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1. Differential regulation of mycelial growth and aflatoxin biosynthesis by Aspergillus flavus under different temperatures as revealed by strand‐specific RNA‐Seq

Author

Guomin Han, Kai Zhao, Xiaodan Yan, Fangzhi Xiang, Xuede Li, and Fang Tao

Subjects
aflatoxin biosynthesis, Aspergillus flavus NRRL 3357, pathways, regulation, strand‐specific RNA‐Seq, temperature, Microbiology, QR1-502
Abstract

Abstract Although several regulatory pathways have been reported for Aspergillus flavus, the regulation of aflatoxin production and mycelial growth under different temperatures remains unclear. In this study, A. flavus differentially expressed genes (DEGs) and regulatory pathways were analyzed under three temperatures, by strand‐specific RNA‐Seq. Results show that a total of 2,428 and 1,474 DEGs were identified in fungal mycelia cultured at 20°C and 37°C, respectively, as compared with the control (28°C). Approximately ~ 79% of DEGs in the 37°C samples were up‐regulated genes, while ~ 63% of DEGs in the 20°C samples were down‐regulated genes. Most of the DEG pathways enriched by lower temperatures differed from those enriched by higher temperatures, while only a small portion of the pathways were shared by A. flavus grown under different temperatures. Aflatoxin biosynthesis, Butanoate metabolism, oxidation–reduction process, and benzene‐containing compound metabolic process were the shared down‐regulated pathways, while steroid biosynthesis, oxidoreductase activity, cellular protein modification process, DNA binding, protein complex were the shared up‐regulated pathways between lower and higher temperatures. The shared genes and pathways are the key regulatory candidates for aflatoxin biosynthesis with changes of temperature. In addition, the identification of both up‐regulated and down‐regulated genes provides a useful gene set for further investigation of the aflatoxin biosynthesis among Aspergillus.

Published
2019
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7. Dasatinib‐induced pulmonary arterial hypertension in pediatric acute lymphoblastic leukemia with Philadelphia chromosome: A report of two cases

Author

Sujie Tang, hao xiong, Zhi Chen, Li Yang, Ming Sun, Wenjie Lu, Zhuo Wang, Fang Tao, Min Wu, Linlin Luo, and Zuofeng Li

Subjects
Oncology, Pediatrics, Perinatology and Child Health, Hematology
Abstract

Background: Long-term oral dasatinib administration can induce pulmonary arterial hypertension (PAH) in pediatric patients with Philadelphia chromosome-positive (Ph ) acute lymphoblastic leukemia (ALL). We describe the findings in two pediatric cases involving Ph ALL patients who developed cardiovascular events such as PAH after dasatinib treatment, and present a review of the related literature. Case presentation: Two pediatric patients diagnosed with B-cell ALL ( BCR-ABL P190 fusion gene positive) received conventional chemotherapy and imatinib simultaneously, which was then changed to dasatinib because of a partial response. The two patients developed PAH after 18 months and 6 years of dasatinib therapy. All signs and symptoms improved after immediate discontinuation of dasatinib and symptomatic treatment. Conclusions: Pediatric Ph ALL patients receiving dasatinib should be carefully monitored for serious cardiopulmonary and vascular events such as PAH. Development of adverse reactions should be followed by immediate and permanent discontinuation of oral dasatinib. Dynamic monitoring by echocardiography is recommended when administering dasatinib for maintenance therapy.

Published
2023

9. Identification of the prognostic value of elevated ANGPTL4 expression in gallbladder cancer‐associated fibroblasts

Author

Wei Sun, Mu-Su Pan, Yue-Zu Fan, Xin-Ping Li, Fang-Tao Wang, and Mohamed Hassan

Subjects
Male, Cancer Research, cancer‐associated fibroblast, Metastasis, gallbladder cancer, Mice, ANGPTL4, Nude mouse, Cancer-Associated Fibroblasts, Stroma, In vivo, medicine, Angiopoietin-Like Protein 4, Animals, Humans, Radiology, Nuclear Medicine and imaging, Gallbladder cancer, RC254-282, Research Articles, Aged, Cancer Biology, biology, business.industry, Neoplasms. Tumors. Oncology. Including cancer and carcinogens, Middle Aged, Prognosis, medicine.disease, biology.organism_classification, Survival Analysis, Oncology, Tumor progression, Cancer research, Immunohistochemistry, Female, Gallbladder Neoplasms, business, Research Article
Abstract

