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To date, only a few clinical and neurophysiological studies have assessed the features of valproate-induced tremor (VIT), and whether valproate (VPA) affects voluntary movements is underinvestigated.To better characterize the clinical and neurophysiological features of VIT in patients with epilepsy and the effect of VPA on the execution of voluntary movement.We tested 29 patients with VIT (13 taking VPA alone and 16 taking VPA plus other antiepileptics). Patients underwent a neurological examination, video recordings and kinematic assessments of postural, kinetic, and resting upper limb tremor using a motion analysis system. Movement execution was tested by kinematic assessment of finger tapping. Data of patients with VIT were compared with those of 13 patients with epilepsy taking VPA but without tremor, 13 patients with epilepsy who were not on VPA treatment, 20 patients with Parkinson's disease (PD), and 20 healthy controls (HCs).Clinical and kinematic evaluations showed that tremor in patients taking VPA alone was less severe than tremor in patients taking VPA plus other antiepileptics. All patients taking VPA, regardless of the presence of tremor, performed slower finger tapping compared with HCs, similar to what was observed in PD, although with no sequence effect. Patients with epilepsy without VPA showed a normal motor performance.Tremor and movement slowness are motor signs induced by VPA. VIT severity is exacerbated when VPA is taken in combination with other antiepileptics. VPA-induced slowness occurs regardless of tremor, may precede tremor development, and is not attributed to epilepsy.

Parvovirus B19 (B19V) infection in pregnancy is mostly asymptomatic, but can cause complications including abortion and fetal hydrops. Although its infection is ubiquitous, seroprevalence among pregnant women varies according to different geographical areas. Since seroprevalence data in Italy are limited, the prevalence of antibodies and DNA in pregnant women was evaluated retrospectively, correlating the clinical situation of mothers and newborns. One thousand eight hundred and ninety-three sequential sera were examined from pregnant women (60.8% in the first trimester, 16.6% in the second one, and 22.6% in the third one, respectively) for anti-B19V IgG and IgM (confirmed by immunoblot); 1402 (74.1%) were of Italian origin and 491 (25.9%) non-Italian women. Molecular tests were used to search for viral genome. One thousand three hundred and fifteen (69.5%) samples were IgG-positive, 21 (1.1%) IgM-positive, and 578 (30.5%) nonimmune. The difference in IgG seroprevalence between Italian (71.1%) and non-Italian women (64.8%) was statistically significant. Of the 21 IgM-positive women, 16 were confirmed positive also by immunoblot (prevalence: 0.8%), of which 11 were viraemic (prevalence: 0.6%; mean 1.3 × 10

Objective To assess prognostic patterns and investigate clinical and electroencephalography (EEG) variables associated with persistent treatment resistance in a population of genetic generalized epilepsy (GGE) patients with a long-term follow-up. Methods Data from GGE patients followed from 1975 to 2019 were reviewed retrospectively. Subjects with a follow-up >10 years, starting from epilepsy diagnosis, were included. Persistent treatment resistance was defined as the absence of any period of remission ≥1 year despite treatment with two appropriate and adequate antiepileptic drugs (AEDs). Results One hundred ninety-nine patients were included. The median age was 39.5 years (interquartile range [IQR] 30-49) and the median follow-up was 27 years (IQR 18-35). The most common syndrome was juvenile myoclonic epilepsy (JME), diagnosed in 44.2% of patients. During follow-up, 163 subjects (81.9%) experienced 3-year remission from any seizure type, whereas 5- and 10-year remission occurred in 141 (70.8%) and 92 (46.2%) cases, respectively. The most common prognostic pattern was a relapsing-remitting course, observed in 80 patients (40.2%), whereas 29 (14.6%) displayed persistent treatment resistance. According to multivariable logistic regression analysis, febrile seizures (FS), specific EEG patterns (namely generalized paroxysmal fast activity, GPFA) and valproate (VPA) resistance were the only variables significantly associated with persistent treatment resistance. JME was the only epilepsy syndrome statistically associated with persistent treatment resistance in univariable logistic regression analysis. Significance Persistent treatment resistance was observed in almost 15% of GGE patients followed in a tertiary epilepsy center. A worse outcome was associated with specific clinical variables (JME, FS) and EEG patterns (GPFA).

