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At present, there is unclear evidence on the need for a negative SARS‐CoV‐2 RT‐PCR for donors with a history of COVID‐19 prior to transplantation 1,2. We report two cases of heart and liver recipients from a donor with previous documented COVID‐19 and positive RT‐PCR at time of transplantation.

The dichotomy between mechanical performance and repairability is a well-known fact in the self-healing field. One alternative to overcome this trade-off is the inclusion of reinforcing fillers. In this research, hybrid reinforced self-healing styrene-butadiene rubber (SBR) composites are developed by combining mechano-chemically modified ground tire rubber (mGTR) with carbon black (CB). The SBR matrix is systematically analyzed by means of dynamic mechanical analysis and broadband dielectric spectroscopy. The SBR composite reinforced with 20 phr mGTR and 20 phr CB maintains the healing efficiency of the unfilled SBR (80%) and improves its tensile strength by 300%, balancing perfectly well both properties. The healing efficiency is determined at different damage levels. The damage at the microscopic scale is easier to recover and does not depend on the presence of fillers, while the recovery at the macroscopic level clearly relies on the addition of filler, with more demanding healing conditions., This work was supported by the State Research Agency of Spain (AEI) [grant numbers PID2019-107501RB-I00, RYC-2017-22837]; the Spanish National Research Council (CSIC) [grant number PIE-202060E183]; and the Community of Madrid [grant number PEJ 2019-AI/IND-14635].

Magnetic reduced graphene oxide/nickel/platinum nanoparticles (rGO/Ni/PtNPs) micromotors for mycotoxin analysis in food samples were developed for food-safety diagnosis. While the utilization of self-propelled micromotors in bioassays has led to a fundamentally new approach, mainly due to the greatly enhanced target-receptor contacts owing to their continuous movement around the sample and the associated mixing effect, herein the magnetic properties of rGO/Ni/PtNPs micromotors for mycotoxin analysis are additionally explored. The micromotor-based strategy for targeted mycotoxin biosensing focused on the accurate control of micromotor-based operations: 1) on-the-move capture of free aptamers by exploiting the adsorption (outer rGO layer) and catalytic (inner PtNPs layer) properties and 2) micromotor stopped flow in just 2 min by exploiting the magnetic properties (intermediate Ni layer). This strategy allowed fumonisin B1 determination with high sensitivity (limit of detection: 0.70 ng mL-1 ) and excellent accuracy (error: 0.05 % in certified reference material and quantitative recoveries of 104±4 % in beer) even in the presence of concurrent ochratoxin A (105-108±8 % in wines). These results confirm the developed approach as an innovative and reliable analytical tool for food-safety monitoring, and confirm the role of micromotors as a new paradigm in analytical chemistry.

Aims To assess the cost-effectiveness of alternative smoking cessation scenarios from the perspective of the Spanish National Health Service (NHS). Design We used the European study on Quantifying Utility of Investment in Protection from Tobacco model (EQUIPTMOD), a Markov-based state transition economic model, to estimate the return on investment (ROI) of: (a) the current provision of smoking cessation services (brief physician advice and printed self-helped material + smoking ban and tobacco duty at current levels);and (b) four alternative scenarios to complement the current provision: coverage of proactive telephone calls;nicotine replacement therapy (mono and combo) [prescription nicotine replacement therapy (Rx NRT)];varenicline (standard duration);or bupropion. A rate of 3% was used to discount life-time costs and benefits. Setting Spain. Participants Adult smoking population (16+ years). Measurements Health-care costs associated with treatment of smoking attributable diseases (lung cancer, coronary heart disease, chronic obstructive pulmonary infection and stroke);intervention costs;quality-adjusted life years (QALYs). Costs and outcomes were summarized using various ROI estimates. Findings The cost of implementing the current provision of smoking cessation services is approximately (sic)61 million in the current year. This translates to 18 quitters per 1000 smokers and a life-time benefit-cost ratio of 5, compared with no such provision. All alternative scenarios were dominant (cost-saving: less expensive to run and generatedmore QALYs) from the life-time perspective, comparedwith the current provision. The life-time benefit-cost ratios were: 1.87 (proactive telephone calls);1.17 (Rx NRT);2.40 (varenicline-standard duration);and bupropion (2.18). The results remained robust in the sensitivity analysis. Conclusion: s According to the EQUIPTMODmodelling tool itwould be cost-effective for the Spanish authorities to expand the reach of existing GP brief interventions for smoking cessation, provide pro-active telephone support, and reimburse smoking cessationmedication to smokers trying to stop. Such policies wouldmore than pay for themselves in the long run.

