1. A SERS quenching method for the sensitive determination of insulin
- Author
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Godwin A. Ayoko, Emad L. Izake, Mahnaz D. Gholami, and Prashant Sonar
- Subjects
Protein Conformation ,medicine.medical_treatment ,Metal Nanoparticles ,Pharmaceutical Science ,Spectrum Analysis, Raman ,Photochemistry ,Analytical Chemistry ,symbols.namesake ,chemistry.chemical_compound ,medicine ,Humans ,Insulin ,Environmental Chemistry ,In patient ,Benzothiazoles ,Disulfides ,Spectroscopy ,Detection limit ,Quenching ,Chemistry ,Reproducibility of Results ,Benzothiazole ,Colloidal gold ,symbols ,Gold ,Raman spectroscopy ,Oxidation-Reduction ,Disulphide bonds - Abstract
In this work, we utilise the disulphide bond structure of insulin and a new benzothiazole Raman probe for the detection of human insulin using surface-enhanced Raman spectroscopy (SERS). The disulphide bond structure of the insulin was reduced to generate free sulfhydryl terminal groups. When reacted with benzothiazole-functionalised gold nanoparticles, the reduced protein desorbs the Raman probe and causes its Raman signal intensity to quench. Using this approach, insulin was quantified in the concentration range of 1 × 10-14 -1 × 10-8 M by SERS quenching. The limit of quantification of insulin by the SERS quenching method was found to be 1 × 10-14 M (0.01 pM or 58 pg/L), which satisfies the requirements for monitoring its blood concentration in patients. Because many proteins and peptides have disulphide bonds in their molecular structures, the new SERS quenching method has a strong potential for the rapid determination of ultralow concentrations of proteins in formulations and biological fluids.
- Published
- 2020