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Four types of zinc (Zn)-doped hydroxyapatite (Zn-HAp) nanoparticles were prepared using calcium nitrate tetrahydrate as an anti-sintering agent during calcination at 600°C for 1 hr, to prevent calcination-induced aggregation. The Zn content of the nanopowders was determined at 0, 4.3, 9.2, and 14.7% [Zn/(Ca + Zn) × 100] using inductively coupled plasma atomic emission spectroscopic analysis. Based on X-ray diffraction analysis, the products were shown to possess an apatite structure without other crystalline impurities. The cell parameters of Zn-HAp nanoparticles decreased with increasing of Zn content in the HAp structures. This tendency implies that Zn ions substituted for Ca sites in the HAp crystal lattices. To investigate the biological effects of Zn-HAp nanoparticles, cell proliferation activity of MC3T3-E1 osteoblasts and antibacterial activity against Escherichia coli were evaluated in vitro. According to the results obtained, Zn-HAp nanoparticles containing of 14.7% Zn ions was noticeable shown shareability of the conflicting activities at 0.1 mg/mL.

AIM To fabricate and characterize nano-hydroxyapatite (nHA) grafted and non-grafted glass fibre-based endodontic posts. METHODOLOGY Experimental glass fibre posts were fabricated using silanized nHA grafted (ex-HA) and non-grafted glass fibre (ex-P) reinforced resins. The structural analysis and morphological patterns were analysed with Fourier transform infrared spectroscopy and scanning electron microscopy, respectively. EverStick® glass fibre posts (eS) were used as a control group. The degree of conversion, flexural strength, and flexural modulus was investigated and the fractured structure was evaluated with a scanning electron microscope. Root canals were prepared in human extracted teeth restored with experimental and control posts. The push-out bond strength was evaluated with radicular dentine at days 7, 30, and 90, and the presence of voids at the interface were measured at day 1, 7, 30, and 90 with micro-computed tomography. The Shapiro-Wilk test and one-way ANOVA post-hoc Tukey's test were performed. The level of significance was set at 0.05. RESULTS The SEM and FTIR confirmed the presence of a silane-coupling agent on the glass fibres. The ex-HA post had a significantly lower degree of conversion compared to the ex-P post (p = .0008), but a significantly higher conversion than the eS post (p = .0014). The maximum flexural strength value was obtained with the ex-HA post with an insignificant difference (p = .366) compared to ex-P post and a significant difference (p = .029) compared to the eS post. The flexural modulus of ex-HA, ex-P, and eS posts were significantly different (p = .037). Similarly, the ex-HA post had a significantly higher push-out bond strength at days 7 and 30 (p = .037) compared to the ex-P and eS posts. The volume of voids had a nonlinear behaviour amongst the groups with no significant difference between the posts. CONCLUSION The fabrication of the experimental posts was successful and the ex-HA post had greater flexural strength and push-out bond strength compared to the ex-P post. The degree of conversion of the ex-HA post was lower than the ex-P and eS posts. The volume of voids of ex-HA and ex-P posts was lower than that of eS posts.

Grit-blasted/acid-etched titanium dental implants have a moderately roughened surface that is suitable for cell adhesion and exhibits faster osseointegration. However, the roughened surface does not always maintain stable fixation over a long period. In this study, a simple heat treatment at 600°C was performed on a commercially available dental Ti implant with grit-blasting/acid-etching, and its effect on mineralization capacity was assessed by examining apatite formation in a simulated body fluid (SBF). The as-purchased implant displayed a moderately roughened surface at the micrometer scale. Its surface was composed of titanium hydride accompanied by a small amount of alumina particles derived from the grit-blasting. Heat treatment transformed the titanium hydride into rutile without evidently changing the surface morphology. The immersion in SBF revealed that apatite formed on the heated implant at 7 days. Furthermore, apatite formed on the Ti rod surface within 1 day when the metal was subjected to acid and heat treatment without blasting. These indicate that apatite formation was conferred on the commercially available dental implant by simple heat treatment, although its induction period was slightly affected by alumina particles remaining on the implant surface. The heat-treated implant should achieve stronger and more stable bone bonding due to its apatite formation.