This paper highlights the contribution of transmission electron microscopy (TEM) when combined with scanning electron microscopy and energy-dispersive X-ray microanalysis (SEM/XEDS) to characterize objects in archaeology. The application of TEM in archaeology is not yet a common tool, but it may provide data that are significant to understanding pre-Columbian gold metallurgy, specifically the gilding and silvering methods. Two gilded rods were studied using a combination of TEM and SEM/XEDS techniques. The objects were found at the Atacames archaeological site, in the Esmeraldas region, Ecuador, which was occupied between ad 750 and 1526 by the Atacames culture. The microchemical and structural results of the inner and the external gilded part of the artefacts support the hypothesis of a gold diffusion to the surface enhanced by chemical treatment with chloride-rich solutions, heating and successive annealing processes. The present study reveals that microstructural investigation by TEM provides useful information with which to investigate the techniques used to modify the chemical surface composition of pre-Columbian artefacts.

Exploring the connections between injection wells and seismic migration patterns is key to understanding processes controlling growth of fluid-injection induced seismicity. Numerous seismic clusters in Oklahoma have been associated with wastewater disposal operations, providing a unique opportunity to investigate migration directions of each cluster with respect to the injection-well locations. We introduce new directivity migration parameters to identify and quantify lateral migration toward or away from the injection wells. We take into account cumulative volume and injection rate from multiple injection wells. Our results suggest a weak relationship between migration direction and the cluster-well distances. Migration away from injection wells is found for distances shorter than 5-13 km, while an opposite migration towards the wells is observed for larger distances, suggesting an increasing influence of poroelastic stress changes. This finding is more stable when considering cumulative injected volume instead of injection rate. We do not observe any relationship between migration direction and injected volume or equivalent magnitudes.

In recent decades, the automatic study and analysis of plankton communities using imaging techniques has advanced significantly. The effectiveness of these automated systems appears to have improved, reaching acceptable levels of accuracy. However, plankton ecologists often find that classification systems do not work as well as expected when applied to new samples. This paper proposes a methodology to assess the efficacy of learned models which takes into account the fact that the data distribution (the plankton composition of the sample) can vary between the model building phase and the production phase. As opposed to most validation methods that consider the individual organism as the unit of validation, our approach uses a validation‐by‐sample, which is more appropriate when the objective is to estimate the abundance of different morphological groups. We argue that, in these cases, the base unit to correctly estimate the error is the sample, not the individual. Thus, model assessment processes require groups of samples with sufficient variability in order to provide precise error estimates.

