31 results on '"Kermit L. Carraway"'
Search Results
2. Profiling Bis(monoacylglycero)phosphate Lipids in Cancer Cell Lysosomes as Therapeutic Targets
- Author
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Megan R. Showalter, Anastasia L. Berg, Michael Sa, Oliver Fiehn, Tobias Kind, Hiroshi Tsugawa, Kermit L. Carraway, and Kacey VanderVorst
- Subjects
Biochemistry ,Chemistry ,Cancer cell ,Genetics ,Bis(monoacylglycero)phosphate ,Molecular Biology ,Biotechnology - Published
- 2018
3. MUC4 involvement in ErbB2/ErbB3 phosphorylation and signaling in response to airway cell mechanical injury
- Author
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George Theodoropoulos, Coralie A. Carothers Carraway, and Kermit L. Carraway
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Receptor, ErbB-2 ,Immunoprecipitation ,Blotting, Western ,Immunocytochemistry ,Fluorescent Antibody Technique ,Respiratory Mucosa ,Biochemistry ,Receptor tyrosine kinase ,Cell Line ,Epithelial Damage ,Humans ,Phosphorylation ,skin and connective tissue diseases ,Receptor ,Molecular Biology ,Protein kinase B ,Mucin-4 ,biology ,Epithelial Cells ,Cell Biology ,Immunohistochemistry ,Cell biology ,biology.protein ,Respiratory epithelium ,Stress, Mechanical ,sense organs ,Signal Transduction - Abstract
The receptor tyrosine kinases ErbB2 and ErbB3 are phosphorylated in response to injury of the airway epithelium. Since we have shown that the membrane mucin MUC4 can act as a ligand/modulator for ErbB2, affecting its localization in polarized epithelial cells and its phosphorylation, we questioned whether Muc4 was involved, along with ErbB2 and ErbB3, in the damage response of airway epithelia. To test this hypothesis, we first examined the localization of MUC4 in human airway samples. Both immunocytochemistry and immunofluorescence showed a co-localization of MUC4 and ErbB2 at the airway luminal surface. Sequential immunoprecipitation and immunoblotting from airway cells demonstrated that the MUC4 and ErbB2 are present as a complex in airway epithelial cells. To assess the participation of MUC4 in the damage response, cultures of NCI-H292 or airway cells were scratch-wounded, then analyzed for association of phospho-ErbB2 and -ErbB3 with MUC4 by sequential immunoprecipitation and immunoblotting. Wounded cultures exhibited increased phosphorylation of both receptors in complex with MUC4. Scratch wounding also increased activation of the downstream pathway through Akt, as predicted from our previous studies on Muc4 effects on ErbB2 and ErbB3. The participation of MUC4 in the phosphorylation response was also indicated by siRNA repression of MUC4 expression, which resulted in diminution of the phosphorylation of ErbB2 and ErbB3. These studies provide a new model for the airway epithelial damage response, in which the MUC4-ErbB2 complex is a key element in the sensor mechanism and phosphorylation of the receptors.
- Published
- 2009
4. Molecular signaling in the regulation of mucins
- Author
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Kermit L. Carraway and George Theodoropoulos
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MAPK/ERK pathway ,Molecular signaling ,Erk signaling ,Mucin ,Mucins ,Cell Biology ,Biology ,Models, Biological ,Biochemistry ,Cell biology ,Gene Expression Regulation ,Animals ,Humans ,Signal transduction ,Protein Processing, Post-Translational ,Molecular Biology ,Transcription factor ,Erk map kinase ,MUC1 ,Signal Transduction ,Transcription Factors - Abstract
Mucins are large, highly glycosylated proteins involved in the protection of epithelia. The 20 different mucins show a diverse and highly regulated distribution among different epithelia. Most of the studies on mucin regulation done to date have been on the membrane mucins MUC1 and MUC4 and the gel-forming mucins. Multiple mechanisms have been implicated in that regulation, including examples at the transcriptional, transcript stabilization and post-translational levels. Several signaling pathways have been demonstrated to be involved, most frequently the canonical Erk MAP kinase pathway, but also the cytokine-JAK-STAT pathway and TGFbeta-SMAD pathways. Diversity in Erk signaling is achieved through multiple activation mechanisms and multiple downstream transcriptional factors that are affected. Given the still limited amount of information available on regulation of most of the mucins, other mechanisms and pathways are likely to be uncovered in the future.
- Published
- 2007
5. Expression, location, and interactions of ErbB2 and its intramembrane ligand Muc4 (sialomucin complex) in rat mammary gland during pregnancy
- Author
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Min Rong, Shari A. Price-Schiavi, Peter Li, Coralie A. Carothers Carraway, Nebila Idris, Eran R. Andrechek, Jin Zhang, Kermit L. Carraway, and William J. Muller
- Subjects
Genetically modified mouse ,medicine.medical_specialty ,Receptor, ErbB-2 ,Physiology ,Protein subunit ,Clinical Biochemistry ,Mammary gland ,Mammary Neoplasms, Animal ,Mice, Inbred Strains ,Mice, Transgenic ,Adenocarcinoma ,In Vitro Techniques ,Biology ,Ligands ,Alveolar cells ,Mice ,Mammary Glands, Animal ,Pregnancy ,Glycoprotein complex ,Cell Line, Tumor ,Internal medicine ,medicine ,Animals ,Lactation ,ERBB3 ,Glycoproteins ,Mucin-4 ,Mucins ,Epithelial Cells ,Cell Biology ,Ligand (biochemistry) ,Rats, Inbred F344 ,Transmembrane protein ,Rats ,Cell biology ,medicine.anatomical_structure ,Endocrinology ,Pregnancy, Animal ,Female ,sense organs - Abstract
Muc4 (also called Sialomucin complex) is a heterodimeric glycoprotein complex consisting of a peripheral O-glycosylated subunit ASGP-1 (ascites sialoglycoprotein-1) tightly but non-covalently bound to an N-glycosylated transmembrane subunit ASGP-2. Muc4/SMC can act as an intramembrane ligand for ErbB2 via an EGF-like domain present in the transmembrane subunit. The complex is developmentally regulated in normal rat mammary gland and overexpressed in a number of mammary tumors. Overexpression of Muc4/SMC has been shown to block cell–cell and cell–matrix interactions, protect tumor cells from immune surveillance, promote metastasis, and protect from apoptosis. We have investigated whether Muc4/SMC and ErbB2 are co-expressed and co-localized in normal rat mammary gland and whether Muc4/SMC–ErbB2 complex formation is developmentally regulated in this tissue. Muc4/SMC and ErbB2 have different expression patterns and regulatory mechanisms in the developing rat mammary gland, but both are maximally expressed during late pregnancy and lactation. The two proteins form a complex in lactating mammary gland which is not detected in the virgin gland. Moreover, this complex does not contain ErbB3. ErbB2 is co-localized with Muc4/SMC at the apical surfaces of ductal and alveolar cells in lactating gland; however, another form of ErbB2, recognized by a different antibody, localizes to the basolateral surfaces of these cells. ErbB2 phosphorylated on Tyr 1248 co-localized with Muc4/SMC at the apical surface but not at the basolateral surfaces of these cells. To investigate the function of Muc4 in the mammary gland, transgenic mice were derived using an MMTV-Muc4 construct. Interestingly, mammary gland development in the transgenic mice was aberrant, exhibiting a bifurcated pattern, including invasion down the blood vessel, similar to that exhibited by transgenic mice inappropriately expressing activated ErbB2 in the mammary gland. These data provide further evidence of the ability of Muc4/SMC to interact with ErbB2 and influence its behavior in normal epithelia. © 2004 Wiley-Liss, Inc.
