1. Microelectrochemical radioiodination of monoclonal antibody: A preliminary study
- Author
-
I. Boxen, F. W. Teare, Z. M. Wong, C. S. Kwok, S. K. Liao, P. C. Kwong, and B. M. Bowen
- Subjects
Reaction conditions ,biology ,medicine.drug_class ,Chemistry ,Organic Chemistry ,Halogenation ,chemistry.chemical_element ,Monoclonal antibody ,Iodine ,Biochemistry ,Molecular biology ,Analytical Chemistry ,Titer ,Hemadsorption ,Drug Discovery ,medicine ,biology.protein ,Radiology, Nuclear Medicine and imaging ,Antibody ,Spectroscopy - Abstract
The optimal reaction conditions for the microelectrochemical iodination of immunoglobulins were determined with non-specific human serum immunoglobulins. These conditions were used for the efficient radioiodination of a monoclonal antibody, 140.240, in submilligram quantities. An approximately five-fold decrease in the titre of the antibody against melanoma cells, as determined by the miniaturized mixed hemadsorption assay, was observed after iodination with an average of 0.85 atoms of iodine per molecule of antibody.
- Published
- 1987
- Full Text
- View/download PDF