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The objective of the present study was to determine the effect of vitamin E supplementation in Tris-citric acid yolk glycerol extender on seminal and biochemical parameters of semen at the end of equilibration time (pre freezing stage) and after 24 hours freezing and thawing (post freezing stage) of semen. Significant improvement in progressive sperm motility, live spermatozoa, acrosomal integrity and MDA was observed in semen supplemented with vitamin E during pre freezing and post freezing stages. However, the release of glutamic oxaloacetic transaminase (GOT) did not differ significantly in both the groups. Thus inclusion of vitamin E in the extender prior to extension and freezing has shown improvement in post thaw quality of buffalo bull semen. [ABSTRACT FROM AUTHOR]