48 results on '"Fang, Cui"'
Search Results
2. LncRNA LUCAT1 as a Plasma Biomarker for Assessing Disease Activity in Adult Patients with Crohn’s Disease
- Author
-
Xiao-yi Kuai, Shun-ying Yu, Xiu-fang Cui, Xiao-jing Zhao, Xia-qiong Mao, Yang Yu, Tong Hu, Hong-jie Zhang, and Chun-li Zhou
- Subjects
Diseases of the digestive system. Gastroenterology ,RC799-869 - Abstract
Aim. To explore the expression of long noncoding RNA (LncRNA) LUCAT1 in adult patients with Crohn’s disease (CD) and evaluate the relationship between LncRNA LUCAT1 and the disease activity in Chinese patients with CD. Methods. Patients with CD and healthy participants (≥18 years old) were enrolled in this study between January 2018 and December 2019. The expression of LncRNA LUCAT1 in plasma samples was evaluated by quantitative reverse transcription-polymerase chain reaction. Basic characteristics of patients with CD were collected, including gender, age, clinical stage, disease behavior, disease location, C-reactive protein (CRP), platelet (PLT), erythrocyte sedimentation rate (ESR), fecal calprotectin (FC), Crohn’s disease activity index (CDAI) score, and simplified Crohn’s disease endoscopic score (SES-CD). Results. In total, 168 patients with CD and 65 healthy participants (≥18 years old) were enrolled in this study. Among them, ninety patients with clinically active CD, seventy-eight patients with CD in clinical remission, forty-eight patients with endoscopically active CD, thirty patients with endoscopically inactive CD, and sixty-five healthy participants. LncRNA LUCAT1 was increased in plasma of patients with CD compared with the control group. The plasma LncRNA LUCAT1 level of patients with CD both in the clinical and endoscopic active phase was higher than that of both the clinical and endoscopic remission phase. The plasma level of LncRNA LUCAT1 in patients with CD was positively correlated with ESR, CRP, FC, CDAI, and SES-CD. There was no significant correlation between the level of LUCAT1 and platelets. The plasma LncRNA LUCAT1 level in patients with CD had significant differences between severe active patients and mild/moderate active patients. Conclusion. The plasma LncRNA LUCAT1 is positively associated with the disease activity in patients with CD, and it may act as a noninvasive biomarker to identify the degree of disease activity.
- Published
- 2021
- Full Text
- View/download PDF
3. Resource scarcity aggravates ingroup bias: Neural mechanisms and cross‐scenario validation
- Author
-
Fang Cui, Kexin Deng, Jie Liu, Xiaoxuan Huang, Jiamiao Yang, Yue‐jia Luo, Chunliang Feng, and Ruolei Gu
- Subjects
General Psychology - Published
- 2023
4. Characterization of chemical composition variations in raw and processed Codonopsis Radix by integrating metabolomics and glycomics based on multiple chromatography‐mass spectrometry technology
- Author
-
Yan, Wang, Jing-Jing, Zhang, Zi-Xia, Wang, Fang, Cui, Qian-Nian, Zhang, Ping-Ping, Song, Bing, Li, Zhuo-Shi, Tang, Fang-di, Hu, and Xiao-Feng, Shi
- Subjects
Chromatography ,Codonopsis ,Technology ,Metabolomics ,Filtration and Separation ,Glycosides ,Glycomics ,Chromatography, High Pressure Liquid ,Mass Spectrometry ,Drugs, Chinese Herbal ,Analytical Chemistry - Abstract
Codonopsis Radix, a popular food homology medicine, is widely used in clinical traditional Chinese medicine and food supplement, raw products and three types of processed products are the main forms of decoction pieces in China. However, there is no scientific basis for comprehensive chemical characterization of raw and three types of processed products. Herein, we investigated qualitatively and quantificationally secondary and primary metabolites in raw Codonopsis Radix and three types of processed products by metabolomics and glycomics employing multiple chromatography-mass spectrometry technology combined with chemometric analysis further to look for differential compounds and propose the processing-induced chemical mechanisms. The results indicated that Codonopsis Radix became dark-colored and the smell of burnt incense odor was observed after processing. The principal component analysis demonstrated that secondary metabolome and glycome were significantly altered between raw and processed products, and 36 differential secondary metabolites and 11 differential primary metabolites were finally screened through orthogonal partial least-squares-discriminant analysis. The main types of compounds are alkaloids, terpenoids, glycosides, amino acids, monosaccharides, oligosaccharides, and furfural derivatives. Meanwhile, Chemical mechanisms could be involved, including oxidation, glycosidic hydrolysis, esterification, dehydration, and Maillard reaction. This work supplies a chemical basis for the application of various types of Codonopsis Radix decoction pieces.
- Published
- 2022
5. Preparation facilitates response inhibition based on a stop‐signal task
- Author
-
Lu‐xia Jia, Shuang‐chen Li, Ji‐fang Cui, Jia‐li Liu, Xiao‐xiao Guo, and Ya Wang
- Subjects
General Psychology - Published
- 2023
6. Isoindolium‐Based Allenes: Reactivity Studies and Applications in Fluorescence Temperature Sensing and Cysteine Bioconjugation
- Author
-
Wa‐Yi O, Jian‐Fang Cui, Qiong Yu, Karen Ka‐Yan Kung, Sai‐Fung Chung, Yun‐Chung Leung, and Man‐Kin Wong
- Subjects
General Medicine ,General Chemistry ,Catalysis - Published
- 2023
7. Mental time travel for self and others in individuals with a high level of schizotypy
- Author
-
Jun‐yan Ye, Xiao‐jing Qin, Ji‐fang Cui, Lu‐xia Jia, Hai‐song Shi, Tian‐xiao Yang, Simon S. Y. Lui, Ya Wang, and Raymond C. K. Chan
- Subjects
General Psychology - Published
- 2023
8. Intermolecular Metal‐Free Cyclopropanation and Aziridination of Alkenes with XH 2 (X=N, C) by Thianthrenation**
- Author
-
Ming‐Shang Liu, Hai‐Wu Du, Jian‐Fang Cui, and Wei Shu
- Subjects
General Chemistry ,General Medicine ,Catalysis - Published
- 2022
9. Schizophrenia patients with poor clinical insight report less subjective memory problems
- Author
-
Ya Wang, Jun-yan Ye, Simon S.Y. Lui, Tian-xiao Yang, Xiao-jing Qin, Ji-fang Cui, Raymond C.K. Chan, Lu-xia Jia, Shuping Tan, Eric F.C. Cheung, and Ming-yuan Gan
- Subjects
Positive and Negative Syndrome Scale ,Working memory ,business.industry ,Subjective memory ,Neuropsychological Tests ,medicine.disease ,behavioral disciplines and activities ,030227 psychiatry ,03 medical and health sciences ,Memory, Short-Term ,0302 clinical medicine ,Visual memory ,Retrospective memory ,Schizophrenia ,Humans ,Medicine ,In patient ,Prospective Studies ,Verbal memory ,business ,030217 neurology & neurosurgery ,General Psychology ,Retrospective Studies ,Clinical psychology - Abstract
This study aimed to explore the relationships among clinical insight, subjective memory complaints, and objective memory performance in patients with schizophrenia. We recruited 205 patients with schizophrenia and 221 healthy controls in this study. The participants were administered a subjective-report scale on memory (the Prospective and Retrospective Memory Questionnaire), and several objective memory tasks measuring verbal memory, visual memory, and working memory. Clinical insight was measured with an item in the Positive and Negative Syndrome Scale. We found that when patients with schizophrenia were divided into subgroups with good and poor insight, both subgroups showed impairment in memory performance compared with controls. The schizophrenia patients with good insight reported similar memory complaints as controls whereas patients with poor insight reported less memory complaints than did the controls. These findings suggest that clinical insight may be related to subjective memory complaints, but not objective memory performance.
