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6 results on '"Azofra J"'

1. Detection of allergens in a cat pelt extract

Author

Azofra J

Subjects
Allergy, Immunology, Cross Reactions, medicine.disease_cause, Immunoglobulin E, Radioallergosorbent Test, Allergen, otorhinolaryngologic diseases, medicine, Animals, Humans, Immunology and Allergy, In patient, Positive skin test, Skin, Gel electrophoresis, Lymphokines, biology, Tissue Extracts, Prostatic Secretory Proteins, Allergens, medicine.disease, Molecular biology, respiratory tract diseases, Molecular Weight, Electroelution, Cats, biology.protein, Electrophoresis, Polyacrylamide Gel, Cat allergen
Abstract

Allergenic components of cat pelt extract fractionated by SDS-polyacrylamide gel electrophoresis (PAGE) and transferred to nitrocellulose membranes were identified using sera from 15 allergic patients who showed positive skin test and RAST to cat extract. Two components showed the highest IgE-binding frequency; 14 kD (being recognized by 86% of the sera examined) and 29 kD (73% of the sera tested). Both components were purified by electroelution and subjected to RAST inhibition assays. The 29 kD fraction was able to bind specific IgE to D. pteronyssinus and inhibited a RAST assay of 29 kD component (Der p I) purified from a D. pteronyssinus extract. These results could explain the finding of positive skin test with some commercial cat allergen solutions in patients with D. pteronyssinus allergy.

Published
1992

6. Autologous preparations rich in growth factors promote proliferation and induce VEGF and HGF production by human tendon cells in culture.

Author

Anitua E, Andía I, Sanchez M, Azofra J, del Mar Zalduendo M, de la Fuente M, Nurden P, and Nurden AT

Subjects
Cell Proliferation, Cells, Cultured, Culture Media, Humans, Neovascularization, Physiologic, Blood Platelets physiology, Hepatocyte Growth Factor biosynthesis, Tendons cytology, Tendons metabolism, Vascular Endothelial Growth Factor A biosynthesis, Wound Healing
Abstract

Blood platelets become activated and aggregate at the site of vessel injury. Upon activation by thrombin, platelets release storage pools of proteins and growth factors (GFs), including those involved in tissue repair. Our goal was to evaluate the potential beneficial effect of proteins released from platelet-rich clots on tendon healing. PDGF, TGF-beta-1, IGF-I, HGF, VEGF and EGF were measured in human platelet-poor plasma (PPP) and in the releasates collected from either platelet-poor or platelet-rich clots prepared in vitro. We then studied the effects of the releasates on human tendon cells in culture. Releasates from both platelet-rich and platelet-poor clots stimulated tendon cell proliferation, in contrast to un-clotted PPP. The mitogenic activity of the supernatants was not decreased by the thrombin inhibitor, hirudin. Cultured tendon cells synthesise VEGF and HGF in the presence of PPP-clots and PRP-clot releasates, thus the synthesised amount was significantly higher with supernatants from platelet-rich clots than supernatants from a platelet-poor clot (p < 0.05). These results suggest that administering autologous platelet-rich clots may be beneficial to the treatment of tendon injuries by inducing cell proliferation and promoting the synthesis of angiogenic factors during the healing process.

Published
2005
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