1. Inbred C57BL/6J and DBA/2J Mouse Strains Exhibit Constitutive Differences in Regional Brain Fatty Acid Composition
- Author
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Patrick Tso, Therese Rider, Robert K. McNamara, Ronald J. Jandacek, and Jessica A. Able
- Subjects
medicine.medical_specialty ,Clinical chemistry ,Hippocampus ,Biology ,Biochemistry ,Midbrain ,Mice ,chemistry.chemical_compound ,Internal medicine ,medicine ,Animals ,Brain Chemistry ,Fatty Acids ,Organic Chemistry ,Ventral striatum ,Brain ,food and beverages ,Cell Biology ,Mice, Inbred C57BL ,Oleic acid ,medicine.anatomical_structure ,Endocrinology ,chemistry ,Mice, Inbred DBA ,Docosahexaenoic acid ,lipids (amino acids, peptides, and proteins) ,Arachidonic acid ,Stearic acid - Abstract
Major behavioral and neurochemical features observed between inbred C57BL/6 and DBA/2 mouse strains can be reproduced within rodent strains following dietary-induced reductions in brain docosahexaenoic acid (DHA, 22:6n-3) composition. It was therefore hypothesized that C57BL/6 and DBA/2 mice exhibit constitutive differences in brain DHA composition that are independent of diet. To test this, adult C57BL/6J and DBA/2J prefrontal cortex, hippocampus, ventral striatum, and midbrain fatty acid composition was determined by gas chromatography. After correction for multiple comparisons, C57BL/6J mice exhibited significantly lower DHA composition in the hippocampus and ventral striatum, but not prefrontal cortex or midbrain, and significantly greater regional arachidonic acid (ARA, 20:4n-6):DHA ratios, relative to DBA/2J mice. C57BL/6J mice also exhibited significantly lower regional adrenic acid (ADA, 22:4n-6) composition, and a significantly smaller ADA:ARA ratio, relative to DBA/2J mice. C57BL/6J mice exhibited significantly smaller oleic acid:stearic acid ratio in the hippocampus and ventral striatum relative to DBA/2J mice. Among all mice, DHA composition was positively correlated with the ADA:ARA ratio and inversely correlated with the oleic acid:stearic acid ratio. These data demonstrate that inbred C57BL/6J and DBA/2J mouse strains exhibit constitutive and region-specific differences in fatty acid composition independent of diet, and suggest that heritable genetic factors are an important determinant of central fatty acid composition.
- Published
- 2008
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