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Your search keyword '"Iijima, Hirotaka"' showing total 15 results
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15 results on '"Iijima, Hirotaka"'

7. Culture temperature affects redifferentiation and cartilaginous extracellular matrix formation in dedifferentiated human chondrocytes

Author

Ito, Akira, Aoyama, Tomoki, Iijima, Hirotaka, Tajino, Junichi, Nagai, Momoko, Yamaguchi, Shoki, Zhang, Xiangkai, and Kuroki, Hiroshi

Subjects
extracellular matrix, chondrocyte, temperature, pellet culture, differentiation
Abstract

To date, there have been few studies on how temperature affects the phenotype and metabolism of human chondrocytes. Thus, the purpose of this study was to elucidate the effects of culture temperature on chondrocyte redifferentiation and extracellular matrix (ECM) formation using dedifferentiated mature human chondrocytes in vitro. Dedifferentiated chondrocytes were cultured in a pellet culture system for up to 21 days. The pellets were randomly divided into three groups with different culture temperature (32, 37, and 41°C). Chondrocyte redifferentiation and ECM formation were evaluated by wet weight, messenger ribonucleic acid (mRNA), histological, and biochemical analyses. The results showed that the wet weight and the mRNA expressions of collagen type II A1 and cartilage oligomeric matrix protein at 37°C were higher than the corresponding values at 32°C. The histological and biochemical analyses revealed that the syntheses of type II collagen and proteoglycan were promoted at 37°C compared to those at 32°C, whereas they were considerably inhibited at 41°C. In conclusion, the results obtained herein indicated that temperature affects chondrocyte redifferentiation and ECM formation, and modulation of temperature might thus represent an advantageous means to regulate the phenotype and biosynthetic activity of chondrocytes.

Published
2015

13. Regional comparisons of porcine menisci

Author

Zhang, Xiangkai, primary, Aoyama, Tomoki, additional, Ito, Akira, additional, Tajino, Junichi, additional, Nagai, Momoko, additional, Yamaguchi, Shoki, additional, Iijima, Hirotaka, additional, and Kuroki, Hiroshi, additional

Published
2014
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15. Exploring the role of mechanical forces on tendon development using in vivo model: A scoping review.

Author

Usami Y, Iijima H, and Kokubun T

Subjects
Animals, Biomechanical Phenomena physiology, Humans, Tendons physiology
Abstract

Tendons transmit the muscle contraction forces to bones and drive joint movement throughout life. While extensive research have indicated the essentiality of mechanical forces on tendon development, a comprehensive understanding of the fundamental role of mechanical forces still needs to be impaerted. This scoping review aimed to summarize the current knowledge about the role of mechanical forces during the tendon developmental phase. The electronic database search using PubMed, performed in May 2023, yielded 651 articles, of which 16 met the prespecified inclusion criteria. We summarized and divided the methods to reduce the mechanical force into three groups: loss of muscle, muscle dysfunction, and weight-bearing regulation. In contrast, there were few studies to analyze the increased mechanical force model. Most studies suggested that mechanical force has some roles in tendon development in the embryo to postnatal phase. However, we identified species variability and methodological heterogeneity to modulate mechanical force. To establish a comprehensive understanding, methodological commonality to modulate the mechanical force is needed in this field. Additionally, summarizing chronological changes in developmental processes across animal species helps to understand the essence of developmental tendon mechanobiology. We expect that the findings summarized in the current review serve as a groundwork for future study in the fields of tendon developmantal biology and mechanobiology., (© 2023 The Authors. Developmental Dynamics published by Wiley Periodicals LLC on behalf of American Association for Anatomy.)

Published
2024
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