika, additional, Haselkorn, Tmirah, additional, Furst, Daniel E., additional, Abdulky, M., additional, Abeles, M., additional, Adelglass, H., additional, Ahmed, A., additional, Alloway, J., additional, Alper, J., additional, Anand, A., additional, Anderson, J., additional, Arora, M., additional, Askari, A., additional, Baca, S., additional, Bacha, D., additional, Bagheri, S., additional, Ballou, S., additional, Bennett, R., additional, Bidula, L., additional, Blumstein, H., additional, Bognar, M., additional, Bohan, A., additional, Boniske, C., additional, Borofsky, M., additional, Box, E., additional, Braun, A., additional, Brennan, T., additional, Brent, L., additional, Cabalar, I., additional, Carteron, N., additional, Chaudhary, K., additional, Chauhan, A., additional, Cima, M., additional, Cochinwala, A., additional, Cohen, H., additional, Colburn, K., additional, Conaway, D., additional, Danning, C., additional, Dao, K., additional, Dean, J., additional, Diab, I., additional, Diegel, R., additional, Ditzian‐Kadanoff, R., additional, Dowd, J., additional, Dugowson, C., additional, Eggebeen, A., additional, El‐Kadi, H., additional, Feinberg, H., additional, Feinman, M., additional, Feinstein, J., additional, Fischer, A., additional, Foad, B., additional, Fondal, M., additional, Fraser, S., additional, Fraser, A., additional, Freeman, P., additional, Garber, M., additional, Goldstein, A., additional, Golombek, S., additional, Greenstein, N., additional, Greenwald, M., additional, Hakim, C., additional, Halla, J., additional, Hallegua, D., additional, Han, K., additional, Harris, B., additional, Hauptman, H., additional, Hirsh, J., additional, Hoffman, M., additional, Huntwork, J., additional, Husni, M., additional, Hyer, F., additional, Hymowitz, R., additional, Jones, R., additional, Kanagasegar, S., additional, Kappes, J., additional, Keating, R., additional, Kelly, G., additional, Kim, J., additional, King, C., additional, Klashman, D., additional, Knee, C., additional, Kolba, K., additional, Krick, G., additional, Krug, H., additional, Kumar, U., additional, Lakhanpal, S., additional, Lang, T., additional, Lauter, S., additional, Lawrence Ford, T., additional, Lee, W., additional, Lee, Y., additional, Leisen, J., additional, Levine, J., additional, Lidman, R., additional, Lipstate, J., additional, Malinak, J., additional, Marcus, R., additional, Martin, D., additional, Mehta, C., additional, Melton, G., additional, Metyas, S., additional, Miller, K., additional, Moidel, R., additional, Moore, C., additional, Mossell, J., additional, Munoz, G., additional, Murphy, F., additional, Nami, A., additional, Nascimento, J., additional, Neal, N., additional, Neiman, R., additional, Neuwelt, C., additional, Nguyen, P., additional, Niemer, M., additional, Oelke, K., additional, Oza, M., additional, Pachaidee, S., additional, Patel, S., additional, Pegram, S., additional, Penmetcha, M., additional, Perkins, J., additional, Perl, A., additional, Peterson, L., additional, Pittsley, R., additional, Portnoff, K., additional, Rahmani, D., additional, Raja, N., additional, Ratnoff, W., additional, Rezaian, M., additional, Rhea, C., additional, Rice, D., additional, Ridley, D., additional, Rivadeneira, A., additional, Rizzo, W., additional, Roane, G., additional, Rocca, P., additional, Rosen, M., additional, Saikali, W., additional, Saitta, M., additional, Sankoorikal, A., additional, Saway, P., additional, Schneider, P., additional, Schwartzman, S., additional, Scoville, C., additional, Shergy, W., additional, Shiel, W, additional, Shurmur, R., additional, Sikes, D., additional, Singhal, A., additional, Snyder, A., additional, Songcharoen, S., additional, Sosenko, M., additional, Soto Raices, O., additional, Stahl, N., additional, Stark, K., additional, Strachan, M., additional, Stupi, A., additional, Sullivan, N., additional, Sylvester, R., additional, Tabechian, D., additional, Tagoe, C., additional, Taylor, P., additional, Thakker, S., additional, Thakor, M., additional, Thakur, N., additional, Tidmore, W., additional, Toth, M., additional, Trostle, D., additional, Udell, J., additional, Van de Stouwe, M., additional, Venuturupalli, R., additional, Weiss, D., additional, Weselman, K., additional, Winn, D., additional, Yung, C., additional, Zable, E., additional, and Zamiri, B., additional
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- 2019
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19. EVITA: PHASE I/II STUDY OF EVEROLIMUS PLUS ITACITINIB IN RELAPSED/REFRACTORY CLASSICAL HODGKIN LYMPHOMA
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Svoboda, J., primary, Barta, S., additional, Nasta, S., additional, Landsburg, D., additional, Gerson, J., additional, Ruella, M., additional, Waite, T., additional, King, C., additional, Emanuel, S.A., additional, Ballard, H., additional, and Schuster, S., additional
- Published
- 2019
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20. Novel immediate/sustained‐release formulation of acetaminophen‐ibuprofen combination (Paxerol®) for severe nocturia associated with overactive bladder: A multi‐center, randomized, double blinded, placebo‐controlled, 4‐arm trial
