12 results on '"H, Yamakawa"'
Search Results
2. Trypsin-like protease of mites: purification and characterization of trypsin-like protease from mite faecal extract Dermatophagoides farinae. Relationship between trypsin-like protease and Der f III
- Author
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M. Iwaki, S. Ikeda, A Miyahara, R. Homma, Toru Ando, Michiko Haida, H Kimura, G Ito, H. Yamakawa, Matsunobu Suko, T. Yanagihara, Yasushi Okumura, Y. Ino, and Hirokazu Okudaira
- Subjects
Male ,medicine.medical_treatment ,Guanidines ,Substrate Specificity ,Feces ,chemistry.chemical_compound ,Immunology and Allergy ,Aprotinin ,Amino Acids ,Peptide sequence ,chemistry.chemical_classification ,Mites ,biology ,Serine Endopeptidases ,Temperature ,Hydrogen-Ion Concentration ,Middle Aged ,Chromatography, Ion Exchange ,Amino acid ,Biochemistry ,Chromatography, Gel ,Female ,Trypsin Inhibitors ,medicine.drug ,Adult ,Serine Proteinase Inhibitors ,Adolescent ,Iodoacetic acid ,Trypsin inhibitor ,Molecular Sequence Data ,Immunology ,Enzyme-Linked Immunosorbent Assay ,Arthropod Proteins ,medicine ,Animals ,Humans ,Amino Acid Sequence ,Aged ,Serine protease ,Protease ,Sequence Homology, Amino Acid ,Allergens ,Antigens, Plant ,Benzamidines ,Molecular Weight ,Enzyme ,chemistry ,biology.protein ,Digestive System ,Sequence Alignment - Abstract
Summary A serine protease from mite faecal extract, Dermatophagoides farinae, was purified using DEAE-Sephacel anion exchange chromatography and Supcrdex 75 pg gel chromato-graphy. The molecular weight of this protease was 34 kD on SDS-PAGE under reducing conditions. The optimal pH and temperature of the protease were 8-0 and 47 C, respectively. In addition, this protease cleaved arginyl or lysyl residue containing substrates selectively and was only inhibited by aprotinin, PUT-175, tind soy bean trypsin inhibitor and not by chymostatin, E-64 and iodoacetic acid. These results show that our purified serine protease belongs to the trypsin-type. Purified trypsin-like protease was shown to be allergenic by enzyme-linked immunosorbent assay. Antigeni-city of trypsin-like protease was completely different from those of Der f I and Der f II. Both, 20 N-terminal amino acid sequence and amino acid compositions of the purified protease were very similar to those of Der f III. Good similarities were found between trypsin-like protease and Der f III concerning physicochemical properties such as molecular weight on SDS-PAGE and ammonium sulphate solubility. Summarizing the above data, it can be concluded that a trypsin-like protease from mite faecal extract is actually the Der f III allergen and that it may be involved in the digestive process of the mite as it was found not in mite body but in mite faeces.
- Published
- 1993
3. 29.2: High-Rate Deposition of MgO Films for ACPDPS by Activated Reactive Evaporation Using Hollow-Cathode Discharge (HCD-ARE)
- Author
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M. Hakomori, M. Matsuura, K. Matsuzaki, H. Yamakawa, and T. Kurauchi
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High rate ,Materials science ,Chemical engineering ,law ,Analytical chemistry ,Deposition (phase transition) ,Thermal damage ,Substrate (electronics) ,Evaporation (deposition) ,Cathode ,law.invention - Abstract
We have developed a new MgO film synthesizing method employing HCD-ARE which enables high film deposition rates up to 73nmls. This method was found to prevent thermal damage to the glass substrate during deposition. The discharge voltage characteristics of AC-PDPS protected by MgO film prepared by HCD-ARE were determined to be sufficient for practical application.