- Published
- 2021
10. Involuntary mental time travel in individuals with schizotypal personality features
- Author
-
Hai-song Shi, Ji-fang Cui, Tao Chen, Xiao-jing Qin, Ya Wang, Lu-xia Jia, and Lu-lu Liu
- Subjects
Adult ,Male ,Psychometrics ,Memory, Episodic ,media_common.quotation_subject ,Chronesthesia ,Emotions ,Structural validity ,Emotional intensity ,050105 experimental psychology ,Time ,Schizotypal Personality Disorder ,Free Association ,Young Adult ,03 medical and health sciences ,0302 clinical medicine ,Surveys and Questionnaires ,Humans ,Personality ,0501 psychology and cognitive sciences ,General Psychology ,Reliability (statistics) ,media_common ,05 social sciences ,Reproducibility of Results ,Translating ,Positive emotion ,Female ,Self Report ,Psychology ,030217 neurology & neurosurgery ,Clinical psychology - Abstract
Involuntary mental time travel (MTT) refers to the phenomenon of mentally reliving past experiences or pre-living possible future events in an involuntary form. Few studies have explored involuntary MTT in individuals with schizotypal personality features. The present study aimed to first explore the psychometric properties of the Involuntary Autobiographic Memory Inventory (IAMI) in a Chinese sample (Study 1), and then to explore whether individuals with schizotypal personality features experience involuntary MTT more frequently than individuals without schizotypal features. Moreover, the study explored whether the aberrant frequency of involuntary MTT is correlated with positive schizotypal features (Study 2). The results showed that the IAMI had good structural validity and reliability in a Chinese sample. Individuals with schizotypal traits reported a significantly higher frequency, less positive emotion, and stronger emotional intensity for both involuntary memories and future thoughts compared with individuals without schizotypal features. Further analyses in individuals with schizotypal personality features showed that the frequencies of both involuntary memories and future thoughts were significantly correlated with positive schizotypal traits. These results have potential theoretical and clinical implications for a comprehensive understanding of involuntary MTT among individuals with schizotypal personality features.
- Published
- 2019
11. Oxygen‐Generated Hierarchical‐Structured AuNRs@MnO 2 @SiO 2 Nanocarrier for Enhanced NIR‐ and H 2 O 2 ‐Responsive Mild‐Hyperthermia Photodynamic/Photothermal Combined Tumor Therapy
- Author
-
Wen‐Jie Zheng, Ze‐Lei Zhou, Qi‐Yan Lv, Xiaojie Song, Wen‐Xing Zhang, and Hui‐Fang Cui
- Subjects
Pharmacology ,Biochemistry (medical) ,Pharmaceutical Science ,Medicine (miscellaneous) ,Pharmacology (medical) ,Genetics (clinical) - Published
- 2022
12. Silver-Mediated Organic Transformations of Propargylamines to Enones, α-Thioketones, and Isochromans
- Author
-
Han Chen, Qiong Yu, Jian Fang Cui, Bin Yang, Nathanael Chun Him Lai, and Man-Kin Wong
- Subjects
Chemistry ,General Chemistry - Published
- 2019
13. Moral contagion: Devaluation effect of immorality on hypothetical judgments of economic value
- Author
-
Fang Cui, Juanzhi Lu, Jie Liu, Yuejia Luo, and Chong Liao
- Subjects
Adult ,Male ,Immorality ,Decision Making ,Ventromedial prefrontal cortex ,Devaluation ,Morals ,050105 experimental psychology ,Judgment ,Young Adult ,03 medical and health sciences ,0302 clinical medicine ,Phenomenon ,medicine ,Humans ,0501 psychology and cognitive sciences ,Radiology, Nuclear Medicine and imaging ,Valence (psychology) ,Research Articles ,Causal model ,Brain Mapping ,Radiological and Ultrasound Technology ,medicine.diagnostic_test ,Economics, Behavioral ,05 social sciences ,Brain ,Magnetic Resonance Imaging ,Economic valuation ,medicine.anatomical_structure ,Neurology ,Female ,Neurology (clinical) ,Anatomy ,Functional magnetic resonance imaging ,Psychology ,Social psychology ,Photic Stimulation ,Psychomotor Performance ,030217 neurology & neurosurgery - Abstract
Moral contagion is a phenomenon in which individuals or objects take on the moral essence of the people who are associated with them. Previous studies have found that individuals value objects associated with moral and likable people more than those associated with immoral and dislikable people. However, the neural mechanisms underlying this "moral contagion effect" have not yet been explored. In the present study, we combined a novel "Second-hand Goods Pricing" paradigm with functional magnetic resonance imaging to (a) confirm the existence of the moral contagion effect on the hypothetical economic valuation of objects and (b) determine the neural substrates underlying it. Participants were shown second-hand goods, information regarding the moral valence of the previous owner, and an initial price assigned to the object by computer. The participants were then asked to adjust the initial price to one they deemed most reasonable. Behaviorally, we found a significant devaluation effect for immoral owners and a weaker reverse effect for moral owners. Imaging data showed that the devaluation effect was primarily driven by neural responses in the dorsal striatum (mainly the caudate nucleus) that were triggered by high initial prices assigned to the "contaminated" objects. Dynamic causal modeling revealed that the high initial price assigned to "contaminated" objects led to increased effective connectivity from the caudate nucleus to the ventromedial prefrontal cortex-the brain area that integrates values during decision making.
- Published
- 2019
14. (2887) Proposal to conserve the name Prunus maackii against P . glandulifolia ( Rosaceae )
- Author
-
Bao‐Huan Wu, Daniel Potter, and Da‐Fang Cui
- Subjects
Plant Science ,Ecology, Evolution, Behavior and Systematics - Published
- 2022
15. Metal‐Free Cyclocarboamination of ortho ‐Formyl Phenylacetylenes with Secondary Amines: Access to 1,3‐Diamino‐1 H ‐Indenes and 3‐Amino‐1‐Indanones
- Author
-
Nathanael Chun Him Lai, Bin Yang, Jia-Jun Jiang, Rishi Tang, Man-Kin Wong, Jie-Ren Deng, and Jian Fang Cui
- Subjects
Metal free ,010405 organic chemistry ,Chemistry ,General Chemistry ,010402 general chemistry ,01 natural sciences ,Medicinal chemistry ,0104 chemical sciences - Published
- 2018
16. Moderating effect of age on the association between future time perspective and preventive coping
- Author
-
Lu-lu Liu, Tao Chen, Hai-song Shi, Xing-jie Chen, Ji-fang Cui, David Shum, Ya Wang, Raymond C.K. Chan, and David L. Neumann
- Subjects
Proactive coping ,Time perspective ,Coping (psychology) ,05 social sciences ,050109 social psychology ,0501 psychology and cognitive sciences ,Psychology ,Moderation ,050105 experimental psychology ,General Psychology ,Clinical psychology - Abstract
The present study aimed to investigate the moderating effect of age on the relationship between future time perspective (FTP) and future-oriented coping. A total of 1,915 participants aged 9-84 years completed measures of FTP and future-oriented coping. Moderation analyses were conducted to examine whether age played a role in the association between FTP and future-oriented coping (proactive and preventive). Results showed that proactive and preventive coping were negatively correlated with age, and age moderated the association between FTP and preventive coping but not proactive coping. Furthermore, the strength of the positive association between FTP and preventive coping was strongest among the older participants, moderate among the middle-aged participants, and weakest among the younger participants. These results suggest that the association between FTP and preventive coping varies across the lifespan.