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Lee, King C., primary, Rauscher, Frank, additional, Kaminesky, Jed, additional, Ryndin, Igor, additional, Xie, Lei, additional, Zhao, Yunzhu, additional, Khusid, Johnathan A., additional, and Weiss, Jeffrey P., additional
- Published
- 2018
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21. Health-related quality of life of children and adolescents with cerebral palsy in low- and middle-income countries: a systematic review
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Power, R, King, C, Muhit, M, Heanoy, E, Galea, C, Jones, C, Badawi, N, Khandaker, G, Power, R, King, C, Muhit, M, Heanoy, E, Galea, C, Jones, C, Badawi, N, and Khandaker, G
- Abstract
AIM: To systematically review literature on health-related quality of life (HRQoL) of children and adolescents (≤18yo) with cerebral palsy (CP) from low- and middle-income countries (LMICs) to identify trends in HRQoL and areas for future research. METHOD: We systematically reviewed six key bibliographic databases and two reviewers independently screened results. Peer-reviewed original articles examining HRQoL of children from LMICs were eligible. RESULTS: A total of 22 524 papers were identified, of which 16, from eight LMICs, were included. Four measures of HRQoL were used; Child Health Questionnaire Parent Form 50 (n=5); Paediatric Quality of Life Inventory 3.0 and 4.0 (n=4); CP Quality of Life Questionnaire for Children (n=4); Lifestyle Assessment Questionnaire CP (n=3). Children with CP from LMICs (n=1579; 2-18y) had significantly poorer HRQoL on all instrument dimensions when compared to age-matched controls (p<0.003) and on all except two dimensions when compared to peers in high-income countries (p<0.001). Physical well-being dimensions of HRQoL were poorest overall and associated with impaired motor function. INTERPRETATION: Research to improve HRQoL in LMICs is required and should address all aspects of HRQoL. Future research is recommended to incorporate multi-respondent assessment, utilize both general and CP-specific measures of HRQoL, and delineate adolescents as a unique cohort. What this paper adds Children with cerebral palsy from low- and middle-income countries are at high risk of poor health-related quality of life (HRQoL). Physical well-being was poorest dimension of HRQoL and associated with impaired motor function.
- Published
- 2018
22. Routine splenic flexure mobilization may increase compliance with pathological quality metrics in patients undergoing low anterior resection
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Mouw, T. J., primary, King, C., additional, Ashcraft, J. H., additional, Valentino, J. D., additional, DiPasco, P. J., additional, and Al‐Kasspooles, M., additional
- Published
- 2018
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23. A multifaceted approach towards interpreting early life experience and infant feeding practices in the ancient Atacama Desert, Northern Chile
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King, C. L., primary, Snoddy, A. M., additional, Millard, A. R., additional, Gröcke, D. R., additional, Standen, V. G., additional, Arriaza, B. T., additional, and Halcrow, S. E., additional
- Published
- 2018
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24. Effects of fat supplementation on plasma glucose, insulin and fatty acid analysis in ponies maintained on a forage-based diet
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Williams, T., primary, Rude, B., additional, Liao, S., additional, Mochal-King, C., additional, and Nicodemus, M., additional
- Published
- 2018
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25. Computed tomography scanner dimensions
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King, C., primary and Barry, A., additional
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- 2018
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26. Global proteomics and metabolomics in cancer biomarker discovery
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Stephen D. Fox, Haleem J. Issaq, Timothy D. Veenstra, and King C. Chan
- Subjects
Chromatography ,Metabolomics ,Chemistry ,Electrospray ionization ,Difference gel electrophoresis ,Proteome ,Quantitative proteomics ,Metabolome ,Filtration and Separation ,Biomarker discovery ,Proteomics ,Analytical Chemistry - Abstract
Chromatography and electrophoresis have been used for the last half-century to separate small and large molecules. Advances in MS instrumentation and techniques for sample introduction into the mass analyzer (i.e. matrix-assisted laser desorption/ionization and electrospray ionization), chromatography in all its formats and modes and two-dimensional gel electrophoresis, including two-dimensional difference gel electrophoresis, enabled the separation of complex biological mixtures, such as the proteome and the metabolome, in a biological sample. These advances have made it possible to identify compounds that can be used to discriminate between two samples taken from healthy and diseased individuals. The objective is to find proteins or metabolites that can be used as a clinical test for the early diagnosis, prognosis and monitoring of the disease and the outcome of therapy. In this manuscript, we present an overview of what has been achieved in the search for biomarkers, with emphasis on cancer, using separation science and MS.
