1. Identifying polymorphic regions of the p190 protein from different Plasmodium falciparum strains by using specific T cells
- Author
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R. H. Meloen, Gabriele Süss, J. R. L. Pink, Hugues Matile, and Bela Takacs
- Subjects
Protozoan Vaccines ,Serotype ,Cellular immunity ,T-Lymphocytes ,Molecular Sequence Data ,Plasmodium falciparum ,Immunology ,Protozoan Proteins ,Antigens, Protozoan ,Cross Reactions ,Lymphocyte Activation ,Epitope ,Plasmodium chabaudi ,Epitopes ,Mice ,Immune system ,Antigen ,parasitic diseases ,Animals ,Humans ,Amino Acid Sequence ,Protein Precursors ,Merozoite Surface Protein 1 ,Mice, Inbred BALB C ,Mice, Inbred C3H ,Polymorphism, Genetic ,Sequence Homology, Amino Acid ,biology ,Malaria vaccine ,Vaccination ,biology.organism_classification ,Virology ,Recombinant Proteins ,Mice, Inbred C57BL ,Female ,Parasitology ,Sequence Alignment - Abstract
The p190 protein (also called MSA1 or MSP1) of the asexual blood stage forms of Plasmodium falciparum, a human malaria vaccine candidate, shows polymorphism between different isolates. Mice were immunized with p190-3, a recombinant protein which contains mostly conserved sequences derived from the p190 protein of the K1 parasite isolate. Proliferative T-cell responses of lymph node cells from immunized mice were assessed by stimulation in vitro with p190-3 or preparations of parasitized red blood cells (PRBC) containing the native protein. The p190-3-specific T cells from C57BL/6 mice consistently responded to some P. falciparum isolates, representing either the K1 or MAD20 serotype of p190, but not to other P. falciparum strains or to rodent malaria parasite-infected red blood cells. p190-3-specific T-cell responses from other mouse strains (BALB/c, C3H/He) did not distinguish between P. falciparum isolates. The polymorphic epitopes which were preferentially recognized by T cells from C57BL/6 mice were identified.
- Published
- 1993
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