- Published
- 2005
6. Presence of MUC4 in human milk and at the luminal surfaces of blood vessels
- Author
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Coralie A. Carothers Carraway, Pedro Soto, George Theodoropoulos, Min Rong, Mohammad Yasin, Jin Zhang, Kermit L. Carraway, and Aymee Perez
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Physiology ,medicine.drug_class ,Sialoglycoproteins ,Clinical Biochemistry ,Gene Expression ,Mammary Neoplasms, Animal ,Adenocarcinoma ,Biology ,Monoclonal antibody ,Umbilical vein ,Mice ,Antibody Specificity ,Cell Line, Tumor ,Chlorocebus aethiops ,medicine ,Animals ,Matrigel ,Hybridomas ,Milk, Human ,Mucin-4 ,Reverse Transcriptase Polymerase Chain Reaction ,Mucin ,Mucins ,Antibodies, Monoclonal ,Endothelial Cells ,Cell Biology ,Immunohistochemistry ,Molecular biology ,Epithelium ,Rats ,Endothelial stem cell ,medicine.anatomical_structure ,COS Cells ,Immunology ,biology.protein ,sense organs ,Antibody - Abstract
MUC4 is a heterodimeric membrane mucin, composed of a mucin subunit ASGP-1 (MUC4α) and a transmembrane subunit ASGP-2 (MUC4β), which has been implicated in the protection of epithelial cell surfaces. Surprisingly, development and characterization of a new monoclonal antibody (mAb), called 1G8, against ASGP-2 demonstrated by immunohistochemistry the presence of MUC4 at the luminal surfaces of blood vessels of both normal tissues and tumors. Muc4 was detected with 1G8 and other Muc4 antibodies in blood vessels from humans, rats and mice. This expression of MUC4 in endothelial cells was confirmed by immunoblotting with 1G8 in human umbilical vein endothelial cells (HUVECs), human iliac artery endothelial cells (HIAECs), and human microvascular endothelial cells (HMVECs). MUC4 could be observed on HUVECs grown on either plastic or Matrigel. Finally, MUC4 expression in the three types of endothelial cell lines was confirmed by reverse transcription-polymerase chain reaction (RT-PCR). These results provide, to our knowledge, the first demonstration of a member of the MUC gene family and membrane mucin in blood vessels. As a luminal surface component, the MUC4 is situated to contribute to the non-adhesive luminal surface and to act as an intrinsic protection and survival factor. © 2005 Wiley-Liss, Inc.
- Published
- 2005
7. MUC4 and ERBB2 expression in major and minor salivary gland mucoepidermoid carcinoma
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Francisco J. Civantos, Jose Ruiz, Carmen Gomez-Fernandez, Jeffrey Pacheco, Kara L. Hamilton-Nelson, W. Jarrard Goodwin, Mohammed Yasin, Donald T. Weed, David Arnold, Kermit L. Carraway, and Jin Zhang
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Adult ,Male ,medicine.medical_specialty ,Pathology ,Receptor, ErbB-2 ,Receptor expression ,Gastroenterology ,Antigen ,Antigens, Neoplasm ,Mucoepidermoid carcinoma ,Internal medicine ,Biomarkers, Tumor ,medicine ,Carcinoma ,Humans ,Registries ,Survival analysis ,Aged ,Retrospective Studies ,Mucin-4 ,business.industry ,Mucins ,Anatomical pathology ,Middle Aged ,Salivary Gland Neoplasms ,medicine.disease ,Survival Analysis ,Otorhinolaryngology ,Immunohistochemistry ,Carcinoma, Mucoepidermoid ,Female ,Salivary Gland Mucoepidermoid Carcinoma ,Neoplasm Recurrence, Local ,business - Abstract
Background. Peptide sequence homology between the gene product of human MUC4 and rat sialomucin complex (SMC) has recently been reported. Each contains a mucin subunit with antiadhesive activity linked to the plasma membrane by means of a transmembrane subunit with two epidermal growth factor (EGF)–like domains that act as ligand for ErbB2. This study investigates MUC4 and ErbB2 receptor expression in major and minor salivary gland mucoepidermoid carcinoma and correlates patterns of expression with clinical outcomes. Methods. MUC4 antigens and ErbB2 receptor expression are localized by immunohistochemical studies that use archival formalin-fixed and paraffin-embedded tissue. Clinical outcomes are determined by retrospective chart review of all patients (n = 28) with available archived pathologic specimens at the University of Miami–affiliated hospitals treated between 1994 and 2000. Results. Median survival time was 24 months (range, 2–60 months) among the nine patients who died, whereas median follow-up time in the remaining 19 patients is 33.4 months (range, 4.7–73 months). A trend toward a reduction in MUC4 antigen expression in high-grade tumors (55% expression) compared with low-grade (91% expression) and intermediate-grade (100% expression) tumors is identified (chi square, p = .0975). Patients with tumors expressing MUC4 antigens are at reduced risk of death (hazard ratio [HR], 0.20; p = .0531). Adjustment for pathologic grade, T stage, and age results in a much higher risk of death for patients whose tumors do not express MUC4 antigens, although this does not meet statistical significance (HR, 26.6; p = .1). Analysis of recurrence adjusting for T stage reveals that patients whose tumors do not express MUC4 antigens are at increased risk of recurrence compared with patients whose tumor expresses MUC4 antigens (HR, 6.37; p = .03). ErbB2 receptor staining is noted in seven of 28 patients, with five of these seven showing 2+ and 3+ membrane-staining patterns. Adjustment for pathologic grade and age suggests that patients whose tumors express high levels of ErbB2 (2+, 3+) are at increased risk of death compared with patients with low or no expression of ErbB2 (HR, 2.29; p = .32). MUC4 antigen positivity is seen in two of the five cases with 2+ and 3+ staining for ErbB2. Conclusions. These findings suggest MUC4 antigen positivity is associated with reduced risk of death and reduced risk of recurrence and may identify a subset of patients with more favorable prognosis. Although limited by small sample size, analysis reveals ErbB2 overexpression is not consistently associated with MUC4 antigen positivity and might be associated with increased risk of death © 2004 Wiley Periodicals, Inc. Head Neck26: 353–364, 2004