- Published
- 2017
17. C,O-Chelated BINOL/Gold(III) Complexes: Synthesis and Catalysis with Tunable Product Profiles
- Author
-
Nathanael Chun Him Lai, Hok-Ming Ko, Jian Fang Cui, Ka-Pan Shing, Man-Kin Wong, and Jie-Ren Deng
- Subjects
Denticity ,010405 organic chemistry ,Chemistry ,Nanotechnology ,General Medicine ,General Chemistry ,010402 general chemistry ,Ring (chemistry) ,01 natural sciences ,Catalysis ,0104 chemical sciences ,Cycloisomerization ,Gold iii ,Polymer chemistry ,Chelation - Abstract
Unprecedented stable BINOL/gold(III) complexes, adopting a novel C,O-chelation mode, were synthesized by a modular approach through combination of 1,1'-binaphthalene-2,2'-diols (BINOLs) and cyclometalated gold(III) dichloride complexes [(C^N)AuCl2 ]. X-ray crystallographic analysis revealed that the bidentate BINOL ligands tautomerized and bonded to the AuIII atom through C,O-chelation to form a five-membered ring instead of the conventional O,O'-chelation giving a seven-membered ring. These gold(III) complexes catalyzed acetalization/cycloisomerization and carboalkoxylation of ortho-alkynylbenzaldehydes with trialkyl orthoformates.
- Published
- 2017
18. Developmental trajectory of time perspective: From children to older adults
- Author
-
Lu-lu Liu, Ji-fang Cui, Ya Wang, Xing-jie Chen, and Tao Chen
- Subjects
Time perspective ,Cross-sectional study ,05 social sciences ,Perspective (graphical) ,050109 social psychology ,050105 experimental psychology ,Developmental psychology ,Economic situation ,Developmental trajectory ,Multiple time dimensions ,0501 psychology and cognitive sciences ,Young adult ,Psychology ,Construct (philosophy) ,General Psychology - Abstract
Time perspective is a fundamental dimension of the psychological time construct, with a pervasive and powerful influence on human behavior. However, the developmental trajectory of time perspective across a human lifespan remains unclear. The current study aimed to portray the developmental trajectory of all dimensions of time perspectives from children to older adults in a large sample. A total of 1,901 individuals (aged 9-84 years) completed measures of time perspective. They were then divided into five age groups: children, teenagers, young adults, middle-aged adults, and older adults. Results suggested that each time perspective showed a unique developmental pattern across the lifespan. Moreover, perceived economic situation and education were related to some dimensions of time perspective.
- Published
- 2016
19. Testing the Zimbardo Time Perspective Inventory in the Chinese context
- Author
-
Lu-lu Liu, Xing-jie Chen, Ya Wang, and Ji-fang Cui
- Subjects
Time perspective ,Chinese version ,Internal consistency ,Context (language use) ,Set (psychology) ,Psychology ,Social psychology ,General Psychology ,Reliability (statistics) ,Developmental psychology - Abstract
In this study, the authors evaluated the Chinese version of the Zimbardo Time Perspective Inventory (ZTPI). The ZTPI was tested among a sample of 303 university students. A subsample of 51 participants was then asked to complete the ZTPI again along with another set of questionnaires. The five-factor model of a 20-item short version of the ZTPI showed good model fit, internal consistency, and test-retest reliability. The 20-item Chinese version of the ZTPI also provided good validity, showing correlations with other variables in expected directions. Past-Positive was positively correlated with reappraisal and negatively correlated with suppression emotion regulation strategies, and Present-Hedonistic was positively correlated with reappraisal emotion regulation strategies. These findings indicate that the ZTPI is a reliable and valid instrument for measuring time perspective in the Chinese setting.
- Published
- 2015
20. One Step Preparation of Reduced Graphene Oxide/Pd-Fe3O4@Polypyrrole Composites and Their Application in Catalysis
- Author
-
Hao Wang, Xiao Zhang, Tongjie Yao, Jie Wu, Tieyu Cui, Quan Zuo, and Fang Cui
- Subjects
Graphene ,Organic Chemistry ,Oxide ,Nanoparticle ,chemistry.chemical_element ,Nanotechnology ,General Chemistry ,Heterogeneous catalysis ,Polypyrrole ,Biochemistry ,Catalysis ,law.invention ,chemistry.chemical_compound ,chemistry ,Chemical engineering ,law ,Nanosheet ,Palladium - Abstract
The simple preparation of catalysts with superior catalytic activity and good reusability is highly desirable. Herein, we report a novel strategy to construct reduced graphene oxide (rGO)/Pd-Fe3 O4 @polypyrrole (PPy) catalysts with Pd and Fe3 O4 nanoparticles anchored on a rGO nanosheet surface and wrapped in a PPy shell. The synthesis and assembly of both the Pd and Fe3 O4 nanoparticles, the preparation of the PPy layer, and the reduction of graphene oxide nanosheets were finished in one step. In the system, the PPy layer not only prevented aggregation of Pd and Fe3 O4 nanoparticles, but also generated a synergistic effect with precursor Pd(2+) ions, which led to a high dispersity of as-prepared Pd nanoparticles. Although the procedure was simplified to one step, the catalytic activity and reusability were not sacrificed. In the reduction of 4-nitrophenol, their catalytic performance was better than that in recent reports. Moreover, the catalysts showed good reusability owing to their magnetic properties.
- Published
- 2015
21. Photooxidative Amidation of Aldehydes with Amines Catalyzed by Rose Bengal
- Author
-
Man-Kin Wong, Jian Fang Cui, Karen Ka Yan Kung, Franco King Chi Leung, and Tze Wai Hui
- Subjects
Reaction conditions ,chemistry.chemical_compound ,chemistry ,Organic Chemistry ,Rose bengal ,Photocatalysis ,Organic chemistry ,Organic synthesis ,Chemoselectivity ,Catalysis - Abstract
An efficient method for photooxidative amidation of aldehydes with secondary amines has been developed. With the use of a readily available photocatalyst rose bengal, tertiary amides (with examples of oligosaccharides and endoperoxide artemisinin) were obtained in good yields with high chemoselectivity and functional-group compatibility under mild reaction conditions.
- Published
- 2015
22. Three New Phthalides fromGnaphalium adnatum
- Author
-
Liang Li, Jing-Feng Zhao, Hongbin Zhang, Xing-Ping Zheng, Xiao-Dong Yang, Li-Juan Yang, and Qiong-Fang Cui
- Subjects
Inorganic Chemistry ,Gnaphalium ,biology ,Stereochemistry ,Genus ,Chemistry ,Organic Chemistry ,Drug Discovery ,Physical and Theoretical Chemistry ,Gnaphalium adnatum ,biology.organism_classification ,Biochemistry ,Catalysis - Abstract
Three new phthalides, gnaphalides A–C (1–3, resp.), together with three known phthalides, were isolated from the aerial part of Gnaphalium adnatum. The structures of the new compounds were elucidated as 6-(1,1-dimethylprop-2-en-1-yl)-5,7-dihydroxy-2-benzofuran-1(3H)-one (1), 5-hydroxy-7-[(2-hydroxy-3-methylbut-3-en-1-yl)oxy]-2-benzofuran-1(3H)-one (2), and 1,3-dihydro-7-[(3-methylbut-2-en-1-yl)oxy]-1-oxo-2-benzofuran-5-yl β-D-glucopyranoside (3) on the basis of spectral analyses. The structure of 1 was also confirmed by X-ray crystallographic analysis. The three known phthalides, identified as 5,7-dihydroxyisobenzofuran-1(3H)-one (4), anaphatol (5), and 7-O-(β-glucopyranosyl)-5-hydroxyisobenzofuran-1(3H)-one (6), were isolated from the genus Gnaphalium for the first time.
- Published
- 2014
23. Silver-Catalyzed Transformation of Propargylic AmineN-Oxides to Enones and Acyloxy KetonesviaIsoxazolinium Intermediates
- Author
-
Hok Ming Ko, Tsz Wai Hui, Jian Fang Cui, Man-Kin Wong, and Karen Ka Yan Kung
- Subjects
Transformation (genetics) ,Peptide modification ,Chemistry ,Propargyl ,Organic chemistry ,Amine gas treating ,General Chemistry ,Catalysis - Abstract
The propargylic amine N-oxides are formed in situ by oxygenation of the corresponding propargyl amines.
