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Start Over Author "Boutet, Sebastien" Publisher uppsala universitet, fysikalisk kemi
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1. Ultrafast structural changes within a photosynthetic reaction centre

Author

Dods, Robert, Bath, Petra, Morozov, Dmitry, Gagner, Viktor Ahlberg, Arnlund, David, Luk, Hoi Ling, Kubel, Joachim, Maj, Michal, Vallejos, Adams, Wickstrand, Cecilia, Bosman, Robert, Beyerlein, Kenneth R., Nelson, Garrett, Liang, Mengning, Milathianaki, Despina, Robinson, Joseph, Harimoorthy, Rajiv, Berntsen, Peter, Malmerberg, Erik, Johansson, Linda, Andersson, Rebecka, Carbajo, Sergio, Claesson, Elin, Conrad, Chelsie E., Dahl, Peter, Hammarin, Greger, Hunter, Mark S., Li, Chufeng, Lisova, Stella, Royant, Antoine, Safari, Cecilia, Sharma, Amit, Williams, Garth J., Yefanov, Oleksandr, Westenhoff, Sebastian, Davidsson, Jan, DePonte, Daniel P., Boutet, Sebastien, Barty, Anton, Katona, Gergely, Groenhof, Gerrit, Branden, Gisela, Neutze, Richard, Dods, Robert, Bath, Petra, Morozov, Dmitry, Gagner, Viktor Ahlberg, Arnlund, David, Luk, Hoi Ling, Kubel, Joachim, Maj, Michal, Vallejos, Adams, Wickstrand, Cecilia, Bosman, Robert, Beyerlein, Kenneth R., Nelson, Garrett, Liang, Mengning, Milathianaki, Despina, Robinson, Joseph, Harimoorthy, Rajiv, Berntsen, Peter, Malmerberg, Erik, Johansson, Linda, Andersson, Rebecka, Carbajo, Sergio, Claesson, Elin, Conrad, Chelsie E., Dahl, Peter, Hammarin, Greger, Hunter, Mark S., Li, Chufeng, Lisova, Stella, Royant, Antoine, Safari, Cecilia, Sharma, Amit, Williams, Garth J., Yefanov, Oleksandr, Westenhoff, Sebastian, Davidsson, Jan, DePonte, Daniel P., Boutet, Sebastien, Barty, Anton, Katona, Gergely, Groenhof, Gerrit, Branden, Gisela, and Neutze, Richard

Abstract

Photosynthetic reaction centres harvest the energy content of sunlight by transporting electrons across an energy-transducing biological membrane. Here we use time-resolved serial femtosecond crystallography(1) using an X-ray free-electron laser(2) to observe light-induced structural changes in the photosynthetic reaction centre of Blastochloris viridis on a timescale of picoseconds. Structural perturbations first occur at the special pair of chlorophyll molecules of the photosynthetic reaction centre that are photo-oxidized by light. Electron transfer to the menaquinone acceptor on the opposite side of the membrane induces a movement of this cofactor together with lower amplitude protein rearrangements. These observations reveal how proteins use conformational dynamics to stabilize the charge-separation steps of electron-transfer reactions. Time-resolved serial femtosecond crystallography is used to reveal the structural changes that stabilize the charge-separation steps of electron-transfer reactions in the photosynthetic reaction centre of Blastochloris viridis on a timescale of picoseconds.

Published
2021
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2. Establishing nonlinearity thresholds with ultraintense X-ray pulses

Author

Szlachetko, Jakub, Hoszowska, Joanna, Dousse, Jean-Claude, Nachtegaal, Maarten, Blachucki, Wojciech, Kayser, Yves, Sá, Jacinto, Messerschmidt, Marc, Boutet, Sebastien, Williams, Garth J., David, Christian, Smolentsev, Grigory, van Bokhoven, Jeroen A., Patterson, Bruce D., Penfold, Thomas J., Knopp, Gregor, Pajek, Marek, Abela, Rafael, Milne, Christopher J., Szlachetko, Jakub, Hoszowska, Joanna, Dousse, Jean-Claude, Nachtegaal, Maarten, Blachucki, Wojciech, Kayser, Yves, Sá, Jacinto, Messerschmidt, Marc, Boutet, Sebastien, Williams, Garth J., David, Christian, Smolentsev, Grigory, van Bokhoven, Jeroen A., Patterson, Bruce D., Penfold, Thomas J., Knopp, Gregor, Pajek, Marek, Abela, Rafael, and Milne, Christopher J.

