1. Ameloblastoma driver mutations revealed by next‐generation sequencing of formalin‐fixed paraffin‐embedded specimens (1048.18)
- Author
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Justin I. Odegaard, Robert J. Pelham, Sushama Varma, Jonathan R. Pollack, Kevin A. Kwei, Andrew C. McClary, Philip A. Beachy, Carol D. Jones, Lila Neahring, James L. Zehnder, Kunbin Qu, Tony Ng, Brian P. Rubin, Jewison Biscocho, Xu Gong, Megan L. Troxell, Robert T. Sweeney, Robert B. West, and Benjamin R. Myers
- Subjects
Sanger sequencing ,Somatic cell ,Cancer ,Computational biology ,Biology ,medicine.disease ,Biochemistry ,DNA sequencing ,symbols.namesake ,Genetics ,symbols ,medicine ,Ameloblast ,Ameloblastoma ,Molecular Biology ,Hedgehog ,Exome sequencing ,Biotechnology - Abstract
Rare cancer types are not only understudied, but are typically represented by formalin-fixed paraffin-embedded (FFPE) (rather than freshly-frozen) specimens that are suboptimal for genomic analysis. Ameloblastoma is one such rare tumor type, thought to arise from ameloblasts, the cells that deposit enamel during tooth development. Though typically benign, ameloblastomas are locally destructive to the jaw and face, and new non-surgical interventions are needed. To discover novel driver mutations and therapeutic targets, we optimized methods and performed whole-transcriptome sequencing and/or targeted exon sequencing (TruSeq Cancer Panel) of 8 FFPE cases. Identified mutations were verified, and then evaluated on a larger, independent set of 22 FFPE cases by PCR and Sanger sequencing. From the analysis, we identified recurrent somatic mutations in three key developmental or signaling pathways, including Hedgehog, fibroblast growth factor, and MAP kinase pathways. Functional interrogation of a novel Hedgehog ...
- Published
- 2014