- Published
- 2011
27. Genome‐Wide Association Mapping of Carbon Isotope and Oxygen Isotope Ratios in Diverse Soybean Genotypes
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Kaler, Avjinder S., primary, Dhanapal, Arun P., additional, Ray, Jeffery D., additional, King, C. Andy, additional, Fritschi, Felix B., additional, and Purcell, Larry C., additional
- Published
- 2017
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28. Parental disease prevention health beliefs and triggers for keeping children home from childcare—a qualitative study in Sydney, Australia
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King, C. L., primary and Leask, J., additional
- Published
- 2017
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29. Cdh13andAdipoQgene knockout alter instrumental and Pavlovian drug conditioning
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King, C. P., primary, Militello, L., additional, Hart, A., additional, St. Pierre, C. L., additional, Leung, E., additional, Versaggi, C. L., additional, Roberson, N., additional, Catlin, J., additional, Palmer, A. A., additional, Richards, J. B., additional, and Meyer, P. J., additional
- Published
- 2017
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30. Evaluation of Methods for Estimating Transpiration Response to Soil Drying for Container‐Grown Plants
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King, C. Andy, primary and Purcell, Larry C., additional
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- 2017
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31. Analysis of fullerene-based nanomaterial in serum matrix by CE
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Haleem J. Issaq, Scott E. McNeil, Anil K. Patri, Timothy D. Veenstra, and King C. Chan
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Serum ,Detection limit ,Biodistribution ,Chromatography ,Resolution (mass spectrometry) ,Chemistry ,Clinical Biochemistry ,Electrophoresis, Capillary ,Reproducibility of Results ,Nanoparticle ,Buffers ,Hydrogen-Ion Concentration ,Sensitivity and Specificity ,Biochemistry ,Micelle ,Nanostructures ,Analytical Chemistry ,Matrix (chemical analysis) ,Electrophoresis ,Calibration ,Drug delivery ,Humans ,Indicators and Reagents ,Fullerenes ,Chromatography, Micellar Electrokinetic Capillary - Abstract
With the increasing interest in using nanoparticles as vehicles for drug delivery and image contrast agents, there is a need to develop assays for their detection and quantitation in complex matrices to facilitate monitoring their biodistribution. In this study, we developed a CE approach for the analysis of two nanoparticles: carboxyfullerene (C3) and dendrofullerene (DF1) in both standard solutions and a serum matrix. These highly soluble, charged C(60) derivatives were characterized by CZE using either a bare or dynamically coated fused-silica capillaries. The resolution of both nanoparticles was slightly lower with the coated capillary; however, their migration times were faster. While separation of the DF1 nanoparticles using MEKC resulted in a greater number of observable peaks, the peak profile of C3 was basically unchanged regardless of whether SDS micelles were added to the running buffers or not. The MEKC and/or CZE assays were then used to quantitate the C3 and DF1 nanoparticles in spiked human serum samples. The quantitation of the nanoparticles was linear from 0-500 microg/mL with detection limits ranging from 0.5 to 6 microg/mL.
- Published
- 2007
32. Analysis of the extracellular matrix vesicle proteome in mineralizing osteoblasts
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Corinne E. Camalier, Haleem J. Issaq, Thomas P. Conrads, King C. Chan, Stephen J. Lockett, George R. Beck, Timothy D. Veenstra, David A. Lucas, Kunio Nagashima, Zhen Xiao, M. Jason de la Cruz, and Michelle Gignac
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Proteomics ,Proteome ,Physiology ,Clinical Biochemistry ,Bone Matrix ,Fluorescent Antibody Technique ,Biology ,Mass Spectrometry ,Cell Line ,Extracellular matrix ,Mice ,Calcification, Physiologic ,Microscopy, Electron, Transmission ,Osteogenesis ,medicine ,Animals ,Secretion ,Extracellular Matrix Proteins ,Bone Development ,Osteoblasts ,Vesicle ,Cytoplasmic Vesicles ,Osteoblast ,Cell Biology ,Cell biology ,medicine.anatomical_structure ,Biochemistry ,Microscopy, Electron, Scanning ,Signal transduction ,Biogenesis - Abstract
Many key processes central to bone formation and homeostasis require the involvement of osteoblasts, cells responsible for accumulation and mineralization of the extracellular matrix (ECM). During this complex and only partially understood process, osteoblasts generate and secrete matrix vesicles (MVs) into the ECM to initiate mineralization. Although they are considered an important component of mineralization process, MVs still remain a mystery. To better understand their function and biogenesis, a proteomic analysis of MVs has been conducted. MVs were harvested by two sample preparation approaches and mass spectrometry was utilized for protein identification. A total of 133 proteins were identified in common from the two MV preparations, among which were previously known proteins, such as annexins and peptidases, along with many novel proteins including a variety of enzymes, osteoblast-specific factors, ion channels, and signal transduction molecules, such as 14-3-3 family members and Rab-related proteins. To compare the proteome of MV with that of the ECM we conducted a large-scale proteomic analysis of collagenase digested mineralizing osteoblast matrix. This analysis resulted in the identification of 1,327 unique proteins. A comparison of the proteins identified from the two MV preparations with the ECM analysis revealed 83 unique, non-redundant proteins identified in all three samples. This investigation represents the first systematic proteomic analysis of MVs and provides insights into both the function and origin of these important mineralization-regulating vesicles.
