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This study analyzed MeHg in a fast, simple, low-waste, and accurate by using ultra-high liquid chromatography coupled with inductively coupled plasma mass spectrometry. Simple preparation by liquid extraction with sonication at room temperature was effective extract MeHg from Certified Reference Material (CRM) and shark meat samples. Effective MeHg separation was achieved in less than 300 s using a C18 Hypersil Gold analytical column with a mobile gradient phase of 0.5% (w/v) L-cysteine in 2% (v/v) HNO3 and 100% methanol. The MeHg was extracted from 100 mg of shark meat using 1 mL of 0.5% (w/v) L-cysteine in 2% (v/v) HNO3 and sonicated for 30 min. Analysis of certified reference material (DORM-4) showed values between the experimental and certified values. The observed limit of detection and quantification MeHg were 0.86 and 2.85 pg/L, respectively. This method was applied to measure MeHg in shark meat from Binuangeun areas. The MeHg concentration in Rhizoprionodon acutus was 0.22–0.63 mg/kg wet weight (w.w.), Squalus hemipinis 0.68–1.14 mg/kg w.w., and 0.29–1.22 mg/kg w.w. for Sphyrna lewini. This study provides a quick and easy method to evaluate MeHg in shark meat or other seafood products and applies to many samples in a single assay.

Metals accumulation in threatened sharks’ meat represents a global health issue. The objective of the current study was to measure the concentration of six metals (Li, Ti, Ni, Cd, As, and Pb) using ICP-MS in threatened sharks’ meat of Rhizoprionodon acutus, Squalus hemipinnis, and Sphyrna lewini from Binuangeun Fish Auction in Lebak, Banten, Indonesia. The results showed that the concentration of Ni, Li, Cd, and Pb was below the acceptable levels for food sources for human consumption, except for As concentration (more than 30 fold higher). This study showed high levels of Ti concentration in all threatened sharks' meat. Overall, this study shows that an accumulation of Ti in sharks' meat should be considered a risk to the health of seafood consumers.

The high level of fish consumption was the main factor in the vulnerability of Hg exposure to the human body. The preliminary information of the total mercury (THg) concentration of Indian scad (Decapterus russelli) and Torpedo scad (Megalaspis cordyla) from Binuangeun fish auction in Lebak, Banten, Indonesia, was presented in this research. The objective of this research was to understand the distribution of THg in each organ of D. russelli (muscle, gut tissues, gonad, and eggs) and M. cordyla (muscle, liver, and gut contents). The results showed that THg in all samples were below the National Regulation limit from the National Agency of Drug and Food in mg/kg ww. THg gonad from D. russelli was showed highly significant (P < 0.01) lower compare THg in muscle, gut tissues, and eggs. Pearson’s correlation from THg in each organ of D. russelli compare to total weight and total length showed that concentration of THg in gonad highly significantly (p < 0.01) correlated with total body weight (r2 = 0.97) and total length (r2 = 0.96). M. cordyla was showed no correlation of THg in muscle compare to total length and total weight. The present study showed that D. russelli could accumulate higher mercury compare to M. cordyla and need caution while consuming this fish.

The concentration of nineteen metals (Hg, As, Cd, Co, Cr, Cu, Fe, Li, Mg, Mn, Mo, Ni, Pb, Se, Sr, Ti, Tl, V, and Zn) were determined in muscle tissues and digestive contents of Periglypta reticulata (Kerang Geton), collected from Lancang Island part of Seribu Islands, Jakarta. An interaction between toxic and essential metal in a clam is also studied. The results showed high concentrations of As (4.56), V (1.20), and Zn (4.91) mg/kg wet weight in muscle tissues and As (7.16), Ti (2.53), and Zn (8.68) mg/kg wet weight in digestive contents. Average concentrations of metals in muscle tissues and digestive contents were below regulation limit from permissible standard National Agency of Drug and Food Control except for Arsenic (As). The average concentration of metals in muscle tissues was significantly (P < 0.05) lower than in digestive contents except As, Co, Cr, Mg, and Tl, respectively. Toxic metals (Pb, Hg, Cd, and As) showed a strong correlation with several essential metals so that these metals can be a threat to the main function of a particular metal. The present study showed digestive contents could accumulate in higher metals; therefore, we suggested removing it before consuming this clam.

The high level of fish consumption was the main factor in the vulnerability of Hg exposure to the human body. The preliminary information of the total mercury (THg) concentration of Indian scad (Decapterus russelli) and Torpedo scad (Megalaspis cordyla) from Binuangeun fish auction in Lebak, Banten, Indonesia, was presented in this research. The objective of this research was to understand the distribution of THg in each organ of D. russelli (muscle, gut tissues, gonad, and eggs) and M. cordyla (muscle, liver, and gut contents). The results showed that THg in all samples were below the National Regulation limit from the National Agency of Drug and Food in mg/kg ww. THg gonad from D. russelli was showed highly significant (P < 0.01) lower compare THg in muscle, gut tissues, and eggs. Pearson’s correlation from THg in each organ of D. russelli compare to total weight and total length showed that concentration of THg in gonad highly significantly (p < 0.01) correlated with total body weight (r2 = 0.97) and total length (r2 = 0.96). M. cordyla was showed no correlation of THg in muscle compare to total length and total weight. The present study showed that D. russelli could accumulate higher mercury compare to M. cordyla and need caution while consuming this fish.

In Copper (Cu) based antifouling (AF) paints Cu was largely used as booster biocide after organotin was banned. Cu is micronutrient which is important in photosynthesis process because Cu is an essential metal as component of enzyme and electron transport chain. But in certain dosage, Cu could be toxic to marine organism. Chaetoceros gracilis and Isochrysis sp. are dominant microalgae in aquatic ecosystem. In this study the effect of Cu and Cadmium (Cd) on two marine microalgae, C. gracilis and Isochrysis sp. were compared. Toxicity test was based on American Standard for Testing Material (ASTM). IC 50 -96 h of Cd as reference toxicant was 2,370 m g.L -1 for C. gracilis and 490 m g.L -1 for Isochrysis sp. IC 50 -96 h of Cu to growth of C. gracilis was 63.75 m g.L -1 and Isochrysis sp. was 31.80 m g.L -1 . Both Cd and Cu were inhibited growth of microalgae. Based on IC 50 -96 h value, it could be concluded that Cu was more toxic than Cd. Toxicity of Cu was 37 times stronger than Cd for C. gracilis and 15 times for Isochrysis sp. It was estimated that at concentration 10 m g.L -1 Cu does not show observable effect (NOEC) to C. gracilis and 5 m g.L -1 to Isochrysis sp. The lowest observable effect of Cu (LOEC) to C. gracilis was at concentration 17 m g.L -1 and 10 m g.L -1 for Isochrysis sp.