- Published
- 2004
8. Expression and localization of Muc4/sialomucin complex (SMC) in the adult and developing rat intestine: Implications for Muc4/SMC function
- Author
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Richard R. McNeer, Jin Zhang, Min Rong, Coralie A. Carothers Carraway, Kermit L. Carraway, Mohammad Yasin, Jan M.H. van den Brande, Donald T. Weed, Edmund A. Rossi, and John F. Thompson
- Subjects
Aging ,Paneth Cells ,Colon ,Duodenum ,Physiology ,Clinical Biochemistry ,Cell ,Biology ,Immunofluorescence ,Esophagus ,Species Specificity ,Intestine, Small ,medicine ,Animals ,Humans ,Intestinal Mucosa ,Gastrointestinal tract ,Mucin-4 ,medicine.diagnostic_test ,Secretory Vesicles ,Mucin ,Age Factors ,Mucins ,Cell Differentiation ,Cell Biology ,Anatomy ,Rats, Inbred F344 ,Epithelium ,Small intestine ,Cell Compartmentation ,Rats ,Cell biology ,medicine.anatomical_structure ,Immunohistochemistry ,Female ,Goblet Cells ,sense organs ,Intracellular - Abstract
Muc4/sialomucin complex (SMC) is a high molecular mass heterodimeric membrane mucin, encoded by a single gene, and originally discovered in a highly metastatic ascites rat mammary adenocarcinoma. Subsequent studies have shown that it is a prominent component of many accessible and vulnerable epithelia, including the gastrointestinal tract. Immunoblot and immunofluorescence analyses demonstrated that Muc4/SMC expression in the rat small intestine increases from proximal to distal regions and is located predominantly in cells at the base of the crypts. These cells were postulated to be Paneth cells, based on their location, morphology, and secretory granule content. Immunohistochemistry indicated the presence of Muc4/SMC in these granules. Muc4/SMC expression was higher in the rat colon than small intestine and was abundantly present in colonic goblet cells, but not in goblet cells in the small intestine. Immunohistochemistry also suggested the presence of MUC4 in human colonic goblet cells. Biochemical analyses indicated that rat colonic Muc4/SMC is primarily the soluble form of the membrane mucin. Analyses of Muc4/SMC during development of the rat gastrointestinal tract showed its appearance at embryonic day 14 of the esophagus and at day 15 at the surface of the undifferentiated stratified epithelium at the gastroduodenal junction, then later at cell surfaces in the more distal regions of the differentiated epithelium of the small intestine, culminating in expression as an intracellular form in the crypts of the small intestine at about day 21. Limited expression in the colon was observed during development before birth at cell surfaces, with expression as an intracellular form in the goblet cells arising during the second week after birth. These results suggest that membrane mucin Muc4/SMC serves different functions during development of the intestine in the rat, but is primarily a secreted product in the adult animal.
- Published
- 2004
9. Cell signaling through membrane mucins
- Author
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Coralie A. Carothers Carraway, Bushra Haq, Victoria P. Ramsauer, and Kermit L. Carraway
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Receptor complex ,Cell signaling ,Receptor, ErbB-2 ,Molecular Sequence Data ,Biology ,Ligands ,Models, Biological ,General Biochemistry, Genetics and Molecular Biology ,Receptor tyrosine kinase ,Adherens junction ,ErbB ,Animals ,Humans ,ERBB3 ,Amino Acid Sequence ,Phosphorylation ,skin and connective tissue diseases ,Autocrine signalling ,neoplasms ,Mucin-4 ,Cell Membrane ,Mucin-1 ,Mucins ,Cell biology ,biology.protein ,Protein Binding ,Signal Transduction - Abstract
MUC1 and MUC4 are the two membrane mucins that have been best characterized. Although they have superficially similar structures and have both been shown to provide steric protection of epithelial surfaces, recent studies have also implicated them in cellular signaling. They act by substantially different mechanisms, MUC4 as a receptor ligand and MUC1 as a docking protein for signaling molecules. MUC4 is a novel intramembrane ligand for the receptor tyrosine kinase ErbB2/HER2/Neu, triggering a specific phosphorylation of the ErbB2 in the absence of other ErbB ligands and potentiating phosphorylation and signaling through the ErbB2/ErbB3 heterodimeric receptor complex formed in the presence of neuregulin. In contrast, MUC1 has a highly conserved cytoplasmic tail, which binds β-catenin, a key component of adherens junctions and a regulator of transcription, in a process that is tightly regulated by MUC1 phosphorylation. The specific localization of these membrane mucins to the apical surfaces of epithelial cells suggests that their signaling functions may be important as sensor mechanisms in response to invasion or damage of epithelia. BioEssays 25:66–71, 2003. © 2002 Wiley Periodicals, Inc.