- Published
- 2007
33. Long-term outcomes of infective encephalitis in children: a systematic review and meta-analysis
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Khandaker, G, Jung, J, Britton, PN, King, C, Yin, JK, Jones, CA, Khandaker, G, Jung, J, Britton, PN, King, C, Yin, JK, and Jones, CA
- Abstract
AIM: The long-term outcomes of childhood infective encephalitis are variable and not well quantified. We aimed to systematically review the literature and undertake meta-analyses on predetermined outcomes to address this knowledge gap and identify areas for future research. METHOD: We searched electronic databases, performed complementary reviews of references of fully extracted articles, and made contact with experts on infective encephalitis. Articles published up until April 2016 were selected for screening. RESULTS: We evaluated sequelae of 1018 survivors of childhood infective encephalitis (934 with complete follow-up) from 16 studies. Mean age during acute encephalitis episodes was 5 years 3.6 months (range 1.2mo-17y), 57.6% were male (500/868), and mean follow-up period was 4 years 1.2 months (range 1-12y). Incomplete recovery was reported in 312 children (42.0%; 95% confidence interval [CI] 31.6-53.1% in pooled estimate). Among the other sequelae, developmental delay, abnormal behaviour, motor impairment, and seizures were reported among 35.0% (95% CI 10.0-65.0%), 18.0% (95% CI 8.0-31.0%), 17.0% (95% CI 10.0-26.0%), and 10.0% (95% CI 6.0-14.0%) respectively. INTERPRETATION: Almost half of childhood infective encephalitis survivors report incomplete recovery in the long-term; most commonly developmental delay, behavioural abnormality, and neurological impairments (i.e. seizure). Well designed, large-scale prospective studies are needed to better quantify neurodevelopmental sequelae among childhood encephalitis survivors.
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- 2016
34. Quantitative proteomic analysis of inorganic phosphate-induced murine MC3T3-E1 osteoblast cells
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David A. Lucas, George R. Beck, Kelly A. Conrads, King C. Chan, Ming Zhou, Timothy D. Veenstra, Haleem J. Issaq, Carl F. Schaefer, Thomas P. Conrads, Kerri A. Simpson, and Li-Rong Yu
- Subjects
Proteome ,Molecular Sequence Data ,Clinical Biochemistry ,Quantitative proteomics ,Peptide ,Proteomics ,Biochemistry ,Phosphates ,Analytical Chemistry ,Mice ,Cyclin D1 ,Isotopes ,Animals ,Amino Acid Sequence ,Peptide sequence ,Cells, Cultured ,chemistry.chemical_classification ,Osteoblasts ,biology ,Avidin ,Molecular biology ,Blot ,Gene Expression Regulation ,chemistry ,Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ,biology.protein ,Chromatography, Liquid - Abstract
Cleavable isotope-coded affinity tag (cICAT) reagents were utilized to identify and quantitate protein expression differences in control and inorganic phosphate-treated murine MC3T3-E1 osteoblast cells. Proteins extracted from control and treated cells were labeled with the light and heavy isotopic versions of cICAT reagents, respectively. The cICAT-labeled samples were combined, proteolytically digested, and the cICAT-derivatized peptides isolated using immobilized avidin chromatography. The cICAT-labeled peptides were resolved into 96 fractions by strong cation-exchange (SCX) liquid chromatography (LC). Analysis of the SCX-LC cICAT peptide fractions by microcapillary reversed-phase LC-tandem mass spectrometry resulted in the identification and quantitation of 7227 unique peptides corresponding to 2501 proteins, or roughly 9% of the proteins currently predicted to be encoded by the mouse genome. A false positive analysis indicated a 98% confidence in the peptide identifications. To corroborate changes in abundance measured by cICAT with those detectable in traditionally prepared cell lysate, we chose to analyze cyclin D1. Cyclin D1 has been previously identified as a phosphate-responsive gene and was likewise identified as a phosphate-responsive protein in the current analysis. The 1.76-fold increase in abundance in cyclin D1 determined from cICAT corresponds well with the 2.41-fold increase as determined by Western blotting. These results demonstrate that quantitative proteomics is capable of providing a quantitative view of thousands of proteins in mammalian cells within a defined set of experiments.