- Published
- 2014
24. ChemInform Abstract: Utilization of LDH-Based Materials as Potential Adsorbents and Photocatalysts for the Decontamination of Dyes Wastewater: A Review
- Author
-
Hou Wang, Zhigang Yu, Xiaofei Tan, Chang Zhang, Fenghua Wang, Guangming Zeng, Yu Zhong, Zhongzhu Yang, and Fang Cui
- Subjects
Aqueous solution ,Adsorption ,Chemical engineering ,Wastewater ,Environmental remediation ,Chemistry ,Photocatalysis ,Layered double hydroxides ,engineering ,General Medicine ,Human decontamination ,engineering.material ,Catalysis - Abstract
Dye as a colored organic pollution has caused tremendous environmental problems. Removing dyes from effluents is of significant importance. Layered double hydroxides (LDHs), known as hydrotalcite-like compounds or ionic lamellar compounds, have attracted considerable attention recently due to the presence of large interlayer spaces, positively charged layers and solvation molecules. LDH-based materials are frequently used for environmental remediation especially for the elimination of dye compounds. This review compiles an extensive list of LDH-based low-cost sorbents and photocatalytic catalysts for dyes from the vast literature. Furthermore, performance, key factors and mechanisms involved in the processes are also included. Lastly, some major challenges together with prospects in this research field are discussed and highlighted. In conclusion, the application of LDH-based materials in the area of adsorption and catalysis science represents a useful and viable method, resulting in the outstanding capabilities in the removal of dyes from aqueous systems.
- Published
- 2016
25. Coexistence of peripheral myelin protein 22 and dystrophin mutations in a chinese boy
- Author
-
Fang Cui, Xusheng Huang, Fei Yang, Honghu Wu, Chuanqiang Pu, Zhanjun Wang, Dajun Chen, and Zhaohui Chen
- Subjects
musculoskeletal diseases ,Genetics ,congenital, hereditary, and neonatal diseases and abnormalities ,Weakness ,Pes cavus ,Pathology ,medicine.medical_specialty ,Muscle biopsy ,Proximal muscle weakness ,medicine.diagnostic_test ,biology ,Physiology ,Duchenne muscular dystrophy ,medicine.disease ,nervous system diseases ,Cellular and Molecular Neuroscience ,Exon ,Physiology (medical) ,Peripheral myelin protein 22 ,medicine ,biology.protein ,Neurology (clinical) ,medicine.symptom ,Dystrophin - Abstract
Introduction: We describe a 10-year-old Chinese boy with features of Charcot–Marie–Tooth disease (CMT) and Duchenne muscular dystrophy (DMD). Methods: Case report. Results: Weakness and mild sensory loss in the distal extremities, pes cavus, and nerve conduction findings suggested demyelinating neuropathy, while moderate calf pseudohypertrophy, proximal muscle weakness, a myopathic pattern on electromyography, and deficiency of dystrophin immunohistochemical staining on muscle biopsy indicated DMD. Genetic testing revealed a large deletion spanning exon 50 in the gene coding for dystrophin and duplications in the gene coding for peripheral myelin protein 22. Conclusions: This is an interesting and very rare case of CMT type 1A comorbid with DMD. This results in an unusual phenotype and rapid deterioration of motor function. Usage of both target region capture and next generation sequencing is a powerful tool for predicting precisely the range of the large DNA fragment deletion in DMD. Muscle Nerve 48: 979–983, 2013
- Published
- 2013
26. 2,3,10,11-Tetrahydroxytetraphenylene and Its Application in Molecular Recognition
- Author
-
Jian Fang Cui, Zongwei Cai, Jianwei Han, Henry N. C. Wong, Xiang Gao, and Chao Chen
- Subjects
Inorganic Chemistry ,chemistry.chemical_compound ,Molecular recognition ,chemistry ,Stereochemistry ,Organic Chemistry ,Drug Discovery ,Tetraphenylene ,Physical and Theoretical Chemistry ,Biochemistry ,Catalysis ,Derivative (chemistry) - Abstract
A rationally designed 2,3,10,11-tetrahydroxytetraphenylene (1) has been synthesized. Employing 1 as a building block, a structurally novel tweezer-like host 2 containing dibenzo-24-crown-8 moieties has been prepared. Host 2 showed excellent molecular-recognition ability toward paraquat (=1,1′-dimethyl-4,4′-bipyridinium dichloride) derivative 3a to form a 1 : 1 stable complex in solution.
- Published
- 2012
27. Reversal Effect of Melanoma Differentiation Associated Gene-7/Interleukin-24 on Multidrug Resistance in Human Hepatocellular Carcinoma Cells
- Author
-
Chang-Rui Ding, Ping Fang, Xin Zhang, Shunchang Jiao, Yan Gao, and Fang Cui
- Subjects
Carcinoma, Hepatocellular ,Histology ,Biology ,Flow cytometry ,Downregulation and upregulation ,Cell Line, Tumor ,Interleukin 24 ,medicine ,Humans ,Doxorubicin ,Melanoma ,Ecology, Evolution, Behavior and Systematics ,medicine.diagnostic_test ,Cell growth ,Interleukins ,Liver Neoplasms ,Cell Differentiation ,Molecular biology ,Drug Resistance, Multiple ,Multiple drug resistance ,Treatment Outcome ,Apoptosis ,Cell culture ,Fluorouracil ,Anatomy ,Biotechnology ,medicine.drug - Abstract
Multidrug resistance is the main cause for failure of chemotherapy. Melanoma differentiation associated gene-7/interleukin-24 (mda-7/IL-24) has been implicated in the inhibition of human tumor cell proliferation. However, the reversing effect of mda-7/IL-24 on multidrug resistance of human hepatocellular carcinoma (HCC) is not fully clear. In this study, we investigated the effects of overexpression of the mda-7/IL-24 gene in human HCC. We established mda-7/IL-24 overexpressing BEL-7402/5-fluorouracil (5-FU) cell lines and their drug sensitivity to 5-FU and doxorubicin (DOX) which were investigated by MTT. Furthermore, we investigated the apoptotic rate and the intracellular accumulation of Rhodamine-123 and DOX by flow cytometry. We also studied the expression of multidrug resistance gene 1 (MDR1), lung resistance-related protein (LRP), and multidrug resistance-related protein 1 (MRP1) by real-time polymerase chain reaction and Western blotting. Transcriptional activation of AP-1 and NF-κB was determined by luciferase reporter assay. The drug sensitivity of 5-FU or DOX, the apoptotic rate, and the intracellular accumulation of Rhodamine-123 and DOX were increased, while the mRNA and protein expression levels of MDR1, LRP, and MRP1 were reduced. The transcriptional activation of AP-1 and NF-κB was suppressed in mda-7/IL-24 overexpressing BEL-7402/5-FU cells. Our results demonstrated that mda-7/IL-24 could restore the drug sensitivity through the downregulation of MDR1, MRP1, and LRP expression, as well as the transcriptional activation of AP-1 and NF-κB and effectively reverse MDR.
