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Objective: To investigate the status of vitamin A(VA), vitamin B2 (VB2), iron and anoxidative function in anemic and non-anemic pregnant women., Methods: 426 anemic and 36 non-anemic pregnant women were included in the study. The survey of 24-hour's diet recall of pregnant women was made to evaluate intake of iron, VB2, folic acid, etc by the nutrition software provided by Beijing 301 hospital, iron and VA were measured by Radioimmunoassay (RIA) and by high-pressure liquid chromatography. VB2 status was detected using the assay for erythrocyte glutathione reductase (Egr; EC 1.6.4.2) activity. SOD and GSH-Px activities and MDA were determined using commercial kits. Peripheral blood erythrocyte membrane fluidity was detected by using 1,6-diphenyl-1,3,5-hexatriene as a probe, the degree of fluorescence polarization (P) at 25 degrees C of disrupted cells plasma membranes were compared for a variety of systems., Results: Median intakes of protein and vitamin C met the current Chinese RNIs for pregnancy, whereas intakes of(VA) and VB2 were well below the recommendations. Intake of iron were above 90%, but the main sources of iron are vegetables. Plasma VA (1.25 micromol/L) and iron (20.57 microg/L) were lower, BGRAC (1.79) was higher than that in non-anemia group (VA 1.57 micromol/L, SF 33.16 microg/L, BGRAC 1.52). The level of plasma SOD (77.1U/ml) and the activity of GSH-Px (61.9U) were lower than those in non-anemia group (92.2U/ml, 71.6U, P < 0.05), while MDA (4.58 nmol/ml) level and erythrocyte membrane (P = 0.2622, eta = 2.7465) fluidity were higher than those non-anemia group(MDA = 3.78 nmol/ ml, P = 0.2360, eta = 2.3658)., Conclusion: Plasam VA, VB2 and iron, antioxidantcapacity and erythrocyte membrane fluidity were decreased in the anemic pregnant women.

Objective: To investigate the influences of vitamin E (VE) at different dosage on peripheral blood lymphocyte (PBL) proliferation and anti-DNA oxidative damage activities and erythrocyte membrane fluidity., Methods: 48 Wistar rats were randomly divided into four groups including control group, VE1, VE2, and VE3 groups supplemented with 7.5, 50, 200, 750 IU/kg bw x d VE, respectively. The trial lasted 8 weeks and the blood samples were collected at the end of the trial. The level of plasma VE was analyzed by fluorescent spectrometry. Plasma MDA and membrane GSH-Px were analyzed by kits. The blood erythrocyte membrane fluidity was detected by fluorescence polarization method, lymphocyte transformation rate by MTT method and DNA oxidative damage by comet assay., Results: The results showed that plasma VE levels significantly increased in VE1, VE2, and VE3 groups. Plasma MDA and erythocyte membrane GSH-Px activity in the rats in 50 IU/kg bw x d (VE1) group were (2.29 +/- 0.55) nmol/ml and (367.17 +/- 129.86) U/mg prot, respectively. P (fluorescence polarization) and eta(microviscosity), which were inversely related with membrane fluidity, in VE1 group were significantly lower. Lymphocyte transformation rate was significantly increased by 261.86%, 199.23% and 412.97% and H2O2 induced DNA damage significantly decreased compared with the control, VE2, and VE3 groups., Conclusion: It is indicated that an effective intake of VE for enhancing erythrocyte membrane fluidity, lymphocyte proliferation and DNA stability was 50 IU/kg bw x d, while too excessive intake of VE could not be found to be beneficial.

Objective: To investigate the effect of beta-carotene (P-C) supplementation on DNA oxidative and alkylation damage in rats., Methods: Rats were randomly divided into five groups. The first group was no beta-C, and the second, the third, the forth and the fifth groups were daily supplemented with 0.071, 0.355, 4.28 and 15.0 mg/(kg x d). The content of plasma VA was analyzed by fluorescent spectrometry. DNA damage and oxidative damage induced by H2O2 were detected by single-cell gel electrophoresis (SCGE) and O6-Methyl-guanine (O6-MeG) was measured by high performance capillary zone electrophoresis., Results: After 8 weeks trial, the results showed that levels of plasma retinol converted from beta-C decreased by 135 microg/L in the beta-C deficiency group, which were much lower than 276 - 306 microg/L in other four beta-C supplement groups (P < 0.01). Intrinsic damage of DNA was more severe in the group of beta-C deficiency than the groups supplemented with beta-C (P < 0.01). The lower DNA oxidative damage of lymphocyte, which was induced by H2O2, was found in 4.28 mg/(kg x d) group than deficiency and other supplement groups (P < 0.05). O6-Methyl-guanine of the supplement groups was lower than the beta-C deficient group at the 6th week and 8th week (P < 0.05). O6-Methyl-guanine did not decreasing with the dose increasing and the lowest in the 4.28mg/(kg x d) group., Conclusion: Beta-C or retinol deficiency and over intake such as 15.0 mg/(kg x d) as well might cause or promote DNA damage, and a moderate amount such as 4.280 mg/(kg x d) could improve antioxidative and decrease DNA damage effectively.