A growing body of evidence suggests that autophagy inhibition enhances the effectiveness of chemotherapy, especially in difficult-to-treat cancers. Existing autophagy inhibitors are primarily lysosomotropic agents. More specific autophagy inhibitors have not yet been developed. The microtubule-associated protein 1A/1B light chain 3B protein, LC3B, is an adapter protein that mediates key protein-protein interactions at several points in autophagy pathways. In this work, we use a known peptide ligand as a starting point to develop improved LC3B inhibitors. We obtained structure-activity relationships that quantify the binding contributions of peptide termini, individual charged residues, and hydrophobic interactions. Based on these data, we used artificial amino acids and diversity-oriented stapling to improve affinity and resistance to biological degradation while maintaining or improving LC3B affinity and selectivity. These peptides represent the highest-affinity LC3B-selective ligands reported to date, and they will be useful tools for further elucidation of LC3B’s role in autophagy and in cancer.

Socioeconomic disadvantage is extremely common among women with depressive symptoms presenting for women's health care. While social stressors related to socioeconomic disadvantage can contribute to depression, health care tends to focus on patients' symptoms in isolation of context. Health care providers may be more effective by addressing issues related to socioeconomic disadvantage. It is imperative to identify common challenges related to socioeconomic disadvantage, as well as sources of resilience. In this qualitative study, we interviewed 20 women's health patients experiencing depressive symptoms and socioeconomic disadvantage about their views of their mental health, the impact of social stressors, and their resources and skills. A Consensual Qualitative Research approach was used to identify domains consisting of challenges and resiliencies. We applied the socioecological model when coding the data and identified cross-cutting themes of chaos and distress, as well as resilience. These findings suggest the importance of incorporating context in the health care of women with depression and socioeconomic disadvantage.

Does regional autonomy lead to better local public services? We investigate this issue using measures of public service performance and autonomy at the region level in 171 European regions. We introduce a novel dose-response approach which identifies the pattern of the effect of regional autonomy on the performance of public services. The relationship between the level of regional autonomy and the provision of local public services exhibits a u-shape: both low and high autonomy lead to better local public services. This speaks against the presence of one optimal level of autonomy and policy recommendations based on the view that more decentralisation is always desirable. It shows that different institutional settings can be economically viable and efficient.

We model and solve the Rainbow Cycle Cover Problem (RCCP). Given a connected and undirected graph G = ( V , E , L ) and a coloring function l that assigns a color to each edge of G from the finite color set L, a cycle whose edges have all different colors is called a rainbow cycle. The RCCP consists of finding the minimum number of disjoint rainbow cycles covering G. The RCCP on general graphs is known to be NP-complete. We model the RCCP as an integer linear program, we derive valid inequalities and we solve it by branch-and-cut. Computational results are reported on randomly generated instances. © 2016 Wiley Periodicals, Inc. NETWORKS, 2016

The aim of this study was to compare the data obtained using the new LIAISON® XL chemiluminescence system to search for HBsAg, anti-HCV, and anti-HIV1-2/p24 Ag with those obtained using the VITROS system currently adopted by the Microbiology Unit of the Hospital of Legnano. Routine samples of patients who were referred by practitioners for the determination of HBsAg (1,000 samples) and/or anti-HCV (1,002 samples) and/or anti-HIV1-2 (995 samples) were simultaneously analyzed using both systems. The concordant positive and discordant samples were re-examined for confirmation by means of an HBsAg neutralization assay, anti-HCV immunoblot, or anti-HIV1-2 Western blot; HBV-DNA, or HCV-RNA or HIV-RNA was also sought in the discordant samples. Samples of patients known to be positive were tested (100 HBsAg positive, 100 anti-HCV positive, and 100 HIV 1-2 positive) as well throughout treatment, with viremia levels becoming undetectable after treatment. The HBsAg, anti-HCV, and anti-HIV1-2 concordance between the two systems in routine series was respectively 99.8%, 98.5% and 99.7%, and 100% for all markers in samples known positive. The various molecular biology and confirmatory tests of the discordant samples were all negative (except for one anti-HCV positive sample). Measure of Cohen's kappa coefficient for HBsAg, anti-HCV, and anti-HIV gave K values of respectively 0.992, 0.946, and 0.980. In conclusion, the performance of the LIAISON® XL system in the routine laboratory determination for all three markers was comparable with that of the VITROS system. J. Med. Virol. 89:489-496, 2017. © 2016 Wiley Periodicals, Inc.