- Published
- 2012
28. The apoptotic effect and associated signalling of HSP90 inhibitor 17-DMAG in hepatocellular carcinoma cells
- Author
-
Xiu‑hua Li, Guiying Zhang, Ting Xiong, Ai‑min Leng, Yuxiang Chen, Ting Liu, Ya‑nan Zhu, Jian‑fang Cui, and Jing Yang
- Subjects
Carcinoma, Hepatocellular ,Lactams, Macrocyclic ,Survivin ,Antineoplastic Agents ,Apoptosis ,Biology ,Inhibitor of Apoptosis Proteins ,Hsp90 inhibitor ,chemistry.chemical_compound ,Cyclin D1 ,Annexin ,Cell Line, Tumor ,Benzoquinones ,medicine ,Humans ,HSP90 Heat-Shock Proteins ,Propidium iodide ,Cell Proliferation ,Cisplatin ,Liver Neoplasms ,NF-kappa B ,Cell Biology ,General Medicine ,Geldanamycin ,Molecular biology ,chemistry ,Cancer research ,Tumor Suppressor Protein p53 ,medicine.drug - Abstract
Primary liver cancer is one of the highly malignant tumours. The traditional surgery, chemotherapy and radiation therapy only established 6% of 5-year survival rate in HCC (hepatocellular carcinoma). Therefore there is an urgent need to develop new therapeutic strategies. HSP90 (heat shock protein 90) is one of the important molecular chaperones and was identified with high expression in the primary liver cancer. In this study, we evaluated the therapeutic effect of specific HSP90 inhibitor 17-DMAG (17-dimethylaminoethylamino-17-demethoxy geldanamycin) in HCC cells. The time and concentration effects of 17-DMAG were investigated in HCC cells. Cell proliferation was measured by MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide] assay and cell counting. Apoptosis was detected by flow cytometry with staining of Annexin V-FITC/PI (propidium iodide). The protein levels of survivin, cyclin D1, p53 and NF-κB (nuclear factor κB) were measured by Western blotting. 17-DMAG inhibited the proliferation of HCC cells in a time- and concentration-dependent manner. Treatment with 400 nmol/l 17-DMAG for 48 h significantly induced early-stage apoptosis (22.4%). Conversely, it induced less late-stage apoptosis (3.03%). The 5 mg/l of cisplatin induced significantly less early-stage apoptosis (6.5%), but similar proportion of late-stage apoptosis (4.89%) compared with 17-DMAG. Inhibition of HSP90 activity by 400 nmol/l 17-DMAG decreased protein levels of survivin, cyclin D1 and NF-κB protein levels, whereas increased p53 protein level. HSP90 plays a key role in HCC cell growth and survival through regulation of survivin, cyclin D1, p53 and nucleus NF-κB protein levels and the specific HSP90 inhibitor 17-DMAG can play a therapeutic role in HCC treatment.
- Published
- 2012
29. Liquid Phase Hydrogenolysis of Biomass-derived Lactate to 1,2-Propanediol over Silica Supported Cobalt Nanocatalyst
- Author
-
Fang Cui, Jingjing Xue, Zhiwei Huang, Chungu Xia, Jianliang Zuo, and Jing Chen
- Subjects
inorganic chemicals ,Chemistry ,Inorganic chemistry ,chemistry.chemical_element ,General Chemistry ,Olation ,Catalysis ,law.invention ,Propanediol ,chemistry.chemical_compound ,law ,Hydrogenolysis ,Ethyl lactate ,Calcination ,Dispersion (chemistry) ,Cobalt - Abstract
Liquid phase hydrogenolysis of ethyl lactate to 1,2-propanediol was performed over silica supporting cobalt catalysts prepared by two different methods: precipitation-gel (PG) technique and deposition-precipitation (DP) procedure. The cobalt species (Co3O4/cobalt phyllosilicate) present in the corresponding calcined PG and DP catalysts were different as a consequence of the preparation methods, and CoOHCo olation and SiOCo oxolation molecular mechanisms were employed to elucidate the chemical phenomena during the different preparation procedures. In addition, the texture (BET), reduction behavior (TPR and in-situ XRD), surface dispersion and state of cobalt species (XPS), and catalytic performance differ greatly between the samples. Because of small particle size, high dispersion of cobalt species and facile reducibility, the Co/SiO2 catalyst prepared by precipitation-gel method presented a much higher activity than the catalyst prepared by deposition-precipitation method. Metallic cobalt is assumed to be the catalytically active site for the hydrogenolysis reaction according to the catalytic results of both cobalt samples reduced at different temperatures and the structure changes after reaction.
- Published
- 2011
30. Chiritopsis danxiaensis sp. nov. (Gesneriaceae) from Mount Danxiashan, south China
- Author
-
Shi-Shi Lin, Wenbo Liao, Ru-jiang Shen, Da-Fang Cui, and Yi Yu
- Subjects
Bract ,South china ,Genus ,Chiritopsis danxiaensis ,Botany ,Chiritopsis ,Key (lock) ,Plant Science ,Biology ,biology.organism_classification ,Gesneriaceae ,Ecology, Evolution, Behavior and Systematics - Abstract
A new species of Chiritopsis (Gesneriaceae), C. danxiaensis W. B. Liao, S. S. Lin & R. J. Shen, is described and illustrated from Mount Danxiashan, Guangdong Province, China. The new species is morphologically closely related to C. lobulata and C. cordifolia, but is characterized by having denticulate bracts, yellowish corolla, 2 glabrous staminodes and longer style. A revised key to all species of the genus is also provided.
- Published
- 2010
31. Development of High Performance Liquid Chromatography Method for Costunolide and Dehydrocostuslactone in Mice Plasma and Tissues: Application to Pharmacokinetic Study
- Author
-
Yuqiong Wu, Fang Cui, Shilan Feng, Yongjie Li, Chunming Wang, Fangdi Hu, and Yingyan Bi
- Subjects
Costunolide ,chemistry.chemical_compound ,Chromatography ,Uv detector ,Pharmacokinetics ,Chemistry ,Extraction (chemistry) ,General Chemistry ,Mice plasma ,High-performance liquid chromatography - Abstract
A simple and sensitive high performance liquid chromatographic (HPLC) method for simultaneous determination of costunolide and dehydrocostuslactone in mice plasma and tissues was developed and validated. Costunolide and dehydrocostuslactone were extracted into acetonitrile and separated using an isocratic mobile phase, on a Hypersil ODS C18 column. The effluent was monitored by UV detector at 210 nm and at a flow rate of 1.0 mL.min (1) and 25 degrees C. The linearity ranges of proposed method were 0.223-8.920 mu g.mL (1) for costunolide and 0.227-9.080 mu g.mL (1) for dehydrocostuslactone. The intra-day and inter-day RSD of the assay method for the two components were less than 5%, and mean recovery was within the 86.5% to 101.8% range. The method was found to be precise, accurate, and specific during the study. The method was successfully applied for pharmacokinetic study of costunolide and dehydrocostuslactone after application of ethanol extraction of Muxiang (EEM) in mice.
- Published
- 2010
32. The up-regulation of 14-3-3 proteins in Smad4 deficient epidermis and hair follicles at catagen
- Author
-
Linyi Qian, Jinfeng Liu, Liyan Jiao, Wantao Ying, Xiaohong Qian, Lei Li, Haiying Hang, Fang Cui, Zhishang Hu, Cunzhong Yuan, Xiao Yang, Leilei Yang, and Yan Teng
- Subjects
Keratinocytes ,Spectrometry, Mass, Electrospray Ionization ,Difference gel electrophoresis ,Mutant ,Biology ,Models, Biological ,Biochemistry ,Mice ,Downregulation and upregulation ,Keratin ,medicine ,Animals ,Databases, Protein ,Molecular Biology ,Smad4 Protein ,Mice, Knockout ,chemistry.chemical_classification ,Regulation of gene expression ,integumentary system ,Epidermis (botany) ,Alopecia ,Hair follicle ,Molecular biology ,Gene Expression Regulation, Neoplastic ,medicine.anatomical_structure ,14-3-3 Proteins ,chemistry ,Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ,Epidermis ,Isoelectric Focusing ,Keratinocyte ,Hair Follicle - Abstract
Each postnatal hair follicle (HF) perpetually goes through three phases: anagen, catagen, and telogen. The molecular signals that orchestrate the follicular transition between phases are still largely unknown. Our previous study shows that the keratinocyte specific Smad4 knockout mice exhibit progressive alopecia due to the mutant HFs failure to undergo programmed regression. To investigate the detailed molecular events controlling this process, the protein profiles of Smad4 mutant and control epidermal and HF keratinocytes were compared using 2-D difference gel electrophoresis (2-D DIGE) proteomic analysis. Eighty-six differentially expressed protein spots were identified by MALDI-TOF/TOF MS or ESI-MS/MS as 72 proteins, of which 29 proteins were found to be changed during the anagen-catagen transition of HFs in Smad4 mutants compared with the controls. The differentially expressed proteins represent a wide spectrum of functional classes such as keratin, the cytoskeleton, cellular growth and differentiation, ion combination and transfer, protein enzymes. Notably, we found that the 14-3-3sigma protein together with the 14-3-3zeta and 14-3-3beta proteins were significantly down-regulated only in wild-type keratinocytes but not in Smad4 mutant keratinocytes during the catagen phase, suggesting that increased expression of 14-3-3 proteins might contribute to the blockade of catagen initiation in Smad4 deficient HFs.
