6 results on '"Brockow K"'
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2. Peripheral blood eosinophils and other non-invasive biomarkers can monitor treatment response in eosinophilic oesophagitis
- Author
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Schlag, C., primary, Miehlke, S., additional, Heiseke, A., additional, Brockow, K., additional, Krug, A., additional, von Arnim, U., additional, Straumann, A., additional, Vieth, M., additional, Bussmann, C., additional, Mueller, R., additional, Greinwald, R., additional, and Bajbouj, M., additional
- Published
- 2015
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3. Research needs in allergy: an EAACI position paper, in collaboration with EFA
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Papadopoulos Nikolaos G, Agache Ioana, Bavbek Sevim, Bilo Beatrice M, Braido Fulvio, Cardona Victoria, Custovic Adnan, deMonchy Jan, Demoly Pascal, Eigenmann Philippe, Gayraud Jacques, Grattan Clive, Heffler Enrico, Hellings Peter W, Jutel Marek, Knol Edward, Lötvall Jan, Muraro Antonella, Poulsen Lars K, Roberts Graham, Schmid-Grendelmeier Peter, Skevaki Chrysanthi, Triggiani Massimo, vanRee Ronald, Werfel Thomas, Flood Breda, Palkonen Susanna, Savli Roberta, Allegri Pia, Annesi-Maesano Isabella, Annunziato Francesco, Antolin-Amerigo Dario, Apfelbacher Christian, Blanca Miguel, Bogacka Ewa, Bonadonna Patrizia, Bonini Matteo, Boyman Onur, Brockow Knut, Burney Peter, Buters Jeroen, Butiene Indre, Calderon Moises, Cardell Lars, Caubet Jean-Christoph, Celenk Sevcan, Cichocka-Jarosz Ewa, Cingi Cemal, Couto Mariana, deJong Nicolette, Del Giacco Stefano, Douladiris Nikolaos, Fassio Filippo, Fauquert Jean-Luc, Fernandez Javier, Rivas Montserrat, Ferrer Marta, Flohr Carsten, Gardner James, Genuneit Jon, Gevaert Philippe, Groblewska Anna, Hamelmann Eckard, Hoffmann Hans, Hoffmann-Sommergruber Karin, Hovhannisyan Lilit, Hox Valérie, Jahnsen Frode L, Kalayci Ömer, Kalpaklioglu Ayse, Kleine-Tebbe Jörg, Konstantinou George, Kurowski Marcin, Lau Susanne, Lauener Roger, Lauerma Antti, Logan Kirsty, Magnan Antoine, Makowska Joanna, Makrinioti Heidi, Mangina Paraskevi, Manole Felicia, Mari Adriano, Mazon Angel, Mills Clare, Mingomataj ErvinÇ, Niggemann Bodo, Nilsson Gunnar, Ollert Markus, O'Mahony Liam, O'Neil Serena, Pala Gianni, Papi Alberto, Passalacqua Gianni, Perkin Michael, Pfaar Oliver, Pitsios Constantinos, Quirce Santiago, Raap Ulrike, Raulf-Heimsoth Monika, Rhyner Claudio, Robson-Ansley Paula, Alves Rodrigo, Roje Zeljka, Rondon Carmen, Rudzeviciene Odilija, Ruëff Franziska, Rukhadze Maia, Rumi Gabriele, Sackesen Cansin, Santos Alexandra F, Santucci Annalisa, Scharf Christian, Schmidt-Weber Carsten, Schnyder Benno, Schwarze Jürgen, Senna Gianenrico, Sergejeva Svetlana, Seys Sven, Siracusa Andrea, Skypala Isabel, Sokolowska Milena, Spertini Francois, Spiewak Radoslaw, Sprikkelman Aline, Sturm Gunter, Swoboda Ines, Terreehorst Ingrid, Toskala Elina, Traidl-Hoffmann Claudia, Venter Carina, Vlieg-Boerstra Berber, Whitacker Paul, Worm Margitta, Xepapadaki Paraskevi, and Akdis Cezmi A
- Subjects
Allergy ,Allergic diseases ,Policy ,Research needs ,Research funding ,Europe ,Immunologic diseases. Allergy ,RC581-607 - Abstract
Abstract In less than half a century, allergy, originally perceived as a rare disease, has become a major public health threat, today affecting the lives of more than 60 million people in Europe, and probably close to one billion worldwide, thereby heavily impacting the budgets of public health systems. More disturbingly, its prevalence and impact are on the rise, a development that has been associated with environmental and lifestyle changes accompanying the continuous process of urbanization and globalization. Therefore, there is an urgent need to prioritize and concert research efforts in the field of allergy, in order to achieve sustainable results on prevention, diagnosis and treatment of this most prevalent chronic disease of the 21st century. The European Academy of Allergy and Clinical Immunology (EAACI) is the leading professional organization in the field of allergy, promoting excellence in clinical care, education, training and basic and translational research, all with the ultimate goal of improving the health of allergic patients. The European Federation of Allergy and Airways Diseases Patients’ Associations (EFA) is a non-profit network of allergy, asthma and Chronic Obstructive Pulmonary Disorder (COPD) patients’ organizations. In support of their missions, the present EAACI Position Paper, in collaboration with EFA, highlights the most important research needs in