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Your search keyword '"Iavarone, Federica"' showing total 20 results
Start Over Author "Iavarone, Federica" Publisher wiley
20 results on '"Iavarone, Federica"'

2. A top‐down proteomic approach reveals a salivary protein profile able to classify Parkinson's disease with respect to Alzheimer's disease patients and to healthy controls

Author

Contini, Cristina, primary, Fadda, Laura, additional, Lai, Greca, additional, Masala, Carla, additional, Olianas, Alessandra, additional, Castagnola, Massimo, additional, Messana, Irene, additional, Iavarone, Federica, additional, Bizzarro, Alessandra, additional, Masullo, Carlo, additional, Solla, Paolo, additional, Defazio, Giovanni, additional, Manconi, Barbara, additional, Diaz, Giacomo, additional, and Cabras, Tiziana, additional

Published
2023
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3. A top-down proteomic approach reveals a salivary protein profile able to classify Parkinson's disease with respect to Alzheimer's disease patients and to healthy controls

Author

Contini, C., Fadda, L., Lai, Giampaolo, Masala, C., Olianas, A., Castagnola, Massimo, Messana, Irene, Iavarone, Federica, Bizzarro, Alessandra, Masullo, Carlo, Solla, P., Defazio, G., Manconi, B., Diaz, G., Cabras, T., Lai G., Castagnola M. (ORCID:0000-0002-0959-7259), Messana I. (ORCID:0000-0002-1436-6105), Iavarone F. (ORCID:0000-0002-2074-5531), Bizzarro A., Masullo C. (ORCID:0000-0001-7798-3410), Contini, C., Fadda, L., Lai, Giampaolo, Masala, C., Olianas, A., Castagnola, Massimo, Messana, Irene, Iavarone, Federica, Bizzarro, Alessandra, Masullo, Carlo, Solla, P., Defazio, G., Manconi, B., Diaz, G., Cabras, T., Lai G., Castagnola M. (ORCID:0000-0002-0959-7259), Messana I. (ORCID:0000-0002-1436-6105), Iavarone F. (ORCID:0000-0002-2074-5531), Bizzarro A., and Masullo C. (ORCID:0000-0001-7798-3410)

Abstract

Parkinson's disease (PD) is a complex neurodegenerative disease with motor and non-motor symptoms. Diagnosis is complicated by lack of reliable biomarkers. To individuate peptides and/or proteins with diagnostic potential for early diagnosis, severity and discrimination from similar pathologies, the salivary proteome in 36 PD patients was investigated in comparison with 36 healthy controls (HC) and 35 Alzheimer's disease (AD) patients. A top-down platform based on HPLC-ESI-IT-MS allowed characterizing and quantifying intact peptides, small proteins and their PTMs (overall 51). The three groups showed significantly different protein profiles, PD showed the highest levels of cystatin SA and antileukoproteinase and the lowest of cystatin SN and some statherin proteoforms. HC exhibited the lowest abundance of thymosin & beta;4, short S100A9, cystatin A, and dimeric cystatin B. AD patients showed the highest abundance of & alpha;-defensins and short oxidized S100A9. Moreover, different proteoforms of the same protein, as S-cysteinylated and S-glutathionylated cystatin B, showed opposite trends in the two pathological groups. Statherin, cystatins SA and SN classified accurately PD from HC and AD subjects. & alpha;-defensins, histatin 1, oxidized S100A9, and P-B fragments were the best classifying factors between PD and AD patients. Interestingly statherin and thymosin & beta;4 correlated with defective olfactory functions in PD patients. All these outcomes highlighted implications of specific proteoforms involved in the innate-immune response and inflammation regulation at oral and systemic level, suggesting a possible panel of molecular and clinical markers suitable to recognize subjects affected by PD.

Published
2023

4. Trypsinogen and chymotrypsinogen: the mysterious hyper-reactivity of selected cysteines is still present after their divergent evolution

Author

Cattani, Giada, Bocedi, Alessio, Gambardella, Giorgia, Iavarone, Federica, Boroumand, Mozhgan, Castagnola, Massimo, Ricci, Giorgio, Iavarone, Federica (ORCID:0000-0002-2074-5531), Castagnola, Massimo (ORCID:0000-0002-0959-7259), Cattani, Giada, Bocedi, Alessio, Gambardella, Giorgia, Iavarone, Federica, Boroumand, Mozhgan, Castagnola, Massimo, Ricci, Giorgio, Iavarone, Federica (ORCID:0000-0002-2074-5531), and Castagnola, Massimo (ORCID:0000-0002-0959-7259)

Abstract

An enigmatic and never described hyper-reactivity of most of the cysteines resident in the reduced, molten globule-like intermediate of a few proteins has been recently discovered. In particular, all ten cysteines of chymotrypsinogen showed hundred times increased reactivity against hydrophobic reagents. A single cysteine (Cys1) was also found thousand times more reactive toward GSSG, making speculate that a single glutathionylation could represent the primordial event of its oxidative folding. In the present study, we compare these kinetic properties with those present in trypsinogen taken in its reduced, molten globule-like intermediate and identify the origin of these unusual properties. Despite the divergent evolution of these two proteins, the different amount of disulfides and the very different 3D localization of three disulfides, their hyper-reactivity toward hydrophobic thiol reagents and disulfides is very similar. Mass spectrometry identifies two cysteines in trypsinogen, Cys148 and Cys197, 800 times more reactive toward GSSG than an unperturbed protein cysteine. These results point toward a stringent and accurate preservation of these peculiar kinetic properties during a divergent evolution suggesting some important role, which at the present can only be hypothesized. Similar extraordinary hyper-reactivity has been found also in albumin, ribonuclease, and lysozyme confirming that it cannot be considered a kinetic singularity of a single protein. Interestingly, the very flexible and fluctuating structures like those typical of the molten globule status prove capable of enabling sophisticated actions typical of enzymes such as binding to GSSG with relevant specificity and high affinity (K-D = 0.4 mm) and accelerating the reaction of its cysteines by thousands of times.