- Published
- 2008
33. Correlation between myocardial deformation on three-dimensional speckle tracking echocardiography and cardiopulmonary exercise testing
- Author
-
Li, Mei, primary, Lu, Yaran, additional, Fang, Cui, additional, and Zhang, Xiaorong, additional
- Published
- 2017
- Full Text
- View/download PDF
34. Reactive oxygen species, but not mitochondrial membrane potential, is associated with radiation-induced apoptosis of AHH-1 human lymphoblastoid cells
- Author
-
Yu-Qing Liu, Zhu-jun Jiang, Yu-Fang Cui, Xiao-xia An, Yan Li, Ying Guo, Wei Jin, Han Xu, Ying Zhang, and Zi-Bing Wang
- Subjects
Membrane Potential, Mitochondrial ,chemistry.chemical_classification ,Reactive oxygen species ,Cell growth ,Lymphoblast ,Apoptosis ,Cell Biology ,General Medicine ,Biology ,Mitochondrion ,Rhodamine 123 ,Mitochondria ,Cell biology ,chemistry.chemical_compound ,chemistry ,Gamma Rays ,Caspases ,Cell Line, Tumor ,Humans ,Lymphocytes ,Viability assay ,Reactive Oxygen Species ,Intracellular ,Cell Proliferation - Abstract
The aim of the study was to investigate the sensitivity of AHH-1 human lymphoblastoid cells to radiation and its relevance to intracellular events, specifically alteration in cellular energy-producing systems. AHH-1 human lymphoblastoid cells were irradiated with 6 Gy of gamma radiation, and then were collected at the indicated time points. Parallel studies were conducted to assess the effects of radiation on the cell proliferation and apoptotic index. Mitochondrial membrane potential (MMP) and reactive oxygen species (ROS) production were monitored. A marked decrease of cell viability was observed as early as 12 h postirradiation and fraction of apoptotic cells was highest at 24 h. Intracellular ROS generation measured with 2',7'-dichlorodihydrofluorescein diacetate (DCFH-DA) appeared to be highest as early as 30 min postirradiation and resumed to normal level at 6 h. Unexpectedly, the fluorescence intensity of Rhodamine 123 for measuring MMP did not change during the first 3h after radiation and exhibited an aberrant increase at 6 h. The results suggest that AHH-1 cells are sensitive to radiation-induced apoptosis and ROS generation is an early phase in the apoptosis process. Moreover, the results might cast doubts on those studies using Rhodamine 123 which hypothesized that the fall in MMP is one of the early events of apoptosis.
- Published
- 2007
35. ChemInform Abstract: Three New Phthalides from Gnaphalium adnatum
- Author
-
Jing-Feng Zhao, Hongbin Zhang, Xing-Ping Zheng, Li-Juan Yang, Xiao-Dong Yang, Qiong-Fang Cui, and Liang Li
- Subjects
Antitumor activity ,Stereochemistry ,Chemistry ,General Medicine ,Gnaphalium adnatum - Abstract
isolation, structure elucidation, and antitumor activity of new phthalides, gnaphalides A-C (I)-(III)
- Published
- 2015
36. ChemInform Abstract: Silver-Catalyzed Transformation of Propargylic Amine N-Oxides to Enones and Acyloxy Ketones via Isoxazolinium Intermediates
- Author
-
Karen Ka Yan Kung, Tsz Wai Hui, Jian Fang Cui, Man-Kin Wong, and Hok Ming Ko
- Subjects
Transformation (genetics) ,Chemistry ,Propargyl ,Amine gas treating ,General Medicine ,Medicinal chemistry ,Catalysis - Abstract
The propargylic amine N-oxides are formed in situ by oxygenation of the corresponding propargyl amines.
- Published
- 2015
37. The up-regulation of proteasome subunits and lysosomal proteases in hepatocellular carcinomas of theHBx gene knockin transgenic mice
- Author
-
Youliang Wang, Jingqun Hu, Jie Wang, Xiaohang Zhao, Kaihua Wei, Fang Liu, Xiao Yang, Fang Cui, Hongli Wang, and Xuemin Zhang
- Subjects
Cyclin-Dependent Kinase Inhibitor p21 ,Male ,Proteomics ,Genetically modified mouse ,Hepatitis B virus ,Proteasome Endopeptidase Complex ,Carcinoma, Hepatocellular ,PSMC2 ,viruses ,Transgene ,Mice, Transgenic ,Biology ,Biochemistry ,Cathepsin B ,Mice ,Hepatitis B, Chronic ,Gene knockin ,Animals ,Humans ,Electrophoresis, Gel, Two-Dimensional ,Viral Regulatory and Accessory Proteins ,Molecular Biology ,Reverse Transcriptase Polymerase Chain Reaction ,Liver Neoplasms ,Middle Aged ,HCCS ,PSMB4 ,Molecular biology ,digestive system diseases ,Up-Regulation ,Gene Expression Regulation, Neoplastic ,HBx ,Liver ,Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ,Trans-Activators ,Cancer research ,Lysosomes ,Peptide Hydrolases - Abstract
Chronic infection of hepatitis virus B (HBV) has been proven to be one of the most important risk factors of hepatocellular carcinoma (HCC). HBx has been shown to function in the viral life cycle and the development of HCC. Recently, we have reported that HBx transgenic mice (p21-HBx), generated by gene knockin, develop HCC at the age of 18 months. To further study the function of HBx during the development of HCC in vivo, we performed proteomic analysis of the transgenic and wild-type control mice. The combination of 2-DE and MALDI-TOF MS revealed that proteasome subunits (PSMA6, PSMB4, PSMC2 and PSMD12) were up-regulated in tumor tissues of the p21-HBx transgenic mice. Cathepsin B, ubiquinol-cytochrome C reductase core protein 1 and an ATP-dependent caseinolytic protease, which were involved in the cellular proteolytic process, were also found increased in tumors. The results were confirmed in tumors of transgenic mice and HCCs of human using RT-PCR. All these results suggested that the strengthened ubiquitin-proteasome and lysosomal pathway might contribute to the development of HBx-related HCC.
- Published
- 2006
38. Pathways to caspase activation
- Author
-
Yu-Fang Cui, Yu-Qing Liu, and Zi-Bing Wang
- Subjects
Programmed cell death ,Caspase 2 ,Apoptosis ,Endoplasmic Reticulum ,Granzymes ,Receptors, Tumor Necrosis Factor ,Animals ,Humans ,Caspase ,Tissue homeostasis ,biology ,Chemistry ,NLRP1 ,Serine Endopeptidases ,Intrinsic apoptosis ,Intracellular Signaling Peptides and Proteins ,Cytochromes c ,Proteins ,Cell Biology ,General Medicine ,Mitochondria ,Cell biology ,Enzyme Activation ,Apoptotic Protease-Activating Factor 1 ,Caspases ,biology.protein ,Poly(ADP-ribose) Polymerases ,Signal transduction ,Signal Transduction - Abstract
Apoptosis or programmed cell death is an active form of cell death which is essential for tissue homeostasis. Many proteins are involved in the molecular signal transduction of apoptosis. The caspase enzymes, a family of specific cysteine proteases, play a central role in cell death machinery. In this review, we mainly discuss the current understanding of several pathways to activate caspases and some key proteins related to these pathways.