the field of allergy to serve as key recommendations for future research funding at the national and European levels. Although allergies may involve almost every organ of the body and an array of diverse external factors act as triggers, there are several common themes that need to be prioritized in research efforts. As in many other chronic diseases, effective prevention, curative treatment and accurate, rapid diagnosis represent major unmet needs. Detailed phenotyping/endotyping stands out as widely required in order to arrange or re-categorize clinical syndromes into more coherent, uniform and treatment-responsive groups. Research efforts to unveil the basic pathophysiologic pathways and mechanisms, thus leading to the comprehension and resolution of the pathophysiologic complexity of allergies will allow for the design of novel patient-oriented diagnostic and treatment protocols. Several allergic diseases require well-controlled epidemiological description and surveillance, using disease registries, pharmacoeconomic evaluation, as well as large biobanks. Additionally, there is a need for extensive studies to bring promising new biotechnological innovations, such as biological agents, vaccines of modified allergen molecules and engineered components for allergy diagnosis, closer to clinical practice. Finally, particular attention should be paid to the difficult-to-manage, precarious and costly severe disease forms and/or exacerbations. Nonetheless, currently arising treatments, mainly in the fields of immunotherapy and biologicals, hold great promise for targeted and causal management of allergic conditions. Active involvement of all stakeholders, including Patient Organizations and policy makers are necessary to achieve the aims emphasized herein.
- Published
- 2012
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4. The basophil activation test differentiates between patients with wheat-dependent exercise-induced anaphylaxis and control subjects using gluten and isolated gluten protein types.
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Gabler AM, Gebhard J, Eberlein B, Biedermann T, Scherf KA, and Brockow K
- Abstract
Background: Oral food challenge using gluten and cofactors is the gold standard to diagnose wheat-dependent exercise-induced anaphylaxis (WDEIA), but this procedure puts patients at risk of an anaphylactic reaction. Specific IgE to ω5-gliadins as major allergens and skin prick tests to wheat may yield negative results. Thus, we designed a proof-of-principle study to investigate the utility of the basophil activation test (BAT) for WDEIA diagnosis., Methods: Different gluten protein types (GPT; α-, γ-, ω1,2- and ω5-gliadins, high-molecular-weight glutenin subunits [HMW-GS] and low-molecular-weight glutenin subunits [LMW-GS]) and gluten were used in different concentrations to measure basophil activation in 12 challenge-confirmed WDEIA patients and 10 control subjects. The results were compared to routine allergy diagnostics. Parameters analyzed include the percentage of CD63
+ basophils, the ratio of %CD63+ basophils induced by GPT/gluten to %CD63+ basophils induced by anti-FcεRI antibody, area under the dose-response curve and test sensitivity and specificity., Results: GPT and gluten induced strong basophil activation for %CD63+ basophils and for %CD63+ /anti-FcɛRI ratio in a dose-dependent manner in patients, but not in controls ( p < 0.001, respectively). BAT performance differed from acceptable (0.73 for LMW-GS) to excellent (0.91 for ω5-gliadins) depending on the specific GPT as evaluated by the area under the receiver operating characteristic curve. Patients showed individual sensitization profiles. After determination of the best cut-off points, ω5-gliadins and HMW-GS showed the best discrimination between patients and controls with a sensitivity/specificity of 100/70 and 75/100, respectively., Conclusion: This study shows the alternative role of BAT in better defining WDEIA and the causative wheat allergens. The best BAT parameters to distinguish WDEIA patients from controls were %CD63+ basophil values for ω5-gliadins and HMW-GS., Competing Interests: B. Eberlein received methodological and technical support from Buehlmann Laboratories AG (Schönenbuch, Switzerland). The other authors declare no competing interests., (© 2021 The Authors. Clinical and Translational Allergy published by John Wiley and Sons Ltd on behalf of European Academy of Allergy and Clinical Immunology.)- Published
- 2021
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5. Clinical and serological follow-up of patients with WDEIA.
