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Your search keyword '"Elkins, W. R."' showing total 2 results
Start Over Author "Elkins, W. R." Publisher wiley
2 results on '"Elkins, W. R."'

1. Immunization with whole inactivated vaccine protects from infection by SIV grown in human but not macaque cells.

Author

Goldstein S, Elkins WR, London WT, Hahn A, Goeken R, Martin JE, and Hirsch VM

Subjects
Animals, Cell Line, Enzyme-Linked Immunosorbent Assay, Humans, Macaca mulatta, Simian Acquired Immunodeficiency Syndrome prevention & control, Simian Immunodeficiency Virus growth & development, Time Factors, Viremia immunology, Viremia prevention & control, Antibodies, Viral blood, SAIDS Vaccines immunology, Simian Acquired Immunodeficiency Syndrome immunology, Simian Immunodeficiency Virus immunology
Abstract

Homologous SIVsm stocks were generated by passage of a macaque isolate of SIVsm (SIVsm/E660) in human CEM x 174 cells or macaque peripheral blood mononuclear cells. Macaques were immunized with whole inactivated SIV vaccine consisting of virus generated by transfection of CEM x 174 cells with the SIVsmH4 clone and were challenged with either cell-free stock. Only vaccinees challenged with virus generated in human cells were protected from infection. This confirms the species-specificity of whole inactivated vaccine-mediated protection.

Published
1994
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2. Adaptation of HIV-1 to pigtailed macaques.

Author

Gartner S, Liu Y, Polonis V, Lewis MG, Elkins WR, Hunter EA, Miao J, Corts KJ, and Eddy GA

Subjects
Acclimatization, Animals, Base Sequence, Cells, Cultured, DNA Primers, DNA, Viral analysis, DNA, Viral biosynthesis, HIV Antibodies analysis, HIV Antibodies biosynthesis, HIV-1 genetics, HIV-1 pathogenicity, Humans, Macaca fascicularis, Macaca mulatta, Macaca nemestrina, Molecular Sequence Data, Polymerase Chain Reaction, Species Specificity, T-Lymphocytes immunology, Viral Proteins analysis, Viral Proteins biosynthesis, Genes, gag, HIV-1 physiology, T-Lymphocytes virology
Abstract

In vitro infectivity experiments were performed to assess the susceptibility of cells from various monkey species to HIV-1. T lymphocytes from pigtailed macaques, but not those from rhesus or cynomolgus monkeys, were susceptible to infection, but virus expression was limited. The majority of HIV-1 isolates were unable to productively infect pigtailed macaque cells. Inoculation of autologous, HIV-1 expressing cells led to establishment of persistent infection in pigtailed macaques as evidenced by recovery of infectious virus and development of virus-specific antibody responses.

Published
1994
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