Recent community-based research indicates that the prevalence of intimate partner violence (IPV) in the Deaf community exceeds known rates among hearing individuals, yet little is known about services available to Deaf IPV victims. Given the inaccessibility of IPV services, providers (doctors, psychologists, and lawyers) who know American Sign Language become IPV providers while addressing myriad comorbid issues that affect Deaf clients. This article presents data drawn from transcripts of semistructured interviews with 12 interdisciplinary providers who serve the Deaf population. We sought to understand the etiology of abuse involving Deaf victims and what, if any, services are available. We explore similarities and differences between service provision for hearing and Deaf IPV victims. FINDINGS suggest providers working with Deaf IPV victims typically (a) work with victims and perpetrators alike, (b) provide services to couples and families, (c) serve larger geographical areas, and (d) address stigmatization. Perhaps the most important finding is that standard terminology in IPV measures, without linguistic and cultural modifications, may be invalid for use with Deaf individuals. Policy implications are discussed. Language: en

Given an undirected and vertex weighted graph G=V,E,w, the Weighted Feedback Vertex Set Problem consists of finding the subset Fi¾?V of vertices, with minimum weight, whose removal results in an acyclic graph. Finding the minimum feedback vertex set in a graph is an important combinatorial problem that has a variety of real applications. In this article, we introduce a memetic algorithm for this problem. We propose an efficient greedy procedure that quickly generates chromosomes with specific characteristics and a wise application of recent local search procedures based on k-diamonds. Computational results show that the proposed algorithm outperforms the effectiveness of two other metaheuristics recently proposed in the literature for this problem. © 2014 Wiley Periodicals, Inc. NETWORKS, Vol. 644, 339-356 2014

Pregnant women are not screened for HBsAg and anti-HCV antibodies in many African countries. As there are few data concerning the prevalence of HBV, HDV, and HCV serological markers in Benin, the aim of this study was to evaluate their 2011 prevalence in pregnant women undergoing HIV screening in a rural area of north Benin, and compare the data with those reported for the same area in 1986. The sera of 283 women were examined for HBsAg, anti-HBs, anti-HBc, anti-HCV, and anti-HIV 1/2 antibodies. In the case of HBsAg positivity, a search was made for the HBeAg, anti-HDV, and HBV genotypes; in the case of anti-HCV positivity, a search was made for the HCV genotypes. HBsAg, anti-HBs, anti-HBc, anti-HCV, and anti-HIV 1/2 were positive in respectively 44 (15.5%), 82 (29.0%), 234 (82.7%), 21 (7.4%), and nine samples (3.2%). Of the HBsAg-positive samples, five (11.4%) were positive for HBeAg, five (11.4%) for anti-HDV, and 19 for HBV genotype E. Of the anti-HCV-positive samples, five were positive for genotype 2a/2c and one for genotype 1a. The prevalence of anti-HBc alone (HBsAg and anti-HBs negative) was very high (41.3%). In comparison with the 1986 data, the prevalence of HBsAg and anti-HBc remained unchanged, that of HBeAg and anti-HDV had decreased, and that of anti-HIV 1/2 had increased. As these data confirm that HBV and HCV are highly endemic in the study area, it may be appropriate to introduce HBsAg and anti-HCV screening for pregnant women. J. Med. Virol. 86:1281-1287, 2014. © 2014 Wiley Periodicals, Inc.

objectives Toxoplasma gondii, cytomegalovirus (HCMV) and rubella virus infections are amongthe most serious of those contracted during pregnancy in terms of foetal consequences. Toxoplasma,HCMV and rubella antibody screening is unusual in Africa, and there are few published data. Theaim of this study was to evaluate the prevalence of these markers among pregnant women innorthern Benin on the occasion of routine syphilis screening.methods Toxoplasma, HCMV and rubella IgG and IgM antibodies were determined in the serumof 283 women attending Saint Jean de Dieu de Tanguieta hospital, using an enzyme immunoassay,and IgM were confirmed using an enzyme-linked fluorescent assay (ELFA). In the case of IgMpositivity, the avidity of anti-HCMV and anti-Toxoplasma IgG was measured. Total anti-Treponemapallidum antibodies were determined using an enzyme immunoassay and confirmed byimmunoblotting. In the case of positivity, the Venereal Disease Research Laboratory (VDRL) test wasused.results The prevalence of anti-Toxoplasma, anti-HCMV, anti-rubella IgG and total anti-Treponemaantibodies was, respectively, 30.0%, 100%, 94% and 2.5%. The VDRL test was positive in 62.5%of the anti-Treponema-positive samples. The prevalence of anti-Toxoplasma, anti-HCMV andanti-rubella IgM was, respectively, 0.4%, 1.4% and 0%. There were no statistically significantdifferences in terms of age class or trimester of pregnancy. Anti-Toxoplasma and anti-HCMV IgGavidity was always high.conclusions The prevalence of HCMV and rubella antibodies is high in northern Benin, whereasthat of Toxoplasma antibodies is lower. As nearly two-thirds of the pregnant women were anti-Toxoplasma seronegative, antibody screening should be introduced.keywords Africa, HCMV, pregnancy, rubella, syphilis, ToxoplasmaIntroductionToxoplasma gondii, human cytomegalovirus (HCMV)and rubella virus are the major causes of congenital infec-tions. For toxoplasmosis, the screening tests in pregnancyhave been developed to prevent congenital infection, buttheir use in industrialised countries is related to the prev-alence of the infection, which varies widely in differentcountries. In sub-Saharan Africa, the prevalence rangesfrom 20% to 90% (Rodier et al. 1995; Adou-Bryn et al.2004; Simpore et al. 2006; Ndiaye et al. 2007