Abstract

X-ray techniques have evolved over decades to become highly refined tools for a broad range of investigations. Importantly, these approaches rely on X-ray measurements that depend linearly on the number of incident X-ray photons. The advent of X-ray free electron lasers (XFELs) is opening the ability to reach extremely high photon numbers within ultrashort X-ray pulse durations and is leading to a paradigm shift in our ability to explore nonlinear X-ray signals. However, the enormous increase in X-ray peak power is a double-edged sword with new and exciting methods being developed but at the same time well-established techniques proving unreliable. Consequently, accurate knowledge about the threshold for nonlinear X-ray signals is essential. Herein we report an X-ray spectroscopic study that reveals important details on the thresholds for nonlinear X-ray interactions. By varying both the incident X-ray intensity and photon energy, we establish the regimes at which the simplest nonlinear process, two-photon X-ray absorption (TPA), can be observed. From these measurements we can extract the probability of this process as a function of photon energy and confirm both the nature and sub-femtosecond lifetime of the virtual intermediate electronic state.

Published
2016
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3. Establishing nonlinearity thresholds with ultraintense X-ray pulses

Author

Szlachetko, Jakub, Hoszowska, Joanna, Dousse, Jean-Claude, Nachtegaal, Maarten, Blachucki, Wojciech, Kayser, Yves, Sá, Jacinto, Messerschmidt, Marc, Boutet, Sebastien, Williams, Garth J., David, Christian, Smolentsev, Grigory, van Bokhoven, Jeroen A., Patterson, Bruce D., Penfold, Thomas J., Knopp, Gregor, Pajek, Marek, Abela, Rafael, Milne, Christopher J., Szlachetko, Jakub, Hoszowska, Joanna, Dousse, Jean-Claude, Nachtegaal, Maarten, Blachucki, Wojciech, Kayser, Yves, Sá, Jacinto, Messerschmidt, Marc, Boutet, Sebastien, Williams, Garth J., David, Christian, Smolentsev, Grigory, van Bokhoven, Jeroen A., Patterson, Bruce D., Penfold, Thomas J., Knopp, Gregor, Pajek, Marek, Abela, Rafael, and Milne, Christopher J.

Abstract

X-ray techniques have evolved over decades to become highly refined tools for a broad range of investigations. Importantly, these approaches rely on X-ray measurements that depend linearly on the number of incident X-ray photons. The advent of X-ray free electron lasers (XFELs) is opening the ability to reach extremely high photon numbers within ultrashort X-ray pulse durations and is leading to a paradigm shift in our ability to explore nonlinear X-ray signals. However, the enormous increase in X-ray peak power is a double-edged sword with new and exciting methods being developed but at the same time well-established techniques proving unreliable. Consequently, accurate knowledge about the threshold for nonlinear X-ray signals is essential. Herein we report an X-ray spectroscopic study that reveals important details on the thresholds for nonlinear X-ray interactions. By varying both the incident X-ray intensity and photon energy, we establish the regimes at which the simplest nonlinear process, two-photon X-ray absorption (TPA), can be observed. From these measurements we can extract the probability of this process as a function of photon energy and confirm both the nature and sub-femtosecond lifetime of the virtual intermediate electronic state.

Published
2016
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4. Establishing nonlinearity thresholds with ultraintense X-ray pulses

Author

Szlachetko, Jakub, Hoszowska, Joanna, Dousse, Jean-Claude, Nachtegaal, Maarten, Blachucki, Wojciech, Kayser, Yves, Sá, Jacinto, Messerschmidt, Marc, Boutet, Sebastien, Williams, Garth J., David, Christian, Smolentsev, Grigory, van Bokhoven, Jeroen A., Patterson, Bruce D., Penfold, Thomas J., Knopp, Gregor, Pajek, Marek, Abela, Rafael, Milne, Christopher J., Szlachetko, Jakub, Hoszowska, Joanna, Dousse, Jean-Claude, Nachtegaal, Maarten, Blachucki, Wojciech, Kayser, Yves, Sá, Jacinto, Messerschmidt, Marc, Boutet, Sebastien, Williams, Garth J., David, Christian, Smolentsev, Grigory, van Bokhoven, Jeroen A., Patterson, Bruce D., Penfold, Thomas J., Knopp, Gregor, Pajek, Marek, Abela, Rafael, and Milne, Christopher J.

