1. Preparation and evaluation of a chiral HPLC stationary phase based on cone calix[4]arene functionalized at the upper rim with l-alanine units.
- Author
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Yaghoubnejad S, Tabar Heydar K, Ahmadi SH, and Zadmard R
- Subjects
- Dinitrobenzenes chemistry, Models, Chemical, Pharmaceutical Preparations analysis, Pharmaceutical Preparations isolation & purification, Stereoisomerism, Alanine chemistry, Calixarenes chemistry, Chromatography, High Pressure Liquid methods, Phenols chemistry
- Abstract
Here we report a new chiral stationary phase (CSP) immobilized on silica gel based on cone calix[4]arene functionalized at the upper rim with two l-alanine units as new chiral selector that has been used in high-performance liquid chromatography. The CSP was prepared by covalently bonding the allyl groups at the lower rim of calix[4]arene to silica gel by thiol-ene click chemistry reaction. Elemental analysis of the CSP showed that 120 μmol of chiral selector bonded per gram of silica gel. 1-Hexene was used for end-capping of unreacted mercapto groups on silica gel. Since the CSP is chemically bonded to the silica, it can be used in the normal-phase and reversed-phase mode and with halogenated solvents as mobile phases, if desired. The chromatographic performance of the CSP was evaluated in the enantioseparation of the 3,5-dinitrobenzoyl derivatives of some amino acids, diclofop-methyl and dl-mandelic acid., (Copyright © 2017 John Wiley & Sons, Ltd.)
- Published
- 2018
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