(Z)-3-Substituted-2-(4-pyridyl)-acrylonitriles, a reactive class of Michael acceptors obtained exclusively as a single (Z) isomer, reacted with un-substituted isocyanoacetate esters mediated by phase-transfer catalysis to give, after base promoted cyclisation, functionalized imines in up to 94% ee and as a single diastereoisomer.

To each tagged triangulation of a surface with marked points and non-empty boundary we associate a quiver with potential, in such a way that whenever we apply a flip to a tagged triangulation, the Jacobian algebra of the QP associated to the resulting tagged triangulation is isomorphic to the Jacobian algebra of the QP obtained by mutating the QP of the original one. Furthermore, we show that any two tagged triangulations are related by a sequence of flips compatible with QP-mutation. We also prove that for each of the QPs constructed, the ideal of the non-completed path algebra generated by the cyclic derivatives is admissible and the corresponding quotient is isomorphic to the Jacobian algebra. These results, which generalize some of the second author's previous work for ideal triangulations, are then applied to prove properties of cluster monomials, like linear independence, in the cluster algebra associated to the given surface by Fomin-Shapiro-Thurston (with an arbitrary system of coefficients)., Comment: v4: Added: Comparison of our results on linear independence of cluster monomials with results obtained by other authors; a couple of examples and figures; references. Some typos corrected. Final version, to appear in Compositio Mathematica. 27 pages, 9 figures

This article studies the double traveling salesman problem with two stacks. A number of requests have to be served where each request consists in the pickup and delivery of an item. All the pickup operations have to be performed before any delivery can take place. A single vehicle is available that starts from a depot, performs all the pickup operations and returns to the depot. Then, it performs all the delivery operations and returns to the depot. The items are loaded in two stacks, each served independently from the other with a last-in-first-out policy. The objective is the minimization of the total cost of the pickup and delivery tours. We propose a branch-and-bound approach to solve the problem. The algorithm uses properties of the problem both to tighten the lower bounds and to avoid the exploration of redundant subtrees. Computational results performed on benchmark instances reveal that the algorithm outperforms the other exact approaches for this problem. © 2012 Wiley Periodicals, Inc. NETWORKS, 2013 © 2013 Wiley Periodicals, Inc.

Every day, judges are faced with decisions regarding intimate partner violence (IPV) victims' requests for protection orders, custody arrangements, and visitation schedules. To make informed decisions, judges must understand victims' risk for future violence. This mixed method study explores the extent to which protection order petitions (n=169) communicate victims' current danger and future risk of violence. Methods included interviews coupled with an archival review of court petitions. Findings suggest judges are inadequately prepared to render decisions to improve victim safety in the absence of standardized risk assessments. The Danger Assessment provides an evidence-based solution to routinize intake interviews with victims petitioning the court. Language: en

This paper reviews the principal econometric models used to measure the effects of public support for firm R&D investment. A taxonomy classifying papers according to the estimation method used (system of equations versus reduced-form), type of data (cross-sectional versus longitudinal), and type of policy variable (binary versus continuous) is provided. Through a historical reconstruction of the literature, the review starts by exploring the main features of early structural models and their recent refinements, by paying special attention to the issue of subsidy endogeneity. Subsequently, it discusses the second generation of the structural approach, the Selection-models, pointing out their ability in properly describing the two-player form of an R&D incentive scheme. It then goes on by treating and discussing more data-driven approaches, such as those based on Control-function and, more specifically, Matching. Finally, we address evaluation methods rooted in dynamic models of imperfect competition, considered as a novel promising stream of research within the last generation of ‘harder’ structural approaches. A discussion of features, advantages and drawbacks of each approach in a comparative perspective is offered to the reader, as well as suggestions for future improvements in this field of economic research.