Abstract

X-ray techniques have evolved over decades to become highly refined tools for a broad range of investigations. Importantly, these approaches rely on X-ray measurements that depend linearly on the number of incident X-ray photons. The advent of X-ray free electron lasers (XFELs) is opening the ability to reach extremely high photon numbers within ultrashort X-ray pulse durations and is leading to a paradigm shift in our ability to explore nonlinear X-ray signals. However, the enormous increase in X-ray peak power is a double-edged sword with new and exciting methods being developed but at the same time well-established techniques proving unreliable. Consequently, accurate knowledge about the threshold for nonlinear X-ray signals is essential. Herein we report an X-ray spectroscopic study that reveals important details on the thresholds for nonlinear X-ray interactions. By varying both the incident X-ray intensity and photon energy, we establish the regimes at which the simplest nonlinear process, two-photon X-ray absorption (TPA), can be observed. From these measurements we can extract the probability of this process as a function of photon energy and confirm both the nature and sub-femtosecond lifetime of the virtual intermediate electronic state.

Published
2016
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5. Establishing nonlinearity thresholds with ultraintense X-ray pulses

Author

Szlachetko, Jakub, Hoszowska, Joanna, Dousse, Jean-Claude, Nachtegaal, Maarten, Blachucki, Wojciech, Kayser, Yves, Sá, Jacinto, Messerschmidt, Marc, Boutet, Sebastien, Williams, Garth J., David, Christian, Smolentsev, Grigory, van Bokhoven, Jeroen A., Patterson, Bruce D., Penfold, Thomas J., Knopp, Gregor, Pajek, Marek, Abela, Rafael, Milne, Christopher J., Szlachetko, Jakub, Hoszowska, Joanna, Dousse, Jean-Claude, Nachtegaal, Maarten, Blachucki, Wojciech, Kayser, Yves, Sá, Jacinto, Messerschmidt, Marc, Boutet, Sebastien, Williams, Garth J., David, Christian, Smolentsev, Grigory, van Bokhoven, Jeroen A., Patterson, Bruce D., Penfold, Thomas J., Knopp, Gregor, Pajek, Marek, Abela, Rafael, and Milne, Christopher J.

Abstract

X-ray techniques have evolved over decades to become highly refined tools for a broad range of investigations. Importantly, these approaches rely on X-ray measurements that depend linearly on the number of incident X-ray photons. The advent of X-ray free electron lasers (XFELs) is opening the ability to reach extremely high photon numbers within ultrashort X-ray pulse durations and is leading to a paradigm shift in our ability to explore nonlinear X-ray signals. However, the enormous increase in X-ray peak power is a double-edged sword with new and exciting methods being developed but at the same time well-established techniques proving unreliable. Consequently, accurate knowledge about the threshold for nonlinear X-ray signals is essential. Herein we report an X-ray spectroscopic study that reveals important details on the thresholds for nonlinear X-ray interactions. By varying both the incident X-ray intensity and photon energy, we establish the regimes at which the simplest nonlinear process, two-photon X-ray absorption (TPA), can be observed. From these measurements we can extract the probability of this process as a function of photon energy and confirm both the nature and sub-femtosecond lifetime of the virtual intermediate electronic state.