- Published
- 2002
10. Rat Muc4 (sialomucin complex) reduces binding of anti-ErbB2 antibodies to tumor cell surfaces, a potential mechanism for herceptin resistance
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Scott Jepson, Kermit L. Carraway, Shari A. Price-Schiavi, Lisa D. Yee, Philip S. Rudland, Peter Li, and Maria E Arango
- Subjects
Cancer Research ,medicine.medical_specialty ,Receptor, ErbB-2 ,medicine.drug_class ,Blotting, Western ,Antineoplastic Agents ,Breast Neoplasms ,Adenocarcinoma ,Biology ,Antibodies, Monoclonal, Humanized ,Transfection ,Monoclonal antibody ,Immunoenzyme Techniques ,Glycoprotein complex ,Internal medicine ,Tumor Cells, Cultured ,ERBB2 Antibody ,medicine ,Animals ,Humans ,skin and connective tissue diseases ,Receptor ,Melanoma ,neoplasms ,Mucin-4 ,Mucins ,Antibodies, Monoclonal ,Trastuzumab ,Ligand (biochemistry) ,Precipitin Tests ,Rats ,Cell biology ,Endocrinology ,Oncology ,Drug Resistance, Neoplasm ,Cell culture ,biology.protein ,Female ,sense organs ,Antibody - Abstract
Muc4 (also called sialomucin complex), the rat homolog of human MUC4, is a heterodimeric glycoprotein complex that consists of a peripheral O-glycosylated mucin subunit, ASGP-1, tightly but noncovalently linked to a N-glycosylated transmembrane subunit, ASGP-2. The complex is expressed in a number of normal, vulnerable epithelial tissues, including mammary gland, uterus, colon, cornea and trachea. Muc4/SMC is also overexpressed or aberrantly expressed on a number of human tumors including breast tumors. Overexpression of Muc4/SMC has been shown to block cell-cell and cell-matrix interactions, protect tumor cells from immune surveillance and promote metastasis. In addition, as a ligand for ErbB2, Muc4/SMC can potentiate phosphorylation of ErbB2 and potentially alter signals generated from this receptor. Using A375 human melanoma cells and MCF7 human breast adenocarcinoma cells stably transfected with tetracycline regulatable Muc4, we have investigated whether overexpression of Muc4/SMC can repress antibody binding to cell surface-expressed ErbB2. Overexpression of Muc4/SMC does not affect the level of ErbB2 expression in either cell line, but it does reduce binding of a number of anti-ErbB2 antibodies, including Herceptin. Interestingly, overexpression of ErbB2 does not block binding of other unrelated antibodies of the same isotype, suggesting that the reduction in ErbB2 antibody binding is due to complex formation of Muc4/SMC and ErbB2. Furthermore, capping of Muc4/SMC with anti-Muc4/SMC antibodies reduces antibody binding to ErbB2 instead of increasing binding, again suggesting that reduced antibody binding to ErbB2 is due to steric hindrance from complex formation of Muc4/SMC and ErbB2. Thus, overexpression of Muc4/SMC on tumor cells may have both prognostic and therapeutic relevance.
- Published
- 2002
11. MUC4 (Sialomucin Complex) Expression in Salivary Gland Tumors and Squamous Cell Carcinoma of the Upper Aerodigestive Tract
- Author
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W. Jarrard Goodwin, Thomas D. Lee, Esteban Bonfante, Kermit L. Carraway, Jeffrey Pacheco, Maria E. Carvajal, Donald T. Weed, and Carmen Gomez-Fernandez
- Subjects
Adult ,Male ,0301 basic medicine ,Pathology ,medicine.medical_specialty ,Blotting, Western ,Respiratory Mucosa ,Malignancy ,Salivary Glands ,03 medical and health sciences ,Antigen ,Biomarkers, Tumor ,medicine ,Humans ,Basal cell ,Aged ,Neoplasm Staging ,Aged, 80 and over ,Mucin-4 ,030102 biochemistry & molecular biology ,Salivary gland ,business.industry ,Mouth Mucosa ,Mucins ,Antibodies, Monoclonal ,Middle Aged ,Salivary Gland Neoplasms ,medicine.disease ,Immunohistochemistry ,Staining ,Parotid malignancy ,Otorhinolaryngologic Neoplasms ,stomatognathic diseases ,030104 developmental biology ,Upper aerodigestive tract ,medicine.anatomical_structure ,Otorhinolaryngology ,Cervical lymph nodes ,Carcinoma, Squamous Cell ,Female ,Surgery ,sense organs ,business - Abstract
This study investigates MUC4 expression in normal squamous epithelia and squamous cell carcinoma (SCC) of the upper aerodigestive tract (UADT), and in salivary gland neoplasms.MUC4 antigens in tumor and adjacent normal tissue are localized by immunocytochemical studies. Fresh frozen tissues from surgical resection specimens are further analyzed by Western blot.MUC4 is identified by immunocytochemical staining throughout the normal UADT mucosa, in 34 of 40 primary UADT SCC, and in 11 of 12 metastatic cervical lymph nodes. A trend toward decreased MUC4 staining in moderately and poorly differentiated tumors is noted. Immunoblots show MUC4 in 4 of 5 SCC analyzed. Immunocytochemical staining of MUC4 in 13 major and minor salivary gland neoplasms reveal variable staining of normal and neoplastic tissue. MUC4 is demonstrated in immunoblots of normal parotid tissue and in the single parotid malignancy analyzed, but is not demonstrated in one minor salivary gland malignancy. These findings characterize normal UADT mucosal and salivary MUC4 expression, and MUC4 expression in SCC of the UADT and in salivary gland tumors.Correlation of MUC4 expression with clinical outcomes may establish MUC4 as a potential molecular prognostic marker for these tumors.
