1. Influence of GABA and GABA-producing Lactobacillus brevis DPC 6108 on the development of diabetes in a streptozotocin rat model
- Author
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Timothy G. Dinan, Tatiana M. Marques, Elaine Patterson, Paul D. Cotter, Catherine Stanton, Gerald F. Fitzgerald, Orla O'Sullivan, R.P. Ross, Rebecca Wall, John F. Cryan, Science Foundation Ireland, and SFI/12/RC/2273
- Subjects
0301 basic medicine ,Microbiology (medical) ,medicine.medical_specialty ,Levilactobacillus brevis ,Rat model ,Placebo ,Probiotic ,Microbiology ,streptozotocin ,gamma-Aminobutyric acid ,Diabetes Mellitus, Experimental ,law.invention ,type-1 diabetes ,Placebos ,Rats, Sprague-Dawley ,03 medical and health sciences ,law ,Diabetes mellitus ,Internal medicine ,medicine ,Animals ,Hypoglycemic Agents ,gamma-Aminobutyric Acid ,Type 1 diabetes ,Antibiotics, Antineoplastic ,biology ,Lactobacillus brevis ,Probiotics ,medicine.disease ,biology.organism_classification ,Streptozotocin ,Treatment Outcome ,030104 developmental biology ,Endocrinology ,medicine.drug ,γ-aminobutyric acid - Abstract
peer-reviewed The aim of this study was to investigate if dietary administration of γ-aminobutyric acid (GABA)-producing Lactobacillus brevis DPC 6108 and pure GABA exert protective effects against the development of diabetes in streptozotocin (STZ)-induced diabetic Sprague Dawley rats. In a first experiment, healthy rats were divided in 3 groups (n=10/group) receiving placebo, 2.6 mg/kg body weight (bw) pure GABA or L. brevis DPC 6108 (~109microorganisms). In a second experiment, rats (n=15/group) were randomised to five groups and four of these received an injection of STZ to induce type 1 diabetes. Diabetic and non-diabetic controls received placebo [4% (w/v) yeast extract in dH2O], while the other three diabetic groups received one of the following dietary supplements: 2.6 mg/kg bw GABA (low GABA), 200 mg/kg bw GABA (high GABA) or ~109 L. brevis DPC 6108. L. brevis DPC 6108 supplementation was associated with increased serum insulin levels (P0.05), compared with non-diabetic controls while all other diabetic groups displayed reduced diversity (P
- Published
- 2016