Published
2021

5. Investigation by top‐down high‐performance liquid chromatography–mass spectrometry of glutathionylation and cysteinylation of salivary S100A9 and cystatin B in preterm newborns

Author

Boroumand, Mozghan, primary, Manconi, Barbara, additional, Serrao, Simone, additional, Iavarone, Federica, additional, Olianas, Alessandra, additional, Cabras, Tiziana, additional, Contini, Cristina, additional, Pieroni, Luisa, additional, Sanna, Maria Teresa, additional, Vento, Giovanni, additional, Tirone, Chiara, additional, Desiderio, Claudia, additional, Fiorita, Antonella, additional, Faa, Gavino, additional, Messana, Irene, additional, and Castagnola, Massimo, additional

Published
2021
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7. Enrichments of post‐translational modifications in proteomic studies

Author

Pieroni, Luisa, primary, Iavarone, Federica, additional, Olianas, Alessandra, additional, Greco, Viviana, additional, Desiderio, Claudia, additional, Martelli, Claudia, additional, Manconi, Barbara, additional, Sanna, Maria Teresa, additional, Messana, Irene, additional, Castagnola, Massimo, additional, and Cabras, Tiziana, additional

Published
2019
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9. N- and O-linked glycosylation site profiling of the human basic salivary proline-rich protein 3M

Author

Manconi, Barbara, primary, Cabras, Tiziana, additional, Sanna, Monica, additional, Piras, Valentina, additional, Liori, Barbara, additional, Pisano, Elisabetta, additional, Iavarone, Federica, additional, Vincenzoni, Federica, additional, Cordaro, Massimo, additional, Faa, Gavino, additional, Castagnola, Massimo, additional, and Messana, Irene, additional

Published
2016
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10. Lipoaspirate fluid proteome: A preliminary investigation by LC-MS top-down/bottom-up integrated platform of a high potential biofluid in regenerative medicine

Author

Inserra, Ilaria, primary, Martelli, Claudia, additional, Cipollina, Mara, additional, Cicione, Claudia, additional, Iavarone, Federica, additional, Di Taranto, Guiseppe, additional, Barba, Marta, additional, Castagnola, Massimo, additional, Desiderio, Claudia, additional, and Lattanzi, Wanda, additional

Published
2016
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11. Proteomic characterization of pediatric craniopharyngioma intracystic fluid by LC-MS top-down/bottom-up integrated approaches

Author

Martelli, Claudia, primary, Iavarone, Federica, additional, Vincenzoni, Federica, additional, Rossetti, Diana Valeria, additional, D'Angelo, Luca, additional, Tamburrini, Gianpiero, additional, Caldarelli, Massimo, additional, Di Rocco, Concezio, additional, Messana, Irene, additional, Castagnola, Massimo, additional, and Desiderio, Claudia, additional

Published
2014
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12. High-resolution high-performance liquid chromatography with electrospray ionization mass spectrometry and tandem mass spectrometry characterization of a new isoform of human salivary acidic proline-rich proteins named Roma-Boston Ser22 (Phos) → Phe variant

Author

Iavarone, Federica, primary, D'Alessandro, Alfredo, additional, Tian, Na, additional, Cabras, Tiziana, additional, Messana, Irene, additional, Helmerhorst, Eva J., additional, Oppenheim, Frank G., additional, and Castagnola, Massimo, additional

Published
2014
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14. Top‐down HPLC–ESI‐MS characterization of rat gliadoralin A, a new member of the family of rat submandibular gland glutamine‐rich proteins and potential substrate of transglutaminase

Author

Cabras, Tiziana, primary, Iavarone, Federica, additional, Pirolli, Davide, additional, Cristina De Rosa, Maria, additional, Vitali, Alberto, additional, Faa, Gavino, additional, Cordaro, Massimo, additional, Messana, Irene, additional, Ekström, Jörgen, additional, and Castagnola, Massimo, additional

Published
2013
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16. Enzymatic processing by MMP‐2 and MMP‐9 of wild‐type and mutated mouse β‐dystroglycan

Author

Sbardella, Diego, primary, Inzitari, Rosanna, additional, Iavarone, Federica, additional, Gioia, Magda, additional, Marini, Stefano, additional, Sciandra, Francesca, additional, Castagnola, Massimo, additional, Van den Steen, Philippe E., additional, Opdenakker, Ghislain, additional, Giardina, Bruno, additional, Brancaccio, Andrea, additional, Coletta, Massimo, additional, and Bozzi, Manuela, additional

Published
2012
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