Background Chronic Kidney Disease Epidemiology Collaboration (CKD-EPI) equations estimate glomerular filtration rate (GFR) more accurately than the Modification of Diet in Renal Disease (MDRD) equation. Hypothesis New CKD-EPI equations improve risk stratification in patients with non-ST-segment elevation acute coronary syndrome (NSTE-ACS) and provide complementary information to the Global Registry of Acute Coronary Events (GRACE) risk score. Methods We studied 350 subjects (mean age, 68 ± 12 years; 70% male) with NSTE-ACS. Estimated GFR was calculated using the MDRD and new CKD-EPI equations based on serum creatinine (SCr) and/or cystatin C (CysC) concentrations obtained within 48 hours of hospital admission. The primary endpoint was all-cause death during follow-up. Results Over the study period (median, 648 days [interquartile range, 236–1042 days]), 31 patients died (0.05% events per person-year). Decedents had poorer renal-function parameters (P < 0.001). Both CysC-based CKD-EPI equations had the highest areas under the receiver operating characteristic curve for the prediction of all-cause mortality. After multivariate adjustment, only CysC-based CKD-EPI equations were independent predictors of all-cause mortality (CKD-EPISCr-CysC, per mL/min/1.73 m2: hazard ratio: 0.975, 95% confidence interval: 0.956-0.994, P = 0.009; CKD-EPICysC, per mL/min/1.73 m2: hazard ratio: 0.976, 95% confidence interval: 0.959-0.993, P = 0.005). Reclassification analyses showed that only CysC-based CKD-EPI equations improved predictive accuracy of the GRACE risk score. Conclusions In patients with NSTE-ACS, CysC-based CKD-EPI equations improved clinical risk stratification for mortality and added complementary prognostic information to the GRACE risk score.

Background White tea infusion (Camelia sinensis) has antioxidants properties. The infusion contains polyphenols that have been proposed to induce antioxidant response element (ARE) response via nuclear factor E2-related factor 2 (NRF2). Adriamycin (ADR) has antitumour properties and oxidative effects. Oxidative stress is related to a variety of kidney diseases. Prevention of the oxidative stress through long-term intake of white tea and the study of the molecular mechanisms involved in protection could be of great interest. Rats were given distilled water, 0.015 or 0.045 g of solid white tea extract kg(-1) body weight for 12 months. Animals received an injection of ADR. In kidney, oxidative stress parameters were measured, the expressions of nuclear factor E2-related factor 2 gene (Nrf2), and detoxifying and antioxidants genes were analysed, and the activities of catalase (CAT), superoxide dismutase (SOD) and glutathione reductase (GR) were measured. Results ADR administration increased oxidative parameters and decreased the antioxidant activity; significantly increased the expression of analysed genes and the activity of CAT and SOD and decreased GR activity. The highest white tea dose protected redox status and inhibited ARE response. Conclusion Long-term intake of white tea protected kidney against the oxidative stress. ADR activated the ARE response but in animals treated with the highest dose of white tea, this response was inhibited, probably for antioxidant protection. © 2015 Society of Chemical Industry.

Summary The ‘cytometric diversity’ of phytoplankton communities has been studied based on single-cell properties, but the applicability of this method to characterize bacterioplankton has been unexplored. Here, we analysed seasonal changes in cytometric diversity of marine bacterioplankton along a decadal time-series at three coastal stations in the Southern Bay of Biscay. Shannon–Weaver diversity estimates and Bray–Curtis similarities obtained by cytometric and molecular (16S rRNA tag sequencing) methods were significantly correlated in samples from a 3.5 year monthly time-series. Both methods showed a consistent cyclical pattern in the diversity of surface bacterial communities with maximal values in winter. The analysis of the highly resolved flow cytometry time-series across the vertical profile showed that water column mixing was a key factor explaining the seasonal changes in bacterial composition and the winter increase in bacterial diversity in coastal surface waters. Due to its low cost and short processing time as compared with genetic methods, the cytometric diversity approach represents a useful complementary tool in the macroecology of aquatic microbes.

Studies on the size-scaling of phytoplankton growth rate are usually based on temperature-corrected growth rates or experiments performed at a fixed temperature, but the effects of differing thermal adaptation of small and large species have not been considered. We use an extensive dataset of phytoplankton growth rate responses to temperature and cell size to show that the unimodal size-scaling of phytoplankton growth depends strongly on temperature, and is not significant at high temperatures where the most common picophytoplankton species grow at their optimum. Furthermore, we show that the unimodality results from the different growth rate scaling of picophytoplankton, which differs phylogenetically from larger phytoplankton taxa. Using ribosomal RNA sequences we recalculated the size-scaling allometry with Phylogenetic Generalized Least Squares regression. After phylogenetic correction, the unimodal relationship is not significant at any temperature, suggesting that the observed curvature reflects the evolutionary adaptation of picophytoplankton to the warm conditions usually encountered in oligotrophic environments.

