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A monoclonal antibody (NIM-M7) has been prepared which reacts with a major surface antigen of adult males and females of Trichinella spiralis. This specificity is only demonstrable when the antigen is liberated by detergent solubilization of surface-labelled worms. When reacted with living adults, on the other hand, NIM-M7 reacts well with only the eversible cloaca, or copulatory bell, of the male, binding weakly, if at all, to other surface areas of male or female worms. A similar staining pattern is also given by Concanavalin A. The differential staining given by NIM-M7 must indicate a molecular difference between the organization of the same surface antigen on the cuticular surface of the copulatory bell and other areas of the worm surface. This example of regional specialization demonstrates that the nematode cuticle is not necessarily chemically uniform.