- Published
- 2004
35. Electrically driven microseparation methods for pesticides and metabolites: VI. Surfactant-mediated electrokinetic capillary chromatography of aniline pesticidic metabolites derivatized with 9-fluoroenylmethyl chloroformate and their detection by laser-induced fluorescence
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William Wall, King C. Chan, and Ziad El Rassi
- Subjects
Detection limit ,Chromatography ,Fluorenes ,Aniline Compounds ,Lasers ,Clinical Biochemistry ,Electrophoresis, Capillary ,Fresh Water ,Chloroformate ,Biochemistry ,Analytical Chemistry ,Matrix (chemical analysis) ,Electrolytes ,Surface-Active Agents ,chemistry.chemical_compound ,Spectrometry, Fluorescence ,Capillary electrophoresis ,chemistry ,Pulmonary surfactant ,Pesticides ,Derivatization ,Laser-induced fluorescence ,Acetonitrile ,Water Pollutants, Chemical - Abstract
In this report, we describe a surfactant-mediated electrokinetic capillary chromatography (SM-EKC) system for the separation of 9-fluoroenylmethyl chloroformate (FMOC)-derivatized anilines by capillary electrophoresis (CE). The SM-EKC system consisted of dioctyl sulfosuccinate (DOSS)/acetonitrile mixtures and was suited for the CE separation of the relatively hydrophobic FMOC-aniline analytes and other neutral compounds, e.g. alkylphenyl ketones. While the organic modifier acetonitrile (ACN) allowed the solubilization of the hydrophobic solutes and maintained the DOSS surfactant in its monomeric form by inhibiting micellization, the DOSS surfactant associated with the FMOC anilines to a varying degree thus leading to their differential migration and separation. Under these conditions, the FMOC-anilines were readily detected at the 10(-6) M level by UV at 214 nm and at the 10(-8) M level by laser-induced fluorescence (LIF) using a solid-state UV laser operating at 266 nm line as the excitation wavelength. The FMOC precolumn derivatization was also readily performed in lake water spiked with anilines at near the limit of detection (LOD) level. The lake water matrix showed no significant effects on the extent of derivatization at the LOD level as well as on the detection of the analytes due to the selectivity of the FMOC derivatization. The derivatization and detection of spiked lake water necessitated only the removal of microparticles by microfiltration prior to derivatization and detection.
- Published
- 2001
36. Solid-state UV laser-induced fluorescence detection in capillary electrophoresis
- Author
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Haleem J. Issaq, King C. Chan, and Gary M. Muschik
- Subjects
chemistry.chemical_classification ,Chromatography ,biology ,Chemistry ,Cytochrome c ,Clinical Biochemistry ,Analytical chemistry ,Laser ,Fluorescamine ,Biochemistry ,Fluorescence ,Analytical Chemistry ,law.invention ,Amino acid ,chemistry.chemical_compound ,Capillary electrophoresis ,law ,biology.protein ,Laser-induced fluorescence ,Derivatization - Abstract
Two solid-state UV lasers were applied to the laser-induced fluorescence (LIF) detection of various groups of compounds after separation by capillary electrophoresis. These lasers are thermoelectric-cooled, highly compact, and inexpensive. Such lasers provide few mW of quasi-continuous wave (CW) power which are sufficient and stable for LIF detection. Native fluorescence detection of tryptophan-containing proteins and peptides and related indoles was achieved at the nM level with the laser operating at 266 nm. Detection of fluorescamine-labeled amino acids and peptides was also possible at the nM level with the laser operating at 355 nm. Amino acids at a concentration as low as 10 ng/mL could be labeled with fluorescamine. Solid-state UV-LIF detection of the tryptic digest of cytochrome c after fluorescamine derivatization was demonstrated.
- Published
- 2000
37. A simple two-dimensional high performance liquid chromatography/high performance capillary electrophoresis set-up for the separation of complex mixtures
- Author
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Gary M. Muschik, Haleem J. Issaq, King C. Chan, and George M. Janini
- Subjects
Free-flow electrophoresis ,Capillary electrochromatography ,Chromatography ,Chemistry ,Clinical Biochemistry ,Analytical chemistry ,medicine.disease_cause ,Mass spectrometry ,Biochemistry ,Fluorescence ,Capillary electrophoresis–mass spectrometry ,High-performance liquid chromatography ,Analytical Chemistry ,Capillary electrophoresis ,medicine ,Ultraviolet - Abstract
A two-dimensional high performance liquid chromatography/capillary electrophoresis (HPLC/CE) instrumental set-up was assembled from commercially available equipment. Fractions of the effluent from the HPLC system are collected into microtiter plates with a microfraction collector. The fractions are then dried under vacuum at room temperature, reconstituted, and analyzed by capillary zone electrophoresis (CZE). This method allows the collection of samples by time, drops, or external signal (peaks). Any size or type of HPLC or CE column can be used with no limitation on the amount of sample injected into the HPLC. Any CE detection, laser-induced fluorescence (LIF), mass spectrometry (MS), ultraviolet (UV) or other, can be used. This set-up is practical, simple, robust and allows the separation of complex mixtures. Preliminary results show the utility of this system for the analysis of protein digest.
