Search

Single cell network profiling (SCNP) is a multi-parameter flow cytometry technique for simultaneous interrogation of intracellular signalling pathways. Diagnostic paediatric acute myeloid leukaemia (AML) bone marrow samples were used to develop a classifier for response to induction therapy in 53 samples and validated in an independent set of 68 samples. The area under the curve of a receiver operating characteristic curve (AUC(ROC)) was calculated to be 0·85 in the training set and after exclusion of induction deaths, the AUC(ROC) of the classifier was 0·70 (P = 0·02) and 0·67 (P = 0·04) in the validation set when induction deaths (intent to treat) were included. The highest predictive accuracy was noted in the cytogenetic intermediate risk patients (AUC(ROC) 0·88, P = 0·002), a subgroup that lacks prognostic/predictive biomarkers for induction response. Only white blood cell count and cytogenetic risk were associated with response to induction therapy in the validation set. After controlling for these variables, the SCNP classifier score was associated with complete remission (P = 0·017), indicating that the classifier provides information independent of other clinical variables that were jointly associated with response. This is the first validation of an SCNP classifier to predict response to induction chemotherapy. Herein we demonstrate the usefulness of quantitative SCNP under modulated conditions to provide independent information on AML disease biology and induction response.

Allowing selected full-thickness skin defects to heal by secondary intention offers the advantages of optimal cancer surveillance, simplified wound management, and avoidance of reconstructive procedures with their associated costs and potential complications. The topical use of bovine collagen has been suggested as a method of enhancing wound closure and final cosmetic appearance. This study evaluated the effect of bovine collagen on wound healing in patients undergoing facial Mohs surgery using the fresh-tissue technique. A total of 111 consecutive patients were assigned to a collagen or no-collagen group. Wound care was identical except for the weekly addition of bovine collagen to the wound of patients in the collagen group. Evaluation was at weekly intervals until the wound epithelialized, then bimonthly for at least 6 months. There was no difference in the rate of wound epithelialization or final cosmetic appearance. This study provides no evidence that the topical use of bovine collagen in a facial wound after Mohs surgery enhances wound epithelialization or the final cosmetic appearance.