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Start Over Topic histology Publisher wiley
3 results

1. Nucleic acid storage on FTA cards from cytological samples

Author

Carmine Selleri, L. Lucchese, C. Picariello, V. Caputo, Anna Lucia Peluso, and Pio Zeppa

Subjects
Paper, Histology, business.industry, Biopsy, Fine-Needle, Cytological Techniques, 030231 tropical medicine, DNA, General Medicine, Pathology and Forensic Medicine, storage, FTA cards, 03 medical and health sciences, 0302 clinical medicine, Biochemistry, Nucleic acid, Nucleic Acids, 030220 oncology & carcinogenesis, cytological samples, Humans, RNA, Medicine, Nucleic acid, storage, FTA cards, cytological samples, business
Published
2017

2. Controlled serial grinding for high-resolution three-dimensional reconstruction

Author

G, Chinga, P O, Johnsen, and O, Diserud

Subjects
Paper, Histocytological Preparation Techniques, Histology, Surface Properties, Computer science, business.industry, High resolution, Polishing, Image Enhancement, Pathology and Forensic Medicine, Grinding, Image Processing, Computer-Assisted, Microscopy, Electron, Scanning, Computer vision, Artificial intelligence, business, Image resolution, Interpolation, Volume (compression)
Abstract

Summary This work reports an improved preparation procedure for acquiring SEM images for three-dimensional (3D) reconstruction. The images are acquired consecutively after serial grinding and polishing. Ugelstad beads are used as landmarks for registration purposes and for determining the thickness of the abraded sections. An estimation of the bead sizes necessary for suitable thickness quantification at the corresponding spatial resolution and uncertainty is given. Shape-based interpolation is used for filling the gaps between the cross-sections. An indication of the distance between cross-sections necessary for good interpolation is also given. An example of a 3D reconstructed paper volume is presented. The method is suitable for preparation of fibre and paper as well as other materials.

Published
2004

3. A simple method for the handling and orientation of small specimens for electron microscopy

Author

Robert M.K.W. Lee, J. B. Forrest, and J. Carson

Subjects
Paper, Histology, Materials science, Orientation (computer vision), Lens (geology), Nanotechnology, Specimen Handling, Pathology and Forensic Medicine, law.invention, Microscopy, Electron, Nasal Mucosa, law, Adhesives, Humans, Cilia, Electron microscope, Biomedical engineering, Fixation (histology)
Abstract

Cyanoacrylic glue (Eastman 910) was used to affix small pieces of nasal scrapings to lens paper immediately before fixation in the glutaraldehyde. The lens paper not only served to hold specimens together so that they were not lost during tissue processing, but also functioned as a 'landmark' for the specimens, so that specimens could be oriented in a specific manner during embedding and subsequent sectioning.

Published
1982