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Young leaf segments from plants growing both in vivo and in vitro were cultured on Mitra et al medium (BM) supplemented variously with auxins (IAA, NAA, 2,4-D), kinetin (KN), GA3, and organic supplements (peptone, urea, casein hydrolysate, and yeast extract). The explants from mature leaves did not show any growth and turned necrotic, while those obtained from juvenile leaves of 6-mo-old plantlets growing in vitro developed protocorm-like bodies (plbs) at their cut surfaces within 4-7 wk, depending on the growth medium. Histological studies revealed their epidermal origin. Formation of plbs subsequently spread all along the explant and suggests a meristematic/generative potential of the epidermal cells in immature leaves. Upon subculture in basal medium, the plbs differentiated into plantlets within 4-5 wk. Although regeneration of plbs was achieved in BM and the auxins or GA3, KN in combination with IAA/NAA in the presence of peptone accelerated their formation.