Published
2016
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6. Establishing nonlinearity thresholds with ultraintense X-ray pulses

Author

Szlachetko, Jakub, Hoszowska, Joanna, Dousse, Jean-Claude, Nachtegaal, Maarten, Blachucki, Wojciech, Kayser, Yves, Sá, Jacinto, Messerschmidt, Marc, Boutet, Sebastien, Williams, Garth J., David, Christian, Smolentsev, Grigory, van Bokhoven, Jeroen A., Patterson, Bruce D., Penfold, Thomas J., Knopp, Gregor, Pajek, Marek, Abela, Rafael, Milne, Christopher J., Szlachetko, Jakub, Hoszowska, Joanna, Dousse, Jean-Claude, Nachtegaal, Maarten, Blachucki, Wojciech, Kayser, Yves, Sá, Jacinto, Messerschmidt, Marc, Boutet, Sebastien, Williams, Garth J., David, Christian, Smolentsev, Grigory, van Bokhoven, Jeroen A., Patterson, Bruce D., Penfold, Thomas J., Knopp, Gregor, Pajek, Marek, Abela, Rafael, and Milne, Christopher J.

Abstract

X-ray techniques have evolved over decades to become highly refined tools for a broad range of investigations. Importantly, these approaches rely on X-ray measurements that depend linearly on the number of incident X-ray photons. The advent of X-ray free electron lasers (XFELs) is opening the ability to reach extremely high photon numbers within ultrashort X-ray pulse durations and is leading to a paradigm shift in our ability to explore nonlinear X-ray signals. However, the enormous increase in X-ray peak power is a double-edged sword with new and exciting methods being developed but at the same time well-established techniques proving unreliable. Consequently, accurate knowledge about the threshold for nonlinear X-ray signals is essential. Herein we report an X-ray spectroscopic study that reveals important details on the thresholds for nonlinear X-ray interactions. By varying both the incident X-ray intensity and photon energy, we establish the regimes at which the simplest nonlinear process, two-photon X-ray absorption (TPA), can be observed. From these measurements we can extract the probability of this process as a function of photon energy and confirm both the nature and sub-femtosecond lifetime of the virtual intermediate electronic state.

Published
2016
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7. Structure of a photosynthetic reaction centre determined by serial femtosecond crystallography

Author

Johansson, Linda C., Arnlund, David, Katona, Gergely, White, Thomas A., Barty, Anton, DePonte, Daniel P., Shoeman, Robert L., Wickstrand, Cecilia, Sharma, Amit, Williams, Garth J., Aquila, Andrew, Bogan, Michael J., Caleman, Carl, Davidsson, Jan, Doak, R. Bruce, Frank, Matthias, Fromme, Raimund, Galli, Lorenzo, Grotjohann, Ingo, Hunter, Mark S., Kassemeyer, Stephan, Kirian, Richard A., Kupitz, Christopher, Liang, Mengning, Lomb, Lukas, Malmerberg, Erik, Martin, Andrew V., Messerschmidt, Marc, Nass, Karol, Redecke, Lars, Seibert, M. Marvin, Sjoehamn, Jennie, Steinbrener, Jan, Stellato, Francesco, Wang, Dingjie, Wahlgren, Weixaio Y., Weierstall, Uwe, Westenhoff, Sebastian, Zatsepin, Nadia A., Boutet, Sebastien, Spence, John C. H., Schlichting, Ilme, Chapman, Henry N., Fromme, Petra, Neutze, Richard, Johansson, Linda C., Arnlund, David, Katona, Gergely, White, Thomas A., Barty, Anton, DePonte, Daniel P., Shoeman, Robert L., Wickstrand, Cecilia, Sharma, Amit, Williams, Garth J., Aquila, Andrew, Bogan, Michael J., Caleman, Carl, Davidsson, Jan, Doak, R. Bruce, Frank, Matthias, Fromme, Raimund, Galli, Lorenzo, Grotjohann, Ingo, Hunter, Mark S., Kassemeyer, Stephan, Kirian, Richard A., Kupitz, Christopher, Liang, Mengning, Lomb, Lukas, Malmerberg, Erik, Martin, Andrew V., Messerschmidt, Marc, Nass, Karol, Redecke, Lars, Seibert, M. Marvin, Sjoehamn, Jennie, Steinbrener, Jan, Stellato, Francesco, Wang, Dingjie, Wahlgren, Weixaio Y., Weierstall, Uwe, Westenhoff, Sebastian, Zatsepin, Nadia A., Boutet, Sebastien, Spence, John C. H., Schlichting, Ilme, Chapman, Henry N., Fromme, Petra, and Neutze, Richard

Abstract

Serial femtosecond crystallography is an X-ray free-electron-laser-based method with considerable potential to have an impact on challenging problems in structural biology. Here we present X-ray diffraction data recorded from microcrystals of the Blastochloris viridis photosynthetic reaction centre to 2.8 angstrom resolution and determine its serial femtosecond crystallography structure to 3.5 angstrom resolution. Although every microcrystal is exposed to a dose of 33MGy, no signs of X-ray-induced radiation damage are visible in this integral membrane protein structure.