Enamel, once formed, loses the ability to regenerate due to the loss of the formative ameloblasts. It is subjected to constant damaging events due to exposure to external agents and oral microbiomes. An enamel remineralization process targets to replenish the lost ionic component of the enamel through a multitude of methods. Enamel remineralization is highly challenging as it has a complex organized hierarchical microstructure. Hydroxyapatite nanocrystals of the enamel vary in size and orientation along alignment planes inside the enamel rod. The inability of the enamel to remodel unlike other mineralized tissues is another substantial deterrent. One of the well-known biomaterials, bioglass (BG) induces apatite formation on the external surface of the enamel in the presence of saliva or other physiological fluids. Calcium, sodium, phosphate, and silicate ions in BG become responsive in the presence of body fluids, leading to the precipitation of calcium phosphate. Studies have also demonstrated the bactericidal potential of BG against Streptococcus mutans biofilms. The anticariogenicity and antibacterial activity were found to be enhanced when BG was doped with inorganic ions such as F, Ag, Mg, Sr, and Zn. Due to the versatility of BG, it has been combined with a variety of agents such as chitosan, triclosan, and amelogenin to biomimic remineralization process. Key strategies that can aid in the development of contemporary enamel remineralization agents are also included in this review.

Apatite has been widely used for assessing the volatile inventory and hydrothermal fluid compositions of asteroidal and planetary bodies. We report the OH, F, and Cl abundances, as well as the hydrogen isotope composition, of apatite in the CM1-2 chondrite Boriskino and in the C1-ungrouped Bench Crater meteorite. Apatite in both meteorites are halogen-poor, close to the hydroxylapatite end-member composition, and characterised by average δDSMOW values of 233 ± 92 % and -226 ± 59 ‰, respectively. Compared to apatite, the matrix in Bench Crater is depleted in D with a δDSMOW value of -16 ± 119 ‰. Comparing apatite and water H isotope compositions yields similar apatite-water D/H fractionation ΔDApatite-Water of ca. 120 - 150 ‰ for both chondrites, suggesting that apatite formed at similar temperatures. Combining a lattice strain partitioning model with apatite F and Cl abundances in Boriskino and Bench Crater yields low F and Cl abundances < 300 μg.g-1 in apatite-forming fluids, and fluid F/Cl ratios that are roughly consistent with the bulk F/Cl ratios of other CI and CM chondrites. This suggests that hydrothermal alteration on these meteorite parent bodies took place under closed-system conditions. Based on the OH abundance estimates for the apatite-forming fluids, we estimated the pH values of alteration fluids to be of ca. 10 - 13. Such alkaline fluid compositions are consistent with previous modelling and suggest that apatite formed late, towards the end of completion of hydrothermal alteration processes on the Boriskino and Bench Crater parent bodies.

Although biomineralized skeletal elements dominate the Phanerozoic fossil record, they did not become common until ~550-520 Ma when independent acquisitions of biomineralization appeared in multiple lineages of animals and a few protists (single-celled eukaryotes). Evidence of biomineralization preceding the late Ediacaran is spotty aside from the apatitic scale microfossils of the ~811 Ma Fifteenmile Group, northwestern Canada. Here, we describe scale-shaped microfossils from four vase-shaped microfossil (VSM)-bearing units of later Tonian age: the Togari Group of Tasmania, Chuar and Pahrump groups of southwestern United States, and the Roaldtoppen Group of Svalbard. These scale-shaped microfossils consist of thin, ~13 micron-long plates typically surrounded by a 1-3 micron-thick colorless envelope; they are found singly and in heterotypic and monotypic clusters of a few to >20 specimens. Raman spectroscopy and confocal laser scanning microscopy indicate these microfossils are composed of apatite and kerogen, just as is seen in the Fifteenmile Group scale microfossils. Despite compositional similarity, however, these scales are probably not homologous, representing instead, an independent acquisition of apatite mineralization. We propose that these apatite-kerogen scale-shaped microfossils are skeletal elements of a protistan cell. In particular, their consistent co-occurrence with VSMs, and similarities with scales of arcellinid testate amoebae, a group to which the VSMs are thought to belong, suggest the possibility that these microfossils may be test-forming scales of ancient arcellinid testate amoebae. The apparent apatite biomineralization in both these microfossils and the Fifteenmile scales is unexpected given its exceedingly rare use in skeletons of modern protists. This modern absence is attributed to the extravagance of using a limiting nutrient in a structural element, but multiple occurrences of apatite biomineralization in the Tonian suggest that phosphorus was not a limiting nutrient for these organisms, a suggestion consistent with the idea that dissolved seawater phosphate concentrations may have been higher at this time.

