1. Methods for the determination of the interferon-induced enzyme 2'-5' oligoadenylate synthetase in mononuclear blood cells.
- Author
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Bruchelt G, Beck J, Schilbach-Stückle K, Koscielniak E, Treuner J, and Niethammer D
- Subjects
- 2',5'-Oligoadenylate Synthetase biosynthesis, Chromatography, Affinity, Enzyme Induction drug effects, Evaluation Studies as Topic, Humans, In Vitro Techniques, Leukemia, Myeloid enzymology, Leukocytes, Mononuclear drug effects, Leukocytes, Mononuclear metabolism, Poly I-C metabolism, 2',5'-Oligoadenylate Synthetase blood, Interferons pharmacology, Leukocytes, Mononuclear enzymology
- Abstract
The determination of 2'-5'-oligoadenylate synthetase in peripheral blood mononuclear cells is used as a biological response parameter during therapy with interferon and in the diagnosis of diseases related to the interferon system. In this communication, some general aspects concerning the preparation of 2'-5'-oligoadenylate synthetase from peripheral blood mononuclear cells and the incubation conditions of the 2'-5'-oligoadenylate synthetase reaction are reported. Four analytical procedures for the determination of the products formed during the 2'-5'-oligoadenylate synthetase reaction were comparatively investigated and the advantages and limitations of the assays are discussed. As an example of possible clinical application, the levels of 2'-5'-oligoadenylate synthetase were determined in the mononuclear cell fraction of peripheral blood from healthy persons as well as from children with chronic myelogenous leukaemia.
- Published
- 1987
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