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Christensen MJ, Eller E, Mortz CG, Brockow K, and Bindslev-Jensen C
- Abstract
The clinical threshold in wheat-dependent, exercise-induced anaphylaxis seems to be lowered in patients on wheat free diet, whereas the opposite is seen in patients on regular wheat intake. Therefore, a recommendation of wheat consumption, if considered safe to the patient based on case-history and challenge results, could be advised., Competing Interests: Competing interestsThe authors declare that they have no competing interests.
- Published
- 2019
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6. Cofactors of wheat-dependent exercise-induced anaphylaxis do not increase highly individual gliadin absorption in healthy volunteers.
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Scherf KA, Lindenau AC, Valentini L, Collado MC, García-Mantrana I, Christensen M, Tomsitz D, Kugler C, Biedermann T, and Brockow K
- Abstract
Background: In wheat-dependent exercise-induced anaphylaxis (WDEIA), cofactors such as exercise, acetylsalicylic acid (ASA), alcohol or unfavorable climatic conditions are required to elicit a reaction to wheat products. The mechanism of action of these cofactors is unknown, but an increase of gliadin absorption has been speculated. Our objectives were to study gliadin absorption with and without cofactors and to correlate plasma gliadin levels with factors influencing protein absorption in healthy volunteers., Methods: Twelve healthy probands (six males, six females; aged 20-56 years) ingested 32 g of gluten without any cofactor or in combination with cofactors aerobic and anaerobic exercise, ASA, alcohol and pantoprazole. Gliadin serum levels were measured up to 120 min afterwards and the intestinal barrier function protein zonulin in stool was collected before and after the procedure; both were measured by ELISA. Stool microbiota profile was obtained by 16S gene sequencing., Results: Within 15 min after gluten intake, gliadin concentrations in blood serum increased from baseline in all subjects reaching highly variable peak levels after 15-90 min. Addition of cofactors did not lead to substantially higher gliadin levels, although variability of levels was higher with differences between individuals (p < 0.001) and increased levels at later time points. Zonulin levels in stool were associated neither with addition of cofactors nor with peak gliadin concentrations. There were no differences in gut microbiota between the different interventions, although the composition of microbiota (p < 0.001) and the redundancy discriminant analysis (p < 0.007) differed in probands with low versus high stool zonulin levels., Conclusion: The adsorption of gliadin in the gut in healthy volunteers is less dependent on cofactors than has been hypothesized. Patients with WDEIA may have a predisposition needed for the additional effect of cofactors, e.g., hyperresponsive or damaged intestinal epithelium. Alternatively, other mechanisms, such as cofactor-induced blood flow redistribution, increased activity of tissue transglutaminase, or increases in plasma osmolality and acidosis inducing basophil and mast cell histamine release may play the major role in WDEIA., Competing Interests: The authors declare that they have no competing interests.
- Published
- 2019
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