- Published
- 1999
38. Genome‐Wide Association Analysis of Diverse Soybean Genotypes Reveals Novel Markers for Nitrogen Traits
- Author
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Dhanapal, Arun Prabhu, primary, Ray, Jeffery D., additional, Singh, Shardendu K., additional, Hoyos‐Villegas, Valerio, additional, Smith, James R., additional, Purcell, Larry C., additional, King, C. Andy, additional, and Fritschi, Felix B., additional
- Published
- 2015
- Full Text
- View/download PDF
39. Multidimensional high performance liquid chromatography - capillary electrophoresis separation of a protein digest: An update
- Author
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Qingbo Li, Haleem J. Issaq, King C. Chan, and ChangSheng Liu
- Subjects
Free-flow electrophoresis ,Capillary electrochromatography ,Chromatography ,Chemistry ,Clinical Biochemistry ,Analytical chemistry ,Gel electrophoresis of proteins ,Biochemistry ,High-performance liquid chromatography ,Analytical Chemistry ,Electropherogram ,Electrophoresis ,Microtiter plate ,Capillary electrophoresis - Abstract
The trypsin digest of a mixture of two proteins, namely cytochrome c and myoglobin, was first separated in the first dimension by high-performance liquid chromatography (HPLC). Fractions from the HPLC were collected every 30s with the aid of a fraction collector into a 96-well microtiter plate. After concentration, all the collected fractions were analyzed simultaneaosly in the second dimension by a 96-array capillary electrophoresis system. The labeled peptides were detected by laser-induced fluorescence. An internal standard, allura red, was added to all the fractions, prior to capillary electrophoretic analysis. The internal standard serves two functions, migration time correction and signal intensity correction. The data are presented in two different formats, as an electropherogram of all the fractions and in a two-dimensional (2-D) format. The 2-D plot of the data shows the density of each spot, which corresponds to the concentration of the migrating peptides. The total experimental time for the HPLC and capillary electrophoretic analyses ist less than 1 h, which ist much faster than using 2-D slab-gel electrophoresis or single-capillary capillary electrophoresis.
- Published
- 2001
40. Registration of the KS4895 × Jackson Soybean Mapping Population, AR93705
- Author
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Hwang, Sadal, primary, King, C. Andy, additional, Davies, Marilynn K., additional, Charlson, Dirk V., additional, Ray, Jeffery D., additional, Cregan, Perry B., additional, Sneller, Clay H., additional, Chen, Pengyin, additional, Carter, Thomas E., additional, and Purcell, Larry C., additional
- Published
- 2015
- Full Text
- View/download PDF
41. In vivo and in vitro effects of the novel antiarrhythmic ipazilide on cardiac and vascular smooth muscle function
- Author
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Alan M. Ezrin, Alex L. Harris, King C. Lee, and Paul J. Silver
- Subjects
Cardiac output ,medicine.medical_specialty ,Vascular smooth muscle ,business.industry ,Sotalol ,Cardiac muscle ,Contractility ,Preload ,medicine.anatomical_structure ,Internal medicine ,Drug Discovery ,medicine ,Cardiology ,Vascular resistance ,Disopyramide ,business ,medicine.drug - Abstract
Ipazilide fumarate is a novel antiarrhythmic currently undergoing clinical evaluation. Since most antiarrhythmics have adverse cardiovascular side effects, we examined the potential effects of ipazilide on cardiac muscle and vascular hemodynamic function utilizing in vivo and in vitro models. The cardiovascular effects of ipazilide and selected reference antiarhythmics, disopyramide and sotalol, at relevant antiarrhythmic concentrations were compared. All agents decreased cardiac contractility, as evident by reductions in dP/dt and cardiac output in anesthetized dogs, and by decreases in contractile force in isolated guinea pig papillary muscles. In vivo, ipazilide was approximately 3–10 × less potent than disopyramide or sotalol while in vitro, ipazilide produced a greater decrease in contractility at a relatively high concentration (30 μM). All three agents increased cardiac preload as reflected by increases in left ventricular end diastolic pressure and right atrial pressure, with ipazilide 2–3 × less potent than disopyramide or sotalol. Differential effects on vascular function were also evident with the three agents. Disopyramide and sotalol significantly increased systemic vascular resistance, and either pulmonary vascular resistance (disopyramide) or left coronary arterial vascular resistance (sotalol); ipazilide did not significantly affect any of these parameters. In isolated rat arterial or canine coronary arterial smooth muscle, ipazilide produced concentration-related vasorelaxation, with EC50 values ranging from 11–300 μM. Efficacy of ipazilide was higher in depolarized vascular tissue. In conclusion, since previous preclinical studies have indicated equal or greater antiarrhythmic activity of ipazilide relative to disopyramide or sotalol, the current data indicate the possibility for lessened in vivo hemodynamic effects of ipazilide at efficacious antiarrhythmic dosages. © 1992 Wiley-Liss, Inc.