- Published
- 2001
12. Sialomucin complex (rat Muc4) transmembrane subunit binds the differentiation marker peanut lectin in the normal rat mammary gland
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Peter Li, Philip S. Rudland, Shari A. Price-Schiavi, and Kermit L. Carraway
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chemistry.chemical_classification ,biology ,Physiology ,Immunoprecipitation ,Protein subunit ,Clinical Biochemistry ,Mucin ,Lectin ,Cell Biology ,Apical membrane ,Molecular biology ,Transmembrane protein ,Blot ,chemistry ,biology.protein ,sense organs ,Glycoprotein - Abstract
Sialomucin complex (SMC, rat Muc4) is a heterodimeric glycoprotein composed of two subunits, the mucin component ASGP-1 and the transmembrane subunit ASGP-2. SMC/Muc4 is highly expressed on the surface of 13762 rat mammary adenocarcinoma cells at approximately 100 times the level found in the lactating mammary gland. Immunocytochemical staining of SMC/Muc4 in the developing rat mammary gland is localized to the apical membrane of the ductal epithelium. This staining pattern is similar to that for peanut lectin, a differentiation marker, which binds to cells expressing the disaccharide Thomsen‐Friedenreich or TF antigen. Blotting of glycoproteins expressing the TF antigen from mammary tissues with peanut lectin detects a protein matching the migration of ASGP-2. Analysis of immunoprecipitated SMC/Muc4 by peanut lectin blotting shows that the TF antigen is abundantly present on the ASGP-2 subunit, hence the similarity of staining pattern with SMC/Muc4 antisera and peroxidase-conjugated lectin in mammary tissues. The TF antigen is also present on ASGP-2 of SMC/Muc4 produced by confluent cultures of Rama 37 rat mammary epithelial stem cells after their induction to an alveolar-like phenotype with prolactin. These results indicate that the TF antigen is present on the ASGP-2 transmembrane subunit of SMC/Muc4 from phenotypically normal tissues and cells, in contrast to malignant cells whose peanut lectin-binding disaccharide is located on ASGP-1. J. Cell. Physiol.
- Published
- 2001
13. Potentiation of metastasis by cell surface sialomucin complex (rat MUC4), a multifunctional anti-adhesive glycoprotein
- Author
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Lisa M. Tatum, Norman H. Altman, Kermit L. Carraway, Coralie A. Carothers Carraway, and Masanobu Komatsu
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Cancer Research ,Pathology ,medicine.medical_specialty ,Melanoma ,Mucin ,Cell ,Transfection ,Biology ,musculoskeletal system ,medicine.disease ,Metastasis ,medicine.anatomical_structure ,Oncology ,Downregulation and upregulation ,In vivo ,cardiovascular system ,Cancer research ,medicine ,Cytotoxic T cell ,tissues - Abstract
Sialomucin complex (SMC), a rat homologue of the human mucin MUC4, is a large membrane-bound mucin complex, originally isolated from highly metastatic ascites 13762 mammary adenocarcinoma cells. When overexpressed, SMC exerts potent anti-adhesive effects, which sterically disrupt molecular interactions for cell-cell and cell-ECM adhesions. SMC similarly suppresses anti-tumor immunity by inhibition of interactions between cytotoxic lymphocytes and target tumor cells. Previously, recombinant cDNAs for SMC were transfected and inducibly expressed in A375 human melanoma cells using a tetracycline-responsive expression system. In the current studies, we investigated the role of MUC4/SMC in tumor metastasis by regulating SMC expression of tumor transplants in vivo. Intravenous injection of SMC-overexpressing cells resulted in substantially greater lung metastasis than injection of SMC-repressed cells. Injection of SMC-overexpressing cells followed by in vivo downregulation of SMC did not lower the frequency of lung metastasis. Growth of the micrometastatic lesions was the same for all 3 cases in short-term (3-week) assays. Further, subcutaneous injection of A375 cells followed by in vivo induction of SMC overexpression within the solid tumor resulted in spontaneous distant metastasis. These studies suggest that SMC potentiates metastasis by contributing to the establishment of metastatic foci. These studies directly demonstrate for the first time that tumor metastasis can be modulated by the regulation of MUC4/SMC expression. Int. J. Cancer 87:480–486, 2000. © 2000 Wiley-Liss, Inc.
- Published
- 2000
14. Characterization of the expression and steroid hormone control of sialomucin complex in the rat uterus: Implications for uterine receptivity
- Author
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Coralie A. Carothers Carraway, Richard R. McNeer, Nevis Fregien, and Kermit L. Carraway
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medicine.medical_specialty ,Physiology ,medicine.medical_treatment ,Clinical Biochemistry ,Mucin ,Mammary gland ,Uterus ,Cell Biology ,Biology ,Endometrium ,Transmembrane protein ,Cell biology ,Steroid hormone ,medicine.anatomical_structure ,Endocrinology ,Downregulation and upregulation ,Glycoprotein complex ,Internal medicine ,medicine - Abstract
The sialomucin complex (SMC), originally isolated as a heterodimeric glycoprotein complex from membranes of ascites sublines of a highly metastatic mammary adenocarcinoma, consists of a high Mr mucin subunit (ASGP-1, ascites sialoglycoprotein) and a transmembrane subunit (ASGP-2) with two epidermal growth factor-like domains. SMC has been characterized in the mammary gland, where it is present in both membrane and nonmembrane (soluble) forms, the latter lacking its transmembrane domain. SMC in the mammary gland is observed during pregnancy and lactation, but not in the virgin gland, and is regulated by a posttranscriptional mechanism. Both membrane and nonmembrane forms of SMC are found in rat uterus, also as a complex of ASGP-1 and ASGP-2. Immunocytochemical analyses indicate that the primary site of expression is at the luminal surface of the endometrium. Approximately 40% of the SMC, corresponding to the nonmembrane fraction, is removed by rinsing uterine preparations with saline, indicating that the soluble form is adsorbed loosely to the cell surface. In contrast to mammary gland, SMC is most highly expressed in the virgin animal, and its expression varies during the estrous cycle with the steady state level of transcript. The complex is present in a location consistent with steric inhibition of blastocyst implantation and is abruptly lost at the beginning of the period of receptivity for implantation. Expression of SMC in the uterus is regulated by estrogen and progesterone and is inversely correlated with receptivity. Both implantation and loss of SMC expression can be blocked with RU486. We propose that the downregulation of SMC and its loss from the apical surface of the rat uterine lining contribute to the generation of the receptive state for uterine implantation. Furthermore, the presence of both membrane and soluble SMC at the luminal surface of the endometrium may provide a model for its proposed protective function in other accessible and vulnerable epithelia. J. Cell. Physiol. 176:110–119, 1998. © 1998 Wiley-Liss, Inc.