- Published
- 2005
39. Preparation and Characterization of Aligned Carbon Nanotube-Ruthenium Oxide Nanocomposites for Supercapacitors
- Author
-
Hui-Fang Cui, Xiao Liu, Jian-Shan Ye, Wei-De Zhang, Tit Meng Lim, and Fwu-Shan Sheu
- Subjects
Nanotube ,Materials science ,Scanning electron microscope ,Oxide ,Carbon nanotube ,Chemical vapor deposition ,Ruthenium oxide ,Nanocomposites ,law.invention ,Biomaterials ,Magnetics ,chemistry.chemical_compound ,Microscopy, Electron, Transmission ,X-Ray Diffraction ,law ,Electrochemistry ,Nanotechnology ,General Materials Science ,Composite material ,Electrodes ,Nanotubes ,Nanocomposite ,Nanotubes, Carbon ,Nanowires ,Oxides ,General Chemistry ,Chemical engineering ,chemistry ,Microscopy, Electron, Scanning ,Nanoparticles ,Ruthenium Compounds ,Cyclic voltammetry ,Biotechnology - Abstract
A novel type of ruthenium oxide (RuO(2))-modified multi-walled carbon nanotube (MWNT) nanocomposite electrode (RuO(2)/MWNT) for supercapacitors has been prepared. The nanocomposites were formed by depositing Ru by magnetic-sputtering in an Ar/O(2) atmosphere onto MWNTs, which were synthesized on Ta plates by chemical vapor deposition. Cyclic voltammetry, chronopotentiometry, and electrochemical impedance measurements were applied to investigate the performance of the RuO(2)/MWNT nanocomposite electrodes. The capacitance of the MWNT electrodes in 1.0 M H(2)SO(4) is significantly increased from 0.35 to 16.94 mF cm(-2) by modification with RuO(2). The RuO(2) film on the surface of the nanotubes is composed of small crystal grains with tilted bundle-like microstructures, as observed by transmission electron microscopy (TEM) and scanning electron microscopy (SEM). The results demonstrate a promising route to prepare RuO(2)/MWNT-based double-layer supercapacitors.
- Published
- 2005
40. Gold-Cluster Sensors Formed Electrochemically at Boron-Doped-Diamond Electrodes: Detection of Dopamine in the Presence of Ascorbic Acid and Thiols
- Author
-
Qiqing Zhang, Jian Weng, Fwu-Shan Sheu, Hui-Fang Cui, Jian-Shan Ye, John Wang, and Junmin Xue
- Subjects
Detection limit ,Gold cluster ,Materials science ,Inorganic chemistry ,Diamond ,engineering.material ,Condensed Matter Physics ,Ascorbic acid ,Electrochemistry ,Electronic, Optical and Magnetic Materials ,Biomaterials ,Microelectrode ,Electrode ,Monolayer ,engineering - Abstract
Gold clusters have been electrodeposited on a boron-doped diamond (BDD) electrode by scanning the potential from 0.7 V to 0.0 V (vs. 3 M KCl-Ag/AgCl reference) in a solution of 0.5 mM KAuCl 4 and 1.0 M KCl. The cluster-modified diamond (Au/ BDD) electrode has been used to investigate the oxidative properties of dopamine (DA) and ascorbate (AA). The modified diamond electrode shows a higher activity for DA oxidation than AA; the oxidation potential of DA shifted to a less-positive potential (0.11 V) than that of AA, which oxidized at 0.26 V, and DA possesses a much higher peak current than that of AA. The reversibility of the electrode reaction with DA is significantly improved at the Au/BDD electrode, which results in a large increase in the square-wave voltammetric peak current, with a detection limit of 0.1 μM in the presence of a large excess of AA. The Au/BDD electrode shows excellent sensitivity and good selectivity for DA detection. A self-assembled monolayer (SAM) of mercaptoacetic acid on the Au clusters was used to provide an antifouling effect as the negative CO 2 - groups repulse negative ascorbate and attract positive dopamine in pH 7.4 buffer. After pre-absorption, the SAM/Au/BDD electrode could detect 1.0 nM DA in a linear range from 10 nM to 10 μM in the presence of 10 - 4 M AA.
- Published
- 2005
41. Electrochemical Biosensing Platforms Using Phthalocyanine-Functionalized Carbon Nanotube Electrode
- Author
-
Guo Qin Xu, Jian-Shan Ye, Hui-Fang Cui, Fwu-Shan Sheu, Wei De Zhang, and Ying Wen
- Subjects
Materials science ,biology ,Nanotechnology ,Carbon nanotube ,Electrocatalyst ,Analytical Chemistry ,Catalysis ,law.invention ,chemistry.chemical_compound ,chemistry ,Chemical engineering ,law ,Electrode ,Electrochemistry ,Phthalocyanine ,biology.protein ,Surface modification ,Glucose oxidase ,Biosensor - Abstract
Iron-phthalocyanines (FePc) are functionalized at multi-walled carbon nanotubes (MWNTs) to remarkably improve the sensitivity toward hydrogen peroxide. We constructed a highly sensitive and selective glucose sensor on FePcMWNTs electrode based on the immobilization of glucose oxidase (GOD) on poly-o-aminophenol (POAP)electropolymerized electrode surface. SEM images indicate that GOD enzymes trapped in POAP film tend to deposit primarily on the curved tips and evenly disperse along the sidewalls. The resulting GOD @ POAP/FePc-MWNTs biosensor exhibits excellent performance for glucose with a rapid response (less than 8 s), a wide linear range (up to 4.0 � 10 � 3 M), low detection limits (2.0 � 10 � 7 M with a signal-to-noise of 3), a highly reproducible response (RSD of 2.6%), and long-term stability (120 days). Such characteristics may be attributed to the catalytic activity of FePc and carbon nanotube, permselectivity of POAP film, as well as the large surface area of carbon nanotube materials.
