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Previous research on calling has resulted in a model that relates living a calling to life satisfaction through job satisfaction and life meaning. However, no research has examined this model in a lesbian, gay, bisexual (LGB) population. With a sample of 171 sexual minority adults, the current study aimed to (a) test the fit of an established model of living a calling and life satisfaction and (b) examine how having a supportive LGB workplace climate functioned within this model. Results revealed an excellent fit of the model and that a supportive LGB workplace climate predicted both living a calling and job satisfaction. In addition, the relation of living a calling to life satisfaction was fully mediated by life meaning and job satisfaction, and the relation of climate to life satisfaction was mediated by job satisfaction. These results suggest an expansion of the potential utility of the calling construct to an LGB population.