- Published
- 1998
15. Signaling, mitogenesis and the cytoskeleton: Where the action is
- Author
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Coralie A. Carothers Carraway and Kermit L. Carraway
- Subjects
MAPK/ERK pathway ,Epidermal Growth Factor ,biology ,GRB7 ,JAK-STAT signaling pathway ,General Biochemistry, Genetics and Molecular Biology ,SH3 domain ,Receptor tyrosine kinase ,Cell biology ,Biochemistry ,GTP-Binding Proteins ,Mitogen-activated protein kinase ,biology.protein ,Animals ,Humans ,NCK1 ,c-Raf ,Cell Division ,Cytoskeleton ,Signal Transduction - Abstract
Stimulation of mitogenesis by the epidermal growth factor (EGF) operates through a pathway involving the receptor, the small G-protein Ras and protein kinases of the MAP kinase cascade. It is proposed that two of the critical steps of that pathway utilize localization of components to the plasma membrane where Ras is located: recruitment of the nucleotide exchange protein Sos to the phosphorylated EGF receptor via a complex with the SH2/SH3-containing protein Grb2 and recruitment of the protein kinase Raf to activated Ras. Moreover, it is then proposed that Raf associates with the cytoskeleton at the membrane as it is being activated. Other signaling elements, including class I receptor kinases, nonreceptor tyrosine kinases and tyrosine phosphatases, are known to function at specific cellular sites. These observations have led us to propose that localization of signaling components, and particularly sites at membrane-microfilament interfaces, play critical roles in cellular regulation.
- Published
- 1995
16. Sialomucin Complex (Muc4) Expression in Porcine Endometrium During the Oestrous Cycle and Early Pregnancy
- Author
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Rodney D. Geisert, A D Ferrell, Jerry R. Malayer, and Kermit L. Carraway
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medicine.medical_specialty ,Swine ,Uterus ,Estrous Cycle ,Biology ,Endometrium ,Glycocalyx ,Endocrinology ,Pregnancy ,Internal medicine ,medicine ,Animals ,Conceptus ,reproductive and urinary physiology ,Estrous cycle ,Mucin-4 ,Mucins ,medicine.disease ,Immunohistochemistry ,medicine.anatomical_structure ,In utero ,Pregnancy, Animal ,Female ,Animal Science and Zoology ,sense organs ,Immunostaining ,Biotechnology - Abstract
The non-invasive type of implantation in the pig is characterized by the maintenance of a thick glycocalyx coating on the uterine epithelial surface microvilli. Present study investigated the alteration in the sialomucin complex (Muc4) expression during the oestrous cycle and early pregnancy in the pig. Endometrial tissue samples were immunostained with the primary antibody to the Muc4 transmembrane subunit ASGP-2. Muc4 immunostaining increased in the surface and glandular epithelia between days 5 and 10 of oestrous cycle. Immunostaining continued to increase on day 12 with the greatest intensity of uterine Muc4 immunostaining detected on day 15 of the oestrous cycle and early pregnancy. Endometrial Muc4 expression in cyclic gilts decreased dramatically during early proestrous but continued to remain abundant in the surface and glandular epithelium of pregnant gilts during the period of conceptus attachment to the uterine surface.
- Published
- 2003
17. Membrane Mucin Muc4 Induces Density Dependent Changes in Erk Activation in Mammary Epithelial and Tumor Cells
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Vanessa Pino, Coralie A. Carothers Carraway, Pedro J.I. Salas, and Kermit L. Carraway
- Subjects
MAPK/ERK pathway ,Membrane ,Density dependent ,Chemistry ,Mucin ,Genetics ,Tumor cells ,Molecular Biology ,Biochemistry ,Biotechnology ,Cell biology - Published
- 2006
18. MUC4(sialomucin complex) expression in salivary tumors and SCC of the upper aerodigestive tract
- Author
-
Jeffrey Pacheco, Thomas K. Lee, Adam Bello, W. Jarrard Goodwin, Donald T. Weed, Kermit L. Carraway, Carmen Gomez-Fernandez, and Mba Maria Carvajal
- Subjects
Pathology ,medicine.medical_specialty ,Upper aerodigestive tract ,Otorhinolaryngology ,business.industry ,Medicine ,Surgery ,business - Published
- 1999
19. What the papers say: Membranes and microfilaments: Interactions and role in cellular dynamics
- Author
-
Kermit L. Carraway
- Subjects
Membrane ,Chemistry ,Biophysics ,Cellular dynamics ,Microfilament ,General Biochemistry, Genetics and Molecular Biology - Published
- 1990
20. MUC4 and p27kip Expression in Squamous Cell Carcinoma of the Head and Neck
- Author
-
Michael J. Rodriguez, Carmen Gomez, Donald T. Weed, Joyce Slingerland, Mohhamad Yasin, and Kermit L. Carraway
- Subjects
Otorhinolaryngology ,Expression (architecture) ,business.industry ,Cancer research ,Medicine ,Surgery ,Basal cell ,business ,Head and neck - Published
- 2005
21. Letter to the editor
- Author
-
Kermit L. Carraway
- Subjects
Medical education ,Histology ,business.industry ,Medicine ,General Medicine ,Mythology ,Anatomy ,business ,Anatomy education - Published
- 1997
22. Phenothiazine binding by a homolog of calpactin, the pp60 src tyrosine kinase substrate
- Author
-
Kermit L. Carraway Iii, Yuecheung Liu, David Puett, Kermit L. Carraway, and Coralie A. Carothers Carraway
- Subjects
animal structures ,Calmodulin ,Chlorpromazine ,viruses ,chemistry.chemical_element ,Ether ,Calcium ,Microfilament ,Biochemistry ,Cell Line ,chemistry.chemical_compound ,Phenothiazines ,Phenothiazine ,Genetics ,Animals ,Molecular Biology ,Microvilli ,biology ,Microfilament Proteins ,fungi ,Mammary Neoplasms, Experimental ,Substrate (chemistry) ,Cooperative binding ,Neoplasm Proteins ,Molecular Weight ,chemistry ,embryonic structures ,Immunologic Techniques ,biology.protein ,Electrophoresis, Polyacrylamide Gel ,Tyrosine kinase ,Protein Binding ,Biotechnology - Abstract
Microvilli isolated from 13762 mammary ascites tumor cells contain a major calcium-sensitive protein (AMV-p35) that can be isolated with microvillar microfilament cores prepared by Triton X-100 extraction in the presence but not absence of calcium. AMV-p35 can be readily purified from ethylene glycol bis(beta-aminoethyl ether)-N,N,N',N'-tetraacetic acid extracts of the microfilament cores by chromatography on an anion exchange column, to which it does not bind. Immunoblot analysis indicates that AMV-p35 is related to calpactin I, the pp60src tyrosine kinase substrate. In the presence of calcium, AMV-p35 binds approximately 4 mol of chlorpromazine per mole of protein in a binding process showing apparent positive cooperativity, similar to calmodulin; however, in contrast to calmodulin, AMV-p35 also binds phenothiazine in the absence of calcium.
