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22. ChemInform Abstract: Studies on Water-Soluble Artificial Receptors Containing Chiral Side Chains Derived from Carbohydrates. Part 1. Synthesis of Optically Active Cyclophane TCP44 and Its Complexation Selectivity for Aromatic Guests in Acidic Aqueous Solu

Author

TAKAHASHI, I., primary, HIRANO, Y., additional, ARAKAWA, H., additional, KITAJIMA, H., additional, HATANAKA, M., additional, ISA, K., additional, ODASHIMA, K., additional, and KOGA, K., additional

Published
2010
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38. Induction of c-fos and c-myc mRNA expression by immunoglobulin G from patients with Graves’ disease in thyrotrophin-dependent rat thyroid cell line (FRTL5)

Author

Hatabu, H., primary, Kasagl, K., additional, Lida, Y., additional, Nosaka, T., additional, Misaki, T., additional, Hidaka, A., additional, Tokuda, Y., additional, Endo, K., additional, Mori, T., additional, Lee, K., additional, Tsubol, K., additional, Hatanaka, M., additional, and Konishl, J., additional

Published
1991
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43. Identification of HTLV-I gag protease and its sequential processing of the gag gene product

Author

Seok Hyun Nam and Hatanaka M

Subjects
viruses, medicine.medical_treatment, Blotting, Western, Molecular Sequence Data, Biology, Transfection, Biochemistry, law.invention, Viral Proteins, Retrovirus, HIV Protease, law, Endopeptidases, medicine, Retroviral aspartyl protease, Amino Acid Sequence, Molecular Biology, Expression vector, Protease, Base Sequence, Hydrolysis, Nucleic acid sequence, Cell Biology, Group-specific antigen, Provirus, biology.organism_classification, Molecular biology, DNA, Viral, Recombinant DNA
Abstract

The full-length provirus of human T-cell leukemia virus type I (HTLV-I) was isolated from MT-2, a lymphoid cell line producing HTLV-I. In transfected cells, structural proteins of HTLV-I, the gag and env products, were formed and processed in the same manner as observed in MT-2 cells. The nucleotide sequence was determined for a region between the gag and pol genes of the proviral DNA clone containing an open-reading frame. The deduced amino acid sequences show that this open-reading frame encodes a putative HTLV-I protease. The protease gene (pro) of HTLV-I was investigated using a vaccinia virus expression vector. Processing of 53k gag precursor polyprotein into mature p19, p24, and p15 gag structural proteins was detectable with a recombinant plasmid harboring the entire gag- and protease-coding sequence. We demonstrated that the protease processed the gag precursor polyprotein in a trans-action. A change in the sequence Asp(64)-Thr-Gly, the catalytic core sequence among aspartyl proteases, to Gly-Thr-Gly was shown to abolish correct processing, suggesting that HTLV-I protease may belong to the aspartyl protease group. The 76k gag-pro precursor polyprotein was identified, implying that a cis-acting function of HTLV-I protease may be necessary to trigger the initial cleavage event for its own release from a precursor protein, followed by the release of p53 gag precursor protein. The p53 gag precursor protein is then processed by the trans-action of the released protease to form p19, p24, and p15.

Published
1989
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44. Low-titer antibodies reactive with HTLV-I gag P19 in patients with chronic myeloneuropathy

Author

Taketo Kawanishi, Masashi Fujita, Ichiro Akiguchi, Hatanaka M, and Masakuni Kameyama

Subjects
Adult, Male, Pathology, medicine.medical_specialty, Multiple Sclerosis, Adolescent, Immunoglobulin gamma-Chains, Polyradiculoneuropathy, Polymyositis, Virus, Serology, Myelopathy, medicine, Humans, Aged, biology, business.industry, Multiple sclerosis, Middle Aged, medicine.disease, Myasthenia gravis, HTLV-I Antibodies, Neurology, Immunology, biology.protein, Female, Neurology (clinical), Antibody, business, Demyelinating Diseases
Abstract

To elucidate the possible association of human T-lymphotropic virus type I (HTLV-I) and chronic neurological diseases, 156 serum samples from patients with various neurological diseases, including multiple sclerosis, chronic progressive myelopathy, chronic inflammatory polyradiculoneuropathy, myasthenia gravis, polymyositis, motor neuron disease, and tension headache, and healthy control subjects were examined for IgG antibodies to HTLV-I by three independent techniques--gelatin particle agglutination test, enzyme-linked immunosorbent assay, and Western blot assay. Specificity of antibodies was assessed by homologous competitive inhibition on Western blot assay. Six patients (3 with chronic progressive myelopathy, 1 with chronic inflammatory polyradiculoneuropathy, 1 with motor neuron disease, and 1 with tension headache) had high-titer HTLV-I antibodies. Twelve patients (5 with multiple sclerosis, 1 with chronic progressive myelopathy, 2 with chronic inflammatory polyradiculoneuropathy, 2 with myasthenia gravis, and 2 with motor neuron disease) had low-titer HTLV-I antibodies that reacted with a single gag protein, p19 or p24, on Western blot assay. In 4 (2 with multiple sclerosis, 1 with chronic progressive myelopathy, and 1 with chronic inflammatory polyradiculoneuropathy) of these 12, the antibodies that were all directed to p19 were determined to be specific by homologous competitive inhibition. In the remaining 8 patients (3 with multiple sclerosis, 1 with chronic inflammatory polyradiculoneuropathy, 2 with myasthenia gravis, and 2 with motor neuron disease), restricted reactions against p19 or p24 were considered to be nonspecific because they were not inhibited by homologous competitive inhibition. The results suggest that in some patients chronic myeloneuropathy diagnosed as chronic progressive multiple sclerosis, chronic progressive myelopathy, and chronic inflammatory polyradiculoneuropathy may be associated with HTLV-I or related retroviruses.

Published
1989
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