This study aimed to determine the volatile content (Cl, F, OH) and hydrogen isotope (D/H) ratios of apatite grains within the Martian meteorite NWA 11522, a paired stone of the ungrouped polymict breccia NWA 7034. Apatite F:Cl:OH ratios were measured via SEM‐EDS analyses, and found to be strikingly similar in all grains, and dominated by Cl. Apatite D/H ratios were measured in situ via the Cameca ims 1280 SIMS at the University of Hawai'i. Results varied between δD values of 782 and 52 ‰ and between water contents of 0.127 and 0.510 wt%. The data form a mixing line between two endmembers. The first endmember, a high D/H ratio and low water content endmember, represents a fluid present during the thermal event that lithified the breccia at 1.5 Ga, resetting apatite volatile content, D/H ratio, and U‐Pb ages at this time. The D/H ratio of this fluid suggests that it was derived from the crust/cryosphere (e.g., melted groundwater ice). The second endmember, a low D/H ratio and high water content endmember, represents a second Martian fluid that interacted with the breccia after lithification. The low D/H ratio of this later fluid indicates it was derived from the deeper Martian interior, and may be evidence of an impact‐related hydrothermal system on Mars during the Amazonian period. The presence of these fluids within NWA 11522 suggests that subsurface impact crater environments were still host to liquid water during the past 1.5 Ga on Mars, and still could be to this day.

In this study, mechanical properties of bioactive glass (BAG) synthetic bone graft substitute was improved by containing ZrO2 (ZrO2 -BAG), while maintaining advantageous biological properties of BAG such as osteoinductive and osteoconductive properties. The ZrO2 -BAG was produced by adding ZrO2 in the following proportions to replace Na2 O in 45S5 BAG: 1% (Zr1-BAG), 3% (Zr3-BAG), 6% (Zr6-BAG), and 12% (Zr12-BAG). Properties including XRD, XPS, SEM, DSC, fracture toughness, and Vickers microhardness were evaluated. To assess the biological properties, Ca/P apatite formation, ion release, degradation rate, cell proliferation, ALP activity (ALP), and alizarin red S staining assay (ARS) were evaluated. Also, expression of osteogenic differentiation markers, Osteopontin (OPN), confirmed by immunofluorescence staining. Finally, an in vivo test was carried out to by implanting ZrO2 -BAG into the subcutaneous tissue of rats. The results of each test were statistically analyzed with one-way ANOVA followed by Tukey's post hoc statistical test. Amorphous ZrO2 -BAG was successfully produced with increased mechanical properties as the ZrO2 content was increased. Additionally, ZrO2 -BAG exhibited a slower ion release and degradation rate compare to BAG without ZrO2 . Bioactivity of ZrO2 -BAG was confirmed with apatite layer formed on the surface, significantly higher proliferation rate and significantly enhanced ALP and the degree of ARS of the cells compare to respective controls. The tissue reactions observed in the in vivo study showed neo-formed vessels after implantation of ZrO2 -BAG.