Published
2013
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8. Structure of a photosynthetic reaction centre determined by serial femtosecond crystallography

Author

Johansson, Linda C., Arnlund, David, Katona, Gergely, White, Thomas A., Barty, Anton, DePonte, Daniel P., Shoeman, Robert L., Wickstrand, Cecilia, Sharma, Amit, Williams, Garth J., Aquila, Andrew, Bogan, Michael J., Caleman, Carl, Davidsson, Jan, Doak, R. Bruce, Frank, Matthias, Fromme, Raimund, Galli, Lorenzo, Grotjohann, Ingo, Hunter, Mark S., Kassemeyer, Stephan, Kirian, Richard A., Kupitz, Christopher, Liang, Mengning, Lomb, Lukas, Malmerberg, Erik, Martin, Andrew V., Messerschmidt, Marc, Nass, Karol, Redecke, Lars, Seibert, M. Marvin, Sjoehamn, Jennie, Steinbrener, Jan, Stellato, Francesco, Wang, Dingjie, Wahlgren, Weixaio Y., Weierstall, Uwe, Westenhoff, Sebastian, Zatsepin, Nadia A., Boutet, Sebastien, Spence, John C. H., Schlichting, Ilme, Chapman, Henry N., Fromme, Petra, Neutze, Richard, Johansson, Linda C., Arnlund, David, Katona, Gergely, White, Thomas A., Barty, Anton, DePonte, Daniel P., Shoeman, Robert L., Wickstrand, Cecilia, Sharma, Amit, Williams, Garth J., Aquila, Andrew, Bogan, Michael J., Caleman, Carl, Davidsson, Jan, Doak, R. Bruce, Frank, Matthias, Fromme, Raimund, Galli, Lorenzo, Grotjohann, Ingo, Hunter, Mark S., Kassemeyer, Stephan, Kirian, Richard A., Kupitz, Christopher, Liang, Mengning, Lomb, Lukas, Malmerberg, Erik, Martin, Andrew V., Messerschmidt, Marc, Nass, Karol, Redecke, Lars, Seibert, M. Marvin, Sjoehamn, Jennie, Steinbrener, Jan, Stellato, Francesco, Wang, Dingjie, Wahlgren, Weixaio Y., Weierstall, Uwe, Westenhoff, Sebastian, Zatsepin, Nadia A., Boutet, Sebastien, Spence, John C. H., Schlichting, Ilme, Chapman, Henry N., Fromme, Petra, and Neutze, Richard

Abstract

Serial femtosecond crystallography is an X-ray free-electron-laser-based method with considerable potential to have an impact on challenging problems in structural biology. Here we present X-ray diffraction data recorded from microcrystals of the Blastochloris viridis photosynthetic reaction centre to 2.8 angstrom resolution and determine its serial femtosecond crystallography structure to 3.5 angstrom resolution. Although every microcrystal is exposed to a dose of 33MGy, no signs of X-ray-induced radiation damage are visible in this integral membrane protein structure.