- Published
- 1987
23. Association of calmodulin and smooth muscle myosin light chain kinase: Application of a lable selection technique with trace acetylated calmodulin
- Author
-
Arthur E. Jackson, Kermit L. Carraway, Anthony R. Means, Keith Brew, M. E. Payne, and David Puett
- Subjects
chemistry.chemical_classification ,Binding Sites ,Myosin light-chain kinase ,Calmodulin ,biology ,Lysine ,Acetylation ,Muscle, Smooth ,In Vitro Techniques ,Binding, Competitive ,Biochemistry ,Enzyme ,chemistry ,Structural Biology ,biology.protein ,Animals ,Binding site ,Protein kinase A ,Myosin-Light-Chain Kinase ,Molecular Biology ,Macromolecule - Abstract
A method is described for rapidly surveying the effects of modifying individual amino acid residues of a protein on its ability to interact specifically with another macromolecule. The procedure has been used to examine the individual roles of the seven lysyl residues of calmodulin in its ability to bind to smooth muscle myosin light chain kinase; previous studies by Jackson et al. (J. Biol. Chem. 261:1226-12232, 1986) have suggested that certain lysines may be located close to the interaction site. Trace [3H]-acetylated calmodulin, consisting predominantly of molecules acetylated at single sites together with unmodified protein, was incubated in excess (five- to 20-fold) with smooth muscle MLC kinase to allow the modified and unmodified molecules to compete for binding to the enzyme. Subsequently, the calmodulin-enzyme complex was separated from unbound calmodulin, and the level of acetylation of each of the seven lysines of the bound fraction of calmodulin was determined and compared to that of each corresponding group of the starting preparation. Significant changes were found at only two of the lysines, 21 and 75, where the extent of acetylation in the bound fraction was three- and fivefold lower, respectively, than that in the original preparation. These results were reproducible in three separate selection experiments employing both chicken and turkey gizzard MLC kinase. It is concluded that acetylation of calmodulin at either lysine 21 or 75 markedly reduces its affinity for MLC kinase, but acetylation at any of the other lysines (13, 30, 77, 94, or 148) has only minor effects.(ABSTRACT TRUNCATED AT 250 WORDS)
- Published
- 1987
24. O-glycosylation pathway for mucin-type glycoproteins
- Author
-
Kermit L. Carraway and Steven R. Hull
- Subjects
chemistry.chemical_classification ,Cell type ,Glycosylation ,Cellular differentiation ,Mucins ,Oligosaccharides ,Cell Differentiation ,Biology ,General Biochemistry, Genetics and Molecular Biology ,Amino acid ,Serine ,chemistry.chemical_compound ,chemistry ,Biochemistry ,Neoplasms ,Animals ,Humans ,Threonine ,Glycoprotein ,Protein Processing, Post-Translational ,Cellular localization - Abstract
O-glycosylation is the post-translational process whereby carbohydrate is added to hydroxylated amino acids of proteins. The major O-glycosylation pathway in animal cells is involved in the synthesis of oligosaccharides linked by N-acetylgalactosamine to serine or threonine residues in 'mucin-type' proteins or their analogs. In this review, we discuss the evidence for the cellular localization of the biosynthetic steps in this pathway and propose a simplified, consensus version. We also propose variations of the simple pathway to account for its heterogeneity and variability in different cell types and differentiation states.
- Published
- 1989
25. Organizational forms of actin in 13762 ascites mammary tumor cell microvilli
- Author
-
Goeh Jung, Kermit L. Carraway, and Coralie A. Carothers Carraway
- Subjects
Octoxynol ,Arp2/3 complex ,macromolecular substances ,Microfilament ,Polyethylene Glycols ,Cell Movement ,Cell surface receptor ,medicine ,Animals ,Actin-binding protein ,Cytoskeleton ,Actin ,Glycoproteins ,chemistry.chemical_classification ,Microvilli ,biology ,Cell Membrane ,Mammary Neoplasms, Experimental ,Cell Biology ,Microvillus ,Actins ,Transmembrane protein ,Cell biology ,medicine.anatomical_structure ,chemistry ,biology.protein ,Glycoprotein - Abstract
The organization of microvillus actin and its associated proteins have been investigated in sublines of mammary ascites tumors (MAT) with mobile (MAT-B1) and immobile (MAT-C1) cell surface receptors. Microvilli isolated from these sublines differ in morphology (branched for MAT-C1 versus unbranched for MAT-B1) and the presence of a 58,000-dalton polypeptide (58K). 58K is found associated with MAT-C1 microvilli, microvillar cytoskeletons obtained by nonionic detergent extractions, and microvillar membranes prepared under conditions which depolymerize actin microfilaments. By extraction with actin-stabilizing buffers (isotonic Triton-Mg-ATP) microvillar actin can be fractionated into four forms. About 40% of the actin is sedimented at low speed (7,500g, 15 min). The pellets contain microfilaments; actin and alpha-actinin are the predominant proteins. High-speed pellets from these low-speed supernates contain about 10% of the actin as a transmembrane complex with a cell surface glycoprotein (cytoskeleton-associated glycoprotein, [CAG] 75-80,000 daltons) in MAT-B1 cells or with CAG and 58K in MAT-C1 cells. Transmembrane complexes can be purified from MAT-B1 and MAT-C1 microvillar membranes in Triton-containing buffer by gel filtration or sucrose density gradient centrifugation. The presence of only CAG and actin in the MAT-B1 transmembrane complex strongly suggests the direct interaction of actin and a cell surface component. The high-speed supernates contain soluble actin. By gel filtration or rate-zonal sucrose density gradient centrifugation about 30% of the microvillar actin is found as small oligomers and about 10% as G-actin in this extraction buffer. We suggest that the actin-containing transmembrane complexes may serve as membrane-association sites for oligomeric actin segments and microfilaments and as initiation sites for actin polymerization.