- Published
- 1998
4. Interleukin-1-induced glenohumeral synovitis and shoulder pain in rotator cuff diseases.
- Author
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Gotoh M, Hamada K, Yamakawa H, Yanagisawa K, Nakamura M, Yamazaki H, Inoue A, and Fukuda H
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- Adult, Aged, Female, Gene Expression, Humans, Male, Middle Aged, Pain immunology, Pain pathology, Pain physiopathology, RNA, Messenger analysis, Rotator Cuff immunology, Rotator Cuff pathology, Shoulder Joint immunology, Shoulder Joint pathology, Shoulder Joint physiopathology, Synovitis immunology, Synovitis pathology, Interleukin-1 genetics, Rotator Cuff physiopathology, Synovitis physiopathology
- Abstract
Synovitis of the subacromial bursa has been identified as a main source of shoulder pain in rotator cuff diseases. Little interest, however, has been paid into the synovitis of glenohumeral joint. The mRNA expression levels of interleukin-1beta (IL-1beta) and interleukin-1 receptor antagonists produced in the synovitis reflect the magnitude of inflammation. The present study was undertaken to determine the relationship between mRNA expression levels of IL-1beta and its receptor antagonists (secreted interleukin-1 receptor antagonist (IL-1ra) and intracellular IL-1ra) in the synovium of the glenohumeral joint and shoulder pain in rotator cuff diseases, analyzing the synovial specimens by reverse transcriptase polymerase chain reaction. Thirty-five patients with rotator cuff diseases were candidates. Based on the presence of cuff perforation, they were divided into two categories: 16 with non-perforating tears and 19 with perforating tears. The degree of shoulder pain was evaluated by use of a visual analogue scale. The pain degree of non-perforating tears was significantly greater than that of perforating tears (P < 0.01). In contrast, the expression levels of the cytokine-mRNAs were constitutively greater in perforating tears than in non-perforating tears (P < 0.01, respectively). The expression levels of the cytokine-mRNAs were inversely correlated with the degree of pain (IL-1beta: r = 0.930; secreted IL-1ra: r = 0.861; intracellular IL-1ra: r = 0.932, P < 0.001 respectively). These results suggest that the expression levels of the cytokine-mRNAs in the synovium of the glenohumeral joint contribute less to the generation of shoulder pain in rotator cuff diseases.
- Published
- 2002
- Full Text
- View/download PDF
5. Healing of full-thickness tears of avian supracoracoid tendons: in situ hybridization of alpha1(I) and alpha1(III) procollagen mRNA.
- Author
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Kobayashi K, Hamada K, Gotoh M, Handa A, Yamakawa H, and Fukuda H
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- Animals, Chickens, Coloring Agents, Disease Models, Animal, Eosine Yellowish-(YS), Gene Expression physiology, Hematoxylin, In Situ Hybridization, Lacerations physiopathology, Lacerations surgery, RNA, Messenger analysis, Rotator Cuff surgery, Sutures, Tendon Injuries surgery, Procollagen genetics, Rotator Cuff physiology, Rotator Cuff Injuries, Tendon Injuries physiopathology, Wound Healing physiology
- Abstract
Although clinically significant, much remains unknown about the healing of the torn rotator cuff tendon, because of unavailability of appropriate animal model. The human supraspinatus tendon faces the subacromial bursa superiorly, and the joint capsule inferiorly, while the digital flexor tendon is surrounded by the synovium. We hypothesized that the supraspinatus tendon heals by the process which is different from that observed with digital flexor tendons, in which epitenon cells migrate into the torn portion circumferentially. The avian supracoracoid tendon was adopted for this experiment because of its similarity to the human supraspinatus tendon. We developed a full-thickness tendon laceration followed by primary suture. The objective of this study was to detect localization of the responsible cells for repair of the tendon. We examined the process using histology and in situ hybridization. Starting at week 1 the peritendon cells of the bursal side proliferated and migrated into the laceration site. At week 6, the tendon stumps were continuous with new connective tissue. High-level expression of procollagen mRNA in the proliferating peritendon cells on the bursal side demonstrates to contribute to the reparative process, which progressed to the joint side. This mode of repair is different from that of the digital flexor tendon.