- Published
- 1992
42. Myocardial salvage by trolox and ascorbic acid, but not ascorbic acid alone, in anesthetized dogs and rabbits
- Author
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Patrick Horan, King C. Lee, Paul C. Canniff, Alan M. Ezrin, and Paul J. Silver
- Subjects
business.industry ,Vitamin E ,medicine.medical_treatment ,Ischemia ,Hemodynamics ,medicine.disease ,Ascorbic acid ,chemistry.chemical_compound ,chemistry ,Anesthesia ,Drug Discovery ,Occlusion ,medicine ,Trolox ,Myocardial infarction ,business ,Reperfusion injury - Abstract
The myocardial salvaging properties of Trolox (Trix; a water- and lipid-soluble vitamin E analog with antioxidant properties) and ascorbic acid (Asc; a water-soluble antioxidant) were evaluated in anesthetized male dogs and rabbits. Myocardial infarction (MI) was induced by occlusion and reperfusion of the left anterior descending coronary artery: a 2-hr occlusion and 4-hr reperfusion in dogs, and a 15-min occlusion and 3-hr reperfusion in rabbits. This occlusion/reperfusion protocol induced %MI (MI normalized to area at risk) of approximately 20% beyond that induced by occlusion alone in both species. Trlx, Asc (100 and 150 mg/kg/injection, respectively, by injection; or 100 and 150 mg/kg/min, respectively, by continuous infusion), or vehicle (Veh) were administered into the ascending aorta in dogs and into the left atrium in rabbits. In severely ischemic dogs (myocardial collateral blood flow < 0.1 ml/min/g), a single injection of Trlx plus Asc, but not Asc alone, at the onset of reperfusion reduced %MI (Trlx + Asc = 24.3 ± 5.4%, n = 7; Asc = 50.1 ± 9.6%, n = 4; Veh = 45.0 ± 5.0%, n = 5). In rabbits, %MI was reduced (or tended to be reduced) by either 3 hourly injections (Trlx + Asc = 33.3 ± 4.0%, n = 19; Asc = 48.3 ± 6.3%, n = 11; Veh = 43.7 ± 3.3%, n = 8) or an injection followed by continuous infusion (Trlx + Asc = 36.0 ± 4.0%, n = 9; Veh = 49.0 ± 3.0%, n = 8), beginning at the onset of reperfusion. %MI in rabbits was not affected by either hourly injections beginning from 1 hr after, or a single injection at 1–3 × the dosages (i.e., 100–300 and 150–450 mg/kg for Trlx and Asc, respectively), during onset of reperfusion. Hemodynamic variables (arterial pressure, heartrate, rate-pressure-product and left ventricular contractility) were similar among all treat- ment groups in both species. In conclusion, Trlx/Asc combination, but not Asc alone, re- duces %MI in dogs and rabbits. The salvaging effects of Trlx/Asc are unrelated to any changes in hemodynamics. These results are consistent with the hypothesis that lipid peroxidation is involved in myocardial reperfusion injury. © 1992 Wiley-Liss, Inc.
- Published
- 1992
43. Comparative hemodynamic and renal effects of the low Km cGMP phosphodiesterase inhibitors cicletanine and zaprinast in anesthetized dogs
- Author
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King C. Lee, Douglas W. Hamel, William P. Gorcyzca, Edward D. Pagani, Paul C. Canniff, Alan M. Ezrin, and Paul J. Silver
- Subjects
medicine.medical_specialty ,Cicletanine ,Chemistry ,Hemodynamics ,Diuresis ,chemistry.chemical_compound ,Endocrinology ,Nadolol ,Renal blood flow ,Internal medicine ,Drug Discovery ,Ventricular pressure ,medicine ,Arterial blood ,Zaprinast ,medicine.drug - Abstract
Cicletanine, a vasorelaxant/natriuretic agent, inhibits low Km cGMP/phosphodiesterase (PDE) (IC50 = 300 μ). The objectives of this study were to compare the cardiovascular and renal effects of cicletanine with another cGMP-PDE inhibitor, zaprinast (IC50 = 0.3 μ), at intravenous dosages of 0.3–3 mg/kg, and to examine the influence of β-adreno-receptor blockade (nadolol, 1 mg/kg, i.v.) upon the responses to cicletanine in anesthetized dogs. Cicletanine (3 mg/kg) and zaprinast (0.3−3 mg/kg) decreased mean arterial pressure (−21.2±3.9 mm Hg [mean±SEM, max. change] at 3 mg/kg and −64.7 ± 4.8 mm Hg at 0.3 mg/kg, respectively). The response curve of zaprinast was negatively related to the dosages. Cardiac output was increased or tended to increase by approximately 0.3 liter/min by both cicletanine and zaprinast at 3 mg/kg. Renal, but not femoral, blood flow was increased by both cicletanine and zaprinast (18.8±3.7 ml/min at 3 mg/kg and 17.0±4.6 ml/min at 1 mg/kg, respectively). These agents did not have major effects upon heart rate, left ventricular end diastolic pressure, and maximum rates of rise and decline in left ventricular pressure. These agents also did not affect pulmonary systolic, mean and diastolic arterial pressures, nor arterial blood pO2, pCO2, and pH. Both cicletanine and zaprinast increased or tended to increase urinary output (0.66±0.05 and 0.43±0.22 ml/min, respectively, both at 3 mg /kg) and urinary Na+ excretion (150.4±19.7 and 67.0±30.9 μEq/min, respectively, both at 3 mg/kg). Neither agents affected urinary K+ excretion. None of the responses to cicletanine were altered by β-, suggesting that β- adrenoreceptors were not in volved in mediating the affects of cicletanine. In conclusion, the cGMP-PDE inhibitors cicletanine and zaprinast vasodilate, increase renal blood flow, and induce diuresis and natrluresis in anesthetized dogs. However, the differences in the natriuretic and vasodilator effects of these agents cannot be explained by the difference in their potencies in PDE inhibition, suggesting that their effects in these dogs may involve additional mechanisms of action.