Published
2013
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9. Structure of a photosynthetic reaction centre determined by serial femtosecond crystallography

Author

Johansson, Linda C., Arnlund, David, Katona, Gergely, White, Thomas A., Barty, Anton, DePonte, Daniel P., Shoeman, Robert L., Wickstrand, Cecilia, Sharma, Amit, Williams, Garth J., Aquila, Andrew, Bogan, Michael J., Caleman, Carl, Davidsson, Jan, Doak, R. Bruce, Frank, Matthias, Fromme, Raimund, Galli, Lorenzo, Grotjohann, Ingo, Hunter, Mark S., Kassemeyer, Stephan, Kirian, Richard A., Kupitz, Christopher, Liang, Mengning, Lomb, Lukas, Malmerberg, Erik, Martin, Andrew V., Messerschmidt, Marc, Nass, Karol, Redecke, Lars, Seibert, M. Marvin, Sjoehamn, Jennie, Steinbrener, Jan, Stellato, Francesco, Wang, Dingjie, Wahlgren, Weixaio Y., Weierstall, Uwe, Westenhoff, Sebastian, Zatsepin, Nadia A., Boutet, Sebastien, Spence, John C. H., Schlichting, Ilme, Chapman, Henry N., Fromme, Petra, Neutze, Richard, Johansson, Linda C., Arnlund, David, Katona, Gergely, White, Thomas A., Barty, Anton, DePonte, Daniel P., Shoeman, Robert L., Wickstrand, Cecilia, Sharma, Amit, Williams, Garth J., Aquila, Andrew, Bogan, Michael J., Caleman, Carl, Davidsson, Jan, Doak, R. Bruce, Frank, Matthias, Fromme, Raimund, Galli, Lorenzo, Grotjohann, Ingo, Hunter, Mark S., Kassemeyer, Stephan, Kirian, Richard A., Kupitz, Christopher, Liang, Mengning, Lomb, Lukas, Malmerberg, Erik, Martin, Andrew V., Messerschmidt, Marc, Nass, Karol, Redecke, Lars, Seibert, M. Marvin, Sjoehamn, Jennie, Steinbrener, Jan, Stellato, Francesco, Wang, Dingjie, Wahlgren, Weixaio Y., Weierstall, Uwe, Westenhoff, Sebastian, Zatsepin, Nadia A., Boutet, Sebastien, Spence, John C. H., Schlichting, Ilme, Chapman, Henry N., Fromme, Petra, and Neutze, Richard

Abstract

Serial femtosecond crystallography is an X-ray free-electron-laser-based method with considerable potential to have an impact on challenging problems in structural biology. Here we present X-ray diffraction data recorded from microcrystals of the Blastochloris viridis photosynthetic reaction centre to 2.8 angstrom resolution and determine its serial femtosecond crystallography structure to 3.5 angstrom resolution. Although every microcrystal is exposed to a dose of 33MGy, no signs of X-ray-induced radiation damage are visible in this integral membrane protein structure.

Published
2013
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10. Structure of a photosynthetic reaction centre determined by serial femtosecond crystallography

Author

Johansson, Linda C., Arnlund, David, Katona, Gergely, White, Thomas A., Barty, Anton, DePonte, Daniel P., Shoeman, Robert L., Wickstrand, Cecilia, Sharma, Amit, Williams, Garth J., Aquila, Andrew, Bogan, Michael J., Caleman, Carl, Davidsson, Jan, Doak, R. Bruce, Frank, Matthias, Fromme, Raimund, Galli, Lorenzo, Grotjohann, Ingo, Hunter, Mark S., Kassemeyer, Stephan, Kirian, Richard A., Kupitz, Christopher, Liang, Mengning, Lomb, Lukas, Malmerberg, Erik, Martin, Andrew V., Messerschmidt, Marc, Nass, Karol, Redecke, Lars, Seibert, M. Marvin, Sjoehamn, Jennie, Steinbrener, Jan, Stellato, Francesco, Wang, Dingjie, Wahlgren, Weixaio Y., Weierstall, Uwe, Westenhoff, Sebastian, Zatsepin, Nadia A., Boutet, Sebastien, Spence, John C. H., Schlichting, Ilme, Chapman, Henry N., Fromme, Petra, Neutze, Richard, Johansson, Linda C., Arnlund, David, Katona, Gergely, White, Thomas A., Barty, Anton, DePonte, Daniel P., Shoeman, Robert L., Wickstrand, Cecilia, Sharma, Amit, Williams, Garth J., Aquila, Andrew, Bogan, Michael J., Caleman, Carl, Davidsson, Jan, Doak, R. Bruce, Frank, Matthias, Fromme, Raimund, Galli, Lorenzo, Grotjohann, Ingo, Hunter, Mark S., Kassemeyer, Stephan, Kirian, Richard A., Kupitz, Christopher, Liang, Mengning, Lomb, Lukas, Malmerberg, Erik, Martin, Andrew V., Messerschmidt, Marc, Nass, Karol, Redecke, Lars, Seibert, M. Marvin, Sjoehamn, Jennie, Steinbrener, Jan, Stellato, Francesco, Wang, Dingjie, Wahlgren, Weixaio Y., Weierstall, Uwe, Westenhoff, Sebastian, Zatsepin, Nadia A., Boutet, Sebastien, Spence, John C. H., Schlichting, Ilme, Chapman, Henry N., Fromme, Petra, and Neutze, Richard