- Published
- 1983
26. Actin-associated cell-surface glycoprotein from ascites cell microvilli: A disulfide-linked multimer
- Author
-
Kermit L. Carraway, David M. Andrews, Coralie A. Carothers Carraway, and Goeh Jung
- Subjects
Protein Denaturation ,congenital, hereditary, and neonatal diseases and abnormalities ,Chemical Phenomena ,Macromolecular Substances ,Detergents ,Biology ,Microfilament ,Biochemistry ,Microvillus membrane ,chemistry.chemical_compound ,Centrifugation, Density Gradient ,medicine ,Animals ,Disulfides ,Sodium dodecyl sulfate ,Cytoskeleton ,Molecular Biology ,Glycoproteins ,Differential centrifugation ,Binding Sites ,Microvilli ,Ascites ,Mammary Neoplasms, Experimental ,Membrane Proteins ,Cell Biology ,Microvillus ,Molecular biology ,Actins ,Transmembrane protein ,Neoplasm Proteins ,Rats ,Sedimentation coefficient ,Chemistry ,Cytoskeletal Proteins ,medicine.anatomical_structure ,Solubility ,chemistry ,Female ,Oxidation-Reduction - Abstract
Isolated microvilli of the MAT-C1 subline of the 13762 rat mammary adenocarcinoma contain a transmembrane complex composed of a cell surface, cytoskeleton-associated glycoprotein (CAG), actin, and a 58,000-dalton polypeptide (58K). The behavior of CAG has been studied by differential centrifugation and velocity sedimentation gradient centrifugation of detergent extracts of microvilli. CAG can be pelleted along with a fraction of the microvillar actin even in the presence of ionic detergents and under microfilament-depolymerizing conditions. By velocity sedimentation analysis CAG in Triton/PBS extracts sediments as a large, heterogeneous species (sedimentation coefficient greater than 25S). In Sarkosyl and sodium dodecyl sulfate (SDS) the size and heterogeneity are somewhat reduced. In SDS CAG sediments as a 20S species in the absence of mercaptoethanol and as a 5S species in the presence of mercaptoethanol. These results indicate that CAG is a disulfide-linked multimer in the microvillus membrane. We suggest that the stable multimeric structure of CAG permits it to act as the membrane association site for several microfilaments and plays an important role in the formation and stabilization of the microvillus structure.
- Published
- 1985
27. Concanavalin a perturbation of membrane enzymes of mammary gland
- Author
-
Coralie A. Carothers Carraway and Kermit L. Carraway
- Subjects
ATPase ,Detergents ,Methylmannosides ,Polyethylene Glycols ,Mammary Glands, Animal ,Nucleotidases ,Nucleotidase ,Animals ,Receptor ,Adenosine Triphosphatases ,Galactosyltransferase ,chemistry.chemical_classification ,biology ,Cell Membrane ,Lectin ,General Medicine ,Alkaline Phosphatase ,Galactosyltransferases ,Molecular biology ,Rats ,Enzyme ,Biochemistry ,chemistry ,Concanavalin A ,Depression, Chemical ,biology.protein ,Alkaline phosphatase ,Female ,Deoxycholic Acid - Abstract
The plant lectin concanavalin A (Con A) specifically inactivates the 5'-nucleotidase of a plasma membrane-enriched fraction from lactating mammary gland. The lectin also causes an activation of the membrane Mg++-ATPase, but does not affect galactosyltransferase or alkaline phosphatase. The enzyme perturbations are prevented by alpha-methylmannoside, an inhibitor of Con A binding, indicating that specific binding to carbohydrate structures rather than nonspecific protein-protein interaction is involved. Solubilization of the 5'-nucleotidase in detergents (0.2% Triton X-100 or 1% deoxycholate) does not prevent Con A inactivation, indicating that incorporation into the membrane structure is not a requirement for the Con A effect. the results suggest that Con A inactivates the 5'-nucleotidase by a direct interaction with the enzyme and that this enzyme is a Con A receptor site on the surface of mammary cells.
- Published
- 1976
28. Characterization of Nx,Ny-Disubstituted adenines by ultraviolet absorption spectra
- Author
-
Kermit L. Carraway, Nelson J. Leonard, and John P. Helgeson
- Subjects
Chemistry ,Organic Chemistry ,Ultraviolet absorption ,Photochemistry ,Spectral line ,Characterization (materials science) - Abstract
By the synthesis of representative Nx,Ny-dibenzyladenines and determination of their ultraviolet absorption spectra in acidic, basic and neutral solution we have assembled a body of spectral characteristics which makes it possible to distinguish among possible N,N-disubstituted adenines.
- Published
- 1965
29. 5-Amino-5-deoxyribose derivatives. Synthesis and use in the preparation of 'Reversed' Nucleosides
- Author
-
Nelson J. Leonard and Kermit L. Carraway
- Subjects
chemistry.chemical_compound ,chemistry ,Deoxyribose ,Sodium ,Organic Chemistry ,Liquid ammonia ,medicine ,Organic chemistry ,chemistry.chemical_element ,Kinetin ,Nuclear magnetic resonance spectroscopy ,medicine.disease_cause ,Ultraviolet - Abstract
Methyl 5-amino-5-deoxy-2,3-O-isopropylidene-β-D-ribofuranoside (III) has been synthesized and used as an intermediate in the preparation of the kinetin analog, methyl 5-deoxy-5-(purin-6-yl)amino-β-D-ribofuranoside (X). The related 1-substituted adenine, methyl 5- (6-aminopurin-1-yl)-5-deoxy-2,3-O-isopropylidene-β-D-ribofuranoside (XIII), was prepared by cyclization of 1-benzyl-5-cyano-4-ethoxymethyleneaminoimidazole (XI) with III and subsequent debenzylation with sodium in liquid ammonia. The structures and stereochemistry of these compounds were established by a combination of ultraviolet and nuclear magnetic resonance spectroscopy.
- Published
- 1966
30. Phenothiazine binding by a homolog of calpactin, the pp60 src tyrosine kinase substrate
- Author
-
Iii, Kermit L. Carraway, primary, Liu, Yuecheung, additional, Puett, David, additional, Carraway, Kermit L., additional, and Carraway, Coralie A. Carothers, additional
- Published
- 1987
- Full Text
- View/download PDF
31. The cytoskeleton: Past, present and future
- Author
-
Melanie M. Pratt, Kermit L. Carraway, and David R. Burgess
- Subjects
Animals ,Biology ,Cytoskeleton ,Neuroscience ,General Biochemistry, Genetics and Molecular Biology - Published
- 1987
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