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Shigella dysenteriae as the primary cause of epidemic diarrhea have been reported. Shigellosis is an infectious disease caused by Shigella. The IpaD protein is one of the most important virulence factors in Shigella. For production of recombinant protein, coding sequence of ipaD+stxB cloned to pET28(a) exprsson vector and cloned gene confimed by PCR and restriction enzyme digestions. Coding sequence expressed in E. coli (BL21) was confirmed by SDS-PAGE, ELISA and Western blot. The recombinant protein accounted for about 54.87% of total bacterial proteins. Plant exprssion vector constructed via Golden Braid method. Transient expression of ipaD+stxB in lettuce performed with agrobacterium C58 strain and was confirmed at the level of transcriptome and protein through RT-PCR and ELISA assay. [ABSTRACT FROM AUTHOR]

The increased areas under cultivation of genetically modified plants have proponents and opponents in the international arena. According to potentially usefulness and harms of these products, organizations have established regulatory rules to safety use of them for feeding humans and livestock. Hence, genetically modified products have been distinguished from non-GMOs through labeling. Two more applicable genetic elements in plant transformations, 35S promoter and nos terminator, were considered as targeted elements for detection of GMO products in this research. Three maize grain cultivars, one soybean meal sample, and three bird’s food samples were used as experimental materials. The two-targeted genetic elements were detected in all samples. The Invertase and Lectin sequences as endogens were used for determining the presence of corn and soybean genomes as criteria. The two endogens were identified in all those of samples, which have the related genomes. In accordance to accepting of Cartagena Protocol in Iran, it’s been hopped that labeling rules of GMO products follow more seriously. [ABSTRACT FROM AUTHOR]