This work was funded by project Predicting the metabolic balance of the oceans (METOCA, CTM2009-13882-MAR) and Coastal Ocean Microbial communities and Temperature (COMITE, CTM2010-15840) funded by Spanish National Investigation+Development+Innovation (I+D+I) Plan.

AIMS: To estimate the frequency of alcohol consumption among nursing students and describe their behaviour patterns in relation to excessive consumption. BACKGROUND: Most alcohol-related problems appear in individuals who indulge in hazardous consumption, with hazardous drinkers constituting a priority in the field of preventive activities. According to previous studies, there is a high proportion of hazardous drinkers among university students. DESIGN: Descriptive cross-sectional study. METHODS: Over the course of the 2012-2013 academic year, we assessed 1060 nursing degree students, ascertaining their socio-demographic characteristics, lifestyle and alcohol consumption by means of the Systematic Alcohol Consumption Interview (Interrogatorio Sistematizado de Consumos Alcoholicos/ISCA) and Alcohol Use Disorders Inventory Test (AUDIT). RESULTS: Hazardous alcohol consumption was observed in 43·4% of students. Moreover, 14·9% of men and 18·7% of women met criteria for hazardous drinkers, without any statistically significant difference between the sexes. The frequency of hazardous drinkers was significantly higher among participants aged under 21 years, those living outside the family nucleus and smokers. CONCLUSION: A considerable proportion of students show evidence of hazardous alcohol consumption and, while there are no sex-related differences, the proportion of hazardous drinkers tends to be higher among the youngest subjects, smokers and persons living outside the family nucleus. Alcohol-prevention activities should envisage greater protection of university settings, particularly where future health professionals are involved. Language: en

Most oil refineries and factories producing basic chemical products are located in port areas, with the result that industrial complexes potentially have a strong environmental impact on their area of influence. These externalities result in a loss of welfare for citizens residing in neighbouring areas. In a context of sustainable development, companies must integrate concerns about the natural environment in their business strategy. External stakeholders, as residents, find it difficult to visualize the actions firms take to reduce their environmental impact, and the adoption of voluntary certified environmental management systems (EMSs) acts as a signal indicating the adequate environmental behaviour of these companies. These certifications enable companies to achieve the social legitimacy they need for long-term survival and competitiveness. In the context of a petrochemical industrial complex located in the port of Castellon (Spain), this paper primarily discusses whether such certifications – which act as signals of firms’ desirable environmental conduct – translate into higher trust in firms and lower risk perception by residents. Contrary to what might be expected, despite the widespread use by companies of voluntary and certified EMSs, the research findings confirm a relatively high citizen perception of risk regarding the industrial complex and a low trust in companies. On the other hand, the findings also show a low trust in the public institutions responsible for authorizing and monitoring firms’ activities and for enforcing possible sanctions in non-compliance cases. Copyright © 2013 John Wiley & Sons, Ltd and ERP Environment

An electrochemical screening for the determination of total biomarkers (thymol and carvacrol) of Lippia graveolens using single-walled carbon nanotubes screen-printed electrodes (SWCNT-SPEs) is proposed. The suitability of the approach was demonstrated using GC-MS by the analysis of selected Mexican oregano essential oil chemotypes. The analytical performance was excellent in terms of inter-droplet precision (RSD≤6 %), sensitivity and linearity. An electrochemical index of biomarkers is proposed for fast assessment of oregano quality using just 50 µL of the sample. The route here proposed constitutes a very convenient screening tool in decentralized analysis because of the simplicity, disposability and easy-to-use by non-specialist user.