- Published
- 1991
44. Cardiovascular effects of medorinone in ?-adrenoreceptor-blocked and non-blocked anesthetized dogs
- Author
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King C. Lee, Dorothy J. Fort, Douglas W. Hamel, Paul C. Canniff, Edward D. Pagani, Alan M. Ezrin, and Paul J. Silver
- Subjects
Cardiac output ,Mean arterial pressure ,medicine.medical_specialty ,business.industry ,Hemodynamics ,Preload ,Blood pressure ,Endocrinology ,Internal medicine ,Renal blood flow ,Drug Discovery ,Ventricular pressure ,Medicine ,Milrinone ,business ,medicine.drug - Abstract
The hemodynamic effects of the low Km cAMP peak III PDE inhibitor medorinone (0.01–0.3 mg/kg, i. v. ) were evaluated in anesthetized dogs in the presence and absence of β-adrenoreceptor blocked. Medorinone increased the peak derivative of left ventricular pressure (+ dP/dt) in non-blocked (all doses) and β-blocked dogs (≥0.03 mg/kg) (2,766±259 and 1,403±262 mm Hg/sec, respectice max. changes). Heart rate (HR) was increased by medorinone in non-blocked (≥0.1 mg/kg) and β-blocked dogs (≥0.03 mg/kg) (77.4±8.9 and 25.5±4.3 beats/min, respective max. changes). In non-blocked dogs only, medorinone (all doses) decreased left ventricular end-diastolic pressure (LVEDP) (7.7±1.7 mm Hg, max. change). Mean arterial pressure (MAP) was similarly decreased by medorinone (
- Published
- 1990
45. SU‐E‐J‐179: Prediction of Pelvic Nodal Coverage Using Mutual Information Between Cone‐Beam and Planning CTs
- Author
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Jani, S, primary, Kishan, A, additional, O' Connell, D, additional, King, C, additional, Steinberg, M, additional, Low, D, additional, and Lamb, J, additional
- Published
- 2014
- Full Text
- View/download PDF
46. Two Soybean Plant Introductions Display Slow Leaf Wilting and Reduced Yield Loss under Drought
- Author
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Pathan, S. M., primary, Lee, J.-D., additional, Sleper, D. A., additional, Fritschi, F. B., additional, Sharp, R. E., additional, Carter, T. E., additional, Nelson, R. L., additional, King, C. A., additional, Schapaugh, W. T., additional, Ellersieck, M. R., additional, Nguyen, H. T., additional, and Shannon, J. G., additional
- Published
- 2014
- Full Text
- View/download PDF
47. A Possible Relationship Between Shoot N Concentration and the Sensitivity of N2Fixation to Drought in Soybean
- Author
-
King, C. Andy, primary, Purcell, Larry C., additional, Bolton, Alejandro, additional, and Specht, James E., additional
- Published
- 2014
- Full Text
- View/download PDF
48. Assessment of nutritional status in clinical settings
- Author
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Langley‐Evans, S., primary and King, C. R., additional
- Published
- 2014
- Full Text
- View/download PDF
49. Nineteen years of management for Phoenicopteriformes in European Association of Zoos and Aquaria institutions: the Fabulous Flamingo Surveys and strategies to increase reproduction in captivity
- Author
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King, C. E., primary and Bračko, A., additional
- Published
- 2013
- Full Text
- View/download PDF
50. QTL Analysis of Shoot Ureide and Nitrogen Concentrations in Soybean [ Glycine max (L.) Merr.]
- Author
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Hwang, Sadal, primary, King, C. Andy, additional, Davies, Marilynn K., additional, Ray, Jeffery D., additional, Cregan, Perry B., additional, and Purcell, Larry C., additional
- Published
- 2013
- Full Text
- View/download PDF
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