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Soil properties and stable carbon isotope ratios contained in the soil organic matter (SOM) were used to investigate the change in vegetative history of land cleared anciently for maize (Zea mays L.) agriculture in the Petexbatun region of Guatemala. Maize and other C4 plants associated with land clearance leave a carbon isotopic signature in the SOM different from the C3 plants of native forest vegetation. Soil profiles were collected from various landscape features around the Classic Maya site of Aguateca: control locations (areas likely not used in ancient agriculture), defensible locations (areas near defensive walls), rejolladas (natural karst depressions), upland locations (well-drained soils atop the Aguateca escarpment), and bajos (seasonal and perennial wetlands). The chemical and physical properties of the profiles were examined and the soils were taxonomically classified to the great group level. The changes in d13C with soil depth were determined and compared statistically. The 13C enrichment of the SOM in bajo and rejollada profiles were similar and were significantly (p < 0.05) greater than the control, defensible, and upland soils. This isotopic signature of sustained C4 vegetation was likely associated with forest clearance and ancient Maya agriculture. Both the bajo and rejollada landscape features appear to have been valuable agricultural resources for ancient Maya. © 2009 Wiley Periodicals, Inc.

The ancient Maya subsisted in an environment limited by shallow soils and unpredictable weather patterns until their collapse ∼A.D. 800–900. Ancient subsistence can be a difficult subject, with little physical evidence of agricultural artifacts and structures. This study characterized soil profiles and utilized changes in stable carbon isotope ratios of soil organic matter (SOM) to locate and interpret areas of ancient C4 plant growth and maize (Zea mays) cultivation among the Maya. The investigation indicated some of the challenges the Maya faced, including shallow and sloped soils in some areas. The C4 plant signature was found in seasonal wetland soils on the opposite side of the Laguneta Aguateca from the ruins of Aguateca, but not in the perennial wetlands on the immediate side. No C4 plant signature was detected in the shoulder and backslope soils. Based on these findings, the ancient Maya of Aguateca probably adapted to their environment by farming rich toeslope soils. It is possible that maize was also grown in the seasonal wetlands adjacent to the site. If the steep backslope soils around Aguateca were used in ancient agriculture, the evidence has probably eroded away. © 2007 Wiley Periodicals, Inc.