- Published
- 2001
- Full Text
- View/download PDF
6. Increased interleukin-1beta production in the synovium of glenohumeral joints with anterior instability.
- Author
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Gotoh M, Hamada K, Yamakawa H, Nakamura M, Yamazaki H, Inoue A, and Fukuda H
- Subjects
- Acromion metabolism, Acromion pathology, Adult, Bursa, Synovial metabolism, Bursa, Synovial pathology, Female, Humans, Immunohistochemistry, Interleukin-1 genetics, Interleukin-1 metabolism, Joint Instability pathology, Male, RNA, Messenger metabolism, Reverse Transcriptase Polymerase Chain Reaction methods, Shoulder Joint pathology, Interleukin-1 biosynthesis, Joint Instability metabolism, Shoulder Joint metabolism, Synovial Membrane metabolism
- Abstract
Macroscopic synovitis of the glenohumeral joint is frequently seen during arthroscopy in patients with anterior instability. Interleukin-1beta is known to be expressed in inflamed tissue, to correlate with the magnitude of inflammation, and to affect articular cartilage in the joint. We hypothesized that chronic synovitis may occur in the glenohumeral joint in patients with anterior instability. The purpose of this study was to examine the expression of interleukin-1beta in the synovium of the glenohumeral joint with anterior instability and to discuss its clinicopathologic significance. Specimens of synovial tissue around the greater tuberosity in the subacromial synovium (as controls) and around the rotator interval in the glenohumeral synovium were obtained from 10 patients who had anterior instability without signs of subacromial impingement. Semiquantitative reverse transcriptase-polymerase chain reaction was used to compare the levels of interleukin-1beta mRNA expression in the glenohumeral joint with those in the subacromial bursa. We also employed immunohistochemistry and in situ reverse transcriptase-polymerase chain reaction to detect the cells producing interleukin-1beta protein and mRNA. The levels of interleukin-1beta mRNA expression were significantly higher in the glenohumeral joint than in the subacromial bursa (p < 0.01). Histology showed nonspecific inflammation in all 10 samples of glenohumeral synovium, whereas no inflammation was seen in seven of 10 samples of subacromial synovium. Immunohistochemistry identified interleukin-1beta protein in the vessels and inflammatory and synovial cells (from lining to sublining layers) in synovium of the glenohumeral joint, whereas immunoreactivity was negative in seven subacromial bursa. The remaining three synovial specimens of subacromial bursa, however, showed positive immunoreactivity that was unremarkable and confined around the vessels. In situ reverse transcriptase-polymerase chain reaction was exclusively performed in the synovial specimens of the glenohumeral joint, which exhibited a positive reaction (in the same kinds of cells as seen with immunohistochemistry) in the lining and sublining layers and to a lesser extent in the stroma. Thus, our data confirmed the increased production of interleukin-1beta in the synovium of the glenohumeral joint in patients with anterior instability, suggesting the presence of chronic inflammation at the site. We conclude that this chronic synovitis may be partly associated with the development of dislocation arthropathy in the long term.
- Published
- 1999
- Full Text
- View/download PDF
7. Increased substance P in subacromial bursa and shoulder pain in rotator cuff diseases.
- Author
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Gotoh M, Hamada K, Yamakawa H, Inoue A, and Fukuda H
- Subjects
- Adult, Aged, Antibodies, Bursa, Synovial chemistry, Bursa, Synovial physiopathology, Bursitis physiopathology, Humans, Middle Aged, Pain metabolism, Pain Measurement, Rotator Cuff chemistry, Rotator Cuff physiopathology, Substance P analysis, Substance P immunology, Bursa, Synovial metabolism, Bursitis metabolism, Rotator Cuff Injuries, Shoulder Joint physiopathology, Substance P metabolism
- Abstract
The subacromial bursa is recognized as a site associated with the shoulder pain caused by rotator cuff disease in the middle-aged and elderly. Substance P is contained in primary afferent nerves, and its quantity increases during chronic pain. The amount of substance P in the subacromial bursa of patients with rotator cuff disease was examined. Radioimmunoassay and immunohistochemistry were employed to quantify and localize substance P. The preoperative pain level was measured with a visual analogue scale with 0 as no pain, 5 as moderate, and 10 as severe. Thirty-seven patients that had undergone operation were divided into two groups: one composed of 19 patients with subacromial bursitis and a partial-thickness tear of the rotator cuff (nonperforated cuff) and the other composed of 18 patients with a full-thickness tear (perforated cuff). Subacromial bursae obtained from seven fresh cadavers with no shoulder pain before death were used as controls. The visual analogue scale showed significantly greater pain in the group with the nonperforated rotator cuff than in the group with the perforated cuff. Consistent with these results, the amount of substance P in the subacromial bursa was significantly greater in the former group than in the latter. Nerve fibers immunoreactive to substance P were localized around the vessels, with a larger number of fibers in the group with the nonperforated rotator cuff. Therefore, an increased amount of substance P in the subacromial bursa appears to correlate with the pain caused by rotator cuff disease.
- Published
- 1998
- Full Text
- View/download PDF
8. Intrinsic healing capacity and tearing process of torn supraspinatus tendons: in situ hybridization study of alpha 1 (I) procollagen mRNA.