Background Cancer‐associated fibroblasts (CAFs) with different gene profiles from normal fibroblasts (NFs) have been implicated in tumor progression. Angiopoietin‐like protein 4 (ANGPTL4) has been shown to regulate tumor angiogenesis and metastasis, and predict poor prognosis. However, the ANGPTL4 expression in CAFs, especially in gallbladder CAFs (GCAFs) and its relationship with patient prognosis is unclear. Methods Affymetrix gene profile chip analysis in vitro was performed to detect the different gene expression profiles between GCAFs and NFs. RT‐qPCR, immunohistochemistry, and western blotting were performed to investigate the different expression levels of ANGPTL4 in GCAFs/NFs in vitro and in an in vivo nude mouse model of xenograft tumors. Finally, the ANGPTL4 expression was investigated in the stroma of different lesion tissues of the human gallbladder by immunohistochemistry, especially the expression in GCAFs in vivo by co‐immunofluorescence, and their prognostic significance in patients with gallbladder cancer (GBC) was assessed. Results ANGPTL4 was upregulated in both GCAFs in vitro and in the xenograft stroma of nude mice in vivo, and its expression was also significantly upregulated in human GBC stroma co‐localized with the interstitial markers fibroblast secreted protein‐1 and α‐smooth muscle actin. In addition, the elevated ANGPTL4 expression in GCAFs was correlated with tumor differentiation, liver metastasis, venous invasion and Nevin staging, and GBC patients with an elevated ANGPTL4 expression in GACFs were found to have a lower survival rate. Conclusions Increased ANGPTL4 expression in GCAFs correlates with poor patient prognosis, which indicates a potential therapeutic target for human GBCs., ANGPTL4 expression was upregulated in GCAF, which was significantly related to the degree of clinical GBC differentiation, liver metastasis, venous invasion, and Nevin staging, and GBC patients with elevated ANGPTL4 expression in GCAF had lower survival rate.

Published
2021

12. Differential effects of alcohol‐drinking patterns on the structure and function of the brain and cognitive performance in young adult drinkers: A pilot study

Author

Guo, Xiaobing, primary, Yan, Tongjun, additional, Chen, Min, additional, Ma, Xiaoyan, additional, Li, Ranli, additional, Li, Bo, additional, Yang, Anqu, additional, Chen, Yuhui, additional, Fang, Tao, additional, Yu, Haiping, additional, Tian, Hongjun, additional, Chen, Guangdong, additional, and Zhuo, Chuanjun, additional

Published
2021
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26. Sex pheromone titre in the glands ofSpodoptera liturafemales: circadian rhythm and the effects of age and mating

Author

Ling-Yan Huang, Qin Lu, Peng Chen, Jin Xu, Hui Ye, Xia-Fei Wang, Jian-Yu Deng, and Fang-Tao Liu

Subjects
0106 biological sciences, medicine.medical_specialty, biology, Physiology, media_common.quotation_subject, Spodoptera litura, Insect, biology.organism_classification, 010603 evolutionary biology, 01 natural sciences, 010602 entomology, Male accessory gland, Endocrinology, Insect Science, Internal medicine, Sex pheromone, medicine, Pheromone, Circadian rhythm, Reproduction, Mating, Ecology, Evolution, Behavior and Systematics, media_common
Abstract

The present study investigates the effects of age and mating status on the circadian variations of gland sex pheromone titre in female Spodoptera litura Fabricius. Similar to other nocturnal moths, S. litura females exhibit circadian variations of gland sex pheromone contents, with higher levels during scotophase and lower levels during photophase. The sex pheromone titre in the glands peaks during the first scotophase after eclosion and sharply declines afterwards. Higher pheromone contents during scotophase may facilitate female reproductive activities, and the negative relationship between pheromone titre and female calling is likely the result of pheromone release during female calling. Interestingly, the present study demonstrates that mated S. litura females have significantly higher sex pheromone titre in their pheromone glands (PGs) than virgin females. This finding contrasts with all previous studies of other insect species, in which mating generally reduces the sex pheromone titre in female PGs. In S. litura, mating and male accessory gland fluids can suppress female calling behaviours and re-matings. These results suggest that the suppression of female calling behaviours by mating and male accessory gland fluids may significantly reduce the release of sex pheromones and thus result in higher sex pheromone titre in the PGs of mated females.