This paper describes the causes that generate the relatively high – among comparable OECD countries – prevalence of tobacco consumption in Spain. It evaluates the current policy interventions geared towards reducing incidence and prevalence. It argues that, despite a recent major breakthrough in legislation on advertising and consumption in public places, one of the major shortcomings of current policies is the lack of an effective fiscal policy. This lack of effectiveness is explained by some idiosyncrasies of the Spanish cigarette market that call for specific measures.

Introduction: Sirolimus-eluting stents (SES) have demonstrated low target vessel revascularizations and low incidence of angiographic restenosis in several clinical scenarios. The aim of the present study was to assess the efficacy and safety of SES for the treatment of proximal left anterior descending coronary artery (pLAD) lesions. Methods: Ninety-six patients with severe pLAD stenosis were enrolled. Angiographic and clinical follow-up were performed at 6 and 24 months, respectively. Death, myocardial infarction (MI), new target lesion revascularization (TLR), and target vessel failure (TVF) were registered. Clinical, angiographic, and procedural variables were analyzed to identify predictors of restenosis. Results: Mean clinical follow-up was 858 ± 158 days (26.5 ± 8.3 months). Angiographic procedural success was 100%. Angiographic follow-up showed 8.4% of binary restenosis without edge-restenosis phenomenon. Late loss was 0.15 ± 0.65 mm; 15.6% of patients had an adverse cardiac event, with 1% of death, 5.2% of MI, 6.3% of TLR, and 9.4% of TVF. At 2 years, the probabilities of cumulative TVF- and TLR-free survival were 90.6% and 93.7%, respectively. Interestingly, no adverse cardiac events were registered between the first and second years. Female gender (OR 10.7 CI 95%[1.7–66.7]) and in-stent restenosis (OR 8.2, CI 95%[1.2–56.4]) were found as independent predictors of binary restenosis.Advanced chronic renal failure showed a strong trend toward worse outcome in terms of binary restenosis (P = 0.063). Conclusions: SES for the treatment of pLAD stenosis proved safe and effective in a long-term follow-up with low incidence of adverse cardiac events and restenosis. Female gender and in-stent restenosis were predictors of binary restenosis.

Appendicularians have some of the highest growth rates among metazoans but they are usually outnumbered by the slower growing copepods in mesozooplankton communities. We present experimental evidence that the eggs and juveniles of the appendicularian Oikopleura dioica are actively consumed by various copepod species. Clearance rates of Calanus helgolandicus, Candacia armata, Centropages typicus, Eucalanus crassus, and Temora longicornis on eggs of O. dioica were usually above 300 ml copepod-1 d-1. The ingestion rates of C. helgolandicus on O. dioica eggs did not reach saturation even at the highest egg concentrations (4,560 eggs L-1). Although C. helgolandicus, C. typicus, and C. armata preyed actively on 1-mm-long O. dioica (approximately 0.2 mm in trunk length), predatory pressure decreased with increasing appendicularian size. We suggest that the association of dense appendicularian populations with phytoplankton blooms could be explained by their opportunistic response to beneficial conditions before copepod densities become high enough to cause a decline in appendicularian populations due to predation. Our results indicate that, in addition to the direct shunt of biomass from picoplankton to fish, appendicularians can occupy a similar role to that of microzooplankton in the ocean. They represent an intermediate step in the less efficient, picoplankton–appendicularia–copepod-fish food chain.

This paper presents an econometric analysis of the effect of policy instruments such as prices, restrictions to use and health warnings on the decisions to start and quit smoking. The duration up to starting and up to quitting is represented with several parametric models for the hazard function, and statistical tests are used to choose the best specification. The estimates reveal that prices have a very weak effect on the duration up to starting. On the contrary, the duration up to quitting is shortened by increases in the prices of the cheapest varieties of cigarettes in the Spanish market. This evidence suggests specific directions for change in the structure of tobacco taxes.

