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14 results on '"Harder, M"'

1. Timing and direction of faunal exchange between the Nearctic and the Palaearctic in Odonata

Author

Pàmies‐Harder, M. (Maria), Múrria, Cesc, Abbott, John C., Abbott, Kendra, Kalkman, V.J. (Vincent), Pàmies‐Harder, M. (Maria), Múrria, Cesc, Abbott, John C., Abbott, Kendra, and Kalkman, V.J. (Vincent)

Abstract

Aim Species have different distribution patterns across the globe and among biogeographical regions. The Nearctic and Palaearctic regions share lineages because of their parallel biogeographic histories and ecological conditions. As the number of phylogenetic studies increases, there are more insights into past exchange events between these two regions and their effects on the current distribution of diversity. However, several groups have not been tested and an overall generalization is still missing. Here, we analyse the biogeographic history across multiple genera of odonates to elucidate a general process of species exchange, vicariance and species divergence between these two regions. Location The Holarctic, including the entire Nearctic and the East and West Palaearctic. Taxon 14 genera of Odonata (Insecta). Methods We reconstructed a time-calibrated phylogenetic tree for each genus to determine species relationships and divergence time using 3614 COI sequences of 259 species. Biogeographic ancestral range estimation was inferred for each phylogeny using BioGeoBEARS. Preferred habitat (lotic versus lentic) was established for each species. Results Exchange events were not restricted in time, direction or either lentic habitat or lotic habitat. Most genera crossed between both regions only once, and it was mainly across the Beringia, while three diverse anisopteran genera revealed multiple exchanges. Recent exchanges during the Pleistocene were associated with cold-dwelling and lentic species. Main Conclusions Our finding reveals the absence of a generalizable pattern of species exchange and divergence between the Nearctic and Palaearctic regions; instead, we found lineage-specific biogeographic patterns. This finding highlights the complexity of drivers and functional traits that shaped current diversity patterns. Moreover, it emphasizes that general conclusions cannot be formulated based on one single clade.

Published
2024
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11. Stability evaluation of morphine, hydromorphone, metamizole and esketamine containing analgesic mixtures applied for patient-controlled analgesia in hospice and palliative care.

Author

Harder M, Fiegl-Lechner A, Oberacher H, Horvath UEI, Schlager A, Jeske M, Kerndler S, Schüllner F, Bonn GK, and Rainer M

Subjects
Analgesia, Patient-Controlled, Analgesics, Opioid, Dipyrone, Humans, Ketamine, Morphine, Palliative Care methods, Hospices, Hydromorphone chemistry
Abstract

In this study, different injection solutions containing opioid and nonopioid compounds used for patient-controlled analgesia in hospice and palliative care were evaluated in terms of analyte stability. Investigated injection solutions contained different combinations of morphine, hydromorphone, metamizole and esketamine. For the practical implementation, samples from infusion pumps were daily drawn over a period of 7 days at 22 and 37°C. Quantitative measurements were performed on a high-performance liquid chromatography system with ultraviolet detection applying a validated analytical method. All compounds apart from morphine showed no evident changes in concentration. However, a significant loss of morphine was observed for injection mixtures containing both morphine and metamizole at 37°C. After 7 days, only 72% of the initially measured morphine concentration was measured in the binary and 77% in the ternary mixture. Furthermore, an additional compound was detected that could represent the morphine-metamizole-adduct, "metamorphine". Based on these results, a significantly reduced morphine concentration must be expected after only 3 days if an injection solution mixture containing both morphine and metamizole is administered to a patient at 37°C. Since the analgesic effects of morphine-metamizole adducts have not yet been thoroughly investigated, further clinical studies are necessary before accurate conclusions can be drawn in this regard., (© 2022 The Authors. Biomedical Chromatography published by John Wiley & Sons Ltd.)

Published
2022
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12. Correlation between Z score, fetal fraction, and sequencing reads in non-invasive prenatal testing.

Author

Balslev-Harder M, Richter SR, Kjaergaard S, and Johansen P

Subjects
Blood Chemical Analysis methods, Blood Chemical Analysis standards, Cell-Free Nucleic Acids blood, Down Syndrome blood, Down Syndrome diagnosis, Female, Gestational Age, Humans, Male, Maternal Serum Screening Tests standards, Pregnancy, Prenatal Diagnosis standards, Prenatal Diagnosis statistics & numerical data, Research Design, Trisomy diagnosis, Trisomy genetics, Cell-Free Nucleic Acids analysis, Data Interpretation, Statistical, Fetus metabolism, High-Throughput Nucleotide Sequencing methods, High-Throughput Nucleotide Sequencing standards, Prenatal Diagnosis methods
Published
2017
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14. Open source non-invasive prenatal testing platform and its performance in a public health laboratory.

Author

Johansen P, Richter SR, Balslev-Harder M, Miltoft CB, Tabor A, Duno M, and Kjaergaard S

Subjects
Adult, Chromosomes, Human, Pair 13, Chromosomes, Human, Pair 18, DNA genetics, Denmark, Female, High-Throughput Nucleotide Sequencing instrumentation, Humans, Male, Pregnancy, Prenatal Diagnosis, Public Health, Semiconductors, Sequence Analysis, DNA instrumentation, Sex Determination Analysis, Trisomy 13 Syndrome, Trisomy 18 Syndrome, Chromosome Disorders diagnosis, DNA blood, Down Syndrome diagnosis, High-Throughput Nucleotide Sequencing methods, Maternal Serum Screening Tests methods, Sequence Analysis, DNA methods, Trisomy diagnosis
Abstract

Objective: The objective of this study was to introduce non-invasive prenatal testing (NIPT) for fetal autosomal trisomies and gender in a Danish public health setting, using semi-conductor sequencing and published open source scripts for analysis., Methods: Plasma-derived DNA from a total of 375 pregnant women (divided into three datasets) was whole-genome sequenced on the Ion Proton™ platform and analyzed using a pipeline based on WISECONDOR for fetal autosomal aneuploidy detection and SeqFF for fetal DNA fraction estimation. We furthermore validated a fetal sex determination analysis., Results: The pipeline correctly detected 27/27 trisomy 21, 4/4 trisomy 18, and 3/3 trisomy 13 fetuses. Neither false negatives nor false positives (chromosomes 13, 18, and 21) were observed in our validation dataset. Fetal sex was identified correctly in all but one triploid fetus (172/173). SeqFF showed a strong correlation (R(2)  = 0.72) to Y-chromosomal content of the male fetus samples., Discussion: We have implemented NIPT into Danish health care using published open source scripts for autosomal aneuploidy detection and fetal DNA fraction estimation showing excellent false negative and false positive rates. SeqFF provides a good estimation of fetal DNA fraction. This coupled with an analysis of fetal sex that provides a complete NIPT workflow, which may easily be adapted for implementation in other public health laboratories. © 2016 John Wiley & Sons, Ltd., (© 2016 John Wiley & Sons, Ltd.)

Published
2016
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