Poly (lactate-co-glycolate) (PLGA) is a typical biocompatible and biodegradable synthetic polymer. The addition of TiO2 nanoparticles has shown to improve compressive modulus of PLGA scaffolds and reduced fast degradation. A novel method has been applied to fabricate PLGA/TiO2 scaffolds without using any inorganic solvent, with aim of improving the biocompatibility, macroscale morphology, and well inter-connected pores efficacy: Air-Liquid Foaming. Field Emission Scanning Electron Microscopy (FESEM) revealed an increase in interconnected porosity of up to 98%. As well the compressive testing showed enhancement in modulus. Bioactivity and in vitro degradation were studied with immersion of scaffolds in Simulated Body Fluid (SBF) and incubation in Phosphate Buffered Saline (PBS), respectively. Formation of apatite layer corroborated the bioactivity after soaking in SBF. Degradation rate of scaffolds was increased with excessive addition of TiO2 contents withal. The in vitro cultured human-like MG63 ostoblast cells showed attachment, proliferation, and nontoxcitiy in contact, using MTT assay [3-(4, 5-Dimethylthiazol-2-yl)-2, 5-Diphenyltetrazolium Bromide]. According to the results, the novel method utilized in this study generated porous viable tissue without using any inorganic solvent or porogen can be a promising candidate in further treatment of orthopedic patients effectively.

Owing to its antiresorptive properties, zoledronic acid (ZOL) is commonly used in the management of benign as well as malignant bone diseases. This molecule targets sites where bone is actively remodeling, and high concentrations have been reported in the jaw. The purpose of this study was to investigate whether treatment of male rats with ZOL, at a dosage equivalent to that used for antitumor treatment, impacts the short-term qualitative properties of mandibular bone independent of bone remodeling. Thirty rats were randomly assigned to treatment either with ZOL or with serum-vehicle (control) (weekly injections: 100 μg kg-1 for 6 wk, n = 15 per group). Using the tetracycline double-labeling technique, remodeled bone areas, corresponding to the preferential site of bisphosphonate binding, were found in the alveolar bone along the alveolar bone proper. The composition of bone in these areas was characterized using Raman microspectroscopy and compared with adjacent, non-remodeled, older bone. The ZOL-treated group exhibited higher crystallinity in the remodeled bone areas (+2%), reflecting an early maturation of the apatite mineral after ZOL injection. Our findings highlight a direct and rapid effect of clinically relevant anti-tumoral ZOL doses on the qualitative properties of mandibular bone, especially on mineral crystallinity in the vicinity of the teeth, namely, the alveolar bone proper.

Bone is a self-engineered structural component of the human body with multifaceted mechanical strength, which provides indomitable support to the effective functioning of the human body. It is indispensable to find a suitable biomaterial for substituting the bone as the bone substitute material requirement is very high due to the rate of bone fracture and infection lead to osteoporosis in human beings increases rapidly. It is not an easy task to design a material with good apatite deposition ability, a faster rate of dissolution, superior resorbability, high mechanical strength, and significant bactericidal activity. Since the synthetic hydroxyapatite was not able to achieve the dahlite phase of hydroxyapatite (natural bone mineral phase), silicates emerged as an alternate biomaterial to meet the need for bone graft substitutes. All silicates do not exhibit the properties required for bone graft substitutes, as their composition and methodology adopted for the synthesis are different. Calcium, magnesium, and silicon play a major role in the formation of bone mineral and their metabolism during bone formation. In this review, the relationship between composition and activity of calcium, magnesium-based silicates have been discussed along with the future scope of these materials for hard tissue engineering applications.

Three-dimensional (3D) scaffolds with interconnected, hierarchically structured pores, and biomimetic nanostructures are desirable for tissue engineering, where preparation with a facile and biocompatible strategy remains challenging. In the present work, an innovative porous microspheres-aggregated 3D PCL scaffold with macropores, micropores, and nanofibrous-like structures was fabricated through a one-pot thermally induced phase separation (TIPS) method without the use of any porogen or specific instruments. Importantly, the porosity, pore size, and mechanical properties of our scaffolds were tailorable through tuning of the polymer concentration. Interestingly, the bioactivity of our 3D PCL scaffolds was significantly improved, as abundant apatite-like layers were formed on the 3D porous scaffolds, while no obvious apatite was observed on the 2D flat PCL film. Moreover, the high surface area attributed to the hierarchical macro/micro/nanostructure enabled our 3D porous scaffold to serve as a drug delivery depot for sustained release of both small molecule drug (phenamil) and protein (BMP2). In addition to sustained drug release, the hierarchical structure and high mechanical properties also contribute to significantly improving BMP2-induced osteogenic differentiation. In summary, we developed a novel PCL porous scaffold through a facile, one-pot TIPS method and demonstrated its promising potential application in large bone defect repair.

