1. Effect of folic acid-linked chitosan-coated PLGA-based curcumin nanoparticles on the redox system of glioblastoma cancer cells.
- Author
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Ghoreyshi N, Ghahremanloo A, Javid H, Homayouni Tabrizi M, and Hashemy SI
- Subjects
- Polylactic Acid-Polyglycolic Acid Copolymer metabolism, Polylactic Acid-Polyglycolic Acid Copolymer therapeutic use, Catalase, Glutaredoxins metabolism, Glutaredoxins therapeutic use, Antioxidants pharmacology, Reactive Oxygen Species metabolism, Reactive Oxygen Species therapeutic use, Folic Acid therapeutic use, Oxidation-Reduction, Superoxide Dismutase metabolism, Superoxide Dismutase therapeutic use, Thioredoxins metabolism, Thioredoxins therapeutic use, Curcumin pharmacology, Curcumin therapeutic use, Glioblastoma drug therapy, Chitosan metabolism, Chitosan therapeutic use, Nanoparticles
- Abstract
Objectives: Oxidative stress is one of the carcinogenic mechanisms underlying the development of glioblastoma multiforme (GBM), a highly aggressive brain tumor type associated with poor prognosis. Curcumin is known to be an efficient antioxidant, anti-inflammatory, and anticancer compound. However, its poor solubility in water, inappropriate pharmacokinetics, and low bioavailability limit its use as an antitumor drug. We prepared PLGA-based curcumin nanoparticles changed with folic acid and chitosan (curcumin-PLGA-CS-FA) and evaluated its effects on GBM tumor cells' redox status., Methods: The nanoprecipitation method was used to synthesize CU nanoparticles (CU-NPs). The size, morphology, and stability were characterized by DLS, SEM, and zeta potential analysis, respectively. The CU-NPs' toxic properties were studied by MTT assay and measuring the intracellular reactive oxygen species (ROS) and malondialdehyde (MDA) concentrations. The study was completed by measuring the gene expression levels and activity of superoxide dismutase, catalase, glutaredoxin, and thioredoxin antioxidant enzymes., Results: The size, polydispersity index, and zeta potential of CU-NPs were 77.27 nm, 0.29, and -22.45 mV, respectively. The encapsulation efficiency was approximately 98%. Intracellular ROS and MDA levels decreased after CU-NP treatment. Meanwhile, the CU-NPs increased gene expression and activity of superoxide dismutase, catalase, glutaredoxin, and thioredoxin antioxidant enzymes., Conclusion: CU-NPs might be effective in the prevention and treatment of glioblastoma cancer by modulating the antioxidant-oxidant balance., (© 2023 John Wiley & Sons Ltd.)
- Published
- 2023
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