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Fish assemblages have been widely used as ecological indicators for assessing the level of environmental degradation and ecosystem health. Environmental disturbances affect the aquatic community structure in terms of abundance and biomass. Therefore, we tested the utility of abundance/biomass comparison (ABC) method, originally developed for marine ecosystems, to detect the anthropogenic disturbance in lotic systems using fish community data. Electrofishing was conducted in the period between 2003 and 2011 at 35 sites along the Southern Morava River basin. The results indicated that species richness strongly influences the utility of ABC method to detect the anthropogenic disturbance in lotic systems. The Warwick (W) statistic showed the positive correlation and the expected direction of response with some factors defining environmental quality, applying it on the samples with greater species richness. This approach has significant power for detecting environmental quality disturbance but may be limited due to effects of habitat variability in riverine environments.

This paper presents the first checklist of the family Chironomidae (Diptera) in Serbia, based on literature citations in two periods (1981/82 and 2010/11) and the unpublished data as well as material examined by the authors. Our survey ranges over the Central, Eastern, Southern and Southeastern parts of Serbia (Southern Morava River basin). We have listed 110 taxa distributed in following five subfamilies: Chironominae (39), Diamesinae (3), Orthocladiinae (48), Prodiamesinae (2) and Tanypodynae (16).

Based on a literature review, the different approaches in the water quality assessment using fish communities in freshwaters are summarized. Fish assemblage indicators, developed throughout the world, were reviewed and the main differences in methodologies, number of metrics and values are summarized. We have drawn attention to the methods used for designing a fish-based index with a particular focus on original developments in North America and its adaptations in many different regions and habitat types. The main obstacles for ecological assessment are scarce ecological information and the problem with the classification. The lack of knowledge is especially true for species assemblages in the relatively unexplored river basins of Europe, e.g. The Balkans peninsular.