- Published
- 2005
42. Thermosensitive poly(N-isopropylacrylamide) hydrogel for refolding of recombinant bovine prethrombin-2 fromE. coli inclusion bodies
- Author
-
Yi-Xin Guan, Zhi‐Fang Cui, Shan-Jing Yao, and Jian‐Liang Chen
- Subjects
chemistry.chemical_classification ,Chromatography ,Polymers and Plastics ,General Chemistry ,Polymer ,Lower critical solution temperature ,Surfaces, Coatings and Films ,Hydrophobic effect ,chemistry.chemical_compound ,chemistry ,Chemical engineering ,Self-healing hydrogels ,Materials Chemistry ,Side chain ,Poly(N-isopropylacrylamide) ,Suspension polymerization ,Protein Renaturation - Abstract
The poly(N-isopropylacrylamide) (PNIPA) hydrogel, which is a kind of temperature-sensitive polymer, was synthesized by inverse suspension polymerization. The microscopy and scan electron microscopy (SEM) of PNIPA hydrogel were studied. The microscope photograph showed that the particles were in the range of 0.2–0.5mm in diameter, with numerous conjoint pores about 1–2μm spreading all over the surface of the beads. The swelling properties of PNIPA gel beads indicated that the lower critical solution temperature (LCST) of the gel was 33°C. The PNIPA prepared was applied to the renaturation of bovine prethrombin-2 (pThr-2) from inclusion bodies produced in E. coli. It was observed that PNIPA was quite efficient in assisting protein renaturation at high protein concentration. When mixing with 105mg/mL PNIPA hydrogel during the refolding, the total activity of the thrombin was about 6222U/mL, compared with only 2800U/mL by simple dilution refolding. The kinetics of pThr-2 refolding with the absence or the presence of PNIPA was also studied respectively. The time required for the refolding with PNIPA gel was a little bit longer than that by the dilution method owing to the diffusion resistance of the protein into the network of the gel and the hydrophobic interaction between the protein and the polymer. The mechanism of the enhancement for the PNIPA gel to the refolding was further discussed. The porosity of the PNIPA hydrogel allows penetration of the unfolded protein into the inside of the polymer with a hydrophobic side chain, which can facilitate the formation of intermediate via hydrophobic interaction with the unfolded protein and the folding intermediate that are liable to re-aggregation. About 1.2mg of purified active thrombin could be recovered from 1 L of cells, which greatly facilitated the scale-up to the quantities of protein necessary for further functional and structural studies. A novel protein renaturation method mediated by PNIPA hydrogel beads, which highly increases the refolding efficiency with easy handling, recycling, and low cost, was proposed. © 2005 Wiley Periodicals, Inc. J Appl Polym Sci 96: 1734–1740, 2005
- Published
- 2005
43. Transgenic mice that express Cre recombinasein hypertrophic chondrocytes
- Author
-
Xiang Gao, Jishuai Zhang, Ning Hou, Nan Jiang, Xuan Cheng, Guan Yang, Youliang Wang, Xiao Yang, and Fang Cui
- Subjects
Male ,Genetically modified mouse ,Transgene ,Cell Culture Techniques ,lac operon ,Cre recombinase ,Mice, Transgenic ,In situ hybridization ,Biology ,Mice ,Chondrocytes ,Endocrinology ,Genes, Reporter ,Genetics ,medicine ,Animals ,Allele ,In Situ Hybridization ,Integrases ,Cartilage ,Gene targeting ,Cell Differentiation ,Cell Biology ,Molecular biology ,medicine.anatomical_structure ,Female ,Collagen Type X - Abstract
In order to investigate the physiological control of hypertrophic chondrocytes which present the terminally differentiated form of chondrocytes, we generated a mouse line expressing the Cre recombinase under the control of the mouse type X collagen (Col10a1) promoter. In situ hybridization analysis demonstrated the expression of Col10a1-Cre transgene in hypertrophic chondrocytes of femur at postnatal day 2 (P2). In order to test the excision activity of the Cre recombinase, the Col10a1-Cre transgenic line was crossed with the mouse strain carrying the Smad4 conditional alleles (Smad4co/co) and the reporter line ROSA26. Multiple tissue PCR of Col10a1-Cre;Smad4co/+ mice revealed the restricted Cre activity in tissues containing hypertrophic chondrocytes. LacZ staining revealed that the Cre activity was observed in the cartilage primordia of ribs at E14.5 and only detected in the lower hypertrophic region of ribs at P1. These data suggest that the Col10a1-Cre mouse line described here could be used to achieve conditional gene targeting in hypertrophic chondrocytes. genesis 42:33–36, 2005. © 2005 Wiley-Liss, Inc.
- Published
- 2005
44. ChemInform Abstract: Bis-Cyclometallated Gold(III) Complexes as Efficient Catalysts for Synthesis of Propargylamines and Alkylated Indoles
- Author
-
Man-Kin Wong, Jian Fang Cui, Hok Ming Ko, and Karen Ka Yan Kung
- Subjects
Silver salts ,Gold iii ,Chemistry ,Polymer chemistry ,General Medicine ,Alkylation ,Pyrrole derivatives ,Catalysis - Abstract
Stable bis-cyclometallated gold(III) complexes were developed as efficient catalysts for organic transformation reactions by using two strategies: (1) construction of distorted square planar gold(III) complexes and (2) dual catalysis by gold(III) complexes and silver salts.
- Published
- 2014
45. Alkaline cleavage of gypenosides and characterization of dammarane-type aglycones by gas chromatography-mass spectrometry
- Author
-
Peter Eneroth, Jan G. Bruhn, Jian-Fang Cui, Kazuko Yoshikawa, and Shigenobu Arihara
- Subjects
Chromatography ,Chemistry ,Dammarane ,Cleavage (crystal) ,Plant Science ,General Medicine ,Mass spectrometry ,Biochemistry ,Analytical Chemistry ,chemistry.chemical_compound ,Complementary and alternative medicine ,Drug Discovery ,Molecular Medicine ,Gas chromatography–mass spectrometry ,Food Science - Abstract
Five dammarane-type aglycones, released from gypenosides following an alkaline cleavage procedure previously developed for ginsenosides, were separated and characterized by gas chromatography-mass spectrometry (GC-MS) after trimethylsilylation. A satisfactory identification among isomers of 20(S)-protopanaxadiol or 20(S)-protopanaxatriol was obtained.
- Published
- 1998
46. ChemInform Abstract: A Concise Synthetic Approach Towards Hydroxytetraphenylenes
- Author
-
Jian Fang Cui, Henry N. C. Wong, and Hui Huang
- Subjects
Chemistry ,General Medicine ,Combinatorial chemistry - Abstract
Recent results in improving the syntheses of five symmetrical and unsymmetrical hydroxytetraphenylenes are reported.
- Published
- 2011
47. The prognosis of breast cancer in males a report of 335 cases
- Author
-
Rick Shallenberger, Rudolf Richter, Stanley Dische, Roelof Zewuster, Håkan Olsson, Zoltan Peter, J. W. van den Blink, Michel Durand, Mei Fang Cui, Franz J. Cleton, Vincent F. Guinee, Torgil Möller, and Warren W. Lane
- Subjects
Oncology ,Cancer Research ,medicine.medical_specialty ,Multivariate analysis ,business.industry ,Mammary gland ,Cancer ,Histology ,medicine.disease ,Surgery ,Breast cancer ,medicine.anatomical_structure ,Internal medicine ,medicine ,Axillary nodes ,Male Breast Carcinoma ,business ,Survival rate - Abstract
Background. Because of the relative rarity of breast cancer in males, data have not been sufficient to support a definitive analysis of pertinent prognostic factors. Remarkably, no studies of male patients with breast cancer have presented survival information based on the number of histologically positive axillary nodes, the most sensitive single indicator of prognosis in women with breast cancer. Methods. In this study, the clinical course of breast cancer was documented for 335 male patients registered from 1965 through 1986. For patients to be eligible, diagnoses had to be made within 3 months of registration and the patients had to have histologic confirmation and receive part or all of their initial treatment at 1 of 11 cancer centers participating in the International Patient Data Exchange System. Results. The survival rate at 10 years was 84% for patients with histologically negative nodes, 44% for those with one to three positive nodes, and 14% for the group with four or more histologically positive nodes. The survival rates at 5 years were 90%, 73%, and 55%, respectively. In a multivariable analysis, the risk of death due to breast cancer for a patient with four or more histologically positive nodes was 6.75 times that of a patient with negative nodes. Conclusions. The findings of the authors indicate the following: (1) The number of histologically positive axillary nodes and, to a lesser degree, tumor diameter are significant prognostic factors for breast cancer in male patients. (2) The prognosis of breast cancer is the same in male and female patients when compared on the basis of the number of histologically positive nodes. Cancer 1993; 71:154‐61. (Less)
- Published
- 1993
48. Analysis of alkaloids in ChineseEphedra species by gas chromatographic methods
- Author
-
Jian-Sheng Zhang, Jian-Fang Cui, Tong-Hui Zhou, and Zhi-Cen Lou
- Subjects
Nitrogen–phosphorus detector ,Chromatography ,Chemistry ,Methylephedrine ,Plant Science ,General Medicine ,Mass spectrometry ,Pseudoephedrine ,Biochemistry ,Analytical Chemistry ,Complementary and alternative medicine ,Drug Discovery ,medicine ,Molecular Medicine ,Gas chromatography ,Ephedrine ,Gas chromatography–mass spectrometry ,Food Science ,medicine.drug - Abstract
The six Ephedra alkaloids (ephedrine, norephedrine, pseudoephedrine, norpseudoephedrine, methylephedrine and methylpseudoephedrine) in 12 species of Chinese Ephedra collected in 16 districts of China were separated and identified by gas chromatography combined with mass spectrometry following trimethylsilylation of the extract. The alkaloidal concentrations were determined using a gas chromatograph equipped with a nitrogen phosphorus detector. The methods described are sensitive, accurate and simple to perform.
- Published
- 1991
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.