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Tissue engineering has been able to develop novel decellularization-recellularization techniques, which facilitates the research for the generation of functional organs. This is based in the initial obtention of the organ's extracellular matrix (ECM). Therefore, any improvement in the decellularization process would have a positive impact in the results of the recellularization process. Nevertheless, commonly the methods and equipment employed for this process are expensive and thus limit the access of this technique to various research groups globally. To develop a decellularization technique with the exclusive use of hydrostatic pressure of detergent solutions, to have an easily accessible and low-cost technique that meets the basic requirements of acellularity and functionality of the ECM. This experimental study was performed in 10 male Wistar rats, obtaining the liver to carry out serial washes, with 1%, 2%, and 3% Triton X-100 solutions and 0.1% SDS. The washes were performed by using a gravity perfusion system (GPS), which assured us a continuous hydrostatic pressure of 7.5 mmHg. The obtained ECM was processed using stains and immunostaining to determine the residual cell content and preservation of its components. The staining showed a removal of cellular and nuclear components of approximately 97% of the acellular ECM, with an adequate three-dimensional pattern of collagen and proteoglycans. Furthermore, the acellular ECM allowed the viability of a primary hepatocyte culture. The use of the GPS decellularization technique allowed us to obtain an acellular and functional ECM, drastically reducing experimentation costs.

The presence of FTO (rs9939609) and MC4R (rs17782313) single-nucleotide polymorphisms (SNPs) has been associated with an increased risk of overweight and obesity and alterations in the lipids profile. The aim of this study was to investigate the relation between these FTO and MC4R SNPs with the presence of overweight/obesity and metabolic risk in children. A total of 167 school-aged children (8 ± 1.5 years) from a rural area of ​​Queretaro, Mexico, have been evaluated until now. Weight, height, waist circumference and body composition (DXA) were measured. A fasting blood sample was taken for the analysis of glucose and lipid profile. Genotypic frequency of the SNPs was determined using qPCR. Prevalence of obesity and overweight was 12% and 17%, respectively. FTO genotypic frequency for TT, TA and AA was77%, 22% and 1%; the frequency of T and A alleles was 87% and 13%, respectively. As for MC4R, genotypic frequency for TT, TC and CC was 86.3%, 13% and 0.7%; the frequency of T and C alleles was ...