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A probiotic product of kefir is widely consumed by human beings. The purpose of this research was to investigate the eff ects of kefir on blood parameters and intestinal flora in rats. A total of 24 female rats were used in this study. During 35 days of experimental period, rats were fed with a commercial diet and water was provided ad libitum. Kefir was given at the levels of 10 mL/kg, 20 mL/kg and 30 mL/kg with oral gavage to the first, second and third treatment groups, respectively. Kefir was not given to the control group. The number of yeast was found to be 1.65x107 and the number of lactobacilli was found to be 4x108 in kefir. At the end of the experiment, blood samples were taken from all rats. Blood plasma parameters and were investigated. The intestinal microfl ora was investigated by classical colony counting method. No diff erences were observed among the groups in total protein, albumin, uric acids, SGPT, SGOT, alkaline phosphatase and phosphorus in blood plasma. The plasma triglyceride and cholesterol levels in the second and third groups were lower than control group (P