Abstract

Serial femtosecond crystallography is an X-ray free-electron-laser-based method with considerable potential to have an impact on challenging problems in structural biology. Here we present X-ray diffraction data recorded from microcrystals of the Blastochloris viridis photosynthetic reaction centre to 2.8 angstrom resolution and determine its serial femtosecond crystallography structure to 3.5 angstrom resolution. Although every microcrystal is exposed to a dose of 33MGy, no signs of X-ray-induced radiation damage are visible in this integral membrane protein structure.

Published
2013
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11. Structure of a photosynthetic reaction centre determined by serial femtosecond crystallography

Author

Johansson, Linda C., Arnlund, David, Katona, Gergely, White, Thomas A., Barty, Anton, DePonte, Daniel P., Shoeman, Robert L., Wickstrand, Cecilia, Sharma, Amit, Williams, Garth J., Aquila, Andrew, Bogan, Michael J., Caleman, Carl, Davidsson, Jan, Doak, R. Bruce, Frank, Matthias, Fromme, Raimund, Galli, Lorenzo, Grotjohann, Ingo, Hunter, Mark S., Kassemeyer, Stephan, Kirian, Richard A., Kupitz, Christopher, Liang, Mengning, Lomb, Lukas, Malmerberg, Erik, Martin, Andrew V., Messerschmidt, Marc, Nass, Karol, Redecke, Lars, Seibert, M. Marvin, Sjoehamn, Jennie, Steinbrener, Jan, Stellato, Francesco, Wang, Dingjie, Wahlgren, Weixaio Y., Weierstall, Uwe, Westenhoff, Sebastian, Zatsepin, Nadia A., Boutet, Sebastien, Spence, John C. H., Schlichting, Ilme, Chapman, Henry N., Fromme, Petra, Neutze, Richard, Johansson, Linda C., Arnlund, David, Katona, Gergely, White, Thomas A., Barty, Anton, DePonte, Daniel P., Shoeman, Robert L., Wickstrand, Cecilia, Sharma, Amit, Williams, Garth J., Aquila, Andrew, Bogan, Michael J., Caleman, Carl, Davidsson, Jan, Doak, R. Bruce, Frank, Matthias, Fromme, Raimund, Galli, Lorenzo, Grotjohann, Ingo, Hunter, Mark S., Kassemeyer, Stephan, Kirian, Richard A., Kupitz, Christopher, Liang, Mengning, Lomb, Lukas, Malmerberg, Erik, Martin, Andrew V., Messerschmidt, Marc, Nass, Karol, Redecke, Lars, Seibert, M. Marvin, Sjoehamn, Jennie, Steinbrener, Jan, Stellato, Francesco, Wang, Dingjie, Wahlgren, Weixaio Y., Weierstall, Uwe, Westenhoff, Sebastian, Zatsepin, Nadia A., Boutet, Sebastien, Spence, John C. H., Schlichting, Ilme, Chapman, Henry N., Fromme, Petra, and Neutze, Richard

Abstract

Serial femtosecond crystallography is an X-ray free-electron-laser-based method with considerable potential to have an impact on challenging problems in structural biology. Here we present X-ray diffraction data recorded from microcrystals of the Blastochloris viridis photosynthetic reaction centre to 2.8 angstrom resolution and determine its serial femtosecond crystallography structure to 3.5 angstrom resolution. Although every microcrystal is exposed to a dose of 33MGy, no signs of X-ray-induced radiation damage are visible in this integral membrane protein structure.