Rate effects in the Hertzian contact loading of model glass/polycarbonate and silicon/polycarbonate bilayers bonded by epoxy adhesive are examined. Glass is used because of its high susceptibility to slow crack growth, making this conventional contribution to the rate dependencies easy to distinguish. Silicon is used as a control material with effectively no slow crack growth. Abrasion damage is introduced into the undersurfaces of the brittle coating layers to provide controlled flaws for the initiation of radial cracks from flexural stresses introduced by the contact loading. Critical loads are measured as a function of loading rate. Comparative flexural strength tests on free-standing abraded specimens show a pronounced rate dependence in the glass but none in the silicon, entirely consistent with slow crack growth effects. The glass/polycarbonate bilayer critical load data show a similar trend, but with stronger loading-rate dependence, suggesting an extraneous contribution to the kinetics from the adhesive/substrate. The silicon/polycarbonate bilayer data also show a loading-rate dependence, albeit much smaller, confirming this last conclusion. Data from cyclic contact tests on the glass/polycarbonate bilayers coincide with the loading-rate data on lifetime plots, eliminating the likelihood of a mechanical component in the fatigue response. It is concluded that the adhesive/substrate contribution is viscoelastic in nature, from energy-dissipating (but noncumulative) anelastic deformation during the cyclic loading. Critical load tests on bilayers with different exposures to external water show no influence of external environment, suggesting that internal moisture is responsible for the slow crack growth in the glass-coating bilayers.

An amperometric immunosensor for the detection of the herbicide atrazine has been developed. A redox polymer PVPOs(bpy)2Cl was co-immobilized with the specific antibody on the surface of the electrode by crosslinking with PEGDGE to form an electron-conducting hydrogel. In a competitive assay the occurrence of the antibody-antigen reaction on the surface of the sensing film was detected through the 'electrical wiring' of the redox centres of antigen-labelled horseradish peroxidase and the electrode surface in the presence of H2O2 at 0.1 V (vsAg/AgCl).

In this paper we estimate a demand for private medical services equation based on the tradition of the Grossman´s model of demand for health using data for a panel of Spanish households. The econometric specification accounts for the censored nature of the data, which arises from no participation and infrequency of purchases, and the existence of unobserved heterogeneity, which arises from the non observability of health stata. Our evidence suggests that ignoring these features can have a significant impact on the size, sign and significance of the model estimates. The estimates for the participation and consumption processes also suggest that the deduction of expenditures on health care currently applicable in the Spanish tax system are positively associated to income and fertility. Financial support from Merck through the Centre de Recerca d’Economia i Salut is thankfully acknowledged.

Plant resistance to chilling injury depends on the maintenance of membrane functions. An increase in the degree of unsaturation of membrane fatty acids can help maintain membrane function at low temperatures by reducing the temperature at which deleterious membrane phase-transitions occur. The fatty acid composition of membranes of the chlorophycean Caulerpa prolifera was determined in summer and winter in the Mar Menor coastal Lagoon (SE Spain). In January the percentage of polyunsaturated fatty acids was higher than in August. Membrane content of mono- and diunsaturated fatty acids was similar in the two seasons. The unsaturation index of membranes changed from 76.15 +/- 1.80 (mean +/- standard error) in August to 128.05 +/- 0.32 in January. It is suggested that this increase in the degree of unsaturation might be important to allow C. prolifera to decrease its lower threshold of survival temperature and acclimate net photosynthesis and dark respiration rates to winter temperatures in the Mar Menor.

Many macroecological theories have been developed to study the diversity on our planet. All these theories require the existence of consistent databases to test their predictions. In this work, we compiled a data set of marine microplankton species abundances at 788 stations with an extensive geographical coverage. Data were collected on different oceanographic cruises between 1992 and 2002. This database consists of abundances (cells/mL) for each species at each station and depth, together with estimates of the biomass and biovolume for each species. One of the key strengths in this database is that species identifications were made by the same taxonomist, which provides greater strength to the collection and ensures that estimates of species diversity are reliable. Environmental information has also been compiled at each station (chlorophyll, temperature, photosynthetically active radiation [PAR], nutrients) in order to have a characterization of the study area and to be used in studies on the environmental and biological controls of marine biodiversity.