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Your search keyword '"Jaime Andrés Rivas-Pardo"' showing total 2 results
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2 results on '"Jaime Andrés Rivas-Pardo"'

1. Mechanical Deformation Accelerates Protein Ageing

Author

Jessica Valle‐Orero, Jaime Andrés Rivas‐Pardo, Rafael Tapia‐Rojo, Ionel Popa, Daniel J. Echelman, Shubhasis Haldar, and Julio M. Fernández

Subjects
0301 basic medicine, 03 medical and health sciences, 030104 developmental biology, General Medicine, 010402 general chemistry, 01 natural sciences, 0104 chemical sciences
Published
2017

2. Dissecting the functional roles of the conserved NXXE and HXE motifs of the ADP-dependent glucokinase fromThermococcus litoralis

Author

Victoria Guixé, Jaime Andrés Rivas-Pardo, César A. Ramírez-Sarmiento, and María José Abarca-Lagunas

Subjects
Models, Molecular, Archaeal Proteins, Amino Acid Motifs, Biophysics, Protein–ligand binding, Biochemistry, Conserved sequence, chemistry.chemical_compound, Structural Biology, Catalytic Domain, Glucokinase, Genetics, Thermococcus litoralis, Molecular Biology, Ternary complex, Conserved Sequence, Divalent metal cation, biology, Archaeal enzyme, Cell Biology, biology.organism_classification, Thermococcus, Glucose binding, Kinetics, Glucose, Glucose 6-phosphate, chemistry, ADP-dependent kinase, Mutagenesis, Site-Directed, Adenosine triphosphate
Abstract

The activity of the ADP-dependent glucokinase from Thermococcus litoralis (TlGK) relies on the highly conserved motifs NXXE (i.e. Asn-Xaa-Xaa-Glu) and HXE (i.e. His-Xaa-Glu). Site-directed mutagenesis of residues Glu279 (HXE) and Glu308 (NXXE) leads to enzymes with highly reduced catalytic rates. The replacement of Glu308 by Gln increased the KM for MgADP(-) and was activated by free Mg(2+). On the other hand, HXE mutants did not affect the KM for MgADP(-), were still inhibited by free Mg(2+), and caused a large increase on KM for glucose and an 87-fold weaker binding of glucose onto the non-hydrolysable TlGK·AMP-AlF3 complex. Our findings put forward the fundamental role of the HXE motif in glucose binding during ternary complex formation.

Published
2015

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