Published
2013
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12. In vivo protein crystallization opens new routes in structural biology

Author

Koopmann, Rudolf, Cupelli, Karolina, Redecke, Lars, Nass, Karol, DePonte, Daniel P, White, Thomas A, Stellato, Francesco, Rehders, Dirk, Liang, Mengning, Andreasson, Jakob, Aquila, Andrew, Bajt, Sasa, Barthelmess, Miriam, Barty, Anton, Bogan, Michael J, Bostedt, Christoph, Boutet, Sebastien, Bozek, John D, Caleman, Carl, Coppola, Nicola, Davidsson, Jan, Doak, R Bruce, Ekeberg, Tomas, Epp, Sascha W, Erk, Benjamin, Fleckenstein, Holger, Foucar, Lutz, Graafsma, Heinz, Gumprecht, Lars, Hajdu, Janos, Hampton, Christina Y, Hartmann, Andreas, Hartmann, Robert, Hauser, Gunter, Hirsemann, Helmut, Holl, Peter, Hunter, Mark S, Kassemeyer, Stephan, Kirian, Richard A, Lomb, Lukas, Maia, Filipe R N C, Kimmel, Nils, Martin, Andrew V, Messerschmidt, Marc, Reich, Christian, Rolles, Daniel, Rudek, Benedikt, Rudenko, Artem, Schlichting, Ilme, Schulz, Joachim, Seibert, M Marvin, Shoeman, Robert L, Sierra, Raymond G, Soltau, Heike, Stern, Stephan, Struder, Lothar, Timneanu, Nicusor, Ullrich, Joachim, Wang, Xiaoyu, Weidenspointner, Georg, Weierstall, Uwe, Williams, Garth J, Wunderer, Cornelia B, Fromme, Petra, Spence, John C H, Stehle, Thilo, Chapman, Henry N, Betzel, Christian, Duszenko, Michael, Koopmann, Rudolf, Cupelli, Karolina, Redecke, Lars, Nass, Karol, DePonte, Daniel P, White, Thomas A, Stellato, Francesco, Rehders, Dirk, Liang, Mengning, Andreasson, Jakob, Aquila, Andrew, Bajt, Sasa, Barthelmess, Miriam, Barty, Anton, Bogan, Michael J, Bostedt, Christoph, Boutet, Sebastien, Bozek, John D, Caleman, Carl, Coppola, Nicola, Davidsson, Jan, Doak, R Bruce, Ekeberg, Tomas, Epp, Sascha W, Erk, Benjamin, Fleckenstein, Holger, Foucar, Lutz, Graafsma, Heinz, Gumprecht, Lars, Hajdu, Janos, Hampton, Christina Y, Hartmann, Andreas, Hartmann, Robert, Hauser, Gunter, Hirsemann, Helmut, Holl, Peter, Hunter, Mark S, Kassemeyer, Stephan, Kirian, Richard A, Lomb, Lukas, Maia, Filipe R N C, Kimmel, Nils, Martin, Andrew V, Messerschmidt, Marc, Reich, Christian, Rolles, Daniel, Rudek, Benedikt, Rudenko, Artem, Schlichting, Ilme, Schulz, Joachim, Seibert, M Marvin, Shoeman, Robert L, Sierra, Raymond G, Soltau, Heike, Stern, Stephan, Struder, Lothar, Timneanu, Nicusor, Ullrich, Joachim, Wang, Xiaoyu, Weidenspointner, Georg, Weierstall, Uwe, Williams, Garth J, Wunderer, Cornelia B, Fromme, Petra, Spence, John C H, Stehle, Thilo, Chapman, Henry N, Betzel, Christian, and Duszenko, Michael

Abstract

Protein crystallization in cells has been observed several times in nature. However, owing to their small size these crystals have not yet been used for X-ray crystallographic analysis. We prepared nano-sized in vivo–grown crystals of Trypanosoma brucei enzymes and applied the emerging method of free-electron laser-based serial femtosecond crystallography to record interpretable diffraction data. This combined approach will open new opportunities in structural systems biology.

Published
2012
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