- Author
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Hamada K, Tomonaga A, Gotoh M, Yamakawa H, and Fukuda H
- Subjects
- Adult, Aged, Antisense Elements (Genetics), Blotting, Northern, Humans, In Situ Hybridization, Middle Aged, RNA, Messenger analysis, Ribonucleases, Tendon Injuries pathology, Tendons chemistry, Tendons pathology, Tendons physiology, Procollagen genetics, Tendon Injuries physiopathology, Wound Healing physiology
- Abstract
To determine the healing potential and healing process of torn supraspinatus tendons, in situ hybridization was used to localize cells containing alpha 1 type-I procollagen mRNA. Biopsy specimens of torn supraspinatus tendons from 19 patients with complete-thickness tears and 13 patients with incomplete-thickness tears were obtained during surgery. Four macroscopically normal supraspinatus tendons were obtained to serve as normal controls. Specimens were fixed in 10% buffered formalin and embedded in paraffin. A 22-mer oligonucleotide probe was labeled with digoxigenin and used as an in situ marker. The labeled cells were mainly composed of tenocytes and undifferentiated mesenchymal cells. In complete-thickness-tears, the labeled cells at the proximal tendon-stumps in the specimens that were obtained less than 4 months after trauma were significantly more abundant than in the specimens obtained 4 months or more after trauma. However, the number of labeled cells was maintained at the torn portion even in long-standing incomplete-thickness tears. The labeled cells at the margins of concomitant intratendinous extensions of the tears were detected even in the long-standing tears. The intratendinous extensions exhibited more labeled cells than the bursal-side or joint-side layers of the tendon substance in the incomplete-thickness tears (p < 0.05). The torn supraspinatus tendon may possess an intrinsic healing capability in the intermediate and late phases of tendon healing. Incomplete-thickness tears and concomitant intratendinous extensions can continue to rupture after the initial injury.
- Published
- 1997
- Full Text
- View/download PDF
9. Significance of granulation tissue in torn supraspinatus insertions: an immunohistochemical study with antibodies against interleukin-1 beta, cathepsin D, and matrix metalloprotease-1.
- Author
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Gotoh M, Hamada K, Yamakawa H, Tomonaga A, Inoue A, and Fukuda H
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- Adult, Aged, Antibody Specificity, Cathepsin D immunology, Collagenases immunology, Granulation Tissue physiology, Humans, Immunohistochemistry, Interleukin-1 immunology, Male, Matrix Metalloproteinase 1, Middle Aged, Rotator Cuff chemistry, Rotator Cuff physiopathology, Rotator Cuff Injuries, Tendon Injuries physiopathology, Cathepsin D analysis, Collagenases analysis, Granulation Tissue chemistry, Interleukin-1 analysis, Tendon Injuries metabolism
- Abstract
The pathophysiology of rotator cuff tears can be elucidated by examining the tendinous insertion of the supraspinatus muscle. As seen by light microscopy, the granulation tissue around the insertion of a torn supraspinatus tendon appears to induce osteochondral destruction by means of multinucleated giant cells and chemical mediators. The purpose of this study was to examine the contribution of certain chemical mediators to osteochondral destruction using immunohistochemical analysis of interleukin-beta, cathepsin D, and matrix metalloprotease-1. Sixteen supraspinatus insertions with portions of the greater tuberosity, including eight complete-thickness tears and eight incomplete-thickness tears, were obtained during surgery. Six fresh cadaveric supraspinatus tendons without grossly evident tears served as normal controls. Strong immunoreactivity was found in all 16 torn supraspinatus insertions but not in the six insertions of apparently intact tendons. Macrophages and multinucleated giant cells, which showed immunoreactivity for all three chemical mediators, were often found at the interface between the osteochondral margin of the enthesis and the granulation tissue, suggesting that they may be involved in osteochondral destruction. We therefore concluded that, in addition to repetitive subacromial impingement, this granulation tissue may contribute to the development of rotator cuff tears by weakening the insertion.
- Published
- 1997
- Full Text
- View/download PDF
10. DNA-topoisomer analysis on the basis of the helical wormlike chain.
- Author
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Shimada J and Yamakawa H
- Subjects
- DNA, Circular, Isomerism, Mathematics, DNA, Nucleic Acid Conformation
- Published
- 1984
- Full Text
- View/download PDF
11. Sedimentation coefficients of DNA topoisomers: the helical wormlike chain.
- Author
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Shimada J and Yamakawa H
- Subjects
- Models, Theoretical, DNA, Nucleic Acid Conformation
- Published
- 1988
- Full Text
- View/download PDF
12. Moments for DNA topoisomers: the helical wormlike chain.
- Author
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Shimada J and Yamakawa H
- Subjects
- Models, Theoretical, Thermodynamics, DNA, Nucleic Acid Conformation
- Published
- 1988
- Full Text
- View/download PDF
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