In this present study, in vitro characterization on bioactivity and biocompatibility of the copper-doped hydroxyapatite (Cu-HA) coatings deposited onto Ti6Al4V alloy with increasing copper content obtained using solution precursor plasma spray process were evaluated under simulated body fluid (SBF) and cytotoxicity tests. The growth of flake-like structures was observed at the coatings' surface after 7 days of immersion in SBF. Elemental composition analysis showed a calcium-deficient carbonate-containing apatite for the grown layer. Broadening of the HA peaks were also observed in the X-ray diffraction patterns and infrared absorption spectra of the immersed coatings associated with the possible formation of an amorphous layer at the surface of the coatings. Copper incorporation does not alter the bioactivity of HA but only slowed down for 10 mol% copper content. Cytotoxicity evaluation using MTT assays showed that 3 mol% Cu-HA coating was not toxic under in vitro characterizations.

To solve the lack of strength of calcium phosphate ceramic coatings in load-bearing applications, gradient Ca-P bioceramic coatings doped with La2 O3 and SiO2 are fabricated by laser cladding on Ti-6Al-4 V. The effect of SiO2 on microstructure, microhardness, bioactivity, and biocompatibility of coatings was investigated. The experimental results illustrate that the coating doped with La2 O3 and SiO2 has excellent metallurgical bonding. The XRD analysis confirms that the amount of hydroxyapatite and tricalcium phosphate in the coating reached maximum when doping amount of SiO2 is 10 wt %. SiO2 -doped coatings show a significantly higher bone-like apatite precipitation after immersion in SBF than that of other coatings. in vitro experiment also shows that coating with 10 wt % SiO2 is more suitable for the attachment and proliferation of MG63 cells, indicating that coating with 10 wt % SiO2 exhibits best bioactivity and biocompatibility. These results suggest that the addition of SiO2 improves the bonding strength, bioactivity, and biocompatibility of coatings.

We performed continuous ramped heating (CRH) on apatites from various tectonic settings and found two major types of 4He outgassing behavior. Apatites with good (U–Th)/He age reproducibility show s...

Apatite – Ca5(PO4)3(OH,F,Cl) – is a common accessory mineral in many terrestrial rocks and has a low (~75˚C) closure temperature for He retention[1]. As such the (U-Th-Sm)/He thermochronometer has ...

Apatite (U-Th)/He (AHe) thermochronology depends on accurate knowledge of how diffusion occurs. This involves measurement of core diffusion kinetics as well as understanding the behavior of migrati...

We report a new series of step-etch experiments to reveal the influence of microscopy technique on track selection bias. Two different aliquots of induced tracks in Durango apatite were etched for ...

Factors influencing data reproducibility of fission-track (FT) thermochronology can be summarized into three main categories associated with data acquisition steps. (1) Sample preparation involves ...

Although mineral dissolution is a fundamental soil forming process, primary mineral content of soils is rarely inventoried or quantified. Concerns about maintenance of forest soil nutrient stocks require better mineralogic data. In particular, to understand recovery from Ca losses induced by acid deposition and sustainability of intensive forest harvest, better inventory of primary Ca minerals is needed. We developed a method based on electron probe micro-analysis to quantify primary mineral concentrations in soil. Samples analyzed from three sites in the glaciated northeastern United States spanned a bulk Ca concentration of 0.03 to 0.94%. Plagioclase was the dominant Ca-bearing mineral at two sites while apatite was the only Ca-bearing mineral found in all three samples and was the dominant calcium source at one site. A variety of other Ca silicate minerals were found in trace amounts. Electron probe microanalysis (EPMA) proved more sensitive in detecting minor and trace Ca bearing minerals than standard XRD procedures. The Ca-bearing minerals detected by EPMA matched those expected based on the lithologic composition of the glacial till and were consistent with measured bulk soil Ca content by lithium-borate fusion. Taking into account the Ca concentration in each mineral and each mineral's abundance in the sample, this inventory provided a complete accounting of Ca sources.