Published
2017

34. Evaluation of the damage by dyestuffs and NaCl on Phanerochaete chrysosporium

Author

Minsheng Huang, Linhui Wu, Fang Tao, Shang Gao, and Cheng Chen

Subjects
Persistent organic pollutant, Environmental Engineering, biology, Renewable Energy, Sustainability and the Environment, Chemistry, General Chemical Engineering, biology.organism_classification, Microbiology, Wastewater, Oxidizing agent, Environmental Chemistry, Phanerochaete, White rot fungus, Waste Management and Disposal, General Environmental Science, Water Science and Technology, Chrysosporium, Nuclear chemistry
Abstract

White rot fungus, Phanerochaete chrysosporium (P. chrysosporium), is oxidizing persistent organic pollutants (POPs) including dyes. The approaches of amplified fragment length polymorphism (AFLP) analysis and transmission electron microscope (TEM) observation were carried out to evaluate DNA and cell damaged by dyestuff and NaCl of different concentrations on P. chrysosporium. According to the data of P. chrysosporium cultivation and TEM observation, the damage of NaCl on P. chrysosporium was more significant than dyestuff. In addition, UPGMA method was performed to analyze the damage on P. chrysosporium by different concentration of NaCl and dyestuff by MVSP (Version 3.1). It showed that the DNA similarity between No. 2 (100 mg/L dyestuff and 15% NaCl) and No. 3 (100 mg/L dyestuff and 8% NaCl) was 66.7% when the concentrations of dyestuff was 100 mg/L. The DNA similarity between No. 3 (100 mg/L dyestuff and 8% NaCl) and No. 4 (300 mg/L dyestuff and 8% NaCl) was 88.9% when NaCl was kept at 8%, which was higher than 66.7%. No. 5 (300 mg/L dyestuff and 15% NaCl) was the most serious group. These results showed the damage by NaCl was significantly serious than that of dyestuffs. Therefore, the concentrations of NaCl in real dyestuff wastewater should be kept within 8%, keeping the treatment efficiency. © 2010 American Institute of Chemical Engineers Environ Prog, 2010

Published
2010

41. Suppression of 6A8 ?-mannosidase gene expression reduced the potentiality of growth and metastasis of human nasopharyngeal carcinoma

Author

Ling Ping Zhu, Fang tao Zhao, Yin Liu, Wei Yue, Yang Gao, Geng-Xian Shi, and Yu Lan Jin

Subjects
Cancer Research, Pathology, medicine.medical_specialty, DNA, Complementary, Cell, Mice, Nude, Biology, Transfection, DNA, Antisense, Gene Expression Regulation, Enzymologic, Colony-Forming Units Assay, Mice, alpha-Mannosidase, Cell Adhesion, Tumor Cells, Cultured, Carcinoma, medicine, Animals, Humans, MTT assay, Pseudopodia, Reverse Transcriptase Polymerase Chain Reaction, Cell growth, Cadherin, Cell adhesion molecule, Nasopharyngeal Neoplasms, Cadherins, medicine.disease, Molecular biology, In vitro, medicine.anatomical_structure, Oncology, Nasopharyngeal carcinoma, Cell Division
Abstract

Suppression of α-mannosidases by chemicals has been shown to reduce the potentiality of growth and metastasis of various tumors. In our study, the effect of 6A8 α-mannosidase (MAN 6A8), recently discovered in our laboratory, on malignant behaviors of tumor cells was examined. Since the suppressive effect of chemicals on α-mannosidase is not specific, antisense technique was used to specifically inhibit expression of the MAN 6A8 in human nasopharyngeal carcinoma cells, CNE-2L2. Two cell clones, AS1 and AS2, with pronounced suppression of MAN 6A8 expression were developed. Wild-type (W), mock-transduced (M) and irrelevant DNA-transduced (IR) CNE-2L2 cells with normal expression of the enzyme were used as controls. Malignant behaviors of the cells were examined. Significant inhibition of growth of AS cells in vitro measured by MTT assay, colony formation and anchorage-independent colony formation was found. Pronounced inhibition of formation of tumors from AS cells inoculated into nude mice and metastasis was also observed. W, M and IR cells cultured in plate wells appeared dispersed with a fibroblastic or epithelial morphology, whereas AS cells were in compact sheets with an epithelioid organization. Since E-cadherin is the key factor in homophilic adhesion of epithelial cells, its expression on the surface of CNE-2L2 cells was determined. E-cadherin expression on AS cells was enhanced, whereas it was markedly diminished on W, M and IR cells. In addition, lamellipodia, which play an important role in cell spreading and mobility, almost disappeared on AS cells. The results demonstrate a significant suppressive effect of reduced expression of MAN 6A8 on malignant behaviors of CNE-2L2 cells. © 2003 Wiley-Liss, Inc.

Published
2003

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